BacMap

Definition	Methylobacterium radiotolerans JCM 2831 chromosome, complete genome.
Accession	NC_010505
Length	6,077,833

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The map label for this gene is ppi [H]

Identifier: 170748567

GI number: 170748567

Start: 2289798

End: 2290346

Strand: Reverse

Name: ppi [H]

Synonym: Mrad2831_2149

Alternate gene names: 170748567

Gene position: 2290346-2289798 (Counterclockwise)

Preceding gene: 170748568

Following gene: 170748566

Centisome position: 37.68

GC content: 68.67

Gene sequence:

>549_bases
ATGAACCGACGCCACGCCCTCCTCGCGCTCGCCCTCACGCTCGGCACCGTCCAGGCCGCCCGGGCCGCCGACGCCAACAC
GGTCTATCTCCAGACCAAGGACGGGCGGATCACCATCGAGCTGCGGCCCGACCTCGCGCCCAAGCACGTCAAGCAGATCA
AGACGCTGGTCTCGCAGGGCTTCTACAACGGCCTCAAGTTCCACCGCGTCATCGACGGCTTCATGGCCCAGACCGGGGAC
CCGAAGGGCAACGGCACCGGCGGCTCCAGCCTGCCGAACATCCCGGCCGAGTTCACGCAGGCGGCCCAGTTCCGCCGCGG
CACCGTGGGCATGGCCCGGTCGCAGGATCCGAACTCGGCCAACTCGCAGTTCTTCATCTGCCTCGGCGACGCGCCGTTCC
TGAACGGCCAGTACACGATCGTCGGCAACGTGACGGACGGGCTCGACGTCCTCGACAAGATCAAGAAGGCCGCCCCCGGC
GCGCCGGGCGGGGCCGTGCAGGACCCCGACAAGATCGTCAGCATGACGCTCGCCGAGAAGGCCAAGTAG

Upstream 100 bases:

>100_bases
GGGCGACGTCTCGCCCTTCGTGCCGGCGGCCGTCGCCGCGCGCCTCCGGCAGCGCTTCGCCGCCCATTCCTGAGACCGGC
CCGACCCTGGAAGGACCACG

Downstream 100 bases:

>100_bases
CGGCCGCATGCCCGGCCGGCGCGCCGCCTCGATCCTCCTGCTGAGCCTGATCCCGGCGGCCGCCGCGGCCTATGACGGCG
CGCCGTTCTACGGCTACGCC

Product: cyclophilin type peptidyl-prolyl cis-trans isomerase

Products: NA

Alternate protein names: PPIase; Rotamase [H]

Number of amino acids: Translated: 182; Mature: 182

Protein sequence:

>182_residues
MNRRHALLALALTLGTVQAARAADANTVYLQTKDGRITIELRPDLAPKHVKQIKTLVSQGFYNGLKFHRVIDGFMAQTGD
PKGNGTGGSSLPNIPAEFTQAAQFRRGTVGMARSQDPNSANSQFFICLGDAPFLNGQYTIVGNVTDGLDVLDKIKKAAPG
APGGAVQDPDKIVSMTLAEKAK

Sequences:

>Translated_182_residues
MNRRHALLALALTLGTVQAARAADANTVYLQTKDGRITIELRPDLAPKHVKQIKTLVSQGFYNGLKFHRVIDGFMAQTGD
PKGNGTGGSSLPNIPAEFTQAAQFRRGTVGMARSQDPNSANSQFFICLGDAPFLNGQYTIVGNVTDGLDVLDKIKKAAPG
APGGAVQDPDKIVSMTLAEKAK
>Mature_182_residues
MNRRHALLALALTLGTVQAARAADANTVYLQTKDGRITIELRPDLAPKHVKQIKTLVSQGFYNGLKFHRVIDGFMAQTGD
PKGNGTGGSSLPNIPAEFTQAAQFRRGTVGMARSQDPNSANSQFFICLGDAPFLNGQYTIVGNVTDGLDVLDKIKKAAPG
APGGAVQDPDKIVSMTLAEKAK

Specific function: PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides [H]

COG id: COG0652

COG function: function code O; Peptidyl-prolyl cis-trans isomerase (rotamase) - cyclophilin family

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PPIase cyclophilin-type domain [H]

Homologues:

Organism=Homo sapiens, GI19557636, Length=133, Percent_Identity=42.1052631578947, Blast_Score=102, Evalue=2e-22,
Organism=Homo sapiens, GI7706339, Length=130, Percent_Identity=39.2307692307692, Blast_Score=92, Evalue=3e-19,
Organism=Homo sapiens, GI24308049, Length=146, Percent_Identity=36.986301369863, Blast_Score=89, Evalue=2e-18,
Organism=Homo sapiens, GI64276486, Length=121, Percent_Identity=42.9752066115703, Blast_Score=86, Evalue=1e-17,
Organism=Homo sapiens, GI5031987, Length=127, Percent_Identity=39.3700787401575, Blast_Score=86, Evalue=2e-17,
Organism=Homo sapiens, GI45439316, Length=154, Percent_Identity=35.7142857142857, Blast_Score=76, Evalue=2e-14,
Organism=Homo sapiens, GI4505991, Length=130, Percent_Identity=39.2307692307692, Blast_Score=75, Evalue=2e-14,
Organism=Homo sapiens, GI10863927, Length=131, Percent_Identity=35.8778625954198, Blast_Score=75, Evalue=2e-14,
Organism=Homo sapiens, GI303305009, Length=150, Percent_Identity=36, Blast_Score=74, Evalue=7e-14,
Organism=Homo sapiens, GI5174637, Length=148, Percent_Identity=35.8108108108108, Blast_Score=74, Evalue=9e-14,
Organism=Homo sapiens, GI150418007, Length=139, Percent_Identity=36.6906474820144, Blast_Score=70, Evalue=1e-12,
Organism=Homo sapiens, GI22547212, Length=131, Percent_Identity=40.4580152671756, Blast_Score=69, Evalue=2e-12,
Organism=Homo sapiens, GI7657473, Length=131, Percent_Identity=40.4580152671756, Blast_Score=69, Evalue=2e-12,
Organism=Homo sapiens, GI22547215, Length=131, Percent_Identity=40.4580152671756, Blast_Score=68, Evalue=4e-12,
Organism=Homo sapiens, GI256017253, Length=126, Percent_Identity=35.7142857142857, Blast_Score=68, Evalue=4e-12,
Organism=Homo sapiens, GI221139811, Length=126, Percent_Identity=35.7142857142857, Blast_Score=68, Evalue=4e-12,
Organism=Homo sapiens, GI256017251, Length=126, Percent_Identity=35.7142857142857, Blast_Score=68, Evalue=4e-12,
Organism=Homo sapiens, GI4758950, Length=128, Percent_Identity=36.71875, Blast_Score=68, Evalue=5e-12,
Organism=Homo sapiens, GI5454154, Length=137, Percent_Identity=34.3065693430657, Blast_Score=67, Evalue=7e-12,
Organism=Homo sapiens, GI20911035, Length=141, Percent_Identity=32.6241134751773, Blast_Score=67, Evalue=9e-12,
Organism=Homo sapiens, GI169163387, Length=97, Percent_Identity=38.1443298969072, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI169164185, Length=97, Percent_Identity=38.1443298969072, Blast_Score=66, Evalue=1e-11,
Organism=Homo sapiens, GI169163623, Length=97, Percent_Identity=38.1443298969072, Blast_Score=66, Evalue=1e-11,
Organism=Homo sapiens, GI310129267, Length=127, Percent_Identity=32.2834645669291, Blast_Score=65, Evalue=4e-11,
Organism=Homo sapiens, GI310120620, Length=127, Percent_Identity=32.2834645669291, Blast_Score=65, Evalue=4e-11,
Organism=Escherichia coli, GI1786736, Length=163, Percent_Identity=38.0368098159509, Blast_Score=95, Evalue=2e-21,
Organism=Escherichia coli, GI1789763, Length=134, Percent_Identity=41.7910447761194, Blast_Score=89, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17506311, Length=151, Percent_Identity=39.0728476821192, Blast_Score=99, Evalue=9e-22,
Organism=Caenorhabditis elegans, GI71980594, Length=132, Percent_Identity=43.1818181818182, Blast_Score=97, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI71980590, Length=132, Percent_Identity=43.1818181818182, Blast_Score=97, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI17532641, Length=151, Percent_Identity=37.0860927152318, Blast_Score=86, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17565860, Length=151, Percent_Identity=38.4105960264901, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17539498, Length=154, Percent_Identity=37.6623376623377, Blast_Score=85, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI71997782, Length=124, Percent_Identity=42.741935483871, Blast_Score=83, Evalue=9e-17,
Organism=Caenorhabditis elegans, GI17539496, Length=129, Percent_Identity=37.984496124031, Blast_Score=82, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17566266, Length=134, Percent_Identity=35.8208955223881, Blast_Score=80, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI17559074, Length=134, Percent_Identity=35.0746268656716, Blast_Score=77, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17552782, Length=151, Percent_Identity=34.4370860927152, Blast_Score=75, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17552780, Length=179, Percent_Identity=29.608938547486, Blast_Score=73, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI32563659, Length=140, Percent_Identity=32.1428571428571, Blast_Score=67, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17506307, Length=128, Percent_Identity=35.15625, Blast_Score=65, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6320359, Length=139, Percent_Identity=38.8489208633094, Blast_Score=84, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6323562, Length=139, Percent_Identity=35.9712230215827, Blast_Score=74, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6321848, Length=141, Percent_Identity=37.5886524822695, Blast_Score=69, Evalue=3e-13,
Organism=Drosophila melanogaster, GI17986117, Length=139, Percent_Identity=38.1294964028777, Blast_Score=89, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24652460, Length=133, Percent_Identity=39.0977443609023, Blast_Score=87, Evalue=9e-18,
Organism=Drosophila melanogaster, GI19922376, Length=142, Percent_Identity=35.9154929577465, Blast_Score=84, Evalue=4e-17,
Organism=Drosophila melanogaster, GI45549139, Length=127, Percent_Identity=40.1574803149606, Blast_Score=84, Evalue=4e-17,
Organism=Drosophila melanogaster, GI21357249, Length=122, Percent_Identity=40.9836065573771, Blast_Score=84, Evalue=6e-17,
Organism=Drosophila melanogaster, GI24664125, Length=129, Percent_Identity=37.984496124031, Blast_Score=83, Evalue=1e-16,
Organism=Drosophila melanogaster, GI21357783, Length=129, Percent_Identity=37.984496124031, Blast_Score=83, Evalue=1e-16,
Organism=Drosophila melanogaster, GI17647301, Length=129, Percent_Identity=37.2093023255814, Blast_Score=76, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24661775, Length=146, Percent_Identity=33.5616438356164, Blast_Score=74, Evalue=4e-14,
Organism=Drosophila melanogaster, GI21355237, Length=146, Percent_Identity=33.5616438356164, Blast_Score=74, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24661771, Length=146, Percent_Identity=33.5616438356164, Blast_Score=74, Evalue=4e-14,
Organism=Drosophila melanogaster, GI20130249, Length=141, Percent_Identity=36.8794326241135, Blast_Score=70, Evalue=6e-13,
Organism=Drosophila melanogaster, GI17136360, Length=164, Percent_Identity=33.5365853658537, Blast_Score=69, Evalue=1e-12,
Organism=Drosophila melanogaster, GI19922912, Length=154, Percent_Identity=38.3116883116883, Blast_Score=68, Evalue=3e-12,
Organism=Drosophila melanogaster, GI21355677, Length=141, Percent_Identity=34.0425531914894, Blast_Score=65, Evalue=2e-11,

Paralogues:

None

Copy number: 780 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015891
- InterPro:   IPR020892
- InterPro:   IPR002130 [H]

Pfam domain/function: PF00160 Pro_isomerase [H]

EC number: =5.2.1.8 [H]

Molecular weight: Translated: 19335; Mature: 19335

Theoretical pI: Translated: 10.19; Mature: 10.19

Prosite motif: PS00170 CSA_PPIASE_1 ; PS50072 CSA_PPIASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRRHALLALALTLGTVQAARAADANTVYLQTKDGRITIELRPDLAPKHVKQIKTLVSQG
CCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCEEEEEECCCCCHHHHHHHHHHHHHH
FYNGLKFHRVIDGFMAQTGDPKGNGTGGSSLPNIPAEFTQAAQFRRGTVGMARSQDPNSA
HHCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCC
NSQFFICLGDAPFLNGQYTIVGNVTDGLDVLDKIKKAAPGAPGGAVQDPDKIVSMTLAEK
CCEEEEEECCCCEECCEEEEEECCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHC
AK
CC
>Mature Secondary Structure
MNRRHALLALALTLGTVQAARAADANTVYLQTKDGRITIELRPDLAPKHVKQIKTLVSQG
CCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCEEEEEECCCCCHHHHHHHHHHHHHH
FYNGLKFHRVIDGFMAQTGDPKGNGTGGSSLPNIPAEFTQAAQFRRGTVGMARSQDPNSA
HHCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCC
NSQFFICLGDAPFLNGQYTIVGNVTDGLDVLDKIKKAAPGAPGGAVQDPDKIVSMTLAEK
CCEEEEEECCCCEECCEEEEEECCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHC
AK
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA