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The map label for this gene is yiaJ [H]

Identifier: 170680133

GI number: 170680133

Start: 3967755

End: 3968603

Strand: Reverse

Name: yiaJ [H]

Synonym: EcSMS35_3897

Alternate gene names: 170680133

Gene position: 3968603-3967755 (Counterclockwise)

Preceding gene: 170681568

Following gene: 170683893

Centisome position: 78.3

GC content: 52.3

Gene sequence:

>849_bases
ATGGGGAAAGAAGTGATGGGAAAAAAAGAGAACGAGATGGCGCAGGAAAAAGAGCGTCCAGCCGGAAGCCAGAGTCTGTT
TCGTGGGTTGATGCTGATTGAGATTTTGAGCAACTATCCAAACGGTTGTCCGTTGGCGCATCTTTCGGAACTGGCTGGTT
TAAATAAGAGTACCGTCCATCGCTTATTGCAGGGATTACAGTCCTGCGGCTATGTGACCACCGCGCCCGCCGCAGGGAGT
TACCGCCTGACCACCAAATTTATTGCCGTCGGGCAGAAGGCGCTGTCTTCGCTGAATATCATTCATATCGCCGCTCCGCA
TCTTGAGGCATTGAACATCGCCACTGGTGAAACCATTAACTTCTCCAGCCGCGAAGACGATCACGCTATTTTGATTTATA
AGCTGGAACCCACAACCGGGATGCTGCGAACCCGTGCCTATATAGGCCAGCATATGCCGCTCTACTGTTCCGCAATGGGC
AAGATCTACATGGCGTTTGGTCACCCGGACTACGTGAAGTCATACTGGGAAAGCCATCAGCATGAGATCCAGCCGTTAAC
CCGCAATACCATTACCGAGCTGCCCGCGATGTTCGACGAACTGGCGCACATTCGTGAAAGCGGAGCGGCGATGGACAGAG
AAGAAAACGAACTCGGCGTCTCCTGTATTGCTGTTCCGGTGTTTGATATTCATGGGCGGGTGCCGTACGCCGTGTCGATT
TCGCTTTCGACATCACGTCTGAAACAGGTGGGGGAGAAAAATCTCCTGAAACCACTGCGTGAAACCGCGCAGGCTATTTC
TAATGAACTGGGATTTACTGTCAGGGACGACCAGGGCGCGATCACATAA

Upstream 100 bases:

>100_bases
ATTGATGAATTTCAAAAAACTTAAAAATCAAGTGGTTATAAATTATCTGTCGAGATCGTGAACTACGGCACACTTTGCGC
TACCATCAGGACGCGACAAA

Downstream 100 bases:

>100_bases
CGCTTTGGACAAGTGCCAAAACTTTAACATTTCCTTCGTTGGATCAAAGCAGTAGAGACGCGCTCTCTGGCACTCTGCTG
TTTTAGTGCAAAGGAGTGAT

Product: IclR family transcriptional regulator

Products: NA

Alternate protein names: YiaKLMNOPQRS operon repressor; YiaK-S operon repressor [H]

Number of amino acids: Translated: 282; Mature: 281

Protein sequence:

>282_residues
MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCPLAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGS
YRLTTKFIAVGQKALSSLNIIHIAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
KIYMAFGHPDYVKSYWESHQHEIQPLTRNTITELPAMFDELAHIRESGAAMDREENELGVSCIAVPVFDIHGRVPYAVSI
SLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRDDQGAIT

Sequences:

>Translated_282_residues
MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCPLAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGS
YRLTTKFIAVGQKALSSLNIIHIAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
KIYMAFGHPDYVKSYWESHQHEIQPLTRNTITELPAMFDELAHIRESGAAMDREENELGVSCIAVPVFDIHGRVPYAVSI
SLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRDDQGAIT
>Mature_281_residues
GKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCPLAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSY
RLTTKFIAVGQKALSSLNIIHIAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMGK
IYMAFGHPDYVKSYWESHQHEIQPLTRNTITELPAMFDELAHIRESGAAMDREENELGVSCIAVPVFDIHGRVPYAVSIS
LSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRDDQGAIT

Specific function: Negatively controls the transcription of the yiaKLMNOPQRS operon, which may be involved in the utilization of 2,3-diketo-L-gulonate [H]

COG id: COG1414

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 iclR-ED (iclR effector binding) domain [H]

Homologues:

Organism=Escherichia coli, GI1789999, Length=282, Percent_Identity=99.645390070922, Blast_Score=583, Evalue=1e-168,
Organism=Escherichia coli, GI1786468, Length=251, Percent_Identity=33.4661354581673, Blast_Score=134, Evalue=9e-33,
Organism=Escherichia coli, GI1788131, Length=228, Percent_Identity=34.2105263157895, Blast_Score=131, Evalue=5e-32,
Organism=Escherichia coli, GI1786715, Length=251, Percent_Identity=29.8804780876494, Blast_Score=112, Evalue=2e-26,
Organism=Escherichia coli, GI87082359, Length=227, Percent_Identity=33.4801762114537, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI1790752, Length=219, Percent_Identity=30.5936073059361, Blast_Score=100, Evalue=9e-23,
Organism=Escherichia coli, GI1788581, Length=230, Percent_Identity=26.5217391304348, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI145693096, Length=143, Percent_Identity=32.1678321678322, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014757
- InterPro:   IPR005471
- InterPro:   IPR011991 [H]

Pfam domain/function: PF09339 HTH_IclR; PF01614 IclR [H]

EC number: NA

Molecular weight: Translated: 31082; Mature: 30951

Theoretical pI: Translated: 6.91; Mature: 6.91

Prosite motif: PS51077 HTH_ICLR ; PS51078 ICLR_ED

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCPLAHLSELAGLNKSTVH
CCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCHHHHH
RLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIHIAAPHLEALNIATGETIN
HHHHHHHHCCCEEECCCCCCCHHHHHHHHHHHHHHHHCCEEEEECCCHHEEECCCCCEEC
FSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMGKIYMAFGHPDYVKSYWESHQ
CCCCCCCCEEEEEEECCCHHHHHHHHHHCCCCCHHHHHHCCEEEEECCHHHHHHHHHHHH
HEIQPLTRNTITELPAMFDELAHIRESGAAMDREENELGVSCIAVPVFDIHGRVPYAVSI
HHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEEECCCCCCEEEEE
SLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRDDQGAIT
ECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCC
>Mature Secondary Structure 
GKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCPLAHLSELAGLNKSTVH
CCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCHHHHH
RLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIHIAAPHLEALNIATGETIN
HHHHHHHHCCCEEECCCCCCCHHHHHHHHHHHHHHHHCCEEEEECCCHHEEECCCCCEEC
FSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMGKIYMAFGHPDYVKSYWESHQ
CCCCCCCCEEEEEEECCCHHHHHHHHHHCCCCCHHHHHHCCEEEEECCHHHHHHHHHHHH
HEIQPLTRNTITELPAMFDELAHIRESGAAMDREENELGVSCIAVPVFDIHGRVPYAVSI
HHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEEECCCCCCEEEEE
SLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRDDQGAIT
ECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8041620; 9278503 [H]