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The map label for this gene is ysaA [H]

Identifier: 170683893

GI number: 170683893

Start: 3967180

End: 3967653

Strand: Reverse

Name: ysaA [H]

Synonym: EcSMS35_3896

Alternate gene names: 170683893

Gene position: 3967653-3967180 (Counterclockwise)

Preceding gene: 170680133

Following gene: 170683680

Centisome position: 78.28

GC content: 53.38

Gene sequence:

>474_bases
ATGAACCGGTTTATTATTGCGGATGCGACGAAATGTATCGGTTGCCGTACCTGTGAAGTGGCTTGCGCAGTGTCGCATCA
GGAGAATCAGGATTGCGCTGCGTTGTCACCAGACGAGTTTATTTCCCGTATTCGTGTCATTAAAGACCACAGCTGGACCA
CGGCAGTAGCCTGTCATCAGTGTGAAGATGCACCGTGCGCGAATGTCTGCCCTGTTGACGCGATAAGCCGTGAACATGGG
CATATTTTCGTTGAACAATCACGTTGCATTGGCTGTAAAAGCTGTATGCTGGCTTGCCCGTTTGGTGCGATGGAGGTCGT
TTCTTCGCGCAAAAAGGCGAGGGCGATTAAGTGCGATCTGTGCTGGCATCGGGCGACGGGACCGGCCTGTGTTGAAGCCT
GCCCGACAAAGGCGTTGCAGTGCATGGATGTCGAGAAAGTGCAGCGGCATCGGCTACGACAGCAGTCTGTTTGA

Upstream 100 bases:

>100_bases
GCTTTGGACAAGTGCCAAAACTTTAACATTTCCTTCGTTGGATCAAAGCAGTAGAGACGCGCTCTCTGGCACTCTGCTGT
TTTAGTGCAAAGGAGTGATC

Downstream 100 bases:

>100_bases
AACGTTTTTCCGGCGTTTTTTAACGCAGGAATAGTGCGCTTTGTTTATGCCGGATGCGGCTTAAACGCCTTATCCGGCCT
ACAAATTCTTCCAAATTCAA

Product: 4Fe-4S ferredoxin iron-sulfur binding domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 157; Mature: 157

Protein sequence:

>157_residues
MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRIRVIKDHSWTTAVACHQCEDAPCANVCPVDAISREHG
HIFVEQSRCIGCKSCMLACPFGAMEVVSSRKKARAIKCDLCWHRATGPACVEACPTKALQCMDVEKVQRHRLRQQSV

Sequences:

>Translated_157_residues
MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRIRVIKDHSWTTAVACHQCEDAPCANVCPVDAISREHG
HIFVEQSRCIGCKSCMLACPFGAMEVVSSRKKARAIKCDLCWHRATGPACVEACPTKALQCMDVEKVQRHRLRQQSV
>Mature_157_residues
MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRIRVIKDHSWTTAVACHQCEDAPCANVCPVDAISREHG
HIFVEQSRCIGCKSCMLACPFGAMEVVSSRKKARAIKCDLCWHRATGPACVEACPTKALQCMDVEKVQRHRLRQQSV

Specific function: Unknown

COG id: COG1142

COG function: function code C; Fe-S-cluster-containing hydrogenase components 2

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI2367245, Length=157, Percent_Identity=96.8152866242038, Blast_Score=308, Evalue=9e-86,
Organism=Escherichia coli, GI1789067, Length=162, Percent_Identity=61.7283950617284, Blast_Score=197, Evalue=2e-52,
Organism=Escherichia coli, GI1788811, Length=147, Percent_Identity=51.7006802721088, Blast_Score=145, Evalue=2e-36,
Organism=Escherichia coli, GI87082179, Length=159, Percent_Identity=45.2830188679245, Blast_Score=139, Evalue=7e-35,
Organism=Escherichia coli, GI87082180, Length=156, Percent_Identity=44.8717948717949, Blast_Score=136, Evalue=7e-34,
Organism=Escherichia coli, GI1789079, Length=189, Percent_Identity=38.6243386243386, Blast_Score=128, Evalue=2e-31,
Organism=Escherichia coli, GI87082114, Length=192, Percent_Identity=33.8541666666667, Blast_Score=106, Evalue=8e-25,
Organism=Escherichia coli, GI1787872, Length=165, Percent_Identity=33.9393939393939, Blast_Score=92, Evalue=2e-20,
Organism=Escherichia coli, GI1787122, Length=163, Percent_Identity=31.9018404907975, Blast_Score=92, Evalue=2e-20,
Organism=Escherichia coli, GI226510944, Length=144, Percent_Identity=36.8055555555556, Blast_Score=72, Evalue=2e-14,
Organism=Escherichia coli, GI1787963, Length=161, Percent_Identity=30.4347826086957, Blast_Score=71, Evalue=3e-14,
Organism=Escherichia coli, GI2367345, Length=156, Percent_Identity=31.4102564102564, Blast_Score=66, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900 [H]

Pfam domain/function: PF00037 Fer4 [H]

EC number: NA

Molecular weight: Translated: 17352; Mature: 17352

Theoretical pI: Translated: 7.83; Mature: 7.83

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

11.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
14.0 %Cys+Met (Translated Protein)
11.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
14.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRIRVIKDHSWTTAVACHQ
CCEEEEECCCHHHCCCCHHEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHC
CEDAPCANVCPVDAISREHGHIFVEQSRCIGCKSCMLACPFGAMEVVSSRKKARAIKCDL
CCCCCCCCCCCCHHCCCCCCEEEEECCCCCCHHHHHHHCCCCHHHHHHHHHHHHEEEEEE
CWHRATGPACVEACPTKALQCMDVEKVQRHRLRQQSV
EECCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRIRVIKDHSWTTAVACHQ
CCEEEEECCCHHHCCCCHHEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHC
CEDAPCANVCPVDAISREHGHIFVEQSRCIGCKSCMLACPFGAMEVVSSRKKARAIKCDL
CCCCCCCCCCCCHHCCCCCCEEEEECCCCCCHHHHHHHCCCCHHHHHHHHHHHHEEEEEE
CWHRATGPACVEACPTKALQCMDVEKVQRHRLRQQSV
EECCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8041620; 9278503 [H]