Click here to switch to the map view.

The map label for this gene is dctA4 [H]

Identifier: 170748547

GI number: 170748547

Start: 2269027

End: 2270400

Strand: Reverse

Name: dctA4 [H]

Synonym: Mrad2831_2129

Alternate gene names: 170748547

Gene position: 2270400-2269027 (Counterclockwise)

Preceding gene: 170748548

Following gene: 170748546

Centisome position: 37.36

GC content: 67.32

Gene sequence:

>1374_bases
ATGGCACAGGCAATCCAGGCGACGGCTCCCGCCGCAGCGCCCCGCGAGCCCTGGTACAAGGTGCTCTACATCCAGGTGCT
GATCGCCATCGCGCTCGGCGTGCTGCTCGGCTGGTACGACCCGGCCACCGGCAAGTCGATGAAGTGGCTCGGCGACGCCT
TCATCGGCCTGATCAAGATGATGATCGCGCCGATCATCTTCTGCACGATCGTCCACGGCATCGCGTCGATCGGCGACCTG
AAGAAGGTCGGCCGCGTCGGCCTGAAGGCGCTGGTCTACTTCGAGGTCGTGTCCACCATCGCGCTGCTGATCGGCGTCAT
CGTCGGTGAGGTGATCCGGCCCGGCGCCGGCTTCAACGCCGACCCGTCGAAGCTCGATGCCAGCAAGGTCGCGACCTACG
CCAGCAAGGCGGCGGCCGATTCCACGGTCGCCCACATCCTGGCGATCATCCCGAAGAGCTTCCTCGACCCCTTCGCCACC
GGCGACCTGCTGCAGGTGCTGCTGATCTCGATCCTCACCGGCGTCGTGGTGACGAATCTCGGCGATCGGGCGGCCTCGGT
GAACCACGCGATCGACACGGCCGGCCAGATCTTCTTCCGGATCATCGGCATCGTCGTGAAGCTCGCCCCCGTCGGCGCCT
TCGGCGCCATGGCGTTCACGATCGGCGAGTACGGGATCCAGAAGGTCTACGACCTCGCGTGGCTCGTCGGCACCTTCTAC
ATCACGGCGCTGCTGTTCGTGCTCGTGGTGCTCGGGGCGATCGCCCGGGTGGCGGGCTTCTCGATCCTGCGCTTCCTCGC
CTACATCAAGGACGAGCTGCTGATCGTGGTCGGCACGTCGTCCTCCGAGACGGTGCTGCCGCAGCTCATGACCAAGATGC
GCCGCCTCGGCGCCTCGGACTCGGTCGTGGGCCTCGTGGTGCCGACCGGCTACTCGTTCAACCTCGACGGCACCAACATC
TACATGACGCTGGCGACGCTGTTCCTCGCGCAGGCCGTGGGTGTGGACCTGTCGATCAGCCAGTACGCGACCATCATCCT
GGTGGCGATGCTGACCTCGAAGGGCGCCTCGGGCGTCACCGGCGCGGGCTTCATCACCCTGGCGGCGACGCTCTCGGCGA
TCCCCAACAGCCCGGTCCCGGTCGCCGCCATGACCCTGGTCCTCGGCATCGACAAGTTCATGTCCGAGTGCCGCGCGCTC
ACCAACCTCGTGGGCAACGGCGTCGCCTGCGTGGTGGTCAGCCGCTGGGAGGGCGAGCTCGACGTCGCCAAGCTCAACGA
GGTGATGGCCCACCCGGTCGCCATCGGCACCCACATCGCCGACGAGGCGCCGCAGCCGATGGGCACCGACAGCAAGGTCG
CCGCCGCCGAGTGA

Upstream 100 bases:

>100_bases
TGGTTCCGGGCCGATAAGCGTGCAGCCGTTGAGGCGCCGGGTGATAATCGCTAGCGTGCCGGCAAATCGGGAGGGTGCGC
GGTCGTCGCACGGGATGGAC

Downstream 100 bases:

>100_bases
TGGGATCGCCCGCGGCGGAGAACACAGGTCGCCCGCGCCTCGGCATCGATCTCGGCGGCACCAAGATCGCCGGGATCGTG
CTCAGCCGGGACGGGACGAC

Product: sodium:dicarboxylate symporter

Products: orotate [Cytoplasm]; fumarate [Cytoplasm]; malate [Cytoplasm]; Na (I) [Cytoplasm]; succinate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 457; Mature: 456

Protein sequence:

>457_residues
MAQAIQATAPAAAPREPWYKVLYIQVLIAIALGVLLGWYDPATGKSMKWLGDAFIGLIKMMIAPIIFCTIVHGIASIGDL
KKVGRVGLKALVYFEVVSTIALLIGVIVGEVIRPGAGFNADPSKLDASKVATYASKAAADSTVAHILAIIPKSFLDPFAT
GDLLQVLLISILTGVVVTNLGDRAASVNHAIDTAGQIFFRIIGIVVKLAPVGAFGAMAFTIGEYGIQKVYDLAWLVGTFY
ITALLFVLVVLGAIARVAGFSILRFLAYIKDELLIVVGTSSSETVLPQLMTKMRRLGASDSVVGLVVPTGYSFNLDGTNI
YMTLATLFLAQAVGVDLSISQYATIILVAMLTSKGASGVTGAGFITLAATLSAIPNSPVPVAAMTLVLGIDKFMSECRAL
TNLVGNGVACVVVSRWEGELDVAKLNEVMAHPVAIGTHIADEAPQPMGTDSKVAAAE

Sequences:

>Translated_457_residues
MAQAIQATAPAAAPREPWYKVLYIQVLIAIALGVLLGWYDPATGKSMKWLGDAFIGLIKMMIAPIIFCTIVHGIASIGDL
KKVGRVGLKALVYFEVVSTIALLIGVIVGEVIRPGAGFNADPSKLDASKVATYASKAAADSTVAHILAIIPKSFLDPFAT
GDLLQVLLISILTGVVVTNLGDRAASVNHAIDTAGQIFFRIIGIVVKLAPVGAFGAMAFTIGEYGIQKVYDLAWLVGTFY
ITALLFVLVVLGAIARVAGFSILRFLAYIKDELLIVVGTSSSETVLPQLMTKMRRLGASDSVVGLVVPTGYSFNLDGTNI
YMTLATLFLAQAVGVDLSISQYATIILVAMLTSKGASGVTGAGFITLAATLSAIPNSPVPVAAMTLVLGIDKFMSECRAL
TNLVGNGVACVVVSRWEGELDVAKLNEVMAHPVAIGTHIADEAPQPMGTDSKVAAAE
>Mature_456_residues
AQAIQATAPAAAPREPWYKVLYIQVLIAIALGVLLGWYDPATGKSMKWLGDAFIGLIKMMIAPIIFCTIVHGIASIGDLK
KVGRVGLKALVYFEVVSTIALLIGVIVGEVIRPGAGFNADPSKLDASKVATYASKAAADSTVAHILAIIPKSFLDPFATG
DLLQVLLISILTGVVVTNLGDRAASVNHAIDTAGQIFFRIIGIVVKLAPVGAFGAMAFTIGEYGIQKVYDLAWLVGTFYI
TALLFVLVVLGAIARVAGFSILRFLAYIKDELLIVVGTSSSETVLPQLMTKMRRLGASDSVVGLVVPTGYSFNLDGTNIY
MTLATLFLAQAVGVDLSISQYATIILVAMLTSKGASGVTGAGFITLAATLSAIPNSPVPVAAMTLVLGIDKFMSECRALT
NLVGNGVACVVVSRWEGELDVAKLNEVMAHPVAIGTHIADEAPQPMGTDSKVAAAE

Specific function: Responsible for the transport of dicarboxylates such as succinate, fumarate, and malate from the periplasm across the membrane [H]

COG id: COG1301

COG function: function code C; Na+/H+-dicarboxylate symporters

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family [H]

Homologues:

Organism=Homo sapiens, GI169790839, Length=482, Percent_Identity=24.2738589211618, Blast_Score=103, Evalue=5e-22,
Organism=Homo sapiens, GI4827012, Length=275, Percent_Identity=27.6363636363636, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI40254478, Length=431, Percent_Identity=25.5220417633411, Blast_Score=98, Evalue=2e-20,
Organism=Homo sapiens, GI21314632, Length=459, Percent_Identity=23.9651416122004, Blast_Score=86, Evalue=8e-17,
Organism=Homo sapiens, GI66773030, Length=430, Percent_Identity=22.7906976744186, Blast_Score=84, Evalue=3e-16,
Organism=Homo sapiens, GI5032093, Length=378, Percent_Identity=25.3968253968254, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI194239697, Length=262, Percent_Identity=25.1908396946565, Blast_Score=72, Evalue=9e-13,
Organism=Homo sapiens, GI262359914, Length=387, Percent_Identity=23.5142118863049, Blast_Score=69, Evalue=8e-12,
Organism=Homo sapiens, GI301601644, Length=166, Percent_Identity=27.710843373494, Blast_Score=68, Evalue=1e-11,
Organism=Homo sapiens, GI223468564, Length=255, Percent_Identity=25.4901960784314, Blast_Score=67, Evalue=4e-11,
Organism=Homo sapiens, GI223468566, Length=255, Percent_Identity=25.4901960784314, Blast_Score=66, Evalue=5e-11,
Organism=Escherichia coli, GI1789947, Length=425, Percent_Identity=54.3529411764706, Blast_Score=462, Evalue=1e-131,
Organism=Escherichia coli, GI1790514, Length=414, Percent_Identity=37.43961352657, Blast_Score=288, Evalue=5e-79,
Organism=Escherichia coli, GI1788024, Length=417, Percent_Identity=23.5011990407674, Blast_Score=86, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17537407, Length=435, Percent_Identity=26.2068965517241, Blast_Score=135, Evalue=4e-32,
Organism=Caenorhabditis elegans, GI71996953, Length=450, Percent_Identity=23.7777777777778, Blast_Score=113, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI71983099, Length=458, Percent_Identity=24.0174672489083, Blast_Score=101, Evalue=6e-22,
Organism=Caenorhabditis elegans, GI71983106, Length=458, Percent_Identity=24.0174672489083, Blast_Score=101, Evalue=7e-22,
Organism=Caenorhabditis elegans, GI193206654, Length=449, Percent_Identity=22.4944320712695, Blast_Score=86, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI193206505, Length=456, Percent_Identity=21.7105263157895, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17541374, Length=410, Percent_Identity=21.9512195121951, Blast_Score=68, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24583025, Length=462, Percent_Identity=21.4285714285714, Blast_Score=97, Evalue=3e-20,
Organism=Drosophila melanogaster, GI17137668, Length=462, Percent_Identity=21.4285714285714, Blast_Score=97, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24583023, Length=462, Percent_Identity=21.4285714285714, Blast_Score=97, Evalue=3e-20,
Organism=Drosophila melanogaster, GI281360481, Length=437, Percent_Identity=25.4004576659039, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI281360483, Length=437, Percent_Identity=25.4004576659039, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI17137666, Length=437, Percent_Identity=25.4004576659039, Blast_Score=82, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001991
- InterPro:   IPR018107 [H]

Pfam domain/function: PF00375 SDF [H]

EC number: NA

Molecular weight: Translated: 47796; Mature: 47665

Theoretical pI: Translated: 7.04; Mature: 7.04

Prosite motif: PS00714 NA_DICARBOXYL_SYMP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
AQAIQATAPAAAPREPWYKVLYIQVLIAIALGVLLGWYDPATGKSMKWLGDAFIGLIKM
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHH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HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: orotate [Periplasm]; fumarate [Periplasm]; malate [Periplasm]; Na (I) [Periplasm]; succinate [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + orotate [Periplasm] = Na (I) [Cytoplasm] + orotate [Cytoplasm] Na (I) [Periplasm] + fumarate [Periplasm] = Na (I) [Cytoplasm] + fumarate [Cytoplasm] Na (I) [Periplasm] + malate [Periplasm] = Na (I) [Cytoplasm] + malate [Cytoplasm] Na

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12597275 [H]