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The map label for this gene is mcbR [H]

Identifier: 170751314

GI number: 170751314

Start: 5251132

End: 5251827

Strand: Reverse

Name: mcbR [H]

Synonym: Mrad2831_4931

Alternate gene names: 170751314

Gene position: 5251827-5251132 (Counterclockwise)

Preceding gene: 170751315

Following gene: 170751309

Centisome position: 86.41

GC content: 71.84

Gene sequence:

>696_bases
ATGTCCCCCACGGGCCCTGGACGTCCCGCACCCGACCGGCTGCGGCACAAGACCGTGGCGGCGGCCGTCGCGGATTCCCT
GCGCGAGCAGATCCTGAACGGGGATCTGCCGCCGGGGGTGCAGCTGCGCCAGGACGCCCTGGCGGAGGAGTTCGGGATCA
GCCGCATCCCGGTCCGCGAAGCCCTGCTGCAGCTGGAGGCCAGCAAGCTCGTGCGGATCGTGCCGCATCGCGGGGCGGTC
GTGACGGGCCTGTCGCTCCAGGAGGTCGAGGACATCTTCCAGCTGCGCGTTCAGCTCGAGCCGCAGCTGCTCCAGCTCTC
GGCACCGCACCTGACCCCCGACGACATCCGCGATCTCGACGCCCTCTCGAAGCAGTACGGCGCGGCGCTGGAGACCGGCC
AGGTCCTCCGATGGGGCGACCTGAACAGCCGCTTCCACCTCGACCTGATCCGGCACGCCGGCCGGCCGCGCTCCCTGGCG
ATCGTGTCCGCGCTCCTGCAGGATTGCGAGCGGCCGACGCGGCTGCAGATCTCGACCTCCGAGGACGCGACCCGCGCGGT
CAGGGAGCACGCGCAGATCCTGGCCCTGTGCGAGGCCGGCAAGTCCGACGAGGCGGCCGCCTGCCTGCGGCGCCATATCG
AGCATATCGGCGTCTCGCTCATCGCCCTCTACCGGGGCGCGCTCGTCGAGGCGTGA

Upstream 100 bases:

>100_bases
AACCTCAGGTTCATTCGGGACAATCTGGCATTGGACAGACGCGCGTTTTCCGGGCAAGAGGCTCCCTTGTATACATGTAT
ACTGTTCTCCTGAGGCACTG

Downstream 100 bases:

>100_bases
GGGGGCCCGCCCCCGCGCGGCCGGGTCAGTCCGGCTTGGCCGACCGGCCGCCGATCCGGCCCGGCAGACGGGTCAGGCGA
TCCTTCATCTCCAGCACCTT

Product: GntR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 231; Mature: 230

Protein sequence:

>231_residues
MSPTGPGRPAPDRLRHKTVAAAVADSLREQILNGDLPPGVQLRQDALAEEFGISRIPVREALLQLEASKLVRIVPHRGAV
VTGLSLQEVEDIFQLRVQLEPQLLQLSAPHLTPDDIRDLDALSKQYGAALETGQVLRWGDLNSRFHLDLIRHAGRPRSLA
IVSALLQDCERPTRLQISTSEDATRAVREHAQILALCEAGKSDEAAACLRRHIEHIGVSLIALYRGALVEA

Sequences:

>Translated_231_residues
MSPTGPGRPAPDRLRHKTVAAAVADSLREQILNGDLPPGVQLRQDALAEEFGISRIPVREALLQLEASKLVRIVPHRGAV
VTGLSLQEVEDIFQLRVQLEPQLLQLSAPHLTPDDIRDLDALSKQYGAALETGQVLRWGDLNSRFHLDLIRHAGRPRSLA
IVSALLQDCERPTRLQISTSEDATRAVREHAQILALCEAGKSDEAAACLRRHIEHIGVSLIALYRGALVEA
>Mature_230_residues
SPTGPGRPAPDRLRHKTVAAAVADSLREQILNGDLPPGVQLRQDALAEEFGISRIPVREALLQLEASKLVRIVPHRGAVV
TGLSLQEVEDIFQLRVQLEPQLLQLSAPHLTPDDIRDLDALSKQYGAALETGQVLRWGDLNSRFHLDLIRHAGRPRSLAI
VSALLQDCERPTRLQISTSEDATRAVREHAQILALCEAGKSDEAAACLRRHIEHIGVSLIALYRGALVEA

Specific function: Important for biofilm formation. Represses expression of mcbA by binding to its promoter region, which prevents colanic acid overproduction and mucoidy [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH gntR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081914, Length=148, Percent_Identity=29.0540540540541, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1787821, Length=194, Percent_Identity=23.7113402061856, Blast_Score=61, Evalue=7e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011711
- InterPro:   IPR000524
- InterPro:   IPR011991 [H]

Pfam domain/function: PF07729 FCD; PF00392 GntR [H]

EC number: NA

Molecular weight: Translated: 25312; Mature: 25181

Theoretical pI: Translated: 6.72; Mature: 6.72

Prosite motif: PS50949 HTH_GNTR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPTGPGRPAPDRLRHKTVAAAVADSLREQILNGDLPPGVQLRQDALAEEFGISRIPVRE
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCHHH
ALLQLEASKLVRIVPHRGAVVTGLSLQEVEDIFQLRVQLEPQLLQLSAPHLTPDDIRDLD
HHHHHHHHHHHHHCCCCCCEEECCCHHHHHHHHHHHHHCCHHHHHHCCCCCCHHHHHHHH
ALSKQYGAALETGQVLRWGDLNSRFHLDLIRHAGRPRSLAIVSALLQDCERPTRLQISTS
HHHHHHCCHHCCCCEEEECCCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCEEEEECC
EDATRAVREHAQILALCEAGKSDEAAACLRRHIEHIGVSLIALYRGALVEA
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SPTGPGRPAPDRLRHKTVAAAVADSLREQILNGDLPPGVQLRQDALAEEFGISRIPVRE
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCHHH
ALLQLEASKLVRIVPHRGAVVTGLSLQEVEDIFQLRVQLEPQLLQLSAPHLTPDDIRDLD
HHHHHHHHHHHHHCCCCCCEEECCCHHHHHHHHHHHHHCCHHHHHHCCCCCCHHHHHHHH
ALSKQYGAALETGQVLRWGDLNSRFHLDLIRHAGRPRSLAIVSALLQDCERPTRLQISTS
HHHHHHCCHHCCCCEEEECCCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCEEEEECC
EDATRAVREHAQILALCEAGKSDEAAACLRRHIEHIGVSLIALYRGALVEA
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]