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The map label for this gene is fliF [H]

Identifier: 170751440

GI number: 170751440

Start: 5373307

End: 5374884

Strand: Reverse

Name: fliF [H]

Synonym: Mrad2831_5059

Alternate gene names: 170751440

Gene position: 5374884-5373307 (Counterclockwise)

Preceding gene: 170751441

Following gene: 170751439

Centisome position: 88.43

GC content: 71.29

Gene sequence:

>1578_bases
ATGGCGGCGGTGACGCTGACGCTCATCGGCTTCTTCGCCTTCATCATCCTGCGGGTCTCGCGCCCCGATATGGGCGTGCT
GTTCTCCGACCTGTCGATGCAGGACTCCGCGTCCGTGGTCCGGGAATTGGACAGCCGCGGGATCCGCTACGAGACGCGGG
GCGATGCCGGGCAGACGATCCTGGCCCCGCGCCCGGACCTCGCCAAGCTGCGCATGGACCTCGCCGGCAAGGGGCTGCCG
ACCCAGGGCGGCGTCGGCTACGAGATCTTCGACAAGGGCGACGCGTTCTCATCCACCAACTTCGTTCAGAACGTGAATCA
CCTGCGCGCCCTGGAGGGCGAGCTGGCGCGCTCGATCCGGGCGATCGGCCGGGTCCAGGCGGCCCGTGTCCACCTCGTCC
TGCCGGAGCGCCGCCTGTTCGAGCGGGACCGGGAAAGCCCGAGCGCGGCGATCGTGCTGAAGCTGATGGGCGACCTCGAT
GCCGGGCAGGTCCGGGCGATCCGCCATCTCGTGGCCTCCGCCGTGGAGGGCCTCAAGCCCGAGCGGATCTCGATCGTCGA
CGAGCGCGGCCGGCTCCTGGCGGACGGCGCGCGGGATGCCGCGACCGACGTCGCCAGCGGGTTCGAGGACCGGCAGGTCG
CCCTCGAGCGGCGCCTGCGCGGCCAGATCGAGGACGTCGTCGCCGGCATCGTCGGCCCGGGCCGGGCCCGCGTCCAGGTC
AACGCGGAACTCGACCTCAACCGGGTCGAGAGCCGCTCCGAGACGTTCGATCCCGAGAGCCGCGTCGTGCGCTCCGCCCA
GACCCGCACCGAATCGGCGGTCACCGGCAATGCCGAGGGGCAGGTCAGCGTCGGCAACGAGCTGCCCGGCGCCAATGGCG
GCGACAAGGCGCCGACGCCCAAGGACGCGACCAACAAGAACGAGGAAACCACGAATTACGAGATCTCCCGGGTCACCAAG
ACCGAGATCGCCGAGGGCGGCCGGATCAAGCGCCTGTCGGTCGCGGTGGTGCTCGACGGGGTCTACGCCGCCGCGCCCGA
CGGCAAGCAGACCTATAGCCCGCGCACGCCCGCCGAGATCGAGCGGATCACCGCGCTGGTCCGCGGCGCGGTCGGTTTCG
ACAAGGCCCGCGGCGACCAGATCGAGGTGGTGAACCTGCGCTTCGCGGAAGCGCCGAGCGTGCCGCAATTCACCGAGCCG
ACGCTGATCGAGTCGCTGCTCGCCCCCACCAAGGACGACGTGCTGCGCATGGCCGAGCTCGCCGTCCTGGCGCTGCTGAC
CCTGATCGTCCTCCTCACGGTGGTCCGCCCCCTGGTGCGCCAGGTGCTCGCGCCGGTGCCGGAAGCGCCGGCCCTGACCG
CGTCGGGACTGGCGATCCCCGGCGACGCCGCCGTGGTCGCGGGTCCGGGCGGGACCGTGACGGTCATCGACCGCGACAGC
CCGACCGCGCGGCTGATGGAGTTCGCCAAGATCAACGGCCAGGTCCAGGCCGAGACGGTCCAGCGCGTGGTCGACATGGT
CCGGGCGAGCCCGTCCGAGACCGTCGAGGTGCTGCGCACCTGGATCCAGGAAGAGTGA

Upstream 100 bases:

>100_bases
GCGGACCGCCGGGGTCCGCCAGCGTTAGAGTGCAGCGAATCCTCCCGTGAAGCCGGTTCTCGATCTCGTCACGAAGTTCG
GACCGGCCCGAATCGCCGCC

Downstream 100 bases:

>100_bases
AGCGTAGGGCGTCGAGGGCAACACGATGGCGGCAGGTGCGGGACAGGGTCTGGCGAACACGCTGGCGACGGCGGGCGGCG
ATTCATTCGCGTCGATGCCC

Product: flagellar MS-ring protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 525; Mature: 524

Protein sequence:

>525_residues
MAAVTLTLIGFFAFIILRVSRPDMGVLFSDLSMQDSASVVRELDSRGIRYETRGDAGQTILAPRPDLAKLRMDLAGKGLP
TQGGVGYEIFDKGDAFSSTNFVQNVNHLRALEGELARSIRAIGRVQAARVHLVLPERRLFERDRESPSAAIVLKLMGDLD
AGQVRAIRHLVASAVEGLKPERISIVDERGRLLADGARDAATDVASGFEDRQVALERRLRGQIEDVVAGIVGPGRARVQV
NAELDLNRVESRSETFDPESRVVRSAQTRTESAVTGNAEGQVSVGNELPGANGGDKAPTPKDATNKNEETTNYEISRVTK
TEIAEGGRIKRLSVAVVLDGVYAAAPDGKQTYSPRTPAEIERITALVRGAVGFDKARGDQIEVVNLRFAEAPSVPQFTEP
TLIESLLAPTKDDVLRMAELAVLALLTLIVLLTVVRPLVRQVLAPVPEAPALTASGLAIPGDAAVVAGPGGTVTVIDRDS
PTARLMEFAKINGQVQAETVQRVVDMVRASPSETVEVLRTWIQEE

Sequences:

>Translated_525_residues
MAAVTLTLIGFFAFIILRVSRPDMGVLFSDLSMQDSASVVRELDSRGIRYETRGDAGQTILAPRPDLAKLRMDLAGKGLP
TQGGVGYEIFDKGDAFSSTNFVQNVNHLRALEGELARSIRAIGRVQAARVHLVLPERRLFERDRESPSAAIVLKLMGDLD
AGQVRAIRHLVASAVEGLKPERISIVDERGRLLADGARDAATDVASGFEDRQVALERRLRGQIEDVVAGIVGPGRARVQV
NAELDLNRVESRSETFDPESRVVRSAQTRTESAVTGNAEGQVSVGNELPGANGGDKAPTPKDATNKNEETTNYEISRVTK
TEIAEGGRIKRLSVAVVLDGVYAAAPDGKQTYSPRTPAEIERITALVRGAVGFDKARGDQIEVVNLRFAEAPSVPQFTEP
TLIESLLAPTKDDVLRMAELAVLALLTLIVLLTVVRPLVRQVLAPVPEAPALTASGLAIPGDAAVVAGPGGTVTVIDRDS
PTARLMEFAKINGQVQAETVQRVVDMVRASPSETVEVLRTWIQEE
>Mature_524_residues
AAVTLTLIGFFAFIILRVSRPDMGVLFSDLSMQDSASVVRELDSRGIRYETRGDAGQTILAPRPDLAKLRMDLAGKGLPT
QGGVGYEIFDKGDAFSSTNFVQNVNHLRALEGELARSIRAIGRVQAARVHLVLPERRLFERDRESPSAAIVLKLMGDLDA
GQVRAIRHLVASAVEGLKPERISIVDERGRLLADGARDAATDVASGFEDRQVALERRLRGQIEDVVAGIVGPGRARVQVN
AELDLNRVESRSETFDPESRVVRSAQTRTESAVTGNAEGQVSVGNELPGANGGDKAPTPKDATNKNEETTNYEISRVTKT
EIAEGGRIKRLSVAVVLDGVYAAAPDGKQTYSPRTPAEIERITALVRGAVGFDKARGDQIEVVNLRFAEAPSVPQFTEPT
LIESLLAPTKDDVLRMAELAVLALLTLIVLLTVVRPLVRQVLAPVPEAPALTASGLAIPGDAAVVAGPGGTVTVIDRDSP
TARLMEFAKINGQVQAETVQRVVDMVRASPSETVEVLRTWIQEE

Specific function: The M ring may be actively involved in energy transduction [H]

COG id: COG1766

COG function: function code NU; Flagellar biosynthesis/type III secretory pathway lipoprotein

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein. Bacterial flagellum basal body (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fliF family [H]

Homologues:

Organism=Escherichia coli, GI1788248, Length=434, Percent_Identity=35.0230414746544, Blast_Score=212, Evalue=5e-56,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013556
- InterPro:   IPR000067
- InterPro:   IPR006182 [H]

Pfam domain/function: PF01514 YscJ_FliF; PF08345 YscJ_FliF_C [H]

EC number: NA

Molecular weight: Translated: 56447; Mature: 56316

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAVTLTLIGFFAFIILRVSRPDMGVLFSDLSMQDSASVVRELDSRGIRYETRGDAGQTI
CCHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCHHHHHHHHHHHCCCEEEECCCCCCEE
LAPRPDLAKLRMDLAGKGLPTQGGVGYEIFDKGDAFSSTNFVQNVNHLRALEGELARSIR
ECCCCCHHHHHHHHCCCCCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
AIGRVQAARVHLVLPERRLFERDRESPSAAIVLKLMGDLDAGQVRAIRHLVASAVEGLKP
HHHHHHHEEEEEECCHHHHHHHCCCCCCCEEHHHHHCCCCCHHHHHHHHHHHHHHHCCCC
ERISIVDERGRLLADGARDAATDVASGFEDRQVALERRLRGQIEDVVAGIVGPGRARVQV
CCEEEECCCCCEEECCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEE
NAELDLNRVESRSETFDPESRVVRSAQTRTESAVTGNAEGQVSVGNELPGANGGDKAPTP
ECCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCCCCCC
KDATNKNEETTNYEISRVTKTEIAEGGRIKRLSVAVVLDGVYAAAPDGKQTYSPRTPAEI
CCCCCCCCCCCCEEEEHHHHHHHCCCCCEEEEEEEEEECCHHCCCCCCCCCCCCCCHHHH
ERITALVRGAVGFDKARGDQIEVVNLRFAEAPSVPQFTEPTLIESLLAPTKDDVLRMAEL
HHHHHHHHHHCCCCCCCCCEEEEEEEEECCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHH
AVLALLTLIVLLTVVRPLVRQVLAPVPEAPALTASGLAIPGDAAVVAGPGGTVTVIDRDS
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEECCCCCEEEECCCCEEEEEECCC
PTARLMEFAKINGQVQAETVQRVVDMVRASPSETVEVLRTWIQEE
CHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
AAVTLTLIGFFAFIILRVSRPDMGVLFSDLSMQDSASVVRELDSRGIRYETRGDAGQTI
CHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCHHHHHHHHHHHCCCEEEECCCCCCEE
LAPRPDLAKLRMDLAGKGLPTQGGVGYEIFDKGDAFSSTNFVQNVNHLRALEGELARSIR
ECCCCCHHHHHHHHCCCCCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
AIGRVQAARVHLVLPERRLFERDRESPSAAIVLKLMGDLDAGQVRAIRHLVASAVEGLKP
HHHHHHHEEEEEECCHHHHHHHCCCCCCCEEHHHHHCCCCCHHHHHHHHHHHHHHHCCCC
ERISIVDERGRLLADGARDAATDVASGFEDRQVALERRLRGQIEDVVAGIVGPGRARVQV
CCEEEECCCCCEEECCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEE
NAELDLNRVESRSETFDPESRVVRSAQTRTESAVTGNAEGQVSVGNELPGANGGDKAPTP
ECCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCCCCCC
KDATNKNEETTNYEISRVTKTEIAEGGRIKRLSVAVVLDGVYAAAPDGKQTYSPRTPAEI
CCCCCCCCCCCCEEEEHHHHHHHCCCCCEEEEEEEEEECCHHCCCCCCCCCCCCCCHHHH
ERITALVRGAVGFDKARGDQIEVVNLRFAEAPSVPQFTEPTLIESLLAPTKDDVLRMAEL
HHHHHHHHHHCCCCCCCCCEEEEEEEEECCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHH
AVLALLTLIVLLTVVRPLVRQVLAPVPEAPALTASGLAIPGDAAVVAGPGGTVTVIDRDS
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEECCCCCEEEECCCCEEEEEECCC
PTARLMEFAKINGQVQAETVQRVVDMVRASPSETVEVLRTWIQEE
CHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8002583; 11259647 [H]