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The map label for this gene is ilvG [H]

Identifier: 170748535

GI number: 170748535

Start: 2252523

End: 2254193

Strand: Reverse

Name: ilvG [H]

Synonym: Mrad2831_2117

Alternate gene names: 170748535

Gene position: 2254193-2252523 (Counterclockwise)

Preceding gene: 170748536

Following gene: 170748534

Centisome position: 37.09

GC content: 74.69

Gene sequence:

>1671_bases
TTGAGCGGATTGCCCCCACGCACCGGCGCGCAGGTGCTCGTCGATCAGCTCCGAGCGCAGGGTGTCGAGCGCGTGACCTG
CGTCCCCGGCGAGAGCTACCTCGCGGTGCTCGACGCCCTGCGCGACAGCGGTATCGACGTGCTGGTCTGCCGGCAGGAGG
GCGGCGCGGCGATCATGGCGGAGGCCGCCGGCAAGCTCACGGGCCGCCCGGGCATCTGCTTCGTCACCCGGGGGCCCGGC
GCCACCAACGCCTCCGCCGGCGTGCACATCGCCCGCCAGGATTCGACGCCGATGATCCTGTTCGTCGGCCAGATCGCCCG
GCGGATGCGGGATCGCGAGGCGTTCCAGGAGGTCGATTACCGGCAGATGTTCGGCGGCGTCGCCAAGTGGGCGGTGGAGA
TCGACGACGCCGCGCGCATCCCGGAGATCCTCGCCCGGGCCTTCCGGGTCGCGATGCAGGGCCGCCCGGGCCCCGTCGTG
GTCGCCCTGCCGGAGGACATGCTCGACGACGTCGCGGCCGTGCCGGACGCGCCGCGCGTCGTTCCGGCCGCGCCGGCCCC
GTCCGGGGATGACGTCGCGCGCCTGCGGGCGATGCTGGCGGAGGCGCGGCGGCCGGTGGTGCTGCTGGGCGGATCCCGCT
GGACGCCGGGCGCCGTCGCCGCCATGGCCGCCTGGGCCGAGCGCCACGACCTGCCGGTGGCGGTCTCGTTCCGGCGGGCT
CAGCTCTTCCCCGCCGACCACCCGAACTTCGCGGGCGAGCTCGGGATCGGGCCGAATCCGGCGCTGCTCGCCCGGATCCG
GGAGGCCGACCTGCTGCTGATGGTGGGCGGCCGCCTCTCCGAGATGCCCGCGCAATCCTACGGCCTGCTCGGCATCCCCG
ATCCCGGCCTGCCGCTGGTCCACGTCCACGCCGACGCGGCGGAGCTCGGCCGGGTCTACCAGCCGGCGCTGGCGATCGAG
GCGGCGCCCGAGACCTTCTGCGCGGTCCTGCCGGATTTCGAGGCCACCGGGACCGGCCGCGCCGCCGAGGCCCACGCCGC
CTACCGCGCGTGGTCCGAGACGCCGGAGCCGAAGCCCGGCGCGTTCCAGTACGGCGCGGCGATCACCTGGCTGCGCGAGC
GGCTGCCGCCGGACGCGGTGATCTGCAACGGCGCCGGCAACTTCGCCACCTGGGTCCACCGCTACTACCGGTTCCGCGCC
TTCGGCACGCAGCTCGCGCCGACCTCCGGCTCCATGGGCTACGGGCTGCCGGCCGCCGTGATGGCCAAGCGCAGCCGCCC
GGAGCGGATCGTCGTGGCGCTGGCCGGCGACGGCGACGTGATGATGACCGTGCAGGAATTCGCCACCGCCGTGCAGTACG
GGATCGCCGTCGTGGTGGTCGTGCTCGACAACGGCATGTACGGCACGATCCGGATGCACCAAGAGCGCGAGTATCCCGGC
CGCGTCTCGGCGACGGAGCTGCGCAACCCCGACTTCGCGGCCCTCGCCCGCGCCTGCGGCGGCCACGGCGAGCGCGTGGA
GCGCACCGAGGAGTTCGCCCCCGCCTTCGAGCGCGGCCTCGCCTCCGGGCTGCCGGCGATGATCCACTGCCTCGTCGATC
CCGAGGCGCTCACCCCGACCATGACCCTCGCGGCTATCCGCGAGAAGGCCCTGGCGGCGCAGCGGGGCTGA

Upstream 100 bases:

>100_bases
CCTGAAACTGGGATATCAGACGCAGCTCAGCGGCGGGCGGATCCACGGCCGATCCCGGCACCACGCCCCGGATGCGGCCC
GAGCGCCAGGGAGGAACGGC

Downstream 100 bases:

>100_bases
CCGCCCGCACGCGCCGTCCCGGTGCGGCCCCGCCCGCACACCCTATCGAGAGGAGCAGCTCATGACCCTGCGCGGCGAGA
ACCTGATCGGCGCCGAGGCG

Product: thiamine pyrophosphate protein

Products: NA

Alternate protein names: AHAS-II; ALS-II; Acetohydroxy-acid synthase II large subunit [H]

Number of amino acids: Translated: 556; Mature: 555

Protein sequence:

>556_residues
MSGLPPRTGAQVLVDQLRAQGVERVTCVPGESYLAVLDALRDSGIDVLVCRQEGGAAIMAEAAGKLTGRPGICFVTRGPG
ATNASAGVHIARQDSTPMILFVGQIARRMRDREAFQEVDYRQMFGGVAKWAVEIDDAARIPEILARAFRVAMQGRPGPVV
VALPEDMLDDVAAVPDAPRVVPAAPAPSGDDVARLRAMLAEARRPVVLLGGSRWTPGAVAAMAAWAERHDLPVAVSFRRA
QLFPADHPNFAGELGIGPNPALLARIREADLLLMVGGRLSEMPAQSYGLLGIPDPGLPLVHVHADAAELGRVYQPALAIE
AAPETFCAVLPDFEATGTGRAAEAHAAYRAWSETPEPKPGAFQYGAAITWLRERLPPDAVICNGAGNFATWVHRYYRFRA
FGTQLAPTSGSMGYGLPAAVMAKRSRPERIVVALAGDGDVMMTVQEFATAVQYGIAVVVVVLDNGMYGTIRMHQEREYPG
RVSATELRNPDFAALARACGGHGERVERTEEFAPAFERGLASGLPAMIHCLVDPEALTPTMTLAAIREKALAAQRG

Sequences:

>Translated_556_residues
MSGLPPRTGAQVLVDQLRAQGVERVTCVPGESYLAVLDALRDSGIDVLVCRQEGGAAIMAEAAGKLTGRPGICFVTRGPG
ATNASAGVHIARQDSTPMILFVGQIARRMRDREAFQEVDYRQMFGGVAKWAVEIDDAARIPEILARAFRVAMQGRPGPVV
VALPEDMLDDVAAVPDAPRVVPAAPAPSGDDVARLRAMLAEARRPVVLLGGSRWTPGAVAAMAAWAERHDLPVAVSFRRA
QLFPADHPNFAGELGIGPNPALLARIREADLLLMVGGRLSEMPAQSYGLLGIPDPGLPLVHVHADAAELGRVYQPALAIE
AAPETFCAVLPDFEATGTGRAAEAHAAYRAWSETPEPKPGAFQYGAAITWLRERLPPDAVICNGAGNFATWVHRYYRFRA
FGTQLAPTSGSMGYGLPAAVMAKRSRPERIVVALAGDGDVMMTVQEFATAVQYGIAVVVVVLDNGMYGTIRMHQEREYPG
RVSATELRNPDFAALARACGGHGERVERTEEFAPAFERGLASGLPAMIHCLVDPEALTPTMTLAAIREKALAAQRG
>Mature_555_residues
SGLPPRTGAQVLVDQLRAQGVERVTCVPGESYLAVLDALRDSGIDVLVCRQEGGAAIMAEAAGKLTGRPGICFVTRGPGA
TNASAGVHIARQDSTPMILFVGQIARRMRDREAFQEVDYRQMFGGVAKWAVEIDDAARIPEILARAFRVAMQGRPGPVVV
ALPEDMLDDVAAVPDAPRVVPAAPAPSGDDVARLRAMLAEARRPVVLLGGSRWTPGAVAAMAAWAERHDLPVAVSFRRAQ
LFPADHPNFAGELGIGPNPALLARIREADLLLMVGGRLSEMPAQSYGLLGIPDPGLPLVHVHADAAELGRVYQPALAIEA
APETFCAVLPDFEATGTGRAAEAHAAYRAWSETPEPKPGAFQYGAAITWLRERLPPDAVICNGAGNFATWVHRYYRFRAF
GTQLAPTSGSMGYGLPAAVMAKRSRPERIVVALAGDGDVMMTVQEFATAVQYGIAVVVVVLDNGMYGTIRMHQEREYPGR
VSATELRNPDFAALARACGGHGERVERTEEFAPAFERGLASGLPAMIHCLVDPEALTPTMTLAAIREKALAAQRG

Specific function: Catalyzes the first step in the biosynthesis of branched-chain amino acids [H]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=559, Percent_Identity=23.613595706619, Blast_Score=138, Evalue=1e-32,
Organism=Homo sapiens, GI21361361, Length=561, Percent_Identity=25.8467023172906, Blast_Score=104, Evalue=2e-22,
Organism=Escherichia coli, GI1790104, Length=539, Percent_Identity=31.1688311688312, Blast_Score=223, Evalue=2e-59,
Organism=Escherichia coli, GI87081685, Length=539, Percent_Identity=30.2411873840445, Blast_Score=221, Evalue=8e-59,
Organism=Escherichia coli, GI1786717, Length=536, Percent_Identity=26.6791044776119, Blast_Score=153, Evalue=2e-38,
Organism=Escherichia coli, GI1787096, Length=547, Percent_Identity=25.5941499085923, Blast_Score=126, Evalue=3e-30,
Organism=Escherichia coli, GI1788716, Length=579, Percent_Identity=25.5613126079447, Blast_Score=99, Evalue=9e-22,
Organism=Caenorhabditis elegans, GI17531299, Length=562, Percent_Identity=24.5551601423488, Blast_Score=112, Evalue=6e-25,
Organism=Caenorhabditis elegans, GI17531301, Length=562, Percent_Identity=24.5551601423488, Blast_Score=112, Evalue=7e-25,
Organism=Caenorhabditis elegans, GI17542570, Length=563, Percent_Identity=21.8472468916519, Blast_Score=96, Evalue=6e-20,
Organism=Saccharomyces cerevisiae, GI6323755, Length=563, Percent_Identity=26.9982238010657, Blast_Score=200, Evalue=5e-52,
Organism=Drosophila melanogaster, GI19922626, Length=491, Percent_Identity=25.8655804480652, Blast_Score=122, Evalue=7e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 59237; Mature: 59106

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGLPPRTGAQVLVDQLRAQGVERVTCVPGESYLAVLDALRDSGIDVLVCRQEGGAAIMA
CCCCCCCCCHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHCCCEEEEEECCCCEEEEE
EAAGKLTGRPGICFVTRGPGATNASAGVHIARQDSTPMILFVGQIARRMRDREAFQEVDY
HHCCCCCCCCCEEEEECCCCCCCCCCCEEEEECCCCCEEEEHHHHHHHHHHHHHHHHCCH
RQMFGGVAKWAVEIDDAARIPEILARAFRVAMQGRPGPVVVALPEDMLDDVAAVPDAPRV
HHHHCCHHEEEEEECCHHHHHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHCCCCCCE
VPAAPAPSGDDVARLRAMLAEARRPVVLLGGSRWTPGAVAAMAAWAERHDLPVAVSFRRA
EECCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHCCCCEEEEEEHE
QLFPADHPNFAGELGIGPNPALLARIREADLLLMVGGRLSEMPAQSYGLLGIPDPGLPLV
EECCCCCCCCCCCCCCCCCHHHHHHHHCCCEEEEECCCHHHCCHHHCCEEECCCCCCCEE
HVHADAAELGRVYQPALAIEAAPETFCAVLPDFEATGTGRAAEAHAAYRAWSETPEPKPG
EEECCHHHHHHHHCCHHEEECCCHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCC
AFQYGAAITWLRERLPPDAVICNGAGNFATWVHRYYRFRAFGTQLAPTSGSMGYGLPAAV
CCHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHCCEECCCCCCCCCCCHHHH
MAKRSRPERIVVALAGDGDVMMTVQEFATAVQYGIAVVVVVLDNGMYGTIRMHQEREYPG
HHHCCCCCEEEEEEECCCCEEEEHHHHHHHHHHHHEEEEEEECCCCEEEEEEECCCCCCC
RVSATELRNPDFAALARACGGHGERVERTEEFAPAFERGLASGLPAMIHCLVDPEALTPT
CCCHHHCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHHEEEECCHHCCCH
MTLAAIREKALAAQRG
HHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SGLPPRTGAQVLVDQLRAQGVERVTCVPGESYLAVLDALRDSGIDVLVCRQEGGAAIMA
CCCCCCCCHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHCCCEEEEEECCCCEEEEE
EAAGKLTGRPGICFVTRGPGATNASAGVHIARQDSTPMILFVGQIARRMRDREAFQEVDY
HHCCCCCCCCCEEEEECCCCCCCCCCCEEEEECCCCCEEEEHHHHHHHHHHHHHHHHCCH
RQMFGGVAKWAVEIDDAARIPEILARAFRVAMQGRPGPVVVALPEDMLDDVAAVPDAPRV
HHHHCCHHEEEEEECCHHHHHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHCCCCCCE
VPAAPAPSGDDVARLRAMLAEARRPVVLLGGSRWTPGAVAAMAAWAERHDLPVAVSFRRA
EECCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHCCCCEEEEEEHE
QLFPADHPNFAGELGIGPNPALLARIREADLLLMVGGRLSEMPAQSYGLLGIPDPGLPLV
EECCCCCCCCCCCCCCCCCHHHHHHHHCCCEEEEECCCHHHCCHHHCCEEECCCCCCCEE
HVHADAAELGRVYQPALAIEAAPETFCAVLPDFEATGTGRAAEAHAAYRAWSETPEPKPG
EEECCHHHHHHHHCCHHEEECCCHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCC
AFQYGAAITWLRERLPPDAVICNGAGNFATWVHRYYRFRAFGTQLAPTSGSMGYGLPAAV
CCHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHCCEECCCCCCCCCCCHHHH
MAKRSRPERIVVALAGDGDVMMTVQEFATAVQYGIAVVVVVLDNGMYGTIRMHQEREYPG
HHHCCCCCEEEEEEECCCCEEEEHHHHHHHHHHHHEEEEEEECCCCEEEEEEECCCCCCC
RVSATELRNPDFAALARACGGHGERVERTEEFAPAFERGLASGLPAMIHCLVDPEALTPT
CCCHHHCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHHEEEECCHHCCCH
MTLAAIREKALAAQRG
HHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3550695; 1379743; 9278503; 1995430; 6154938; 7015336; 3897211 [H]