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The map label for this gene is dhaR [H]

Identifier: 170682289

GI number: 170682289

Start: 1960779

End: 1962698

Strand: Reverse

Name: dhaR [H]

Synonym: EcSMS35_1940

Alternate gene names: 170682289

Gene position: 1962698-1960779 (Counterclockwise)

Preceding gene: 170683395

Following gene: 170683871

Centisome position: 38.72

GC content: 51.41

Gene sequence:

>1920_bases
ATGAGTGGCGCTTTTAACAACGATGGTCGGGACATATCTCCCTTAATTGCAACCTCCTGGGAGCGATGCATTAAGCTGAT
GAAACGGGAGACATGGAACGTACCACACCAGGCCCAGGGCGTGACATTTGCTTCTATTTATCGGCGTAAGAAAGCGATGC
TGACGCTCGGGCAGGCTGCGCTGGAAGATGCCTGGGAATATATGGCACCGCGAGAGTGTGCGTTGCTTATCCTCGATGAA
ACCGCCTGCATTCTCAGCCGTAATGGCGATCCGCAAACCTTGCAGCAGCTAAGTGCACTGGGATTCAATGACGGCACGTA
TTGCGCCGAGGGAATTATTGGTACTTGTGCGCTATCATTAGCGGCTATCTCTGGTCAGGCCGTGAAAACGATGGCCGATC
AACATTTCAAACAGGCACTCTGGAACTGGGCCTTTTGTGCAACGCCGTTGTTTGACAGCAAGGGCCGATTGACGGGAACA
ATAGCGCTGGCGTGTCCGGTTGAACAAACTACCGCAGCTGATTTGCCGTTGACGTTGGCAATCGCACGCGAGGTCGGAAA
TTTACTGCTGACGGACAGTTTGCTCGCTGAAACTAACCGTCATTTAAATCAACTTAATGCCCTGTTAGAAAGTATGGATG
ATGGCGTGATTAGCTGGGACGAGCAGGGTAATTTGCAATTTATCAATGCCCAGGCGGCGCGGGTCTTGCGCCTTGACGCG
ACGGCAAGTCAGGGAAGGGCGATCACTGAACTCTTAACGTTACCCGCCGTATTGCAACAAGCAATAAAACAGGCACATCC
GCTCAAACACGTAGAAGCAACCTTTGAAAGTCAGCACCAGTTTATTGATGCGGTGATAACCCTTAAACCGATAATAGAAA
CGCAGGGAACCAGCTTTATTTTGTTGCTCCATCCTGTGGAACAGATGCGGCAGTTAATGACCAGTCAATTAGGAAAAGTC
AGCCATACTTTCGCTCATATGCCACAGGACGATCCGCAAACCCGCCGCTTGATTCATTTTGGTCGCCAGGCGGCGCGCAG
TAGCCTTCCTGTCCTGCTTTGTGGAGAAGAGGGCGTGGGCAAGGCACTGCTAAGTCAGGCAATTCATAATGAAAGTGAGC
GTGCAGCGGGGCCTTATATCGCCGTCAATTGTGAGTTATATGGTGATGCGGCGCTGGCGGAAGAATTTATTGGCGGCGAT
CGCACGGACAATGAAAATGGTCGTCTGAGTCGGCTGGAACTGGCGCACGGCGGCACGCTGTTTCTTGAAAAGATTGAATA
TCTGGCGGTGGAGTTACAGTCTGCTTTGCTTCAGGTTATCAAGCAGGGGGTTATCACGCGACTGGATGCGCGGCGTTTAA
TACCCATTGATGTCAAAGTGATTGCCACAACGACCGCGGACCTCGCAATGCTGGTGGAACAAAATCGTTTTAGTCGCCAG
CTGTATTACGCGCTGCATGCATTTGAAATTACCATCCCGCCACTGCGTATGCGGCGTGGCAGCATTCCGGCGCTGGTGAA
TAACAAATTACGCAGCCTTGAAAAACGCTTCTCTACGCGGCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGTTTCTT
GTGCATGGCCAGGCAACGATTTTGAACTTTACAGCGTCATCGAGAATCTTGCTCTGAGTAGTGATAACGGGCGCATTCGC
GTCAGTGATTTACCTGAACATCTGTTTACCGAGCAGGCGACAGATGATGTCAGCGCCACTCGCCTTACCACCAGTCTGTC
ATTTGCGGAAGTTGAAAAAGAGGCAATTATTAACGCAGCCCAGGTCACAGGCGGTCGCATTCAGGAAATGTCGGCTTTAC
TTGGAATCGGCCGCACTACGCTGTGGCGGAAAATGAAGCAACATGGCATTGATGCAGGGCAGTTTAAGCGCCGTGGATAG

Upstream 100 bases:

>100_bases
GAAACATATTCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTT
TGTCATAAGGGATGCGGGAT

Downstream 100 bases:

>100_bases
CGGGAGAGTATGTACTTTCCCGCTATGAATTGCTAATTAATAGCCACCATGACTGCCGTGATTTGGTCCACCATTTGAAG
GTGGCATTGCCTTATCGGAG

Product: DNA-binding transcriptional regulator DhaR

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 639; Mature: 638

Protein sequence:

>639_residues
MSGAFNNDGRDISPLIATSWERCIKLMKRETWNVPHQAQGVTFASIYRRKKAMLTLGQAALEDAWEYMAPRECALLILDE
TACILSRNGDPQTLQQLSALGFNDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRLTGT
IALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVISWDEQGNLQFINAQAARVLRLDA
TASQGRAITELLTLPAVLQQAIKQAHPLKHVEATFESQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKV
SHTFAHMPQDDPQTRRLIHFGRQAARSSLPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGDAALAEEFIGGD
RTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITRLDARRLIPIDVKVIATTTADLAMLVEQNRFSRQ
LYYALHAFEITIPPLRMRRGSIPALVNNKLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIR
VSDLPEHLFTEQATDDVSATRLTTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTLWRKMKQHGIDAGQFKRRG

Sequences:

>Translated_639_residues
MSGAFNNDGRDISPLIATSWERCIKLMKRETWNVPHQAQGVTFASIYRRKKAMLTLGQAALEDAWEYMAPRECALLILDE
TACILSRNGDPQTLQQLSALGFNDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRLTGT
IALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVISWDEQGNLQFINAQAARVLRLDA
TASQGRAITELLTLPAVLQQAIKQAHPLKHVEATFESQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKV
SHTFAHMPQDDPQTRRLIHFGRQAARSSLPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGDAALAEEFIGGD
RTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITRLDARRLIPIDVKVIATTTADLAMLVEQNRFSRQ
LYYALHAFEITIPPLRMRRGSIPALVNNKLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIR
VSDLPEHLFTEQATDDVSATRLTTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTLWRKMKQHGIDAGQFKRRG
>Mature_638_residues
SGAFNNDGRDISPLIATSWERCIKLMKRETWNVPHQAQGVTFASIYRRKKAMLTLGQAALEDAWEYMAPRECALLILDET
ACILSRNGDPQTLQQLSALGFNDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRLTGTI
ALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVISWDEQGNLQFINAQAARVLRLDAT
ASQGRAITELLTLPAVLQQAIKQAHPLKHVEATFESQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVS
HTFAHMPQDDPQTRRLIHFGRQAARSSLPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGDAALAEEFIGGDR
TDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITRLDARRLIPIDVKVIATTTADLAMLVEQNRFSRQL
YYALHAFEITIPPLRMRRGSIPALVNNKLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRV
SDLPEHLFTEQATDDVSATRLTTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTLWRKMKQHGIDAGQFKRRG

Specific function: Positively regulates the dhaKLM operon from a sigma-70 promoter. Represses its own synthesis [H]

COG id: COG3284

COG function: function code QK; Transcriptional activator of acetoin/glycerol metabolism

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI87081858, Length=638, Percent_Identity=99.0595611285266, Blast_Score=1300, Evalue=0.0,
Organism=Escherichia coli, GI87082117, Length=306, Percent_Identity=34.9673202614379, Blast_Score=154, Evalue=1e-38,
Organism=Escherichia coli, GI1788905, Length=310, Percent_Identity=32.258064516129, Blast_Score=150, Evalue=4e-37,
Organism=Escherichia coli, GI1790299, Length=312, Percent_Identity=32.0512820512821, Blast_Score=149, Evalue=7e-37,
Organism=Escherichia coli, GI1788550, Length=325, Percent_Identity=31.0769230769231, Blast_Score=149, Evalue=7e-37,
Organism=Escherichia coli, GI1789233, Length=450, Percent_Identity=28.6666666666667, Blast_Score=144, Evalue=2e-35,
Organism=Escherichia coli, GI1789087, Length=326, Percent_Identity=31.5950920245399, Blast_Score=139, Evalue=5e-34,
Organism=Escherichia coli, GI1790437, Length=287, Percent_Identity=33.1010452961672, Blast_Score=138, Evalue=9e-34,
Organism=Escherichia coli, GI1786524, Length=303, Percent_Identity=33.3333333333333, Blast_Score=134, Evalue=3e-32,
Organism=Escherichia coli, GI1787583, Length=337, Percent_Identity=26.4094955489614, Blast_Score=115, Evalue=1e-26,
Organism=Escherichia coli, GI87082152, Length=207, Percent_Identity=34.7826086956522, Blast_Score=108, Evalue=1e-24,
Organism=Escherichia coli, GI87081872, Length=295, Percent_Identity=27.7966101694915, Blast_Score=103, Evalue=3e-23,
Organism=Escherichia coli, GI1789828, Length=242, Percent_Identity=29.7520661157025, Blast_Score=93, Evalue=6e-20,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR003018
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR002078 [H]

Pfam domain/function: PF01590 GAF; PF02954 HTH_8; PF00989 PAS; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 70496; Mature: 70365

Theoretical pI: Translated: 6.46; Mature: 6.46

Prosite motif: PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGAFNNDGRDISPLIATSWERCIKLMKRETWNVPHQAQGVTFASIYRRKKAMLTLGQAA
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
LEDAWEYMAPRECALLILDETACILSRNGDPQTLQQLSALGFNDGTYCAEGIIGTCALSL
HHHHHHHCCCCCEEEEEEECCEEEEECCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
AAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRLTGTIALACPVEQTTAADLPLTLA
HHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEECCCCCCCCCCCHHHH
IAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVISWDEQGNLQFINAQAARVLRLDA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCEEEEEHHHEEEEEEEC
TASQGRAITELLTLPAVLQQAIKQAHPLKHVEATFESQHQFIDAVITLKPIIETQGTSFI
CCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEE
LLLHPVEQMRQLMTSQLGKVSHTFAHMPQDDPQTRRLIHFGRQAARSSLPVLLCGEEGVG
EEECCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCEEEECCCCCC
KALLSQAIHNESERAAGPYIAVNCELYGDAALAEEFIGGDRTDNENGRLSRLELAHGGTL
HHHHHHHHHCCCHHCCCCEEEEEEEECCCHHHHHHHHCCCCCCCCCCCEEEEEECCCCHH
FLEKIEYLAVELQSALLQVIKQGVITRLDARRLIPIDVKVIATTTADLAMLVEQNRFSRQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEECHHHHHHHHHHHHHHHH
LYYALHAFEITIPPLRMRRGSIPALVNNKLRSLEKRFSTRLKIDDDALARLVSCAWPGND
HHHHHHHEEEECCHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCC
FELYSVIENLALSSDNGRIRVSDLPEHLFTEQATDDVSATRLTTSLSFAEVEKEAIINAA
HHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
QVTGGRIQEMSALLGIGRTTLWRKMKQHGIDAGQFKRRG
HHCCCHHHHHHHHHHCCHHHHHHHHHHHCCCHHHHCCCC
>Mature Secondary Structure 
SGAFNNDGRDISPLIATSWERCIKLMKRETWNVPHQAQGVTFASIYRRKKAMLTLGQAA
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
LEDAWEYMAPRECALLILDETACILSRNGDPQTLQQLSALGFNDGTYCAEGIIGTCALSL
HHHHHHHCCCCCEEEEEEECCEEEEECCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
AAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRLTGTIALACPVEQTTAADLPLTLA
HHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEECCCCCCCCCCCHHHH
IAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVISWDEQGNLQFINAQAARVLRLDA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCEEEEEHHHEEEEEEEC
TASQGRAITELLTLPAVLQQAIKQAHPLKHVEATFESQHQFIDAVITLKPIIETQGTSFI
CCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEE
LLLHPVEQMRQLMTSQLGKVSHTFAHMPQDDPQTRRLIHFGRQAARSSLPVLLCGEEGVG
EEECCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCEEEECCCCCC
KALLSQAIHNESERAAGPYIAVNCELYGDAALAEEFIGGDRTDNENGRLSRLELAHGGTL
HHHHHHHHHCCCHHCCCCEEEEEEEECCCHHHHHHHHCCCCCCCCCCCEEEEEECCCCHH
FLEKIEYLAVELQSALLQVIKQGVITRLDARRLIPIDVKVIATTTADLAMLVEQNRFSRQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEECHHHHHHHHHHHHHHHH
LYYALHAFEITIPPLRMRRGSIPALVNNKLRSLEKRFSTRLKIDDDALARLVSCAWPGND
HHHHHHHEEEECCHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCC
FELYSVIENLALSSDNGRIRVSDLPEHLFTEQATDDVSATRLTTSLSFAEVEKEAIINAA
HHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
QVTGGRIQEMSALLGIGRTTLWRKMKQHGIDAGQFKRRG
HHCCCHHHHHHHHHHCCHHHHHHHHHHHCCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]