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The map label for this gene is ttgG [H]

Identifier: 170746734

GI number: 170746734

Start: 309907

End: 311067

Strand: Reverse

Name: ttgG [H]

Synonym: Mrad2831_0285

Alternate gene names: 170746734

Gene position: 311067-309907 (Counterclockwise)

Preceding gene: 170746736

Following gene: 170746733

Centisome position: 5.12

GC content: 74.16

Gene sequence:

>1161_bases
ATGTCGATCTCGACCGCGCGCGGCTTCCGGCGCCCGACCCTCGTCGCGCTCGTGGTGAGCGCGTCGCTGAGCGGCTGCAA
GCCCGCCGCCACCGACACGGCCGCGGTCGAGACGCCACCGCCGGACGTGTCGGTCGCGACCGTGACGCCCGCGACCGTGC
CGCGCCTCTTCGACCTGCCGGGGCGCCTCGCCTCGACGCGGGTGGCGGAGGTCCGGGCCCAGGTTTCGGGCATCATCCGG
GAGCGCGCGTTCGAGGAAGGCAGCCTCGTCGAGAAGGACGACGTCCTCTTCCGCATCGACCCGGCCGTCTACAAGGCCGA
GGTCGACGCGAAGGCCGCGTCCCTCGCCCGAGCCGAGGCGTCGCGGGTGCAGGCGCTGAAGCAGTCGGAGCGGACCGAGA
CGCTGCTGGAGCGGCAGGCGGCCAGCACCGCCCAGCACGACGTCGCCGTCGCGACCCTGCGACAGGCCGAGGCCGACGTG
GCGAGTGCCAGGGCGCAGCTTGACCGGGCCCGGATCGACCTGGACTTCGCCACCGTTCGAGCACCCATCGGCGGGCGGAT
CGGGCGGGCGCTCGTGACGGAGGGCGCGCTCGTCGGCCAGGGCGAGGCGACGAAGCTCGCGGTCATCCAGCAGGTCGACC
CGATCTTCGCCGACTTCAACGCCTCCGCGTCCGATTACGGGTTGCTGGCGGACCGCGGTGGGGCCGGAGGCGCCGAGGTC
CGGCTGGTCCACGCGGACGGCACCCTGCACGCCGATCCCGGCAAGCTCCTGTTCTCGGACGTGAGCGTCGATCCGGGCAC
CGGTCAGGTCAGCCTGCGCGCCGCCTTCGCAAACCCGCGTCTCTCCCTGCTGCCCGGCGCCTATGTTCGGATCCGCGTCG
CGCGCGGGGTGCAGCCGGGCGCCGTCATCGTGCCCGCGCAGGCGATCCAGCGCTCCAGCGCCGGCGAGGCCCAGGCCTTC
GTGGTTGGCGCCGACCGGTTGCTGGCCCTGCGGCCCCTGCGCACCGGCGCGATGACCGAGGTCGGCTGGATCGTCGAGGA
CGGGCTGAAGCCCGGCGAGACCGTCGTCGTCGAGGGCTTCCAGAAGATCCATCCCGGCCTGGCGGTCAATCCGGTGCCGT
GGGCGGGCGGCCGCACGGCCACCGCCGCCCCGCAGCCCTGA

Upstream 100 bases:

>100_bases
GGAGCGGCGCGACACCCTCGTGGCGCCGACGCGGGCCGGATTTCAGGGGCCGGCGGACCGGTGTGCCGGATCCCGCTCAG
TGGAAGGAGCATTTTGGTCC

Downstream 100 bases:

>100_bases
CTCCGTCACGCAGGCTCGACCCGCCATGACCCGCTTCTTCATCGAGCGCCCGATCTTCGCCTGGGCGATCTCCATCTTCA
TCGTGCTGGTCGGCGCGATC

Product: RND family efflux transporter MFP subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 386; Mature: 385

Protein sequence:

>386_residues
MSISTARGFRRPTLVALVVSASLSGCKPAATDTAAVETPPPDVSVATVTPATVPRLFDLPGRLASTRVAEVRAQVSGIIR
ERAFEEGSLVEKDDVLFRIDPAVYKAEVDAKAASLARAEASRVQALKQSERTETLLERQAASTAQHDVAVATLRQAEADV
ASARAQLDRARIDLDFATVRAPIGGRIGRALVTEGALVGQGEATKLAVIQQVDPIFADFNASASDYGLLADRGGAGGAEV
RLVHADGTLHADPGKLLFSDVSVDPGTGQVSLRAAFANPRLSLLPGAYVRIRVARGVQPGAVIVPAQAIQRSSAGEAQAF
VVGADRLLALRPLRTGAMTEVGWIVEDGLKPGETVVVEGFQKIHPGLAVNPVPWAGGRTATAAPQP

Sequences:

>Translated_386_residues
MSISTARGFRRPTLVALVVSASLSGCKPAATDTAAVETPPPDVSVATVTPATVPRLFDLPGRLASTRVAEVRAQVSGIIR
ERAFEEGSLVEKDDVLFRIDPAVYKAEVDAKAASLARAEASRVQALKQSERTETLLERQAASTAQHDVAVATLRQAEADV
ASARAQLDRARIDLDFATVRAPIGGRIGRALVTEGALVGQGEATKLAVIQQVDPIFADFNASASDYGLLADRGGAGGAEV
RLVHADGTLHADPGKLLFSDVSVDPGTGQVSLRAAFANPRLSLLPGAYVRIRVARGVQPGAVIVPAQAIQRSSAGEAQAF
VVGADRLLALRPLRTGAMTEVGWIVEDGLKPGETVVVEGFQKIHPGLAVNPVPWAGGRTATAAPQP
>Mature_385_residues
SISTARGFRRPTLVALVVSASLSGCKPAATDTAAVETPPPDVSVATVTPATVPRLFDLPGRLASTRVAEVRAQVSGIIRE
RAFEEGSLVEKDDVLFRIDPAVYKAEVDAKAASLARAEASRVQALKQSERTETLLERQAASTAQHDVAVATLRQAEADVA
SARAQLDRARIDLDFATVRAPIGGRIGRALVTEGALVGQGEATKLAVIQQVDPIFADFNASASDYGLLADRGGAGGAEVR
LVHADGTLHADPGKLLFSDVSVDPGTGQVSLRAAFANPRLSLLPGAYVRIRVARGVQPGAVIVPAQAIQRSSAGEAQAFV
VGADRLLALRPLRTGAMTEVGWIVEDGLKPGETVVVEGFQKIHPGLAVNPVPWAGGRTATAAPQP

Specific function: The periplasmic linker component of an organic solvent efflux pump. Involved in export of a number of organic solvents, including toluene and styrene. This is the most important solvent efflux pump in this strain, although it can export AMP and some antib

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Lipid-anchor (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1786668, Length=378, Percent_Identity=41.2698412698413, Blast_Score=250, Evalue=1e-67,
Organism=Escherichia coli, GI1789665, Length=371, Percent_Identity=39.3530997304582, Blast_Score=247, Evalue=9e-67,
Organism=Escherichia coli, GI1789929, Length=384, Percent_Identity=35.15625, Blast_Score=226, Evalue=3e-60,
Organism=Escherichia coli, GI87082036, Length=335, Percent_Identity=25.6716417910448, Blast_Score=111, Evalue=1e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 40196; Mature: 40065

Theoretical pI: Translated: 7.68; Mature: 7.68

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
0.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
0.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSISTARGFRRPTLVALVVSASLSGCKPAATDTAAVETPPPDVSVATVTPATVPRLFDLP
CCCCCCCCCCCCHHEEEEEHHCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCHHHHCCC
GRLASTRVAEVRAQVSGIIRERAFEEGSLVEKDDVLFRIDPAVYKAEVDAKAASLARAEA
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCHHEEECCCHHHHHHHHHHH
SRVQALKQSERTETLLERQAASTAQHDVAVATLRQAEADVASARAQLDRARIDLDFATVR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEHHEEE
APIGGRIGRALVTEGALVGQGEATKLAVIQQVDPIFADFNASASDYGLLADRGGAGGAEV
CCCCCHHHHHHHHCCCEECCCCCCEEEEHHHCCCHHCCCCCCCCCCCEEECCCCCCCCEE
RLVHADGTLHADPGKLLFSDVSVDPGTGQVSLRAAFANPRLSLLPGAYVRIRVARGVQPG
EEEEECCEEECCCCCEEEEECCCCCCCCEEEEEEEECCCCEEECCCCEEEEEEECCCCCC
AVIVPAQAIQRSSAGEAQAFVVGADRLLALRPLRTGAMTEVGWIVEDGLKPGETVVVEGF
EEEECHHHHHCCCCCCCEEEEEECCCEEEEECCCCCCCHHHCCCEECCCCCCCEEEEECH
QKIHPGLAVNPVPWAGGRTATAAPQP
HHHCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SISTARGFRRPTLVALVVSASLSGCKPAATDTAAVETPPPDVSVATVTPATVPRLFDLP
CCCCCCCCCCCHHEEEEEHHCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCHHHHCCC
GRLASTRVAEVRAQVSGIIRERAFEEGSLVEKDDVLFRIDPAVYKAEVDAKAASLARAEA
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCHHEEECCCHHHHHHHHHHH
SRVQALKQSERTETLLERQAASTAQHDVAVATLRQAEADVASARAQLDRARIDLDFATVR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEHHEEE
APIGGRIGRALVTEGALVGQGEATKLAVIQQVDPIFADFNASASDYGLLADRGGAGGAEV
CCCCCHHHHHHHHCCCEECCCCCCEEEEHHHCCCHHCCCCCCCCCCCEEECCCCCCCCEE
RLVHADGTLHADPGKLLFSDVSVDPGTGQVSLRAAFANPRLSLLPGAYVRIRVARGVQPG
EEEEECCEEECCCCCEEEEECCCCCCCCEEEEEEEECCCCEEECCCCEEEEEEECCCCCC
AVIVPAQAIQRSSAGEAQAFVVGADRLLALRPLRTGAMTEVGWIVEDGLKPGETVVVEGF
EEEECHHHHHCCCCCCCEEEEEECCCEEEEECCCCCCCHHHCCCEECCCCCCCEEEEECH
QKIHPGLAVNPVPWAGGRTATAAPQP
HHHCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11395460 [H]