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The map label for this gene is ynfE [H]

Identifier: 170682222

GI number: 170682222

Start: 1603333

End: 1605759

Strand: Reverse

Name: ynfE [H]

Synonym: EcSMS35_1613

Alternate gene names: 170682222

Gene position: 1605759-1603333 (Counterclockwise)

Preceding gene: 170681546

Following gene: 170679927

Centisome position: 31.68

GC content: 50.06

Gene sequence:

>2427_bases
ATGTCCAAAAATGAACAAATGGTGGGCATCAGCCGCAGAACACTCGTTAAATCTACCGCGATAGGTTCTCTGGCGCTGGC
TGCAGGCGGTTTTTCTTTACCGTTCACCCTGCGCAGTTCAGCGGCAGCGGTACAACAGGCCAGCGAAAAAGTGGTCTGGG
GTGCCTGTTCCGTCAACTGTGGTAGCCGCTGTGCACTTCGTCTACATGTTAAAGATAATGAAGTCACTTGGGTGGAAACT
GACAACACCGGCAACGACGAGTATGGCAATCAACAAGTACGCGCCTGTTTGCGCGGTCGGTCCATCCGTCGACGTATTAA
TCATCCCGATCGCTTGAATTACCCAATGAAACGCGTGGGCAAACGTGGCGAAGGCAAATTCGAACGGATTAGCTGGGATG
AAGCCCTGGATACTATCGCCAGTAGCCTGAAAAAAACCGTCGAACAATATGGCAATGAGGCTGTATATATTCAGTACTCT
TCAGGGATCGTTGGCGGCAATATGACCCGCTCTTCACCATCAGCCTCGGCGGTCAAACGCCTGATGAACTGCTACGGCGG
TTCACTCAATCAGTATGGCTCCTACAGCACCGCGCAAATTTCCTGTGCCATGCCCTACACCTACGGCAGTAATGATGGCA
ACAGCACCACGGATATTGAAAACAGCAAGCTGGTCGTGATGTTTGGTAACAACCCGGCAGAAACCCGCATGAGCGGCGGT
GGCATCACTTATCTTCTTGAAAAAGCACGCGAGAAATCGAACGCAAAAATGATTGTTATCGATCCGCGATATACCGATAC
GGCTGCTGGTCGTGAAGACGAATGGTTACCTATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGGGTATTGCCTGGGTAT
TGATTAACGAAAATCTCGTTGATCAACCTTTTCTCGATAAATACTGCGTCGGCTATGACGAAAAAACCTTACCCGCAGAT
GCACCAAAAAATGGTCACTATAAAGCCTATATTCTTGGTGAAGGTGATGATAAAACAGCGAAAACGCCGCAGTGGGCTTC
GCAAATTACCGGTATCCCGGTGGACCGTATCATCAAGCTGGCTCGTGAAATTGGCACAGCAAAACCCGCTTATATCTGCC
AGGGCTGGGGGCCGCAACGCCAGGCAAACGGTGAGCTGACCGCACGCGCTATTGCTATGTTGCCTATTTTGACGGGCAAT
GTCGGCATCAGCGGCGGAAATAGTGGTGCGCGTGAATCGACCTATACCATTACCATAGAACGCCTACCGGTGCTGGATAA
TCCGGTCAAAACGTCAATCTCTTGCTTCAGCTGGACGGATGCTATCGATCATGGCCCGCAAATGACGGCAATCCGCGACG
GTGTCCGGGGCAAAGATAAACTGGATGTGCCCATTAAGTTCATCTGGAACTACGCGGGAAATACCCTCGTTAATCAGCAT
TCTGACATCAACAAAACGCATGAAATTCTGCAGGACGAATCGAAATGCGAAATGATTGTGGTCATCGAAAACTTTATGAC
CTCATCGGCAAAATATGCCGACATTCTGCTGCCAGACCTGATGACCGTTGAGCAGGAAGATATTATTCCTAACGACTACG
CCGGTAACATGGGATATCTCATTTTCCTTCAGCCAGTCACCAGCGAAAAATTCGAACGCAAACCGATTTACTGGATCCTG
AGTGAAGTCGCGAAACGTCTTGGACCTGACGTCTATCAAAAGTTCACTGAAGGTCGCACTCAGGAACAATGGTTACAACA
TCTGTACGCCAAAATGCTTGCCAAAGATCCGGCGTTACCGTCTTACGACGAACTGAAAAAAATGGGTATCTATAAGCGTA
AAGATCCCAATGGTCATTTTGTCGCCTACAAAGCATTTCGTGAGGACCCCGAGGCAAATCCACTTAAAACGCCTTCCGGT
AAGATTGAAATTTATTCCAACAGGCTGGCGGAAATTGCCCGTACCTGGGAACTGGAAAAAGATGAAGTGATAAGCCCATT
GCCAGTTTATGCCTCAACCTTTGAAGGCTGGGACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATT
ACAAATCCCGTACTCACTCGACCTACGGCAACATTGATGTCCTGAAGGCAGCCTGCCGTCAGGAGGTGTGGATCAACCCT
ATAGATGCACAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGC
CAAAGTGACACCACGCATTCTCCCTGGAGTTAGTGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATA
AAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACT
AATCTGGTCGAGATCGAAAAAATATAA

Upstream 100 bases:

>100_bases
ATAATTCTTAACCCAAAATGGGTAGACTCCCTCTATTGTTAGCGCGCTAAATATTCAATATATAAACTTTTATATAACGA
TAAAGAACAGGGAGTGAGTT

Downstream 100 bases:

>100_bases
CCCTCTACGACCATAAATTTTGACATGACATTTTGTTTGAAAAGCAATAAGTGAGTAATGATGAAAATCCATACCACAGA
GGCGCTCATGAAGGCTGAAA

Product: anaerobic dimethyl sulfoxide reductase, A subunit YnfE

Products: NA

Alternate protein names: DMSO reductase [H]

Number of amino acids: Translated: 808; Mature: 807

Protein sequence:

>808_residues
MSKNEQMVGISRRTLVKSTAIGSLALAAGGFSLPFTLRSSAAAVQQASEKVVWGACSVNCGSRCALRLHVKDNEVTWVET
DNTGNDEYGNQQVRACLRGRSIRRRINHPDRLNYPMKRVGKRGEGKFERISWDEALDTIASSLKKTVEQYGNEAVYIQYS
SGIVGGNMTRSSPSASAVKRLMNCYGGSLNQYGSYSTAQISCAMPYTYGSNDGNSTTDIENSKLVVMFGNNPAETRMSGG
GITYLLEKAREKSNAKMIVIDPRYTDTAAGREDEWLPIRPGTDAALVAGIAWVLINENLVDQPFLDKYCVGYDEKTLPAD
APKNGHYKAYILGEGDDKTAKTPQWASQITGIPVDRIIKLAREIGTAKPAYICQGWGPQRQANGELTARAIAMLPILTGN
VGISGGNSGARESTYTITIERLPVLDNPVKTSISCFSWTDAIDHGPQMTAIRDGVRGKDKLDVPIKFIWNYAGNTLVNQH
SDINKTHEILQDESKCEMIVVIENFMTSSAKYADILLPDLMTVEQEDIIPNDYAGNMGYLIFLQPVTSEKFERKPIYWIL
SEVAKRLGPDVYQKFTEGRTQEQWLQHLYAKMLAKDPALPSYDELKKMGIYKRKDPNGHFVAYKAFREDPEANPLKTPSG
KIEIYSNRLAEIARTWELEKDEVISPLPVYASTFEGWDSPERRTFPLQLFGFHYKSRTHSTYGNIDVLKAACRQEVWINP
IDAQKRGIANGDMVRVFNHRGEVRLPAKVTPRILPGVSAMGQGAWHEANMSGDKIDHGGCVNTLTTLRPSPLAKGNPQHT
NLVEIEKI

Sequences:

>Translated_808_residues
MSKNEQMVGISRRTLVKSTAIGSLALAAGGFSLPFTLRSSAAAVQQASEKVVWGACSVNCGSRCALRLHVKDNEVTWVET
DNTGNDEYGNQQVRACLRGRSIRRRINHPDRLNYPMKRVGKRGEGKFERISWDEALDTIASSLKKTVEQYGNEAVYIQYS
SGIVGGNMTRSSPSASAVKRLMNCYGGSLNQYGSYSTAQISCAMPYTYGSNDGNSTTDIENSKLVVMFGNNPAETRMSGG
GITYLLEKAREKSNAKMIVIDPRYTDTAAGREDEWLPIRPGTDAALVAGIAWVLINENLVDQPFLDKYCVGYDEKTLPAD
APKNGHYKAYILGEGDDKTAKTPQWASQITGIPVDRIIKLAREIGTAKPAYICQGWGPQRQANGELTARAIAMLPILTGN
VGISGGNSGARESTYTITIERLPVLDNPVKTSISCFSWTDAIDHGPQMTAIRDGVRGKDKLDVPIKFIWNYAGNTLVNQH
SDINKTHEILQDESKCEMIVVIENFMTSSAKYADILLPDLMTVEQEDIIPNDYAGNMGYLIFLQPVTSEKFERKPIYWIL
SEVAKRLGPDVYQKFTEGRTQEQWLQHLYAKMLAKDPALPSYDELKKMGIYKRKDPNGHFVAYKAFREDPEANPLKTPSG
KIEIYSNRLAEIARTWELEKDEVISPLPVYASTFEGWDSPERRTFPLQLFGFHYKSRTHSTYGNIDVLKAACRQEVWINP
IDAQKRGIANGDMVRVFNHRGEVRLPAKVTPRILPGVSAMGQGAWHEANMSGDKIDHGGCVNTLTTLRPSPLAKGNPQHT
NLVEIEKI
>Mature_807_residues
SKNEQMVGISRRTLVKSTAIGSLALAAGGFSLPFTLRSSAAAVQQASEKVVWGACSVNCGSRCALRLHVKDNEVTWVETD
NTGNDEYGNQQVRACLRGRSIRRRINHPDRLNYPMKRVGKRGEGKFERISWDEALDTIASSLKKTVEQYGNEAVYIQYSS
GIVGGNMTRSSPSASAVKRLMNCYGGSLNQYGSYSTAQISCAMPYTYGSNDGNSTTDIENSKLVVMFGNNPAETRMSGGG
ITYLLEKAREKSNAKMIVIDPRYTDTAAGREDEWLPIRPGTDAALVAGIAWVLINENLVDQPFLDKYCVGYDEKTLPADA
PKNGHYKAYILGEGDDKTAKTPQWASQITGIPVDRIIKLAREIGTAKPAYICQGWGPQRQANGELTARAIAMLPILTGNV
GISGGNSGARESTYTITIERLPVLDNPVKTSISCFSWTDAIDHGPQMTAIRDGVRGKDKLDVPIKFIWNYAGNTLVNQHS
DINKTHEILQDESKCEMIVVIENFMTSSAKYADILLPDLMTVEQEDIIPNDYAGNMGYLIFLQPVTSEKFERKPIYWILS
EVAKRLGPDVYQKFTEGRTQEQWLQHLYAKMLAKDPALPSYDELKKMGIYKRKDPNGHFVAYKAFREDPEANPLKTPSGK
IEIYSNRLAEIARTWELEKDEVISPLPVYASTFEGWDSPERRTFPLQLFGFHYKSRTHSTYGNIDVLKAACRQEVWINPI
DAQKRGIANGDMVRVFNHRGEVRLPAKVTPRILPGVSAMGQGAWHEANMSGDKIDHGGCVNTLTTLRPSPLAKGNPQHTN
LVEIEKI

Specific function: Terminal reductase during anaerobic growth on various sulfoxide and N-oxide compounds [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic molybdopterin-containing oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI1787870, Length=808, Percent_Identity=98.6386138613861, Blast_Score=1665, Evalue=0.0,
Organism=Escherichia coli, GI171474008, Length=801, Percent_Identity=70.9113607990012, Blast_Score=1231, Evalue=0.0,
Organism=Escherichia coli, GI87081797, Length=805, Percent_Identity=67.3291925465839, Blast_Score=1138, Evalue=0.0,
Organism=Escherichia coli, GI145693196, Length=753, Percent_Identity=30.5444887118194, Blast_Score=289, Evalue=5e-79,
Organism=Escherichia coli, GI1787231, Length=760, Percent_Identity=29.7368421052632, Blast_Score=267, Evalue=2e-72,
Organism=Escherichia coli, GI87081994, Length=848, Percent_Identity=28.6556603773585, Blast_Score=262, Evalue=5e-71,
Organism=Escherichia coli, GI3868721, Length=787, Percent_Identity=24.2693773824651, Blast_Score=134, Evalue=2e-32,
Organism=Escherichia coli, GI3868720, Length=455, Percent_Identity=25.7142857142857, Blast_Score=99, Evalue=1e-21,
Organism=Escherichia coli, GI3868719, Length=457, Percent_Identity=24.72647702407, Blast_Score=86, Evalue=1e-17,
Organism=Escherichia coli, GI1787741, Length=427, Percent_Identity=25.7611241217799, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI1788534, Length=890, Percent_Identity=22.0224719101124, Blast_Score=80, Evalue=5e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011888
- InterPro:   IPR009010
- InterPro:   IPR006657
- InterPro:   IPR006656
- InterPro:   IPR006963
- InterPro:   IPR006655
- InterPro:   IPR006311 [H]

Pfam domain/function: PF04879 Molybdop_Fe4S4; PF00384 Molybdopterin; PF01568 Molydop_binding [H]

EC number: 1.8.99.- [C]

Molecular weight: Translated: 89689; Mature: 89558

Theoretical pI: Translated: 8.25; Mature: 8.25

Prosite motif: PS00932 MOLYBDOPTERIN_PROK_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKNEQMVGISRRTLVKSTAIGSLALAAGGFSLPFTLRSSAAAVQQASEKVVWGACSVNC
CCCCCCEECCHHHHHHHHHCCCHHEEECCCCCCCEEHHHHHHHHHHHCCCEEEEEEECCC
GSRCALRLHVKDNEVTWVETDNTGNDEYGNQQVRACLRGRSIRRRINHPDRLNYPMKRVG
CCEEEEEEEEECCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHC
KRGEGKFERISWDEALDTIASSLKKTVEQYGNEAVYIQYSSGIVGGNMTRSSPSASAVKR
CCCCCCEEECCHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCEECCCCCCCCCCHHHHHH
LMNCYGGSLNQYGSYSTAQISCAMPYTYGSNDGNSTTDIENSKLVVMFGNNPAETRMSGG
HHHHHCCCCHHCCCCCCEEEEEEECEEECCCCCCCCCCCCCCEEEEEECCCCHHCEECCC
GITYLLEKAREKSNAKMIVIDPRYTDTAAGREDEWLPIRPGTDAALVAGIAWVLINENLV
CHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCEEECCCCCCHHHHHHEEEEEECCCCC
DQPFLDKYCVGYDEKTLPADAPKNGHYKAYILGEGDDKTAKTPQWASQITGIPVDRIIKL
CCCCHHHHHCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCHHHHHHCCCCHHHHHHH
AREIGTAKPAYICQGWGPQRQANGELTARAIAMLPILTGNVGISGGNSGARESTYTITIE
HHHHCCCCCCEEECCCCCCCCCCCCHHHHHHHHHHEEECCEEECCCCCCCCCCEEEEEEE
RLPVLDNPVKTSISCFSWTDAIDHGPQMTAIRDGVRGKDKLDVPIKFIWNYAGNTLVNQH
ECCCCCCCHHCCEEEEEEHHHHCCCCCCHHHHCCCCCCCCCCCCEEEEEECCCCCEECCC
SDINKTHEILQDESKCEMIVVIENFMTSSAKYADILLPDLMTVEQEDIIPNDYAGNMGYL
CCCHHHHHHHCCCCCCCEEEEEEHHHCCCCCHHHHHHHHHHHCCHHCCCCCCCCCCCEEE
IFLQPVTSEKFERKPIYWILSEVAKRLGPDVYQKFTEGRTQEQWLQHLYAKMLAKDPALP
EEEECCCCCCCCCCCHHHHHHHHHHHHCHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCC
SYDELKKMGIYKRKDPNGHFVAYKAFREDPEANPLKTPSGKIEIYSNRLAEIARTWELEK
CHHHHHHCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCEEEHHHHHHHHHHHCCCCH
DEVISPLPVYASTFEGWDSPERRTFPLQLFGFHYKSRTHSTYGNIDVLKAACRQEVWINP
HHCCCCCCHHHHHCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCC
IDAQKRGIANGDMVRVFNHRGEVRLPAKVTPRILPGVSAMGQGAWHEANMSGDKIDHGGC
CCHHHCCCCCCCEEEEECCCCCEEECCCCCCHHCCCCHHHCCCCCEECCCCCCCCCCCCH
VNTLTTLRPSPLAKGNPQHTNLVEIEKI
HHHHHHCCCCCCCCCCCCCCCEEEEECC
>Mature Secondary Structure 
SKNEQMVGISRRTLVKSTAIGSLALAAGGFSLPFTLRSSAAAVQQASEKVVWGACSVNC
CCCCCEECCHHHHHHHHHCCCHHEEECCCCCCCEEHHHHHHHHHHHCCCEEEEEEECCC
GSRCALRLHVKDNEVTWVETDNTGNDEYGNQQVRACLRGRSIRRRINHPDRLNYPMKRVG
CCEEEEEEEEECCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHC
KRGEGKFERISWDEALDTIASSLKKTVEQYGNEAVYIQYSSGIVGGNMTRSSPSASAVKR
CCCCCCEEECCHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCEECCCCCCCCCCHHHHHH
LMNCYGGSLNQYGSYSTAQISCAMPYTYGSNDGNSTTDIENSKLVVMFGNNPAETRMSGG
HHHHHCCCCHHCCCCCCEEEEEEECEEECCCCCCCCCCCCCCEEEEEECCCCHHCEECCC
GITYLLEKAREKSNAKMIVIDPRYTDTAAGREDEWLPIRPGTDAALVAGIAWVLINENLV
CHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCEEECCCCCCHHHHHHEEEEEECCCCC
DQPFLDKYCVGYDEKTLPADAPKNGHYKAYILGEGDDKTAKTPQWASQITGIPVDRIIKL
CCCCHHHHHCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCHHHHHHCCCCHHHHHHH
AREIGTAKPAYICQGWGPQRQANGELTARAIAMLPILTGNVGISGGNSGARESTYTITIE
HHHHCCCCCCEEECCCCCCCCCCCCHHHHHHHHHHEEECCEEECCCCCCCCCCEEEEEEE
RLPVLDNPVKTSISCFSWTDAIDHGPQMTAIRDGVRGKDKLDVPIKFIWNYAGNTLVNQH
ECCCCCCCHHCCEEEEEEHHHHCCCCCCHHHHCCCCCCCCCCCCEEEEEECCCCCEECCC
SDINKTHEILQDESKCEMIVVIENFMTSSAKYADILLPDLMTVEQEDIIPNDYAGNMGYL
CCCHHHHHHHCCCCCCCEEEEEEHHHCCCCCHHHHHHHHHHHCCHHCCCCCCCCCCCEEE
IFLQPVTSEKFERKPIYWILSEVAKRLGPDVYQKFTEGRTQEQWLQHLYAKMLAKDPALP
EEEECCCCCCCCCCCHHHHHHHHHHHHCHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCC
SYDELKKMGIYKRKDPNGHFVAYKAFREDPEANPLKTPSGKIEIYSNRLAEIARTWELEK
CHHHHHHCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCEEEHHHHHHHHHHHCCCCH
DEVISPLPVYASTFEGWDSPERRTFPLQLFGFHYKSRTHSTYGNIDVLKAACRQEVWINP
HHCCCCCCHHHHHCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCC
IDAQKRGIANGDMVRVFNHRGEVRLPAKVTPRILPGVSAMGQGAWHEANMSGDKIDHGGC
CCHHHCCCCCCCEEEEECCCCCEEECCCCCCHHCCCCHHHCCCCCEECCCCCCCCCCCCH
VNTLTTLRPSPLAKGNPQHTNLVEIEKI
HHHHHHCCCCCCCCCCCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: May Bind 4Fe-4S Cluster. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]