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The map label for this gene is pspE

Identifier: 170682062

GI number: 170682062

Start: 1835846

End: 1836160

Strand: Reverse

Name: pspE

Synonym: EcSMS35_1814

Alternate gene names: 170682062

Gene position: 1836160-1835846 (Counterclockwise)

Preceding gene: 170683517

Following gene: 170679871

Centisome position: 36.23

GC content: 51.43

Gene sequence:

>315_bases
ATGTTTAAAAAAGGCTTACTTGCTCTGGCACTGGTGTTTTCACTGCCCGTTTTCGCCGCTGAACACTGGATCGATGTTCG
TGTTCCTGAGCAGTATCAGCAAGAGCACGTTCAGGGGGCCATCAATATTCCCCTGAAGGAAGTGAAAGAGCGCATTGCCA
CCGCCGTTCCGGATAAAAACGACACCGTGAAAGTGTATTGCAATGCCGGACGCCAGTCAGGGCAGGCAAAAGAGATCCTT
AGCGAGATGGGATATACCCACGTTGAGAACGCCGGCGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAA

Upstream 100 bases:

>100_bases
AACTGGCACAGCGTTATAAAAGGTGAGGGGAATTTCGCAAAAATTGTTAAATCTCAGGCGTATAATGGATGGCAATTTTC
ATCCATAGAAGGACGCTTAC

Downstream 100 bases:

>100_bases
AAGAATCCACCCTAAGCGGCGCTTATGCGCCGTTTACTTTTTCTGTTACACCTTATTTACATCCCCATAGATTATTTGCG
TCAGCTCACAAATACGCTTT

Product: thiosulfate:cyanide sulfurtransferase

Products: NA

Alternate protein names: TST; Phage shock protein E

Number of amino acids: Translated: 104; Mature: 104

Protein sequence:

>104_residues
MFKKGLLALALVFSLPVFAAEHWIDVRVPEQYQQEHVQGAINIPLKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKEIL
SEMGYTHVENAGGLKDIAMPKVKG

Sequences:

>Translated_104_residues
MFKKGLLALALVFSLPVFAAEHWIDVRVPEQYQQEHVQGAINIPLKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKEIL
SEMGYTHVENAGGLKDIAMPKVKG
>Mature_104_residues
MFKKGLLALALVFSLPVFAAEHWIDVRVPEQYQQEHVQGAINIPLKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKEIL
SEMGYTHVENAGGLKDIAMPKVKG

Specific function: The phage shock protein (psp) operon (pspABCDE) may play a significant role in the competition for survival under nutrient- or energy-limited conditions. PspE catalyzes the sulfur-transfer reaction from thiosulfate to cyanide, to form sulfite and thiocyan

COG id: COG0607

COG function: function code P; Rhodanese-related sulfurtransferase

Gene ontology:

Cell location: Periplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 rhodanese domain

Homologues:

Organism=Escherichia coli, GI1787567, Length=104, Percent_Identity=100, Blast_Score=216, Evalue=2e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PSPE_ECOLI (P23857)

Other databases:

- EMBL:   X57560
- EMBL:   U00096
- EMBL:   AP009048
- PIR:   S17125
- RefSeq:   AP_001934.1
- RefSeq:   NP_415824.1
- PDB:   2JTQ
- PDB:   2JTR
- PDB:   2JTS
- PDBsum:   2JTQ
- PDBsum:   2JTR
- PDBsum:   2JTS
- ProteinModelPortal:   P23857
- SMR:   P23857
- DIP:   DIP-10591N
- STRING:   P23857
- EnsemblBacteria:   EBESCT00000002692
- EnsemblBacteria:   EBESCT00000016303
- GeneID:   945652
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW1301
- KEGG:   eco:b1308
- EchoBASE:   EB0773
- EcoGene:   EG10780
- eggNOG:   COG0607
- GeneTree:   EBGT00050000010480
- HOGENOM:   HBG743107
- OMA:   IFIHRRT
- ProtClustDB:   PRK10287
- BioCyc:   EcoCyc:EG10780-MONOMER
- BioCyc:   MetaCyc:EG10780-MONOMER
- Genevestigator:   P23857
- InterPro:   IPR014323
- InterPro:   IPR001763
- Gene3D:   G3DSA:3.40.250.10
- SMART:   SM00450
- TIGRFAMs:   TIGR02981

Pfam domain/function: PF00581 Rhodanese; SSF52821 Rhodanese-like

EC number: =2.8.1.1

Molecular weight: Translated: 11475; Mature: 11475

Theoretical pI: Translated: 8.47; Mature: 8.47

Prosite motif: PS50206 RHODANESE_3

Important sites: ACT_SITE 67-67

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKKGLLALALVFSLPVFAAEHWIDVRVPEQYQQEHVQGAINIPLKEVKERIATAVPDKN
CCCHHHHHHHHHHHHHHHHHHHEEECCCCHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCC
DTVKVYCNAGRQSGQAKEILSEMGYTHVENAGGLKDIAMPKVKG
CEEEEEECCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MFKKGLLALALVFSLPVFAAEHWIDVRVPEQYQQEHVQGAINIPLKEVKERIATAVPDKN
CCCHHHHHHHHHHHHHHHHHHHEEECCCCHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCC
DTVKVYCNAGRQSGQAKEILSEMGYTHVENAGGLKDIAMPKVKG
CEEEEEECCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1712397; 9097039; 9278503; 9868784