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The map label for this gene is fliY [C]

Identifier: 170751307

GI number: 170751307

Start: 5244441

End: 5245226

Strand: Reverse

Name: fliY [C]

Synonym: Mrad2831_4924

Alternate gene names: 170751307

Gene position: 5245226-5244441 (Counterclockwise)

Preceding gene: 170751309

Following gene: 170751306

Centisome position: 86.3

GC content: 68.7

Gene sequence:

>786_bases
ATGCGATTTTTCTCTGCCAGCCGCGTCGCGGCGTTCGTCGTCGCGGCGCTCCTCGGCGCAGCCGCCAGCGCGCCCGCGAA
GGCCGACGATCTCGACGCGATTCGCGCCGCCAAGACCCTGCGCATCGGCACCGAGGGCACCTACGCGCCCTTCACGTTCC
ACGACGCGTCCGGGCAGCTCGTCGGCTTCGACGTGGAGATCGGCCGCAAGGTCGCCGAGCAGCTGGGCGTCACGCCGCAA
TTCCTCGAGGGCAAGTGGGACGGGCTGATCGCCGGGCTCGACGGCAAGCGCTACGACGTGGTCATCAACCAGGTCGGCAT
CACCAAGGAGCGGCAGGCGAAGTTCGACTTCTCCGAGCCCTACATCCGGGCGCGGGCCGTGCTGATCACGCGGGCGGACC
GGTCCGACATCAAGTCCTTCGCCGATCTCAAGGGCAAGAAGGCCGCCCAGAGCCTCTCCAGCAACTTCGCGCGCCTCGCC
GAGCAGTCCGGCGCCGAGCTCGTCGGGACGGACGGCTTCGACCAGTCGGTCCAGCTCGTCCTCACCGGCCGCGCCGACGC
CACCGTCAACGACAGCCTGTCGTTCCTGGACTTCCACAAGCAGAAGCCGAACGCCCCCGTGAAGATCGCCGCCGAGCAGG
CCGACGCGGACGCCTCCGGGATCATCATGCGCAAGAACAACCCGGCGCTGAAGGCCGCGATCGACAAGGCGCTGGAGGCG
ATCCGGGCCGACGGCAGCTACGCGGCGATCGCCCAGAAGTACTTCGGGGCCGACGTCTCCAAGTAA

Upstream 100 bases:

>100_bases
TTTCCTGTACGGCCGAGCTCTTCTTTGCTTTGTCCCTCGACAGCCGAGATCACGCGCGGCGGCCGCACGAGAACAGCGTT
TTCTTTTCGGAGCTACTGTA

Downstream 100 bases:

>100_bases
CCCGTCCGCGGCGCGCCCGCCCGGGCGCCGATCCCGCACAGCCCGCCGCTCGATCCGCCCGGTAGGAGACGCCGCCCCGT
GCCGCACTGGCTCGACCTCA

Product: extracellular solute-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 261; Mature: 261

Protein sequence:

>261_residues
MRFFSASRVAAFVVAALLGAAASAPAKADDLDAIRAAKTLRIGTEGTYAPFTFHDASGQLVGFDVEIGRKVAEQLGVTPQ
FLEGKWDGLIAGLDGKRYDVVINQVGITKERQAKFDFSEPYIRARAVLITRADRSDIKSFADLKGKKAAQSLSSNFARLA
EQSGAELVGTDGFDQSVQLVLTGRADATVNDSLSFLDFHKQKPNAPVKIAAEQADADASGIIMRKNNPALKAAIDKALEA
IRADGSYAAIAQKYFGADVSK

Sequences:

>Translated_261_residues
MRFFSASRVAAFVVAALLGAAASAPAKADDLDAIRAAKTLRIGTEGTYAPFTFHDASGQLVGFDVEIGRKVAEQLGVTPQ
FLEGKWDGLIAGLDGKRYDVVINQVGITKERQAKFDFSEPYIRARAVLITRADRSDIKSFADLKGKKAAQSLSSNFARLA
EQSGAELVGTDGFDQSVQLVLTGRADATVNDSLSFLDFHKQKPNAPVKIAAEQADADASGIIMRKNNPALKAAIDKALEA
IRADGSYAAIAQKYFGADVSK
>Mature_261_residues
MRFFSASRVAAFVVAALLGAAASAPAKADDLDAIRAAKTLRIGTEGTYAPFTFHDASGQLVGFDVEIGRKVAEQLGVTPQ
FLEGKWDGLIAGLDGKRYDVVINQVGITKERQAKFDFSEPYIRARAVLITRADRSDIKSFADLKGKKAAQSLSSNFARLA
EQSGAELVGTDGFDQSVQLVLTGRADATVNDSLSFLDFHKQKPNAPVKIAAEQADADASGIIMRKNNPALKAAIDKALEA
IRADGSYAAIAQKYFGADVSK

Specific function: Probably part of a binding-protein-dependent transport system for an amino acid [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788228, Length=267, Percent_Identity=38.2022471910112, Blast_Score=169, Evalue=2e-43,
Organism=Escherichia coli, GI1787085, Length=225, Percent_Identity=30.6666666666667, Blast_Score=114, Evalue=9e-27,
Organism=Escherichia coli, GI1788648, Length=233, Percent_Identity=30.4721030042918, Blast_Score=107, Evalue=9e-25,
Organism=Escherichia coli, GI1788649, Length=233, Percent_Identity=32.618025751073, Blast_Score=104, Evalue=5e-24,
Organism=Escherichia coli, GI1787031, Length=226, Percent_Identity=29.2035398230088, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1787088, Length=225, Percent_Identity=28, Blast_Score=97, Evalue=8e-22,

Paralogues:

None

Copy number: 1920 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1060 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 80 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 27816; Mature: 27816

Theoretical pI: Translated: 9.26; Mature: 9.26

Prosite motif: PS01039 SBP_BACTERIAL_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
0.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFFSASRVAAFVVAALLGAAASAPAKADDLDAIRAAKTLRIGTEGTYAPFTFHDASGQL
CCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHEEECCCCCCCCEEEECCCCCE
VGFDVEIGRKVAEQLGVTPQFLEGKWDGLIAGLDGKRYDVVINQVGITKERQAKFDFSEP
EEEEHHHHHHHHHHHCCCHHHHCCCCCEEEECCCCCEEEEEEECCCCCHHHHCCCCCCCC
YIRARAVLITRADRSDIKSFADLKGKKAAQSLSSNFARLAEQSGAELVGTDGFDQSVQLV
HHHEEEEEEEECCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCEEEECCCCCCEEEEE
LTGRADATVNDSLSFLDFHKQKPNAPVKIAAEQADADASGIIMRKNNPALKAAIDKALEA
EECCCCCCCCCCHHHHHHHCCCCCCCEEEEECCCCCCCCEEEEECCCCHHHHHHHHHHHH
IRADGSYAAIAQKYFGADVSK
HHCCCCHHHHHHHHHCCCCCC
>Mature Secondary Structure
MRFFSASRVAAFVVAALLGAAASAPAKADDLDAIRAAKTLRIGTEGTYAPFTFHDASGQL
CCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHEEECCCCCCCCEEEECCCCCE
VGFDVEIGRKVAEQLGVTPQFLEGKWDGLIAGLDGKRYDVVINQVGITKERQAKFDFSEP
EEEEHHHHHHHHHHHCCCHHHHCCCCCEEEECCCCCEEEEEEECCCCCHHHHCCCCCCCC
YIRARAVLITRADRSDIKSFADLKGKKAAQSLSSNFARLAEQSGAELVGTDGFDQSVQLV
HHHEEEEEEEECCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCEEEECCCCCCEEEEE
LTGRADATVNDSLSFLDFHKQKPNAPVKIAAEQADADASGIIMRKNNPALKAAIDKALEA
EECCCCCCCCCCHHHHHHHCCCCCCCEEEEECCCCCCCCEEEEECCCCHHHHHHHHHHHH
IRADGSYAAIAQKYFGADVSK
HHCCCCHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800; 10675023 [H]