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The map label for this gene is eefA [H]

Identifier: 170683115

GI number: 170683115

Start: 1859612

End: 1860733

Strand: Reverse

Name: eefA [H]

Synonym: EcSMS35_1836

Alternate gene names: 170683115

Gene position: 1860733-1859612 (Counterclockwise)

Preceding gene: 170681981

Following gene: 170683789

Centisome position: 36.71

GC content: 51.52

Gene sequence:

>1122_bases
ATGAAGTATATAGCAACATCTGTAGTGGCAATGCTGCTCTTATCGGGTTGCGATAATACGCAAAGTAACAATTCATCCCC
GTCAGAAACAGAAGTCGGCGTTGTTACACTCAAATCTCAACCGGTTTCGGTAGTCAGTGAATTAACCGGACGCACCAGTG
CTGCGCTCAGTGCCGAAGTACGTCCGCAGGTTGGGGGAATTATCCAGAAACGCTTATTTAAGGAAGGTGATCTGGTCAAG
GCTGGACAGCCGCTCTACCAGATTGATGCGGCCAGTTATCAGGCTGCATGGAATGAAGCCCGGGCAGCATTACAACAAGC
ACAGGCACTGGTAAAAGCCGATTGCCAGAAAGCGCAGCGTTATGCTCGACTGGTGAAAGAGAACGGTGTTTCACAACAGG
ATGCTGATGATGCTCAGTCTACCTGTGCACAAGATAAAGCCAGTGTAGAGGCGAAAAAAGCCGCACTGGAAACTGCGCGC
ATTAATCTTGACTGGACCACGGTAACCGCACCGATTTCAGGGCGCATTGGCATTTCGTCGGTAACCCCTGGCGCACTGGT
GACCGCGTCGCAAGATACAGCGTTAACGACGATTCGTGGTCTGGATACAATGTATGTCGACCTCACTCGCTCCAGTGTCG
ATTTATTACGTCTGCGTAAACAGTCACTGGCGACCAACAGTGACACCATGAGCGTCTCACTTATTCTGGAAGATGGCACA
ACCTACAGCGAAAAAGGGCGTCTGGAACTCACCGAAGTCGCGGTGGATGAGTCTACCGGTTCGGTGACATTACGGGCAAT
TTTCCCCAATCCACAACAGCAGTTATTACCGGGAATGTTTGTTCGCGCTCGTGTCGATGAAGGCGTGATGGAAGACGCTA
TTCTCGCGCCTCAACAGGGCGTCACGCGCGATGCTAAAGGCAATGCAACTGCGCTGGTGGTGAATAAAGACAATAAAGTA
GAGCAGCGAGCGCTCGAAACGGGAGAAACGTATGGTGATAAATGGCTGGTGCTGAACGGCCTGCACAACGGCGACCGACT
GATTGTTGAAGGTTCTGCCAAAGTCACTTCAGGGCAGACCGTCAAGGCTGTTGAAGTTCAGGCTAATGGAGGCAACGCCT
GA

Upstream 100 bases:

>100_bases
GCGAAATTCATTTCGCGATGCGGGTTCGTTATAACTGAATATCCCTGACATATCCTCATTAATTTCAATGAGGAGGGTAT
TTCTCACCCTGGAGAAAATA

Downstream 100 bases:

>100_bases
TGTTTTCGCGCTTTTTTGTTCGCCGCCCGGTCTTTGCCTGGGTTATCGCCATTTTGATTATGTTGGCGGGGATTCTGGCT
ATTCGCACATTACCCGTCGC

Product: multidrug efflux transport protein EefA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 373; Mature: 373

Protein sequence:

>373_residues
MKYIATSVVAMLLLSGCDNTQSNNSSPSETEVGVVTLKSQPVSVVSELTGRTSAALSAEVRPQVGGIIQKRLFKEGDLVK
AGQPLYQIDAASYQAAWNEARAALQQAQALVKADCQKAQRYARLVKENGVSQQDADDAQSTCAQDKASVEAKKAALETAR
INLDWTTVTAPISGRIGISSVTPGALVTASQDTALTTIRGLDTMYVDLTRSSVDLLRLRKQSLATNSDTMSVSLILEDGT
TYSEKGRLELTEVAVDESTGSVTLRAIFPNPQQQLLPGMFVRARVDEGVMEDAILAPQQGVTRDAKGNATALVVNKDNKV
EQRALETGETYGDKWLVLNGLHNGDRLIVEGSAKVTSGQTVKAVEVQANGGNA

Sequences:

>Translated_373_residues
MKYIATSVVAMLLLSGCDNTQSNNSSPSETEVGVVTLKSQPVSVVSELTGRTSAALSAEVRPQVGGIIQKRLFKEGDLVK
AGQPLYQIDAASYQAAWNEARAALQQAQALVKADCQKAQRYARLVKENGVSQQDADDAQSTCAQDKASVEAKKAALETAR
INLDWTTVTAPISGRIGISSVTPGALVTASQDTALTTIRGLDTMYVDLTRSSVDLLRLRKQSLATNSDTMSVSLILEDGT
TYSEKGRLELTEVAVDESTGSVTLRAIFPNPQQQLLPGMFVRARVDEGVMEDAILAPQQGVTRDAKGNATALVVNKDNKV
EQRALETGETYGDKWLVLNGLHNGDRLIVEGSAKVTSGQTVKAVEVQANGGNA
>Mature_373_residues
MKYIATSVVAMLLLSGCDNTQSNNSSPSETEVGVVTLKSQPVSVVSELTGRTSAALSAEVRPQVGGIIQKRLFKEGDLVK
AGQPLYQIDAASYQAAWNEARAALQQAQALVKADCQKAQRYARLVKENGVSQQDADDAQSTCAQDKASVEAKKAALETAR
INLDWTTVTAPISGRIGISSVTPGALVTASQDTALTTIRGLDTMYVDLTRSSVDLLRLRKQSLATNSDTMSVSLILEDGT
TYSEKGRLELTEVAVDESTGSVTLRAIFPNPQQQLLPGMFVRARVDEGVMEDAILAPQQGVTRDAKGNATALVVNKDNKV
EQRALETGETYGDKWLVLNGLHNGDRLIVEGSAKVTSGQTVKAVEVQANGGNA

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: COG0845

COG function: function code M; Membrane-fusion protein

Gene ontology:

Cell location: Cell inner membrane; Lipid-anchor. Note=The membrane anchor is not necessary for antibiotic efflux as the protein is functional when targeted to the periplasm by a foreign signal peptide [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1786668, Length=375, Percent_Identity=50.4, Blast_Score=333, Evalue=1e-92,
Organism=Escherichia coli, GI1789665, Length=360, Percent_Identity=49.1666666666667, Blast_Score=330, Evalue=9e-92,
Organism=Escherichia coli, GI1789929, Length=356, Percent_Identity=46.9101123595506, Blast_Score=304, Evalue=5e-84,
Organism=Escherichia coli, GI87082036, Length=325, Percent_Identity=28.3076923076923, Blast_Score=124, Evalue=1e-29,
Organism=Escherichia coli, GI87081796, Length=328, Percent_Identity=24.6951219512195, Blast_Score=65, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 39682; Mature: 39682

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYIATSVVAMLLLSGCDNTQSNNSSPSETEVGVVTLKSQPVSVVSELTGRTSAALSAEV
CCHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEECCCCHHHHHHHCCCCHHHEEECC
RPQVGGIIQKRLFKEGDLVKAGQPLYQIDAASYQAAWNEARAALQQAQALVKADCQKAQR
CCHHHHHHHHHHHCCCCEEECCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YARLVKENGVSQQDADDAQSTCAQDKASVEAKKAALETARINLDWTTVTAPISGRIGISS
HHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEEEEECCCCCCCCCC
VTPGALVTASQDTALTTIRGLDTMYVDLTRSSVDLLRLRKQSLATNSDTMSVSLILEDGT
CCCCEEEEECCCCCEEEECCCCEEEEEECCCHHHHHHHHHHHHCCCCCCEEEEEEEECCC
TYSEKGRLELTEVAVDESTGSVTLRAIFPNPQQQLLPGMFVRARVDEGVMEDAILAPQQG
CCCCCCCEEEEEEEEECCCCCEEEEEECCCCHHHHCCCCEEEECCCCCHHHHHHCCCCCC
VTRDAKGNATALVVNKDNKVEQRALETGETYGDKWLVLNGLHNGDRLIVEGSAKVTSGQT
CCCCCCCCEEEEEEECCCHHHHHHHHCCCCCCCCEEEEECCCCCCEEEEECCCEECCCCE
VKAVEVQANGGNA
EEEEEEECCCCCC
>Mature Secondary Structure
MKYIATSVVAMLLLSGCDNTQSNNSSPSETEVGVVTLKSQPVSVVSELTGRTSAALSAEV
CCHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEECCCCHHHHHHHCCCCHHHEEECC
RPQVGGIIQKRLFKEGDLVKAGQPLYQIDAASYQAAWNEARAALQQAQALVKADCQKAQR
CCHHHHHHHHHHHCCCCEEECCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YARLVKENGVSQQDADDAQSTCAQDKASVEAKKAALETARINLDWTTVTAPISGRIGISS
HHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEEEEECCCCCCCCCC
VTPGALVTASQDTALTTIRGLDTMYVDLTRSSVDLLRLRKQSLATNSDTMSVSLILEDGT
CCCCEEEEECCCCCEEEECCCCEEEEEECCCHHHHHHHHHHHHCCCCCCEEEEEEEECCC
TYSEKGRLELTEVAVDESTGSVTLRAIFPNPQQQLLPGMFVRARVDEGVMEDAILAPQQG
CCCCCCCEEEEEEEEECCCCCEEEEEECCCCHHHHCCCCEEEECCCCCHHHHHHCCCCCC
VTRDAKGNATALVVNKDNKVEQRALETGETYGDKWLVLNGLHNGDRLIVEGSAKVTSGQT
CCCCCCCCEEEEEEECCCHHHHHHHHCCCCCCCCEEEEECCCCCCEEEEECCCEECCCCE
VKAVEVQANGGNA
EEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7968531; 10984043; 9989496; 8226684; 8540696; 9603892; 10671490 [H]