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The map label for this gene is sdaA

Identifier: 170681757

GI number: 170681757

Start: 1361797

End: 1363161

Strand: Reverse

Name: sdaA

Synonym: EcSMS35_1374

Alternate gene names: 170681757

Gene position: 1363161-1361797 (Counterclockwise)

Preceding gene: 170680320

Following gene: 170681526

Centisome position: 26.9

GC content: 54.87

Gene sequence:

>1365_bases
GTGATTAGTCTATTCGACATGTTTAAGGTGGGGATTGGTCCCTCATCTTCCCATACCGTAGGGCCTATGAAGGCGGGTAA
ACAGTTCGTCGATGACCTGGTCGAAAAAGGCTTACTGGATAGCGTTACTCGCGTTGCCGTGGACGTTTATGGTTCACTGT
CGCTGACGGGTAAAGGCCACCACACCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATC
GACAGTATTCCCGGTTTTATTCGCGACGTAGAAGAGCGCGAACGTCTGCTGCTGGCACAGGGACGGCATGAAGTGGATTT
CCCGCGCGACAACGGGATGCGTTTTCATAACGGTAACCTGCCGCTGCATGAAAACGGTATGCAAATCCACGCCTATAACG
GCGATGAAGTCGTCTACAGCAAAACTTATTATTCCATCGGCGGCGGTTTTATCGTCGATGAAGAACACTTTGGTCAGGAT
GCCGCCAACGAAGTGAGCGTGCCGTATCCGTTCAAATCTGCCACCGAACTGCTCGCGTACTGCAATGAAACCGGCTATTC
GCTGTCTGGTCTCGCTATGCAGAATGAACTGGCGCTGCACAGCAAGAAAGAGATCGACGAGTATTTCGCGCATGTCTGGC
AAACCATGCAGGCATGTATCGATCGCGGGATGAACACCGAAGGCGTACTGCCAGGCCCGCTGCGCGTGCCGCGTCGTGCG
TCTGCCCTGCGCCGGATGCTGGTTTCCAGCGATAAACTGTCTAACGATCCGATGAATGTCATTGACTGGGTAAACATGTT
TGCGCTGGCAGTTAACGAAGAGAACGCCGCCGGTGGTCGCGTGGTAACTGCGCCAACCAACGGTGCTTGTGGCATTGTTC
CGGCAGTGCTGGCTTACTATGACCACTTTATTGAGTCCGTCAGCCCGGACATCTATACCCGCTACTTTATGGCAGCGGGT
GCGATTGGTGCACTGTATAAAATGAACGCCTCTATTTCCGGTGCGGAAGTCGGTTGCCAGGGCGAAGTGGGTGTTGCCTG
TTCAATGGCTGCTGCGGGCCTTGCCGAGCTGTTGGGCGGTAGCCCGGAACAGGTTTGTGTGGCGGCGGAAATTGGCATGG
AGCACAACCTCGGTCTGACCTGCGACCCGGTTGCAGGGCAGGTTCAGGTGCCGTGCATTGAGCGTAATGCTATTGCCTCT
GTGAAGGCGATTAACGCCGCACGGATGGCTCTGCGCCGCACCAGCGCACCGCGCGTCTCGCTGGATAAGGTCATCGAAAC
GATGTACGAAACCGGTAAGGACATGAACGCCAAATACCGCGAAACCTCACGCGGTGGTCTGGCAATCAAAGTCCAGTGTG
ACTAA

Upstream 100 bases:

>100_bases
TTCATGTGAATAGTTAAGCCAGTCGCCGCGTTCCCTCTTACACTATGCGCTGTTATTAGTTCGTTACTGGAAGTCCAGTC
ACCTTGTCAGGAGTATTATC

Downstream 100 bases:

>100_bases
TACTTCTTACTCGCCCATCTGCAACGGATGGGCGAATTTATCCCCGCTTTCTCGTCTGCTGTAATATTCCCCACTACACT
TCCACTGTTGCGTCAGGCGT

Product: L-serine ammonia-lyase 1

Products: NA

Alternate protein names: SDH 1; L-serine deaminase 1; L-SD1

Number of amino acids: Translated: 454; Mature: 454

Protein sequence:

>454_residues
MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTRVAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDI
DSIPGFIRDVEERERLLLAQGRHEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQACIDRGMNTEGVLPGPLRVPRRA
SALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENAAGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAG
AIGALYKMNASISGAEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPCIERNAIAS
VKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIKVQCD

Sequences:

>Translated_454_residues
MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTRVAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDI
DSIPGFIRDVEERERLLLAQGRHEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQACIDRGMNTEGVLPGPLRVPRRA
SALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENAAGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAG
AIGALYKMNASISGAEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPCIERNAIAS
VKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIKVQCD
>Mature_454_residues
MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTRVAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDI
DSIPGFIRDVEERERLLLAQGRHEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQACIDRGMNTEGVLPGPLRVPRRA
SALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENAAGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAG
AIGALYKMNASISGAEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPCIERNAIAS
VKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIKVQCD

Specific function: Deaminates also threonine, particularly when it is present in high concentration

COG id: COG1760

COG function: function code E; L-serine deaminase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the iron-sulfur dependent L-serine dehydratase family

Homologues:

Organism=Escherichia coli, GI1788116, Length=454, Percent_Identity=100, Blast_Score=941, Evalue=0.0,
Organism=Escherichia coli, GI1789161, Length=455, Percent_Identity=77.3626373626374, Blast_Score=736, Evalue=0.0,
Organism=Escherichia coli, GI48994925, Length=453, Percent_Identity=73.9514348785872, Blast_Score=681, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SDHL_ECOLI (P16095)

Other databases:

- EMBL:   M28695
- EMBL:   U00096
- EMBL:   AP009048
- PIR:   F64942
- RefSeq:   AP_002433.1
- RefSeq:   NP_416328.1
- ProteinModelPortal:   P16095
- SMR:   P16095
- STRING:   P16095
- EnsemblBacteria:   EBESCT00000001075
- EnsemblBacteria:   EBESCT00000016151
- GeneID:   946331
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW1803
- KEGG:   eco:b1814
- EchoBASE:   EB0923
- EcoGene:   EG10930
- eggNOG:   COG1760
- GeneTree:   EBGT00050000009684
- HOGENOM:   HBG304565
- OMA:   INDFLLT
- ProtClustDB:   PRK15023
- BioCyc:   EcoCyc:LSERINEDEAM1-MONOMER
- BioCyc:   MetaCyc:LSERINEDEAM1-MONOMER
- Genevestigator:   P16095
- GO:   GO:0006094
- InterPro:   IPR004644
- InterPro:   IPR005130
- InterPro:   IPR005131
- TIGRFAMs:   TIGR00720

Pfam domain/function: PF03313 SDH_alpha; PF03315 SDH_beta

EC number: =4.3.1.17

Molecular weight: Translated: 48907; Mature: 48907

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTRVAVDVYGSLSLTGKGH
CCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCC
HTDIAIIMGLAGNEPATVDIDSIPGFIRDVEERERLLLAQGRHEVDFPRDNGMRFHNGNL
CCEEEEEEEECCCCCCEEEHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEECCCC
PLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQDAANEVSVPYPFKSATELLAY
CEECCCCEEEEECCCEEEEEEEEEECCCCEEECHHHCCCCCCCCCCCCCCCHHHHHHHHH
CNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQACIDRGMNTEGVLPGPLRVPRRA
HCCCCCCEECEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHH
SALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENAAGGRVVTAPTNGACGIVPAVLAYY
HHHHHHHHHCCCCCCCCHHHHHHHHHHEEEECCCCCCCCEEEECCCCCCCHHHHHHHHHH
DHFIESVSPDIYTRYFMAAGAIGALYKMNASISGAEVGCQGEVGVACSMAAAGLAELLGG
HHHHHHCCCHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCC
SPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPCIERNAIASVKAINAARMALRRTSAPRVS
CCHHEEEEEECCCCCCCCEEECCCCCCEECCEECCCHHHHHHHHHHHHHHHHHCCCCCCC
LDKVIETMYETGKDMNAKYRETSRGGLAIKVQCD
HHHHHHHHHHCCCCCCCHHHCCCCCCEEEEEEEC
>Mature Secondary Structure
MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTRVAVDVYGSLSLTGKGH
CCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCC
HTDIAIIMGLAGNEPATVDIDSIPGFIRDVEERERLLLAQGRHEVDFPRDNGMRFHNGNL
CCEEEEEEEECCCCCCEEEHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEECCCC
PLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQDAANEVSVPYPFKSATELLAY
CEECCCCEEEEECCCEEEEEEEEEECCCCEEECHHHCCCCCCCCCCCCCCCHHHHHHHHH
CNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQACIDRGMNTEGVLPGPLRVPRRA
HCCCCCCEECEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHH
SALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENAAGGRVVTAPTNGACGIVPAVLAYY
HHHHHHHHHCCCCCCCCHHHHHHHHHHEEEECCCCCCCCEEEECCCCCCCHHHHHHHHHH
DHFIESVSPDIYTRYFMAAGAIGALYKMNASISGAEVGCQGEVGVACSMAAAGLAELLGG
HHHHHHCCCHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCC
SPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPCIERNAIASVKAINAARMALRRTSAPRVS
CCHHEEEEEECCCCCCCCEEECCCCCCEECCEECCCHHHHHHHHHHHHHHHHHCCCCCCC
LDKVIETMYETGKDMNAKYRETSRGGLAIKVQCD
HHHHHHHHHHCCCCCCCHHHCCCCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2504697; 8436113; 9097040; 9278503