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The map label for this gene is mak [H]

Identifier: 170748546

GI number: 170748546

Start: 2268089

End: 2269027

Strand: Reverse

Name: mak [H]

Synonym: Mrad2831_2128

Alternate gene names: 170748546

Gene position: 2269027-2268089 (Counterclockwise)

Preceding gene: 170748547

Following gene: 170748545

Centisome position: 37.33

GC content: 77.0

Gene sequence:

>939_bases
ATGGGATCGCCCGCGGCGGAGAACACAGGTCGCCCGCGCCTCGGCATCGATCTCGGCGGCACCAAGATCGCCGGGATCGT
GCTCAGCCGGGACGGGACGACCCTGGCCGAGGCGCGGATGCCCGCCCCGCGCGGCGCCTACCGGGCCACCGTCGAGGCCG
TGGCCGACCTCGTCCTGCGTCTCGAGGCCGAGGCCGGCGCGCCCTGCAGCGTCGGCATCGGCATGCCGGGGAGCCTGTCC
CCGGCGACGGGCCTCGTGCGCAACGCCAACTCGCACTGGCTCAACGGACACCCGTTCGCGGCCGATCTCGGCGCGCGCCT
GGAGCGCCCGCTGCGGATCGAGAATGACGCCAATTGCCTGGCGGTGTCGGAGGCGATCGACGGCGCCGGGGCCGGGGCCA
GCGTGGTCTGGGCGGTGATCCTGGGGACCGGCGTCGGGTCCGGCATCGCCCTCGACGGTCGGGTGCTCACGGGCCGCAAC
GGCATCGCGGGTGAGTGGGGCCACGGCCCGCTGCCGGCGCCCCGCGACGACGAGCGGCCGGGCGCCGCCTGCTACTGCGG
TCGCCGGGGCTGCGTCGAGACCTGGCTGTCCGGTCCCGGCCTGGCGGCGGACCACGCGCGGCGGCACGGCGGCAGCCTCG
CCGCCGAGGCGATCGTCACCGCGGCCCGGGCCGGAAGCCCGGCGGCGCGGGAGACGCTCGCCGTCCATCTCGAGCGGCTC
GGGCGTGCGGCCGCCCAGGTGGTGAACCTCCTCGACCCGGACGTGATCGTGATCGGCGGCGGCCTGTCGCGCATCCCGGA
GCTGATCGCGGGCCTGCCGGCGGCGATCGCCCCCCACGTCTTCTCCGATGCCTTCGACACGCCCGTGCGGGCGAGCCTGC
ACGGCGACGCCTCGGGCGTGCGCGGGGCGGCGTGGCTGTGGGAGGCGGAGGCCGGCTGA

Upstream 100 bases:

>100_bases
TCAACGAGGTGATGGCCCACCCGGTCGCCATCGGCACCCACATCGCCGACGAGGCGCCGCAGCCGATGGGCACCGACAGC
AAGGTCGCCGCCGCCGAGTG

Downstream 100 bases:

>100_bases
CAGCGGCCCAAGGCTTGCCCAAGGCTTGCCCAAGGCTTGCCCGACGCTTGCCCGAGCCCGATGGAACTGCCGAGCTCACG
GAGACGTCGCTTGCGGGTCC

Product: ROK family protein

Products: NA

Alternate protein names: D-fructose kinase; Manno(fructo)kinase [H]

Number of amino acids: Translated: 312; Mature: 311

Protein sequence:

>312_residues
MGSPAAENTGRPRLGIDLGGTKIAGIVLSRDGTTLAEARMPAPRGAYRATVEAVADLVLRLEAEAGAPCSVGIGMPGSLS
PATGLVRNANSHWLNGHPFAADLGARLERPLRIENDANCLAVSEAIDGAGAGASVVWAVILGTGVGSGIALDGRVLTGRN
GIAGEWGHGPLPAPRDDERPGAACYCGRRGCVETWLSGPGLAADHARRHGGSLAAEAIVTAARAGSPAARETLAVHLERL
GRAAAQVVNLLDPDVIVIGGGLSRIPELIAGLPAAIAPHVFSDAFDTPVRASLHGDASGVRGAAWLWEAEAG

Sequences:

>Translated_312_residues
MGSPAAENTGRPRLGIDLGGTKIAGIVLSRDGTTLAEARMPAPRGAYRATVEAVADLVLRLEAEAGAPCSVGIGMPGSLS
PATGLVRNANSHWLNGHPFAADLGARLERPLRIENDANCLAVSEAIDGAGAGASVVWAVILGTGVGSGIALDGRVLTGRN
GIAGEWGHGPLPAPRDDERPGAACYCGRRGCVETWLSGPGLAADHARRHGGSLAAEAIVTAARAGSPAARETLAVHLERL
GRAAAQVVNLLDPDVIVIGGGLSRIPELIAGLPAAIAPHVFSDAFDTPVRASLHGDASGVRGAAWLWEAEAG
>Mature_311_residues
GSPAAENTGRPRLGIDLGGTKIAGIVLSRDGTTLAEARMPAPRGAYRATVEAVADLVLRLEAEAGAPCSVGIGMPGSLSP
ATGLVRNANSHWLNGHPFAADLGARLERPLRIENDANCLAVSEAIDGAGAGASVVWAVILGTGVGSGIALDGRVLTGRNG
IAGEWGHGPLPAPRDDERPGAACYCGRRGCVETWLSGPGLAADHARRHGGSLAAEAIVTAARAGSPAARETLAVHLERLG
RAAAQVVNLLDPDVIVIGGGLSRIPELIAGLPAAIAPHVFSDAFDTPVRASLHGDASGVRGAAWLWEAEAG

Specific function: Catalyzes the phosphorylation of fructose to fructose-6- P. Has also low level glucokinase activity in vitro. Is not able to phosphorylate D-ribose, D-mannitol, D-sorbitol, inositol, and L-threonine [H]

COG id: COG1940

COG function: function code KG; Transcriptional regulator/sugar kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ROK (nagC/xylR) family [H]

Homologues:

Organism=Homo sapiens, GI298566325, Length=275, Percent_Identity=27.2727272727273, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI4885285, Length=275, Percent_Identity=27.2727272727273, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI190014632, Length=275, Percent_Identity=27.2727272727273, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI298566315, Length=275, Percent_Identity=27.2727272727273, Blast_Score=70, Evalue=2e-12,
Organism=Escherichia coli, GI87081733, Length=301, Percent_Identity=52.1594684385382, Blast_Score=308, Evalue=2e-85,
Organism=Escherichia coli, GI1787363, Length=296, Percent_Identity=38.5135135135135, Blast_Score=192, Evalue=2e-50,
Organism=Escherichia coli, GI1787878, Length=237, Percent_Identity=27.0042194092827, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1786891, Length=285, Percent_Identity=24.2105263157895, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1790522, Length=256, Percent_Identity=26.953125, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI87082230, Length=276, Percent_Identity=31.8840579710145, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000600 [H]

Pfam domain/function: PF00480 ROK [H]

EC number: =2.7.1.4 [H]

Molecular weight: Translated: 31544; Mature: 31413

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: PS01125 ROK ; PS01036 HSP70_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGSPAAENTGRPRLGIDLGGTKIAGIVLSRDGTTLAEARMPAPRGAYRATVEAVADLVLR
CCCCCCCCCCCCEEEEEECCCEEEEEEEECCCCCHHHHCCCCCCCHHHHHHHHHHHHHHH
LEAEAGAPCSVGIGMPGSLSPATGLVRNANSHWLNGHPFAADLGARLERPLRIENDANCL
HCCCCCCCEEECCCCCCCCCHHHHHHCCCCCCCCCCCCCHHHHHHHCCCCEEECCCCCEE
AVSEAIDGAGAGASVVWAVILGTGVGSGIALDGRVLTGRNGIAGEWGHGPLPAPRDDERP
EEHHHHCCCCCCHHHHHHHHHHCCCCCCEEECCEEEECCCCCCCCCCCCCCCCCCCCCCC
GAACYCGRRGCVETWLSGPGLAADHARRHGGSLAAEAIVTAARAGSPAARETLAVHLERL
CCEEECCCCHHHHHHHCCCCCCHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
GRAAAQVVNLLDPDVIVIGGGLSRIPELIAGLPAAIAPHVFSDAFDTPVRASLHGDASGV
HHHHHHHHHHCCCCEEEECCCHHHHHHHHHCCCHHHHHHHHHHHCCCCCEEECCCCCCCC
RGAAWLWEAEAG
CCEEEEEECCCC
>Mature Secondary Structure 
GSPAAENTGRPRLGIDLGGTKIAGIVLSRDGTTLAEARMPAPRGAYRATVEAVADLVLR
CCCCCCCCCCCEEEEEECCCEEEEEEEECCCCCHHHHCCCCCCCHHHHHHHHHHHHHHH
LEAEAGAPCSVGIGMPGSLSPATGLVRNANSHWLNGHPFAADLGARLERPLRIENDANCL
HCCCCCCCEEECCCCCCCCCHHHHHHCCCCCCCCCCCCCHHHHHHHCCCCEEECCCCCEE
AVSEAIDGAGAGASVVWAVILGTGVGSGIALDGRVLTGRNGIAGEWGHGPLPAPRDDERP
EEHHHHCCCCCCHHHHHHHHHHCCCCCCEEECCEEEECCCCCCCCCCCCCCCCCCCCCCC
GAACYCGRRGCVETWLSGPGLAADHARRHGGSLAAEAIVTAARAGSPAARETLAVHLERL
CCEEECCCCHHHHHHHCCCCCCHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
GRAAAQVVNLLDPDVIVIGGGLSRIPELIAGLPAAIAPHVFSDAFDTPVRASLHGDASGV
HHHHHHHHHHCCCCEEEECCCHHHHHHHHHCCCHHHHHHHHHHHCCCCCEEECCCCCCCC
RGAAWLWEAEAG
CCEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8807285; 9278503; 1744033 [H]