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The map label for this gene is manA [H]

Identifier: 170682319

GI number: 170682319

Start: 1572738

End: 1573913

Strand: Reverse

Name: manA [H]

Synonym: EcSMS35_1586

Alternate gene names: 170682319

Gene position: 1573913-1572738 (Counterclockwise)

Preceding gene: 170680500

Following gene: 170682142

Centisome position: 31.05

GC content: 51.02

Gene sequence:

>1176_bases
ATGCAAAAACTCATTAACTCAGTGCAAAACTATGCCTGGGGCAGCAAAACGGCGTTGACTGAACTTTATGGTATGGAAAA
TCCGTCCAGCCAGCCGATGGCCGAGCTGTGGATGGGCGCACATCCGAAAAGCAGTTCACGAGTGCAGAATGCCGCCGGAG
ATATCGTTTCACTGCGTGATGTGATTGAGAGTGATAAAGCGACTCTTTTGGGAGATGCCGTTGCCAAACGCTTTGGCGAA
CTGCCTTTCCTGTTCAAAGTATTATGCGCAGCACAGCCGCTCTCCATTCAGGTTCATCCAAACAAACGCAATTCTGAAAT
CGGTTTTGCCAAAGAAAATGCCGCAGGTATTCCGATGGATGCCGCCGAGCGTAACTATAAAGATCCTAACCACAAGCCGG
AGCTGGTTTTTGCGCTGACGCCTTTCCTTGCGATGAACGCGTTTCGTGAGTTTTCCGAGATTGTCTCCCTGCTCCAGCCG
GTCGCAGGTGCACATCCGGCGATTGCTCACTTTTTACAACAGCCTGATGCCGAACGTTTAAGCGAACTGTTCGCCAGCCT
GTTGAATATGCAGGGTGAAGAAAAATCCCGCGCGCTGGCGATTTTAAAATCGGCCCTCGACAGCCAGCAGGGTGAACCGT
GGCAAACGATTCGTTTAATTTCTGAATTTTACCCGGATGACAGCGGTCTGTTCTCCCCGCTGTTGCTGAATGTGGTGAAA
TTGAATCCTGGCGAAGCGATGTTCCTGTTCGCTGAAACGCCGCACGCTTACCTGCAAGGCGTGGCGCTGGAAGTGATGGC
GAACTCCGATAACGTGCTGCGTGCGGGTCTGACGCCTAAATATATTGATATTCCGGAACTGGTTGCCAATGTGAAGTTCG
AAGCCAAACCGGCTAACCAGTTGTTGACCCAGCCGGTGAAACAAGGTGCAGAACTGGACTTCCCGATTCCTGTGGATGAT
TTTGCCTTCTCGCTGCATGACCTTAGTGATAAAGAAACCACCATTAGCCAGCAGAGTGCCGCCATTTTGTTCTGCGTCGA
AGGCGATGCAACGCTGTGTAAAGGTTCTCAGCAGTTACAGCTTAAACCGGGTGAATCAGCGTTTATTGCCGCCAACGAAT
CACCGGTGACTGTCAAAGGCCACGGTCGTTTAGCGCGTGTTTACAACAAGCTGTAA

Upstream 100 bases:

>100_bases
AAACTGAGACTAGTACGACTTTTTGCGGCTCCAGGTTACTTCCCGTAGGATTCTTGCTTTAATAGCGGGATTAATTTCCA
CATTAAAACAGGGATTGATC

Downstream 100 bases:

>100_bases
GAGCTTACTGAAAAAATTAACATCTCTTGCTAAGCTTATTAAAGGCTTATAACACCTTCAGGCGGCCAGTCCGCCTGATT
TCATTTTATGGATAATCATT

Product: mannose-6-phosphate isomerase, class I

Products: NA

Alternate protein names: Phosphohexomutase; Phosphomannose isomerase; PMI [H]

Number of amino acids: Translated: 391; Mature: 391

Protein sequence:

>391_residues
MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKSSSRVQNAAGDIVSLRDVIESDKATLLGDAVAKRFGE
LPFLFKVLCAAQPLSIQVHPNKRNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
VAGAHPAIAHFLQQPDAERLSELFASLLNMQGEEKSRALAILKSALDSQQGEPWQTIRLISEFYPDDSGLFSPLLLNVVK
LNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAGLTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDD
FAFSLHDLSDKETTISQQSAAILFCVEGDATLCKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL

Sequences:

>Translated_391_residues
MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKSSSRVQNAAGDIVSLRDVIESDKATLLGDAVAKRFGE
LPFLFKVLCAAQPLSIQVHPNKRNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
VAGAHPAIAHFLQQPDAERLSELFASLLNMQGEEKSRALAILKSALDSQQGEPWQTIRLISEFYPDDSGLFSPLLLNVVK
LNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAGLTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDD
FAFSLHDLSDKETTISQQSAAILFCVEGDATLCKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL
>Mature_391_residues
MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKSSSRVQNAAGDIVSLRDVIESDKATLLGDAVAKRFGE
LPFLFKVLCAAQPLSIQVHPNKRNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
VAGAHPAIAHFLQQPDAERLSELFASLLNMQGEEKSRALAILKSALDSQQGEPWQTIRLISEFYPDDSGLFSPLLLNVVK
LNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAGLTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDD
FAFSLHDLSDKETTISQQSAAILFCVEGDATLCKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL

Specific function: Involved in the conversion of glucose to GDP-L-fucose, which can be converted to L-fucose, a capsular polysaccharide [H]

COG id: COG1482

COG function: function code G; Phosphomannose isomerase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mannose-6-phosphate isomerase type 1 family [H]

Homologues:

Organism=Homo sapiens, GI4505235, Length=403, Percent_Identity=34.2431761786601, Blast_Score=187, Evalue=1e-47,
Organism=Escherichia coli, GI1787899, Length=391, Percent_Identity=98.7212276214834, Blast_Score=789, Evalue=0.0,
Organism=Caenorhabditis elegans, GI71997620, Length=394, Percent_Identity=31.2182741116751, Blast_Score=165, Evalue=3e-41,
Organism=Caenorhabditis elegans, GI17557650, Length=419, Percent_Identity=28.8782816229117, Blast_Score=131, Evalue=5e-31,
Organism=Saccharomyces cerevisiae, GI6320839, Length=419, Percent_Identity=31.7422434367542, Blast_Score=177, Evalue=3e-45,
Organism=Drosophila melanogaster, GI21356061, Length=400, Percent_Identity=31.5, Blast_Score=159, Evalue=3e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011051
- InterPro:   IPR001250
- InterPro:   IPR016305
- InterPro:   IPR018050
- InterPro:   IPR014710 [H]

Pfam domain/function: PF01238 PMI_typeI [H]

EC number: =5.3.1.8 [H]

Molecular weight: Translated: 42742; Mature: 42742

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: PS00965 PMI_I_1 ; PS00966 PMI_I_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKSSSRVQNAAGDIVSLRD
CHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCHHHHHCCCCCCCHHHHHHHHCCHHHHHH
VIESDKATLLGDAVAKRFGELPFLFKVLCAAQPLSIQVHPNKRNSEIGFAKENAAGIPMD
HHHCCCHHHHHHHHHHHHCCCHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCCCCCCC
AAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQPVAGAHPAIAHFLQQPDAERL
HHHCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHH
SELFASLLNMQGEEKSRALAILKSALDSQQGEPWQTIRLISEFYPDDSGLFSPLLLNVVK
HHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHE
LNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAGLTPKYIDIPELVANVKFEAKPANQ
ECCCCEEEEEECCCHHHHCCEEEEEEECCCCEEECCCCCCCCCHHHHHHCCCCCCCCHHH
LLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETTISQQSAAILFCVEGDATLCKGSQQLQ
HHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCHHHHCCCCCEEEEEECCCHHHHCCCCEEE
LKPGESAFIAANESPVTVKGHGRLARVYNKL
ECCCCCEEEEECCCCEEEECCCHHHHHHHCC
>Mature Secondary Structure
MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKSSSRVQNAAGDIVSLRD
CHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCHHHHHCCCCCCCHHHHHHHHCCHHHHHH
VIESDKATLLGDAVAKRFGELPFLFKVLCAAQPLSIQVHPNKRNSEIGFAKENAAGIPMD
HHHCCCHHHHHHHHHHHHCCCHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCCCCCCC
AAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQPVAGAHPAIAHFLQQPDAERL
HHHCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHH
SELFASLLNMQGEEKSRALAILKSALDSQQGEPWQTIRLISEFYPDDSGLFSPLLLNVVK
HHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHE
LNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAGLTPKYIDIPELVANVKFEAKPANQ
ECCCCEEEEEECCCHHHHCCEEEEEEECCCCEEECCCCCCCCCHHHHHHCCCCCCCCHHH
LLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETTISQQSAAILFCVEGDATLCKGSQQLQ
HHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCHHHHCCCCCEEEEEECCCHHHHCCCCEEE
LKPGESAFIAANESPVTVKGHGRLARVYNKL
ECCCCCEEEEECCCCEEEECCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6397402; 9097039; 9278503 [H]