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The map label for this gene is yusP [H]

Identifier: 170748427

GI number: 170748427

Start: 2133888

End: 2135426

Strand: Reverse

Name: yusP [H]

Synonym: Mrad2831_2009

Alternate gene names: 170748427

Gene position: 2135426-2133888 (Counterclockwise)

Preceding gene: 170748429

Following gene: 170748426

Centisome position: 35.13

GC content: 72.25

Gene sequence:

>1539_bases
GTGGCCAGTCCCGTCGCCGTCACCGAGGTCCAGCCGCAAACCCGGGCGCGGCCGGCCGGCCCGCAGGCCGCGCTCAGCCC
CGGCGAGATCCGCGCCATCGTCTACGGGCTGATGACCGCGATGCTGCTCGCGGCCCTCGACCAGACCATCGTGGCGACCG
CCATGCCGACCATCGGCCTCGACCTCGGCGACGCCGCGAACCTGCCCTGGATCGTCACCGCCTACCTGCTGGCCTCCACG
GCGGTGACCCCGCTCTACGGCAAGCTCAGCGACATCCACGGCCGGCGGATCATGCTGCTGATCGCCATCGCGACCTTCGT
GGTCGGCTCGCTCGCCTGCGCGCTCGCGCCCACGATGGTGGCGCTGGCGCTCGCCCGGGGCCTCCAGGGCGTCGGCGGCG
GCGGGCTGATCGCCCTGGCGCAGACCATCCTGGCCGACATCATGTCGCCCAAGGAGCGGGCGCGCTACCAGGTGGTGATC
GCGGGCGTGTTCGTCACGGCCTCGGTGGCGGGGCCGCTGCTCGGCGGCTTCTTCGCCCAGCACCTGCACTGGTCGCTGAT
CTTCTGGATCAACCTGCCGATCGGCTGCCTCGCCTTCGCGCTCACCAACGCCAACCTGAAGCGCCTGCCGCGCCACGAGC
GCCGCCACCGGCTGGACTATCCCGGCGCCGCCCTGATGGTGGCGGGCTCCGTCACCCTCCTGCTGGCCCTGAGCTGGGGC
GGGGTGCGCTATCCCTGGGACTCGGCGCCGGTCCTCGCCCTCCTCGCCGGGGCGGCCGTGCTGGGGGGCGGCTTCGCGGC
GCGGCTCGCCACGGCGCCGGAGCCGCTGATCCCCACGGAGGTGCTGAGGGACCGGGTGGTCTACAGCGCGACGCTGGCGG
CCTGCTTCGCCATGGGCACCTTCATCGGGCTCACCATCTACGTGCCGATCTTCCTCGAAGGGGTGATCGGGCTCTCGGCG
AGCGAGTCCGGCGTGGCCCTGGTGCCGCTGATGATCGGCACCGTCACGGGCGCGACCCTGTCGGGCCGGTCGATGCTGCA
TTTCCGGCACTACAAGCGGGTACCACTGGCGATGATGTGCGTCAGTCTCGCCTGCTGCGCCACCATCGCCTGGCAGGGCC
GGGCGCTGCCCTTCTGGCTCATGGAGGTGCTGTTCGCGCTGCTCTCCATGGGAATCGGGACGATCCTCCCGCTCTCGACC
ATCTCGATCCAGAACGCCGTGGAGACGCACCAGCTCGGCATCGCCACCGCCGCGATGAACTTCTTCCGCTCCCTCGGCGG
GGCGCTGATCGTGGCGGCCTTCGGGACGATCGTGCTCGGCGGCGCCGCGGGCGGGGCAGGGGGAGGGGCGCACGACGTGG
AGAGCCTGATCCGCGGCGCGGACCCGGCCCAGCTCGCCCTGACTTTCCGCTACGTCTTCCTGGCCGCCTGCCTCGGCCTG
CTCGGCGCCTTCACGTTCCTGGCGCTGATGGAGGAGCGGCCCCTGCGCGAGCGCACGTCGCCGAAGATGGCCGCCGAGAC
GCCGGAGGCCTCCACCTGA

Upstream 100 bases:

>100_bases
CCGCGACCGCGGTCCGTCCGCACACCTCCTCATGCCGCCCCAGGCCCCGTCCGCCCCGGACGGTCGGCCGGCACGGTCCG
GATCAGAGGCTCTCAGACAC

Downstream 100 bases:

>100_bases
CGGCCCCCGGGGTGGACTTCTGCCGATCCCGCGACGCCGGACCGGGCCGGGATCCCGTCACCGCGCACGCGGGCGGGGCC
GAGACGGGAGACCGCTCCGG

Product: major facilitator transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 512; Mature: 511

Protein sequence:

>512_residues
MASPVAVTEVQPQTRARPAGPQAALSPGEIRAIVYGLMTAMLLAALDQTIVATAMPTIGLDLGDAANLPWIVTAYLLAST
AVTPLYGKLSDIHGRRIMLLIAIATFVVGSLACALAPTMVALALARGLQGVGGGGLIALAQTILADIMSPKERARYQVVI
AGVFVTASVAGPLLGGFFAQHLHWSLIFWINLPIGCLAFALTNANLKRLPRHERRHRLDYPGAALMVAGSVTLLLALSWG
GVRYPWDSAPVLALLAGAAVLGGGFAARLATAPEPLIPTEVLRDRVVYSATLAACFAMGTFIGLTIYVPIFLEGVIGLSA
SESGVALVPLMIGTVTGATLSGRSMLHFRHYKRVPLAMMCVSLACCATIAWQGRALPFWLMEVLFALLSMGIGTILPLST
ISIQNAVETHQLGIATAAMNFFRSLGGALIVAAFGTIVLGGAAGGAGGGAHDVESLIRGADPAQLALTFRYVFLAACLGL
LGAFTFLALMEERPLRERTSPKMAAETPEAST

Sequences:

>Translated_512_residues
MASPVAVTEVQPQTRARPAGPQAALSPGEIRAIVYGLMTAMLLAALDQTIVATAMPTIGLDLGDAANLPWIVTAYLLAST
AVTPLYGKLSDIHGRRIMLLIAIATFVVGSLACALAPTMVALALARGLQGVGGGGLIALAQTILADIMSPKERARYQVVI
AGVFVTASVAGPLLGGFFAQHLHWSLIFWINLPIGCLAFALTNANLKRLPRHERRHRLDYPGAALMVAGSVTLLLALSWG
GVRYPWDSAPVLALLAGAAVLGGGFAARLATAPEPLIPTEVLRDRVVYSATLAACFAMGTFIGLTIYVPIFLEGVIGLSA
SESGVALVPLMIGTVTGATLSGRSMLHFRHYKRVPLAMMCVSLACCATIAWQGRALPFWLMEVLFALLSMGIGTILPLST
ISIQNAVETHQLGIATAAMNFFRSLGGALIVAAFGTIVLGGAAGGAGGGAHDVESLIRGADPAQLALTFRYVFLAACLGL
LGAFTFLALMEERPLRERTSPKMAAETPEAST
>Mature_511_residues
ASPVAVTEVQPQTRARPAGPQAALSPGEIRAIVYGLMTAMLLAALDQTIVATAMPTIGLDLGDAANLPWIVTAYLLASTA
VTPLYGKLSDIHGRRIMLLIAIATFVVGSLACALAPTMVALALARGLQGVGGGGLIALAQTILADIMSPKERARYQVVIA
GVFVTASVAGPLLGGFFAQHLHWSLIFWINLPIGCLAFALTNANLKRLPRHERRHRLDYPGAALMVAGSVTLLLALSWGG
VRYPWDSAPVLALLAGAAVLGGGFAARLATAPEPLIPTEVLRDRVVYSATLAACFAMGTFIGLTIYVPIFLEGVIGLSAS
ESGVALVPLMIGTVTGATLSGRSMLHFRHYKRVPLAMMCVSLACCATIAWQGRALPFWLMEVLFALLSMGIGTILPLSTI
SIQNAVETHQLGIATAAMNFFRSLGGALIVAAFGTIVLGGAAGGAGGGAHDVESLIRGADPAQLALTFRYVFLAACLGLL
GAFTFLALMEERPLRERTSPKMAAETPEAST

Specific function: Translocase That Confers Resistance To Substances Of High Hydrophobicity. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=417, Percent_Identity=26.6187050359712, Blast_Score=117, Evalue=2e-27,
Organism=Escherichia coli, GI1788710, Length=424, Percent_Identity=25.2358490566038, Blast_Score=108, Evalue=9e-25,
Organism=Escherichia coli, GI87081983, Length=319, Percent_Identity=28.8401253918495, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1788392, Length=367, Percent_Identity=28.3378746594005, Blast_Score=89, Evalue=7e-19,
Organism=Escherichia coli, GI1790195, Length=377, Percent_Identity=24.4031830238727, Blast_Score=83, Evalue=4e-17,
Organism=Escherichia coli, GI1790146, Length=147, Percent_Identity=29.9319727891156, Blast_Score=63, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6323735, Length=443, Percent_Identity=27.5395033860045, Blast_Score=129, Evalue=2e-30,
Organism=Saccharomyces cerevisiae, GI6325455, Length=246, Percent_Identity=31.3008130081301, Blast_Score=122, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6322958, Length=351, Percent_Identity=29.0598290598291, Blast_Score=120, Evalue=4e-28,
Organism=Saccharomyces cerevisiae, GI6321663, Length=351, Percent_Identity=26.7806267806268, Blast_Score=108, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6319770, Length=375, Percent_Identity=26.6666666666667, Blast_Score=100, Evalue=6e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR005829
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 53375; Mature: 53244

Theoretical pI: Translated: 8.84; Mature: 8.84

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASPVAVTEVQPQTRARPAGPQAALSPGEIRAIVYGLMTAMLLAALDQTIVATAMPTIGL
CCCCCEEEECCCHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
DLGDAANLPWIVTAYLLASTAVTPLYGKLSDIHGRRIMLLIAIATFVVGSLACALAPTMV
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
ALALARGLQGVGGGGLIALAQTILADIMSPKERARYQVVIAGVFVTASVAGPLLGGFFAQ
HHHHHHCCCCCCCCHHHHHHHHHHHHHHCHHHHCCEEEEEEHHHHHHHHHHHHHHHHHHH
HLHWSLIFWINLPIGCLAFALTNANLKRLPRHERRHRLDYPGAALMVAGSVTLLLALSWG
HHHHHHHHEECCCHHHHHHHHHCCCHHHCCCHHHHCCCCCCCHHHHHHHHHHHHHEECCC
GVRYPWDSAPVLALLAGAAVLGGGFAARLATAPEPLIPTEVLRDRVVYSATLAACFAMGT
CEECCCCCCHHHHHHHHHHHHCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
FIGLTIYVPIFLEGVIGLSASESGVALVPLMIGTVTGATLSGRSMLHFRHYKRVPLAMMC
HHHHHHHHHHHHHHHHCCCCCCCCCEEHHHHHHHHCCCCCCCCHHHHHHHHCCCHHHHHH
VSLACCATIAWQGRALPFWLMEVLFALLSMGIGTILPLSTISIQNAVETHQLGIATAAMN
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FFRSLGGALIVAAFGTIVLGGAAGGAGGGAHDVESLIRGADPAQLALTFRYVFLAACLGL
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
LGAFTFLALMEERPLRERTSPKMAAETPEAST
HHHHHHHHHHHCCCCHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
ASPVAVTEVQPQTRARPAGPQAALSPGEIRAIVYGLMTAMLLAALDQTIVATAMPTIGL
CCCCEEEECCCHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
DLGDAANLPWIVTAYLLASTAVTPLYGKLSDIHGRRIMLLIAIATFVVGSLACALAPTMV
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
ALALARGLQGVGGGGLIALAQTILADIMSPKERARYQVVIAGVFVTASVAGPLLGGFFAQ
HHHHHHCCCCCCCCHHHHHHHHHHHHHHCHHHHCCEEEEEEHHHHHHHHHHHHHHHHHHH
HLHWSLIFWINLPIGCLAFALTNANLKRLPRHERRHRLDYPGAALMVAGSVTLLLALSWG
HHHHHHHHEECCCHHHHHHHHHCCCHHHCCCHHHHCCCCCCCHHHHHHHHHHHHHEECCC
GVRYPWDSAPVLALLAGAAVLGGGFAARLATAPEPLIPTEVLRDRVVYSATLAACFAMGT
CEECCCCCCHHHHHHHHHHHHCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
FIGLTIYVPIFLEGVIGLSASESGVALVPLMIGTVTGATLSGRSMLHFRHYKRVPLAMMC
HHHHHHHHHHHHHHHHCCCCCCCCCEEHHHHHHHHCCCCCCCCHHHHHHHHCCCHHHHHH
VSLACCATIAWQGRALPFWLMEVLFALLSMGIGTILPLSTISIQNAVETHQLGIATAAMN
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FFRSLGGALIVAAFGTIVLGGAAGGAGGGAHDVESLIRGADPAQLALTFRYVFLAACLGL
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
LGAFTFLALMEERPLRERTSPKMAAETPEAST
HHHHHHHHHHHCCCCHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]