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The map label for this gene is ycjT [H]

Identifier: 170682507

GI number: 170682507

Start: 1825698

End: 1827965

Strand: Reverse

Name: ycjT [H]

Synonym: EcSMS35_1806

Alternate gene names: 170682507

Gene position: 1827965-1825698 (Counterclockwise)

Preceding gene: 170684205

Following gene: 170682001

Centisome position: 36.07

GC content: 53.79

Gene sequence:

>2268_bases
ATGACCAGGCCAGTAACGTTAACGGAACCCCATTTCAGCCAGCATACCCTGAACAAGTATGCTTCGCTGATGGCGCAGGG
GAACGGCTATCTTGGGCTTCGCGCCAGCCATGAAGAAGATTATACGCGCCAGACACGCGGTATGTATCTGGCGGGGCTTT
ATCATCGCGCGGGGAAAAATGACATCAACGAACTGGTGAACCTGCCTGACGTCATAGGGATGGAGATTACCTTGAATGGT
GAACTTTTTGCGCTATCCCGCGAAACGTGGCAGCGCGAGCTTGACTTCGCCAGTGGAGAATTACGTCGCAATGTCGTCTG
GTCTTCAGCCAGCGGCGCACGTTATGCCATCGCCAGCCGTCGCTTTGTTTCGGCAGAACAACTGCCGCTGATGGCGCTGG
AAATCAGCATTACGCCGCTGGACGCTGACGCGTCAGTGCTGATTTCAACAGGCATTGACGCCACGCAAACCAACCACGGA
CGACAACATCTCGACGAAACCCAGGTGCGGGTGTTTGGTCAGCATTTGATGCAGGGGATCTATACCACTCAGGATGGACG
CAGTGATGTGGCCATCAGCTGTTGCTGCCAGGTGAGCGGTGACGTGCAGCAATGCTATACCGCCAAAGAGCGCCGCTTGC
TGCAACATACCAGTGCACAACTACCTGCGGGCAAAACGCTGACGCTGCAAAAACGGGTGTGGATCGACTGGCGGGACGAC
AGACACGTCGCTTTAGACGAGTGGGGTAGTGCATCGCTTCGTCAGCTTGAAATGTGTGTACAGCAGAGTTACGACCAACT
TCTTGCAGCATCCACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAACGGTGGCGATGCGCACGATC
AGCAAGCGTTAGATTATGCGCTTTATCACCTACGCATCATGACGCCTGCTCACGACGAGCGCAGCAGTATTGCGGCAAAA
GGTTTGACCGGGGAAGGCTACAAAGGCCACGTTTTCTGGGATACGGAAGTATTTTTACTGCCGTTCCATCTGTTTAGCGA
ACCGACGATTGCCAGAAGTTTACTGCGTTATCGCTGGCACAACTTGCCAGGCGCGCAGGAGAAAGCGCGACGCAACGGCT
GGCAGGGCGCGCTATTTCCGTGGGAAAGCGCGCGCAGCGGCGAAGAAGAGACGCCGGAATTTGCCGCCATTAACATTCGC
ACCGGGCTGCGGCAAAAAGTGGCCTCGGCGCAGGCGGAACATCATCTGGTCGCCGATATCGCCTGGGCGGTTGTTCAATA
CTGGCAGACCACGGGGGATGAAAGTTTCATTGCTCATGAAGGCATGGCGCTACTTCTGGAAACTGCAAAGTTCTGGATTA
GCCGCACGGTGAGGGTTAACGACCGTCTGGAAATTCATGATGTTATTGGGCCAGACGAATATACCGAACATGTCAATAAT
AACGCCTTCACCAGCTATATGGCGTATTACAACGTCCAGCAGGCGCTGAACATCGCTCGTCAATTTGGCTGTAGCGACGA
TGCGTTTATCCATCGCGCCGAAATGTTCCTTAAAGAACTGCGGCTGCCAGAAATTCAGCCCGACGGCGTTTTGCCGCAGG
ATGATTCGTTTATGGCGAAACCAGCGATTAATCTGGCGAAATACAAAGCGGCGGCGGGGAAGCAAACCATTCTGCTGGAT
TATTCACGCGCAGAAGTGAACGAGATGCAGATCCTCAAACAAGCTGATGTGGTGATGCTCAATTACATGCTGCCGGAGCA
GTACTCAGCGGCATCGTGTCTTGCCAATCTGCAATTTTATGAACCGCGCACCATTCACGACTCTTCACTGAGTAAAGCGA
TCCACGGCATTGTTGCCGCACGCTGTGGCCTGTTGGCGCAAAGTTATCAGTTCTGGCGGGAGGGGACTGAAATCGATCTT
GGTGCTGATCCGCATAGCTGTGATGACGGTATCCACGCTGCCGCAACTGGCGCAATCTGGTTGGGGGCGATTCAGGGTTT
TGCCGGGGCGAGCGTGCGCAACGGTGAATTACATCTCAATCCGGCGTTACCTGAGCAGTGGCAACAGTTGTCTTTCCCTC
TGTTCTGGCAGGGCTGTGAATTACAGGTCACTCTTGACGCGCAGCGCATTGCGATTCGGACTTCTGCGCCAGTTTCACTG
CGTTTGAACGGTCAGCTTATTTCAGTCTCTGAAGAATCTGTTTTCTGTTTAGGGGATTTTATTTTGCCCTTCAATGGGAC
CGCTACCACGCATCAGGAGGGTGAATGA

Upstream 100 bases:

>100_bases
GTAAGCCCGTGATGATAGCCGACGCCGAGCAGGGGTACATCATCCAGCAACTGGTGGCGGCGTTGTATCAATCCGCAGAA
ACAGGGACGCGTGTGGAATT

Downstream 100 bases:

>100_bases
AACTGCAAGGGGTAATTTTCGATCTGGATGGAGTAATCACCGATACCGCACATCTGCATTTCCAGGCGTGGCAGCAGATT
GCCGCTGAAATTGGCATCAG

Product: glycosyl hydrolase family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 755; Mature: 754

Protein sequence:

>755_residues
MTRPVTLTEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQTRGMYLAGLYHRAGKNDINELVNLPDVIGMEITLNG
ELFALSRETWQRELDFASGELRRNVVWSSASGARYAIASRRFVSAEQLPLMALEISITPLDADASVLISTGIDATQTNHG
RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCQVSGDVQQCYTAKERRLLQHTSAQLPAGKTLTLQKRVWIDWRDD
RHVALDEWGSASLRQLEMCVQQSYDQLLAASTENWRQWWQKRRITVNGGDAHDQQALDYALYHLRIMTPAHDERSSIAAK
GLTGEGYKGHVFWDTEVFLLPFHLFSEPTIARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETPEFAAINIR
TGLRQKVASAQAEHHLVADIAWAVVQYWQTTGDESFIAHEGMALLLETAKFWISRTVRVNDRLEIHDVIGPDEYTEHVNN
NAFTSYMAYYNVQQALNIARQFGCSDDAFIHRAEMFLKELRLPEIQPDGVLPQDDSFMAKPAINLAKYKAAAGKQTILLD
YSRAEVNEMQILKQADVVMLNYMLPEQYSAASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGLLAQSYQFWREGTEIDL
GADPHSCDDGIHAAATGAIWLGAIQGFAGASVRNGELHLNPALPEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSL
RLNGQLISVSEESVFCLGDFILPFNGTATTHQEGE

Sequences:

>Translated_755_residues
MTRPVTLTEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQTRGMYLAGLYHRAGKNDINELVNLPDVIGMEITLNG
ELFALSRETWQRELDFASGELRRNVVWSSASGARYAIASRRFVSAEQLPLMALEISITPLDADASVLISTGIDATQTNHG
RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCQVSGDVQQCYTAKERRLLQHTSAQLPAGKTLTLQKRVWIDWRDD
RHVALDEWGSASLRQLEMCVQQSYDQLLAASTENWRQWWQKRRITVNGGDAHDQQALDYALYHLRIMTPAHDERSSIAAK
GLTGEGYKGHVFWDTEVFLLPFHLFSEPTIARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETPEFAAINIR
TGLRQKVASAQAEHHLVADIAWAVVQYWQTTGDESFIAHEGMALLLETAKFWISRTVRVNDRLEIHDVIGPDEYTEHVNN
NAFTSYMAYYNVQQALNIARQFGCSDDAFIHRAEMFLKELRLPEIQPDGVLPQDDSFMAKPAINLAKYKAAAGKQTILLD
YSRAEVNEMQILKQADVVMLNYMLPEQYSAASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGLLAQSYQFWREGTEIDL
GADPHSCDDGIHAAATGAIWLGAIQGFAGASVRNGELHLNPALPEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSL
RLNGQLISVSEESVFCLGDFILPFNGTATTHQEGE
>Mature_754_residues
TRPVTLTEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQTRGMYLAGLYHRAGKNDINELVNLPDVIGMEITLNGE
LFALSRETWQRELDFASGELRRNVVWSSASGARYAIASRRFVSAEQLPLMALEISITPLDADASVLISTGIDATQTNHGR
QHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCQVSGDVQQCYTAKERRLLQHTSAQLPAGKTLTLQKRVWIDWRDDR
HVALDEWGSASLRQLEMCVQQSYDQLLAASTENWRQWWQKRRITVNGGDAHDQQALDYALYHLRIMTPAHDERSSIAAKG
LTGEGYKGHVFWDTEVFLLPFHLFSEPTIARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETPEFAAINIRT
GLRQKVASAQAEHHLVADIAWAVVQYWQTTGDESFIAHEGMALLLETAKFWISRTVRVNDRLEIHDVIGPDEYTEHVNNN
AFTSYMAYYNVQQALNIARQFGCSDDAFIHRAEMFLKELRLPEIQPDGVLPQDDSFMAKPAINLAKYKAAAGKQTILLDY
SRAEVNEMQILKQADVVMLNYMLPEQYSAASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGLLAQSYQFWREGTEIDLG
ADPHSCDDGIHAAATGAIWLGAIQGFAGASVRNGELHLNPALPEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLR
LNGQLISVSEESVFCLGDFILPFNGTATTHQEGE

Specific function: Unknown

COG id: COG1554

COG function: function code G; Trehalose and maltose hydrolases (possible phosphorylases)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 65 family [H]

Homologues:

Organism=Homo sapiens, GI187829418, Length=360, Percent_Identity=28.0555555555556, Blast_Score=107, Evalue=4e-23,
Organism=Escherichia coli, GI1787575, Length=755, Percent_Identity=93.9072847682119, Blast_Score=1493, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6325283, Length=396, Percent_Identity=22.7272727272727, Blast_Score=82, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24583760, Length=390, Percent_Identity=25.8974358974359, Blast_Score=105, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008928
- InterPro:   IPR012341
- InterPro:   IPR011013
- InterPro:   IPR005194
- InterPro:   IPR005195
- InterPro:   IPR005196
- InterPro:   IPR017045 [H]

Pfam domain/function: PF03633 Glyco_hydro_65C; PF03632 Glyco_hydro_65m; PF03636 Glyco_hydro_65N [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 84883; Mature: 84752

Theoretical pI: Translated: 5.88; Mature: 5.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRPVTLTEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQTRGMYLAGLYHRAGKN
CCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHCCEEEEHHHHHCCCH
DINELVNLPDVIGMEITLNGELFALSRETWQRELDFASGELRRNVVWSSASGARYAIASR
HHHHHHCCCCCEEEEEEECCEEEEEEHHHHHHHHCCCCCHHHHCEEEECCCCCEEEEHHC
RFVSAEQLPLMALEISITPLDADASVLISTGIDATQTNHGRQHLDETQVRVFGQHLMQGI
CCCCCCCCCEEEEEEEEEECCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
YTTQDGRSDVAISCCCQVSGDVQQCYTAKERRLLQHTSAQLPAGKTLTLQKRVWIDWRDD
EECCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEEEEECCC
RHVALDEWGSASLRQLEMCVQQSYDQLLAASTENWRQWWQKRRITVNGGDAHDQQALDYA
CEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCEEECCCCCCHHHHHHHH
LYHLRIMTPAHDERSSIAAKGLTGEGYKGHVFWDTEVFLLPFHLFSEPTIARSLLRYRWH
HHEEEEECCCCCCCHHHHCCCCCCCCEEEEEEECCEEEEEEHHHHCCCHHHHHHHHHHHC
NLPGAQEKARRNGWQGALFPWESARSGEEETPEFAAINIRTGLRQKVASAQAEHHLVADI
CCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHH
AWAVVQYWQTTGDESFIAHEGMALLLETAKFWISRTVRVNDRLEIHDVIGPDEYTEHVNN
HHHHHHHHHCCCCCCEEHHCCHHHHHHHHHHHHHEEEEECCCEEEEECCCCHHHHHHCCC
NAFTSYMAYYNVQQALNIARQFGCSDDAFIHRAEMFLKELRLPEIQPDGVLPQDDSFMAK
CHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
PAINLAKYKAAAGKQTILLDYSRAEVNEMQILKQADVVMLNYMLPEQYSAASCLANLQFY
CCHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHCCEEEEEECCCCCCCHHHHHHCCEEC
EPRTIHDSSLSKAIHGIVAARCGLLAQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIW
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCHHHHHHHHH
LGAIQGFAGASVRNGELHLNPALPEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSL
HHHHHCCCCCCEECCEEEECCCCCHHHHHCCCCEEECCCEEEEEECCEEEEEEECCCEEE
RLNGQLISVSEESVFCLGDFILPFNGTATTHQEGE
EECCEEEEECCCCEEEEEEEEEECCCCCCCCCCCC
>Mature Secondary Structure 
TRPVTLTEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQTRGMYLAGLYHRAGKN
CCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHCCEEEEHHHHHCCCH
DINELVNLPDVIGMEITLNGELFALSRETWQRELDFASGELRRNVVWSSASGARYAIASR
HHHHHHCCCCCEEEEEEECCEEEEEEHHHHHHHHCCCCCHHHHCEEEECCCCCEEEEHHC
RFVSAEQLPLMALEISITPLDADASVLISTGIDATQTNHGRQHLDETQVRVFGQHLMQGI
CCCCCCCCCEEEEEEEEEECCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
YTTQDGRSDVAISCCCQVSGDVQQCYTAKERRLLQHTSAQLPAGKTLTLQKRVWIDWRDD
EECCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEEEEECCC
RHVALDEWGSASLRQLEMCVQQSYDQLLAASTENWRQWWQKRRITVNGGDAHDQQALDYA
CEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCEEECCCCCCHHHHHHHH
LYHLRIMTPAHDERSSIAAKGLTGEGYKGHVFWDTEVFLLPFHLFSEPTIARSLLRYRWH
HHEEEEECCCCCCCHHHHCCCCCCCCEEEEEEECCEEEEEEHHHHCCCHHHHHHHHHHHC
NLPGAQEKARRNGWQGALFPWESARSGEEETPEFAAINIRTGLRQKVASAQAEHHLVADI
CCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHH
AWAVVQYWQTTGDESFIAHEGMALLLETAKFWISRTVRVNDRLEIHDVIGPDEYTEHVNN
HHHHHHHHHCCCCCCEEHHCCHHHHHHHHHHHHHEEEEECCCEEEEECCCCHHHHHHCCC
NAFTSYMAYYNVQQALNIARQFGCSDDAFIHRAEMFLKELRLPEIQPDGVLPQDDSFMAK
CHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
PAINLAKYKAAAGKQTILLDYSRAEVNEMQILKQADVVMLNYMLPEQYSAASCLANLQFY
CCHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHCCEEEEEECCCCCCCHHHHHHCCEEC
EPRTIHDSSLSKAIHGIVAARCGLLAQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIW
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCHHHHHHHHH
LGAIQGFAGASVRNGELHLNPALPEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSL
HHHHHCCCCCCEECCEEEECCCCCHHHHHCCCCEEECCCEEEEEECCEEEEEEECCCEEE
RLNGQLISVSEESVFCLGDFILPFNGTATTHQEGE
EECCEEEEECCCCEEEEEEEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]