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The map label for this gene is lpsJ [H]

Identifier: 170751381

GI number: 170751381

Start: 5322717

End: 5323379

Strand: Reverse

Name: lpsJ [H]

Synonym: Mrad2831_5000

Alternate gene names: 170751381

Gene position: 5323379-5322717 (Counterclockwise)

Preceding gene: 170751382

Following gene: 170751363

Centisome position: 87.59

GC content: 66.06

Gene sequence:

>663_bases
ATGGCCTGGACCCGCGAGCAGATGGCGGCGCGCGCCGCCAAGGAGCTGGAGGACGGCTTCTACGTGAACCTCGGCATCGG
CATCCCGACGCTGGTGTCGAACTACATCCCCGAGGGCATGTCGGTGCAGCTCCAGTCGGAGAACGGCATGCTGGGCATGG
GCCCCTTCCCGTACGAGGGCGAGGAGGACCCGGACCTGATCAACGCCGGCAAGCAGACGATCACGGCGCTGCCGACGACC
AGCTACTTCTCCTCGGCCGATTCCTTCGGGATGATCCGCGGCGGGCACATCAACCTGTCGATCCTCGGCGCCATGCAGGT
GGCCGGCAACGGCGACCTCGCGAACTGGATGATCCCCGGCAAGATGGTGAAGGGGATGGGCGGCGCCATGGACCTCGTGG
CGGGCGTGAAGCGCGTCGTCGTGGTGATGGAGCACGTTGCCAAGAACAAGGACGGCACCGAGAGCCCGAAGCTGCTCAAG
GCCTGCGACCTGCCGCTGACCGGCACCCGCGTGGTCGACTTGGTGATCACCGATCTCGGCGTGTTCGAGATCGACAAGAA
GGGCGACGGTGGCATGAGGCTGATCGAGCTCGCCGACGGCGTCACCGAGGACGAGATCCGCGGGAAGACCGAGGCCGACT
TCACGGTCGTCCTGAAGAACTGA

Upstream 100 bases:

>100_bases
GCGCATCGAGCAGCGCACTACGCGCAAGCGCGGCGACAGCACCCCGGCCGCGGCGGCCGGCGGCGGCACGCACTGATCGG
ACGGGAAGGGAGCGAGAACG

Downstream 100 bases:

>100_bases
CGCCCCTGGGCCCGGCGGCCGACGTCGCCGGGCCCGTCGCGGGACGCGTTACCGGGGTCGGACGACCGATCGTCGAACGG
TCCCGCGGACGCGGCCGACG

Product: 3-oxoacid CoA-transferase subunit B

Products: NA

Alternate protein names: OXCT B; Succinyl CoA:3-oxoacid CoA-transferase [H]

Number of amino acids: Translated: 220; Mature: 219

Protein sequence:

>220_residues
MAWTREQMAARAAKELEDGFYVNLGIGIPTLVSNYIPEGMSVQLQSENGMLGMGPFPYEGEEDPDLINAGKQTITALPTT
SYFSSADSFGMIRGGHINLSILGAMQVAGNGDLANWMIPGKMVKGMGGAMDLVAGVKRVVVVMEHVAKNKDGTESPKLLK
ACDLPLTGTRVVDLVITDLGVFEIDKKGDGGMRLIELADGVTEDEIRGKTEADFTVVLKN

Sequences:

>Translated_220_residues
MAWTREQMAARAAKELEDGFYVNLGIGIPTLVSNYIPEGMSVQLQSENGMLGMGPFPYEGEEDPDLINAGKQTITALPTT
SYFSSADSFGMIRGGHINLSILGAMQVAGNGDLANWMIPGKMVKGMGGAMDLVAGVKRVVVVMEHVAKNKDGTESPKLLK
ACDLPLTGTRVVDLVITDLGVFEIDKKGDGGMRLIELADGVTEDEIRGKTEADFTVVLKN
>Mature_219_residues
AWTREQMAARAAKELEDGFYVNLGIGIPTLVSNYIPEGMSVQLQSENGMLGMGPFPYEGEEDPDLINAGKQTITALPTTS
YFSSADSFGMIRGGHINLSILGAMQVAGNGDLANWMIPGKMVKGMGGAMDLVAGVKRVVVVMEHVAKNKDGTESPKLLKA
CDLPLTGTRVVDLVITDLGVFEIDKKGDGGMRLIELADGVTEDEIRGKTEADFTVVLKN

Specific function: SHORT-CHAIN FATTY ACIDS METABOLISM. [C]

COG id: COG2057

COG function: function code I; Acyl CoA:acetate/3-ketoacid CoA transferase, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-oxoacid CoA-transferase subunit B family [H]

Homologues:

Organism=Homo sapiens, GI4557817, Length=216, Percent_Identity=57.8703703703704, Blast_Score=249, Evalue=2e-66,
Organism=Homo sapiens, GI11545841, Length=213, Percent_Identity=57.2769953051643, Blast_Score=242, Evalue=2e-64,
Organism=Escherichia coli, GI1788552, Length=212, Percent_Identity=51.4150943396226, Blast_Score=213, Evalue=1e-56,
Organism=Caenorhabditis elegans, GI17531517, Length=214, Percent_Identity=56.0747663551402, Blast_Score=239, Evalue=8e-64,
Organism=Drosophila melanogaster, GI21358325, Length=213, Percent_Identity=59.6244131455399, Blast_Score=253, Evalue=5e-68,
Organism=Drosophila melanogaster, GI24655794, Length=213, Percent_Identity=59.6244131455399, Blast_Score=253, Evalue=7e-68,

Paralogues:

None

Copy number: 369 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012791
- InterPro:   IPR004165
- InterPro:   IPR004164 [H]

Pfam domain/function: PF01144 CoA_trans [H]

EC number: =2.8.3.5 [H]

Molecular weight: Translated: 23495; Mature: 23364

Theoretical pI: Translated: 4.45; Mature: 4.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
5.9 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
5.5 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAWTREQMAARAAKELEDGFYVNLGIGIPTLVSNYIPEGMSVQLQSENGMLGMGPFPYEG
CCCCHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHCCCCCEEEEECCCCEEECCCCCCCC
EEDPDLINAGKQTITALPTTSYFSSADSFGMIRGGHINLSILGAMQVAGNGDLANWMIPG
CCCCHHHHCCCCEEEECCCCHHHCCCCCCCEEECCEEEEEEEEEEEECCCCCEEEEECCH
KMVKGMGGAMDLVAGVKRVVVVMEHVAKNKDGTESPKLLKACDLPLTGTRVVDLVITDLG
HHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCCCHHEEEEEECCCC
VFEIDKKGDGGMRLIELADGVTEDEIRGKTEADFTVVLKN
EEEEECCCCCCEEEEEECCCCCHHHHCCCCCCCEEEEEEC
>Mature Secondary Structure 
AWTREQMAARAAKELEDGFYVNLGIGIPTLVSNYIPEGMSVQLQSENGMLGMGPFPYEG
CCCHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHCCCCCEEEEECCCCEEECCCCCCCC
EEDPDLINAGKQTITALPTTSYFSSADSFGMIRGGHINLSILGAMQVAGNGDLANWMIPG
CCCCHHHHCCCCEEEECCCCHHHCCCCCCCEEECCEEEEEEEEEEEECCCCCEEEEECCH
KMVKGMGGAMDLVAGVKRVVVVMEHVAKNKDGTESPKLLKACDLPLTGTRVVDLVITDLG
HHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCCCHHEEEEEECCCC
VFEIDKKGDGGMRLIELADGVTEDEIRGKTEADFTVVLKN
EEEEECCCCCCEEEEEECCCCCHHHHCCCCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9385134 [H]