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The map label for this gene is ksgA

Identifier: 170746986

GI number: 170746986

Start: 592327

End: 593205

Strand: Reverse

Name: ksgA

Synonym: Mrad2831_0552

Alternate gene names: 170746986

Gene position: 593205-592327 (Counterclockwise)

Preceding gene: 170746987

Following gene: 170746982

Centisome position: 9.76

GC content: 75.77

Gene sequence:

>879_bases
ATGAGCGCCGGGGACGGGCTTCCGCCGCTGCGCGAGGTGGTCGCCCGCCACGGCCTGGAGCCGAAGAAGGCGCTCGGCCA
GAACTTCCTCTACGACCTCAACCTGACCGGGCGGATCGCCCGGGCCGCCGGTCCCCTCGCGGGCGTGACGGTGGTGGAGG
TCGGTCCGGGACCCGGCGGCCTCACGCGCGCGCTCCTCGCCGAGGGGGCCGCCCGCGTGGTGGCGATCGAGCGCGACCCG
CGGGCGCTCCCGGCGCTCGCCGAGATCGCCGCCCACTATCCGGGACGCCTCGAGGTGGTCGACGCCGACGCCCTCGCCTT
CGATCCGCGGCCGCTGGTGGGCGACGCCCCGGCGCGGATCGTCGCCAATCTGCCCTACAATGTCGGCACGGCCCTGCTCA
CCGGCTGGCTCGACGGGGAGGCTTGGCCGCCCTGGTGGGATCAGGCCGTGCTGATGTTCCAGCGGGAGGTGGCCGAGCGG
ATCGTCGCCGGCCCGGAGGAGCGGGCCGATTACGGCCGGCTCGGCGTCCTGTGCGGCTGGCGCACGGAGGCCGAGATCCT
GTTCGACGTCAGCCCCTCGGCTTTCGTGCCGCCGCCGAAGGTGACGTCGAGCGTGGTTCGGCTGGTGCCCCGCGCCCAGC
CCCTGCCGTGCCGCGCCGGCGCGCTCGAGGCCGTCACCCGCGCGGCCTTCGGCCAGCGCCGGAAGATGCTGCGCCAGAGC
CTCAAGGCCTTGACCCCCGCGGCCGGCGACCTGCTGGCCGCCGCCGGCCTGTCCGAGACCGCGCGGGCCGAGGAGATCCC
GGTGGCGGGCTTCGTCGATCTCGCCAATCGGTGGGACGCGCACAGAAAGGCCGGCGCCCCGGTGGGGACGCCGGCCTGA

Upstream 100 bases:

>100_bases
GGCGGGGCCGAGATCATCCCGTTCAGCGTCTATGCCGGCCGGCCGCGCCCTGCGGGGGCCCGCCATTTCGATCCCGAGGA
CGAGCTGTGAGGGACCCAGC

Downstream 100 bases:

>100_bases
ATGAGCATCGTGACTCCGGCCCGAAGGCCGGAGCGCGATCGGATCACCGGGTCTGCTGGATCGCCGAGAGCACCCAGCCG
CGGCCGGGCTGGCGCAGGAA

Product: dimethyladenosine transferase

Products: NA

Alternate protein names: 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase; 16S rRNA dimethyladenosine transferase; 16S rRNA dimethylase; S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase

Number of amino acids: Translated: 292; Mature: 291

Protein sequence:

>292_residues
MSAGDGLPPLREVVARHGLEPKKALGQNFLYDLNLTGRIARAAGPLAGVTVVEVGPGPGGLTRALLAEGAARVVAIERDP
RALPALAEIAAHYPGRLEVVDADALAFDPRPLVGDAPARIVANLPYNVGTALLTGWLDGEAWPPWWDQAVLMFQREVAER
IVAGPEERADYGRLGVLCGWRTEAEILFDVSPSAFVPPPKVTSSVVRLVPRAQPLPCRAGALEAVTRAAFGQRRKMLRQS
LKALTPAAGDLLAAAGLSETARAEEIPVAGFVDLANRWDAHRKAGAPVGTPA

Sequences:

>Translated_292_residues
MSAGDGLPPLREVVARHGLEPKKALGQNFLYDLNLTGRIARAAGPLAGVTVVEVGPGPGGLTRALLAEGAARVVAIERDP
RALPALAEIAAHYPGRLEVVDADALAFDPRPLVGDAPARIVANLPYNVGTALLTGWLDGEAWPPWWDQAVLMFQREVAER
IVAGPEERADYGRLGVLCGWRTEAEILFDVSPSAFVPPPKVTSSVVRLVPRAQPLPCRAGALEAVTRAAFGQRRKMLRQS
LKALTPAAGDLLAAAGLSETARAEEIPVAGFVDLANRWDAHRKAGAPVGTPA
>Mature_291_residues
SAGDGLPPLREVVARHGLEPKKALGQNFLYDLNLTGRIARAAGPLAGVTVVEVGPGPGGLTRALLAEGAARVVAIERDPR
ALPALAEIAAHYPGRLEVVDADALAFDPRPLVGDAPARIVANLPYNVGTALLTGWLDGEAWPPWWDQAVLMFQREVAERI
VAGPEERADYGRLGVLCGWRTEAEILFDVSPSAFVPPPKVTSSVVRLVPRAQPLPCRAGALEAVTRAAFGQRRKMLRQSL
KALTPAAGDLLAAAGLSETARAEEIPVAGFVDLANRWDAHRKAGAPVGTPA

Specific function: Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits

COG id: COG0030

COG function: function code J; Dimethyladenosine transferase (rRNA methylation)

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily

Homologues:

Organism=Homo sapiens, GI156415992, Length=301, Percent_Identity=35.8803986710963, Blast_Score=162, Evalue=3e-40,
Organism=Homo sapiens, GI7657198, Length=228, Percent_Identity=34.2105263157895, Blast_Score=103, Evalue=1e-22,
Organism=Escherichia coli, GI1786236, Length=259, Percent_Identity=37.0656370656371, Blast_Score=141, Evalue=4e-35,
Organism=Caenorhabditis elegans, GI25141369, Length=309, Percent_Identity=31.7152103559871, Blast_Score=147, Evalue=5e-36,
Organism=Caenorhabditis elegans, GI25146882, Length=214, Percent_Identity=35.0467289719626, Blast_Score=103, Evalue=9e-23,
Organism=Saccharomyces cerevisiae, GI6324989, Length=231, Percent_Identity=30.3030303030303, Blast_Score=71, Evalue=2e-13,
Organism=Drosophila melanogaster, GI21357273, Length=306, Percent_Identity=35.2941176470588, Blast_Score=144, Evalue=1e-34,
Organism=Drosophila melanogaster, GI21358017, Length=261, Percent_Identity=33.7164750957854, Blast_Score=103, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMA_METRJ (B1LVB8)

Other databases:

- EMBL:   CP001001
- RefSeq:   YP_001753246.1
- ProteinModelPortal:   B1LVB8
- SMR:   B1LVB8
- GeneID:   6136565
- GenomeReviews:   CP001001_GR
- KEGG:   mrd:Mrad2831_0552
- HOGENOM:   HBG319664
- OMA:   GVLCGWR
- ProtClustDB:   PRK00274
- GO:   GO:0005737
- HAMAP:   MF_00607
- InterPro:   IPR023165
- InterPro:   IPR020596
- InterPro:   IPR001737
- InterPro:   IPR020598
- InterPro:   IPR011530
- Gene3D:   G3DSA:1.10.8.100
- PANTHER:   PTHR11727
- SMART:   SM00650
- TIGRFAMs:   TIGR00755

Pfam domain/function: PF00398 RrnaAD

EC number: =2.1.1.182

Molecular weight: Translated: 30817; Mature: 30686

Theoretical pI: Translated: 7.52; Mature: 7.52

Prosite motif: PS01131 RRNA_A_DIMETH

Important sites: BINDING 28-28 BINDING 30-30 BINDING 55-55 BINDING 77-77 BINDING 103-103 BINDING 123-123

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAGDGLPPLREVVARHGLEPKKALGQNFLYDLNLTGRIARAAGPLAGVTVVEVGPGPGG
CCCCCCCCHHHHHHHHCCCCHHHHHCCCCEEECCCCCHHHHHCCCCCCEEEEEECCCCCH
LTRALLAEGAARVVAIERDPRALPALAEIAAHYPGRLEVVDADALAFDPRPLVGDAPARI
HHHHHHHCCCEEEEEECCCCCHHHHHHHHHHCCCCEEEEEECCCEEECCCCCCCCCCHHE
VANLPYNVGTALLTGWLDGEAWPPWWDQAVLMFQREVAERIVAGPEERADYGRLGVLCGW
EECCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHCCCCCEEEEECC
RTEAEILFDVSPSAFVPPPKVTSSVVRLVPRAQPLPCRAGALEAVTRAAFGQRRKMLRQS
CCCCEEEEECCCCCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
LKALTPAAGDLLAAAGLSETARAEEIPVAGFVDLANRWDAHRKAGAPVGTPA
HHHHCCCHHHHHHHCCCCHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
SAGDGLPPLREVVARHGLEPKKALGQNFLYDLNLTGRIARAAGPLAGVTVVEVGPGPGG
CCCCCCCHHHHHHHHCCCCHHHHHCCCCEEECCCCCHHHHHCCCCCCEEEEEECCCCCH
LTRALLAEGAARVVAIERDPRALPALAEIAAHYPGRLEVVDADALAFDPRPLVGDAPARI
HHHHHHHCCCEEEEEECCCCCHHHHHHHHHHCCCCEEEEEECCCEEECCCCCCCCCCHHE
VANLPYNVGTALLTGWLDGEAWPPWWDQAVLMFQREVAERIVAGPEERADYGRLGVLCGW
EECCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHCCCCCEEEEECC
RTEAEILFDVSPSAFVPPPKVTSSVVRLVPRAQPLPCRAGALEAVTRAAFGQRRKMLRQS
CCCCEEEEECCCCCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
LKALTPAAGDLLAAAGLSETARAEEIPVAGFVDLANRWDAHRKAGAPVGTPA
HHHHCCCHHHHHHHCCCCHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA