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The map label for this gene is ycjM [H]

Identifier: 170679871

GI number: 170679871

Start: 1833953

End: 1835632

Strand: Reverse

Name: ycjM [H]

Synonym: EcSMS35_1813

Alternate gene names: 170679871

Gene position: 1835632-1833953 (Counterclockwise)

Preceding gene: 170682062

Following gene: 170683434

Centisome position: 36.22

GC content: 44.23

Gene sequence:

>1680_bases
ATGAAACAGAAAATTACGGATTACCTGGACGAAATCTACGGTGGAACATTTACCGCAACTCATTTACAGAAACTTGTAAC
GCGTCTTGAGAGTGCGAAACGATTAATTACACTGCGACGTAAAAAACACTGGGATGAAAGTGATGTCGTGTTAATCACCT
ATGCCGATCAATTTCACAGTAATGATTTAAAACCACTACCCACATTTAATCAGTTTTACCCTCAATGGCTGCAAAGCATT
TTTTCACATGTTCATTTATTACCTTTTTATCCATGGTCATCTGATGATGGCTTTTCGGTAATTGATTATCAACAGGTCGC
TAGTGAAGCGGGGGAGTGGCAGGATATTCAGCAACTCGGTGAATGCAGTCATTTAATGTTTGATTTTGTCTGCAACCATA
TGTCGGCAAAAAGTGAATGGTTTAAAAACTATTTACAACAGCAGCCAGGTTTTGAAGATTTTTTTATTGCCGTTGACCCG
CAAACCGATCTCAGCGTCGTCACTCGCCCGCGTGCGTTACCGTTATTAACGCCATTCCAGATGCGCGATCATTCAACGCG
CCATTTATGGACCACCTTTAGTGACGATCAAATTGACCTGAATTACCGTAGCCCTGAAGTGTTACTGGCGATGGTGGATG
TATTGCTGTGCTACCTGGAAAAGGGCGCAGAATATGTCCGTCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACA
AGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATGACGTAGCACCAGGTACAGT
GATCATTACCGAGACCAATGTTCCGCACAGAGACAACATTGCTTACTTTGGCAACGGTGATGACGAAGCGCATATGGTGT
ACCAGTTCTCGCTGCCGCCGCTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTTGTGCGTGGGCGCAAAAC
CTGACACTACCTTCCAGCAACACCACCTGGTTTAACTTCCTCGCCTCTCACGATGGCATCGGGCTTAACCCACTGCGTGG
CTTGCTACCCGAAAGCGAAATATTAGCGCTGGTCGAGGCATTACAGCAGGAAGGGGCATTAGTTAACTGGAAAAATAATC
CCGACGGTACGCGTAGCCCGTATGAAATGAATGTCACTTATATGGATGCGTTAAACCGCCGCGAGAGTAGCGATGAAGAA
CGTTGCGCCAGGTTTATCCTTGCCCATGCGATTTTGTTAAGTTTCCCCGGTGTGCCAGCGATATATATTCAAAGTATTCT
GGGCTCGCGTAATGATTACGCAGGTGTCGAAAAACTGGGATATAACCGTGCGATTAACCGTAAAAAATATTACAGTAAAG
AGATCACGACCGAACTGAACAATAAAACGACGTTAAGGCACGCGGTATATCATGAATTGTCGCGACTAATTAAAATTCGT
CGAAGTCATAACGAGTTTCATCCAGATAATGATTTTACCATCGACACGGTTAATTCATCCGTAATGTGTATTCAAAGAAG
CAACGCGGATGGTAATTGTCTGACAGGATTGTTTAATGTCAGTGAAAATATTCAGCATATAAATATTACTGACCTGCACG
GTCGGGATCTGATTAGTGAAGTTGATATAGTGGGTAATGAAATAACGCTGCGCCCCTGGCAGGTTATGTGGATTAAATAA

Upstream 100 bases:

>100_bases
AATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGTTTATTTTCTGCGGAGTAGTCTCTCGTTTCATGGGAC
CGCTACCACGGAAAGGCAAC

Downstream 100 bases:

>100_bases
AAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCCTGCGCCCTGATTTCAGGTTGTAAAG
AAGAAAATAAAACGAATGTC

Product: alpha amylase family protein

Products: NA

Alternate protein names: Sucrose glucosyltransferase [H]

Number of amino acids: Translated: 559; Mature: 559

Protein sequence:

>559_residues
MKQKITDYLDEIYGGTFTATHLQKLVTRLESAKRLITLRRKKHWDESDVVLITYADQFHSNDLKPLPTFNQFYPQWLQSI
FSHVHLLPFYPWSSDDGFSVIDYQQVASEAGEWQDIQQLGECSHLMFDFVCNHMSAKSEWFKNYLQQQPGFEDFFIAVDP
QTDLSVVTRPRALPLLTPFQMRDHSTRHLWTTFSDDQIDLNYRSPEVLLAMVDVLLCYLEKGAEYVRLDAVGFMWKEPGT
SCIHLEKTHLIIKLLRSIIDDVAPGTVIITETNVPHRDNIAYFGNGDDEAHMVYQFSLPPLVLHAVQKQNVEALCAWAQN
LTLPSSNTTWFNFLASHDGIGLNPLRGLLPESEILALVEALQQEGALVNWKNNPDGTRSPYEMNVTYMDALNRRESSDEE
RCARFILAHAILLSFPGVPAIYIQSILGSRNDYAGVEKLGYNRAINRKKYYSKEITTELNNKTTLRHAVYHELSRLIKIR
RSHNEFHPDNDFTIDTVNSSVMCIQRSNADGNCLTGLFNVSENIQHINITDLHGRDLISEVDIVGNEITLRPWQVMWIK

Sequences:

>Translated_559_residues
MKQKITDYLDEIYGGTFTATHLQKLVTRLESAKRLITLRRKKHWDESDVVLITYADQFHSNDLKPLPTFNQFYPQWLQSI
FSHVHLLPFYPWSSDDGFSVIDYQQVASEAGEWQDIQQLGECSHLMFDFVCNHMSAKSEWFKNYLQQQPGFEDFFIAVDP
QTDLSVVTRPRALPLLTPFQMRDHSTRHLWTTFSDDQIDLNYRSPEVLLAMVDVLLCYLEKGAEYVRLDAVGFMWKEPGT
SCIHLEKTHLIIKLLRSIIDDVAPGTVIITETNVPHRDNIAYFGNGDDEAHMVYQFSLPPLVLHAVQKQNVEALCAWAQN
LTLPSSNTTWFNFLASHDGIGLNPLRGLLPESEILALVEALQQEGALVNWKNNPDGTRSPYEMNVTYMDALNRRESSDEE
RCARFILAHAILLSFPGVPAIYIQSILGSRNDYAGVEKLGYNRAINRKKYYSKEITTELNNKTTLRHAVYHELSRLIKIR
RSHNEFHPDNDFTIDTVNSSVMCIQRSNADGNCLTGLFNVSENIQHINITDLHGRDLISEVDIVGNEITLRPWQVMWIK
>Mature_559_residues
MKQKITDYLDEIYGGTFTATHLQKLVTRLESAKRLITLRRKKHWDESDVVLITYADQFHSNDLKPLPTFNQFYPQWLQSI
FSHVHLLPFYPWSSDDGFSVIDYQQVASEAGEWQDIQQLGECSHLMFDFVCNHMSAKSEWFKNYLQQQPGFEDFFIAVDP
QTDLSVVTRPRALPLLTPFQMRDHSTRHLWTTFSDDQIDLNYRSPEVLLAMVDVLLCYLEKGAEYVRLDAVGFMWKEPGT
SCIHLEKTHLIIKLLRSIIDDVAPGTVIITETNVPHRDNIAYFGNGDDEAHMVYQFSLPPLVLHAVQKQNVEALCAWAQN
LTLPSSNTTWFNFLASHDGIGLNPLRGLLPESEILALVEALQQEGALVNWKNNPDGTRSPYEMNVTYMDALNRRESSDEE
RCARFILAHAILLSFPGVPAIYIQSILGSRNDYAGVEKLGYNRAINRKKYYSKEITTELNNKTTLRHAVYHELSRLIKIR
RSHNEFHPDNDFTIDTVNSSVMCIQRSNADGNCLTGLFNVSENIQHINITDLHGRDLISEVDIVGNEITLRPWQVMWIK

Specific function: Unknown

COG id: COG0366

COG function: function code G; Glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sucrose phosphorylase family [H]

Homologues:

Organism=Escherichia coli, GI87081873, Length=559, Percent_Identity=95.169946332737, Blast_Score=1116, Evalue=0.0,
Organism=Drosophila melanogaster, GI24583749, Length=172, Percent_Identity=31.9767441860465, Blast_Score=86, Evalue=6e-17,
Organism=Drosophila melanogaster, GI24583747, Length=172, Percent_Identity=31.9767441860465, Blast_Score=86, Evalue=6e-17,
Organism=Drosophila melanogaster, GI24583745, Length=236, Percent_Identity=26.6949152542373, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI45549022, Length=177, Percent_Identity=26.5536723163842, Blast_Score=67, Evalue=3e-11,
Organism=Drosophila melanogaster, GI221330053, Length=166, Percent_Identity=28.3132530120482, Blast_Score=67, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006047
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR016377 [H]

Pfam domain/function: PF00128 Alpha-amylase [H]

EC number: =2.4.1.7 [H]

Molecular weight: Translated: 64252; Mature: 64252

Theoretical pI: Translated: 5.84; Mature: 5.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQKITDYLDEIYGGTFTATHLQKLVTRLESAKRLITLRRKKHWDESDVVLITYADQFHS
CCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCC
NDLKPLPTFNQFYPQWLQSIFSHVHLLPFYPWSSDDGFSVIDYQQVASEAGEWQDIQQLG
CCCCCCCCHHHHHHHHHHHHHHHHEEEEECCCCCCCCCEEEEHHHHHHCCCCHHHHHHHH
ECSHLMFDFVCNHMSAKSEWFKNYLQQQPGFEDFFIAVDPQTDLSVVTRPRALPLLTPFQ
HHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHCCCCCCCCCCCHH
MRDHSTRHLWTTFSDDQIDLNYRSPEVLLAMVDVLLCYLEKGAEYVRLDAVGFMWKEPGT
HCCCCCCEEEEECCCCEEEECCCCHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEECCCCC
SCIHLEKTHLIIKLLRSIIDDVAPGTVIITETNVPHRDNIAYFGNGDDEAHMVYQFSLPP
CEEEEHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEEECCCCCCEEEEEEECCCH
LVLHAVQKQNVEALCAWAQNLTLPSSNTTWFNFLASHDGIGLNPLRGLLPESEILALVEA
HHHHHHHHCCHHHHHHHHHHCCCCCCCCHHEEEECCCCCCCCCHHHHCCCHHHHHHHHHH
LQQEGALVNWKNNPDGTRSPYEMNVTYMDALNRRESSDEERCARFILAHAILLSFPGVPA
HHHCCCEEEECCCCCCCCCCEEEEEHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCHH
IYIQSILGSRNDYAGVEKLGYNRAINRKKYYSKEITTELNNKTTLRHAVYHELSRLIKIR
HHHHHHHCCCCCCCHHHHHCCHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
RSHNEFHPDNDFTIDTVNSSVMCIQRSNADGNCLTGLFNVSENIQHINITDLHGRDLISE
HHCCCCCCCCCEEEEECCCEEEEEEECCCCCCCEEEEHHHCCCCEEEEEECCCCHHHHHH
VDIVGNEITLRPWQVMWIK
HHHCCCEEEECCEEEEEEC
>Mature Secondary Structure
MKQKITDYLDEIYGGTFTATHLQKLVTRLESAKRLITLRRKKHWDESDVVLITYADQFHS
CCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCC
NDLKPLPTFNQFYPQWLQSIFSHVHLLPFYPWSSDDGFSVIDYQQVASEAGEWQDIQQLG
CCCCCCCCHHHHHHHHHHHHHHHHEEEEECCCCCCCCCEEEEHHHHHHCCCCHHHHHHHH
ECSHLMFDFVCNHMSAKSEWFKNYLQQQPGFEDFFIAVDPQTDLSVVTRPRALPLLTPFQ
HHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHCCCCCCCCCCCHH
MRDHSTRHLWTTFSDDQIDLNYRSPEVLLAMVDVLLCYLEKGAEYVRLDAVGFMWKEPGT
HCCCCCCEEEEECCCCEEEECCCCHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEECCCCC
SCIHLEKTHLIIKLLRSIIDDVAPGTVIITETNVPHRDNIAYFGNGDDEAHMVYQFSLPP
CEEEEHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEEECCCCCCEEEEEEECCCH
LVLHAVQKQNVEALCAWAQNLTLPSSNTTWFNFLASHDGIGLNPLRGLLPESEILALVEA
HHHHHHHHCCHHHHHHHHHHCCCCCCCCHHEEEECCCCCCCCCHHHHCCCHHHHHHHHHH
LQQEGALVNWKNNPDGTRSPYEMNVTYMDALNRRESSDEERCARFILAHAILLSFPGVPA
HHHCCCEEEECCCCCCCCCCEEEEEHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCHH
IYIQSILGSRNDYAGVEKLGYNRAINRKKYYSKEITTELNNKTTLRHAVYHELSRLIKIR
HHHHHHHCCCCCCCHHHHHCCHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
RSHNEFHPDNDFTIDTVNSSVMCIQRSNADGNCLTGLFNVSENIQHINITDLHGRDLISE
HHCCCCCCCCCEEEEECCCEEEEEEECCCCCCCEEEEHHHCCCCEEEEEECCCCHHHHHH
VDIVGNEITLRPWQVMWIK
HHHCCCEEEECCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]