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The map label for this gene is yafC [H]

Identifier: 170746683

GI number: 170746683

Start: 250622

End: 251512

Strand: Reverse

Name: yafC [H]

Synonym: Mrad2831_0234

Alternate gene names: 170746683

Gene position: 251512-250622 (Counterclockwise)

Preceding gene: 170746685

Following gene: 170746681

Centisome position: 4.14

GC content: 75.08

Gene sequence:

>891_bases
ATGGACCTCTCGGCGCTCGCCGACTTCAACCTCGTCGCCACTCACGGCGGCTTCGGCCGCGCCGCCCGGGCGAGCGGGCG
GGCGAAGGCGACGCTGTCGCGCCGCGTGTCCGAGCTCGAGGCGAGCCTCGGGGCGCGGTTGATCGAGCGGGGCGAGCGCA
GCCTGCGCCTGACCGAGGCGGGCGCGCTCCTCCATGCCCGGACGGGGCCGCTGCTCAGCGAGATCGCGGAGGCCGGCGCG
GTGGTGGGTGGCGGCCTGGACCGGCCCCGTGGGCGCCTCCGGGTGAGTGCTCCGCTCCTCCTGTCCGACACGCAGCTCGG
CCGCGTCGCCGCGGACTTCGCCCGCGCCTATCCCGAAGTCGAGCTGGAGATCTGCGCCGAGGACCGGTTCGTCGATCCGA
TCGAGGAGGGTTTCGACGTGATCATCCGGGTCAATCCGAAGCCGGACGAGCGGCTCGTCGGGCGCTGCGTCCTGCGGGAC
GAGCTCTGGCTGGTGGCGCCGCCGGATCTCCCGCGCCCCGATACGTCGGCCGCGAGCGACGTGACGCCTGTGCCGGCGGC
GGTCCGCTGGACGCCGCGTCCGGAGGAGGCGTGGCAGGTCCATGATGGCCGGACACGCCGCGCCTTCGCGCCCGTGCCGG
TCCTGCGGCTGTCCTCGCTGCCGACGCTGCGCGACGCGGTGGTGGCGGGCGCCGGCGCCGCCCTGCTGCCGCGCTCGCTG
GTCGGGGGTGACGTGGCGGCCGGACGGCTGGCCTGTTGGGGCTGGCTCGAGGGGCCGCCGACGGAGCTGTGGGCGCTCCA
CACGTCCCGACGTCTGGTCAGCCCCAAGGTGAACGCCTTCGTGGCCCACCTGGCGACCGCGCTGTCGACGGATCTGTCCG
CCAACCGATAG

Upstream 100 bases:

>100_bases
AGGCCCGGGGCGGGCCGCCGATGTCCCGAAGATGCGCCGTTCCTATTCTGAGCCATAGATGGCAGGATCGGGACTGGGTG
TCCCGCATATGGAACAGCTT

Downstream 100 bases:

>100_bases
ACCTTCCGTCCGGGACGAGGGGGCGCCGGGCAAGGCCGTCGCGCGCGTGCCGGACGCGTCCCTATTCGCGGTTCAGCGCG
CCGTCCGGGCGGAACGGTTC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 296; Mature: 296

Protein sequence:

>296_residues
MDLSALADFNLVATHGGFGRAARASGRAKATLSRRVSELEASLGARLIERGERSLRLTEAGALLHARTGPLLSEIAEAGA
VVGGGLDRPRGRLRVSAPLLLSDTQLGRVAADFARAYPEVELEICAEDRFVDPIEEGFDVIIRVNPKPDERLVGRCVLRD
ELWLVAPPDLPRPDTSAASDVTPVPAAVRWTPRPEEAWQVHDGRTRRAFAPVPVLRLSSLPTLRDAVVAGAGAALLPRSL
VGGDVAAGRLACWGWLEGPPTELWALHTSRRLVSPKVNAFVAHLATALSTDLSANR

Sequences:

>Translated_296_residues
MDLSALADFNLVATHGGFGRAARASGRAKATLSRRVSELEASLGARLIERGERSLRLTEAGALLHARTGPLLSEIAEAGA
VVGGGLDRPRGRLRVSAPLLLSDTQLGRVAADFARAYPEVELEICAEDRFVDPIEEGFDVIIRVNPKPDERLVGRCVLRD
ELWLVAPPDLPRPDTSAASDVTPVPAAVRWTPRPEEAWQVHDGRTRRAFAPVPVLRLSSLPTLRDAVVAGAGAALLPRSL
VGGDVAAGRLACWGWLEGPPTELWALHTSRRLVSPKVNAFVAHLATALSTDLSANR
>Mature_296_residues
MDLSALADFNLVATHGGFGRAARASGRAKATLSRRVSELEASLGARLIERGERSLRLTEAGALLHARTGPLLSEIAEAGA
VVGGGLDRPRGRLRVSAPLLLSDTQLGRVAADFARAYPEVELEICAEDRFVDPIEEGFDVIIRVNPKPDERLVGRCVLRD
ELWLVAPPDLPRPDTSAASDVTPVPAAVRWTPRPEEAWQVHDGRTRRAFAPVPVLRLSSLPTLRDAVVAGAGAALLPRSL
VGGDVAAGRLACWGWLEGPPTELWALHTSRRLVSPKVNAFVAHLATALSTDLSANR

Specific function: Unknown

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786401, Length=300, Percent_Identity=29.3333333333333, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI145693193, Length=298, Percent_Identity=28.1879194630872, Blast_Score=95, Evalue=5e-21,
Organism=Escherichia coli, GI1787806, Length=270, Percent_Identity=27.7777777777778, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI87081978, Length=285, Percent_Identity=25.6140350877193, Blast_Score=81, Evalue=7e-17,
Organism=Escherichia coli, GI1787128, Length=305, Percent_Identity=24.9180327868852, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1787589, Length=255, Percent_Identity=27.4509803921569, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI1789639, Length=295, Percent_Identity=25.0847457627119, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1789440, Length=178, Percent_Identity=23.5955056179775, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI157672245, Length=166, Percent_Identity=30.1204819277108, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1787879, Length=308, Percent_Identity=27.9220779220779, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 31672; Mature: 31672

Theoretical pI: Translated: 8.10; Mature: 8.10

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
0.3 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLSALADFNLVATHGGFGRAARASGRAKATLSRRVSELEASLGARLIERGERSLRLTEA
CCCCHHCCCEEEEECCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHEEEECC
GALLHARTGPLLSEIAEAGAVVGGGLDRPRGRLRVSAPLLLSDTQLGRVAADFARAYPEV
CCEEEECCCHHHHHHHHHCCEECCCCCCCCCCEEEECCEEECCCHHHHHHHHHHHHCCCE
ELEICAEDRFVDPIEEGFDVIIRVNPKPDERLVGRCVLRDELWLVAPPDLPRPDTSAASD
EEEEECCCCCCCCHHCCCEEEEEECCCCCHHHHHHHEEECCEEEECCCCCCCCCCCCCCC
VTPVPAAVRWTPRPEEAWQVHDGRTRRAFAPVPVLRLSSLPTLRDAVVAGAGAALLPRSL
CCCCCCEEEECCCCCHHEECCCCCCCCEECCCCHHHHCCCCHHHHHHHHCCCHHHHHHHH
VGGDVAAGRLACWGWLEGPPTELWALHTSRRLVSPKVNAFVAHLATALSTDLSANR
HCCCCCCCCEEEEECCCCCCHHEEEECCCCHHCCCHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MDLSALADFNLVATHGGFGRAARASGRAKATLSRRVSELEASLGARLIERGERSLRLTEA
CCCCHHCCCEEEEECCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHEEEECC
GALLHARTGPLLSEIAEAGAVVGGGLDRPRGRLRVSAPLLLSDTQLGRVAADFARAYPEV
CCEEEECCCHHHHHHHHHCCEECCCCCCCCCCEEEECCEEECCCHHHHHHHHHHHHCCCE
ELEICAEDRFVDPIEEGFDVIIRVNPKPDERLVGRCVLRDELWLVAPPDLPRPDTSAASD
EEEEECCCCCCCCHHCCCEEEEEECCCCCHHHHHHHEEECCEEEECCCCCCCCCCCCCCC
VTPVPAAVRWTPRPEEAWQVHDGRTRRAFAPVPVLRLSSLPTLRDAVVAGAGAALLPRSL
CCCCCCEEEECCCCCHHEECCCCCCCCEECCCCHHHHCCCCHHHHHHHHCCCHHHHHHHH
VGGDVAAGRLACWGWLEGPPTELWALHTSRRLVSPKVNAFVAHLATALSTDLSANR
HCCCCCCCCEEEEECCCCCCHHEEEECCCCHHCCCHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]