Click here to switch to the map view.

The map label for this gene is nuoB [H]

Identifier: 170748477

GI number: 170748477

Start: 2189967

End: 2190554

Strand: Reverse

Name: nuoB [H]

Synonym: Mrad2831_2059

Alternate gene names: 170748477

Gene position: 2190554-2189967 (Counterclockwise)

Preceding gene: 170748478

Following gene: 170748476

Centisome position: 36.04

GC content: 68.37

Gene sequence:

>588_bases
ATGGCCCTGATCACCGACACCAACCGCGCCCCCGCGATCGCGCCCCAGCCGAAGGGGATCATCGATCCCAACACCGGCCG
CCCGATCGGCGCGGACGATCCGACCTTCCTGTCGATCAGCGACGAGCTGGCCGACCGGGGCTTCCTGCTCACCACCACGG
ACGAGCTGATCAACTGGGCCCGCACGGGCTCGCTGATGTGGATGACCTTCGGTCTCGCCTGCTGCGCGGTCGAGATGATG
CAGATGTCGATGCCGCGCTACGATTGCGAGCGATTCGGCTTCGCGCCCCGCGGCTCGCCCCGCCAGTCCGACGTGATGAT
CGTCGCCGGCACGCTGACCAACAAGATGGCCCCGGCGCTGCGCAAGGTCTACGACCAGATGCCGGAGCCGCGCTACGTCA
TCTCGATGGGCTCCTGCGCCAACGGCGGCGGCTACTATCACTACTCCTACTCGGTGGTGCGCGGCTGCGACCGGGTGGTG
CCGGTGGACATCTACGTGCCCGGCTGCCCGCCCACCGCCGAGGCGCTGCTCTACGGCGTGCTGCTGCTGCAGAAGAAGAT
CCGCCGGACCGGCACGATCGAGCGCTGA

Upstream 100 bases:

>100_bases
CGTGCTGACGGTCGGTTTCGTCTACGAGTGGCGCAAGGGCGCCCTCGAATGGGATTGACGTCCCGCCGCTCGCCGCCCGC
ACTGAATTCCTGAGTCGCCG

Downstream 100 bases:

>100_bases
GGAGCGGCCATGACGAACGGCATCTCGATCCTGCCCCACACCCAGCCGGTCTACGGCGACGCGGCCGTGGCTGCCATGGC
CGAGCGCGTCGTCGGCGCCC

Product: NADH dehydrogenase subunit B

Products: NA

Alternate protein names: NADH dehydrogenase I subunit B; NDH-1 subunit B [H]

Number of amino acids: Translated: 195; Mature: 194

Protein sequence:

>195_residues
MALITDTNRAPAIAPQPKGIIDPNTGRPIGADDPTFLSISDELADRGFLLTTTDELINWARTGSLMWMTFGLACCAVEMM
QMSMPRYDCERFGFAPRGSPRQSDVMIVAGTLTNKMAPALRKVYDQMPEPRYVISMGSCANGGGYYHYSYSVVRGCDRVV
PVDIYVPGCPPTAEALLYGVLLLQKKIRRTGTIER

Sequences:

>Translated_195_residues
MALITDTNRAPAIAPQPKGIIDPNTGRPIGADDPTFLSISDELADRGFLLTTTDELINWARTGSLMWMTFGLACCAVEMM
QMSMPRYDCERFGFAPRGSPRQSDVMIVAGTLTNKMAPALRKVYDQMPEPRYVISMGSCANGGGYYHYSYSVVRGCDRVV
PVDIYVPGCPPTAEALLYGVLLLQKKIRRTGTIER
>Mature_194_residues
ALITDTNRAPAIAPQPKGIIDPNTGRPIGADDPTFLSISDELADRGFLLTTTDELINWARTGSLMWMTFGLACCAVEMMQ
MSMPRYDCERFGFAPRGSPRQSDVMIVAGTLTNKMAPALRKVYDQMPEPRYVISMGSCANGGGYYHYSYSVVRGCDRVVP
VDIYVPGCPPTAEALLYGVLLLQKKIRRTGTIER

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG0377

COG function: function code C; NADH:ubiquinone oxidoreductase 20 kD subunit and related Fe-S oxidoreductases

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 20 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI187281616, Length=162, Percent_Identity=72.2222222222222, Blast_Score=250, Evalue=5e-67,
Organism=Escherichia coli, GI1788624, Length=148, Percent_Identity=46.6216216216216, Blast_Score=150, Evalue=4e-38,
Organism=Escherichia coli, GI1789074, Length=126, Percent_Identity=42.8571428571429, Blast_Score=105, Evalue=2e-24,
Organism=Escherichia coli, GI1788834, Length=123, Percent_Identity=40.650406504065, Blast_Score=100, Evalue=6e-23,
Organism=Caenorhabditis elegans, GI17509685, Length=173, Percent_Identity=64.1618497109827, Blast_Score=226, Evalue=5e-60,
Organism=Drosophila melanogaster, GI18859983, Length=145, Percent_Identity=75.8620689655172, Blast_Score=244, Evalue=2e-65,
Organism=Drosophila melanogaster, GI24642371, Length=145, Percent_Identity=75.8620689655172, Blast_Score=244, Evalue=2e-65,
Organism=Drosophila melanogaster, GI24651058, Length=141, Percent_Identity=76.5957446808511, Blast_Score=238, Evalue=2e-63,

Paralogues:

None

Copy number: 520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006137
- InterPro:   IPR006138
- InterPro:   IPR014406 [H]

Pfam domain/function: PF01058 Oxidored_q6 [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 21466; Mature: 21335

Theoretical pI: Translated: 7.74; Mature: 7.74

Prosite motif: PS01150 COMPLEX1_20K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
5.6 %Met     (Translated Protein)
8.7 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
5.2 %Met     (Mature Protein)
8.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALITDTNRAPAIAPQPKGIIDPNTGRPIGADDPTFLSISDELADRGFLLTTTDELINWA
CEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECHHHHCCCEEEEEHHHHHHHH
RTGSLMWMTFGLACCAVEMMQMSMPRYDCERFGFAPRGSPRQSDVMIVAGTLTNKMAPAL
HCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCCCCCCCCEEEEEECHHHHHHHHH
RKVYDQMPEPRYVISMGSCANGGGYYHYSYSVVRGCDRVVPVDIYVPGCPPTAEALLYGV
HHHHHHCCCCEEEEEECCCCCCCCEEEEEEHHHHCCCCEEEEEEEECCCCCHHHHHHHHH
LLLQKKIRRTGTIER
HHHHHHHHHCCCCCC
>Mature Secondary Structure 
ALITDTNRAPAIAPQPKGIIDPNTGRPIGADDPTFLSISDELADRGFLLTTTDELINWA
EEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECHHHHCCCEEEEEHHHHHHHH
RTGSLMWMTFGLACCAVEMMQMSMPRYDCERFGFAPRGSPRQSDVMIVAGTLTNKMAPAL
HCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCCCCCCCCEEEEEECHHHHHHHHH
RKVYDQMPEPRYVISMGSCANGGGYYHYSYSVVRGCDRVVPVDIYVPGCPPTAEALLYGV
HHHHHHCCCCEEEEEECCCCCCCCEEEEEEHHHHCCCCEEEEEEEECCCCCHHHHHHHHH
LLLQKKIRRTGTIER
HHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA