Click here to switch to the map view.

The map label for this gene is ycjZ [H]

Identifier: 170681201

GI number: 170681201

Start: 1812365

End: 1813264

Strand: Reverse

Name: ycjZ [H]

Synonym: EcSMS35_1793

Alternate gene names: 170681201

Gene position: 1813264-1812365 (Counterclockwise)

Preceding gene: 170681912

Following gene: 170680564

Centisome position: 35.78

GC content: 46.78

Gene sequence:

>900_bases
ATGAAGCGTGAAGAAATCGCTGATCTGATGGCGTTTGTCGTTGTTGCAGAGGAGCGTAGCTTCACTCGCGCAGCAGCCCG
CCTGAGCATGGCGCAGTCAGCTTTAAGCCAGATAGTGCGCCGTATAGAAGAACGATTGGGATTGCGGCTTCTGACGCGAA
CCACGCGCAGCGTTGTACCCACTGAAGCGGGCGAGCATCTTTTGTCTGTTCTTGGCCCTATGTTGCATGACATAGATTCT
GCCCTGACATCCCTGAGCGATCTGCAGAACCGCCCATCCGGGACAATACGTATTACTACTGTAGAACATGCAGCAAAAAC
GATATTGTTACCAGCTATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTTACCATTGATTATGGTTTGA
CCGATGTCGTTTCTGAACGTTTTGATGCAGGCGTCCGTCTGGGTGGGGAGATGGATAAAGATATGATCGCCATTCGAATC
GGGCCAGACATACCAATGGCTATTGTTGGCTCACCGGATTATTTTTCTCGTCGAAGTGCTCCAACGTCAGTGTCACAATT
AATAGATCATCAGGCAATTAATTTGTATCTTCCCACATCGGGTACAGCAAATCGCTGGAGATTAATACGCGGTGGACGTG
AAGTTCGTGTTCGCATGGAAGGTCAGCTTTTACTGAATACGATAGACCTGATCATTGATGCTGCAATTGATGGGCATGGA
TTGGCGTATCTACCTTATGATCAGGTTGAGCGGGCTATTAAAGAAAAAAAACTGATACGTGTTTTGGATAAATTCACACC
AGATTTACCCGGTTATCACCTGTACTATCCACACCGTCGACATGCTGGCTCGGCATTCTCATTATTTATAGATAGGCTGA
AGTATAAAGGTGCTGTTTAG

Upstream 100 bases:

>100_bases
TGGTGATATCACTATAGATATTGATCATTAAGTTGATTAGACCCAAAATCATGATTAGACCTATCATTTAAATGATTAAT
AGATTGTATGGAGATACTGA

Downstream 100 bases:

>100_bases
CACTACTTGCTGATACATTAATTTAATTCTTCTCTTAACGTATTCTCAGTTCCTTTCAACGTTTTGGTTATTTTTTATTC
TTCGCACAATGGCGACAGAT

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 299; Mature: 299

Protein sequence:

>299_residues
MKREEIADLMAFVVVAEERSFTRAAARLSMAQSALSQIVRRIEERLGLRLLTRTTRSVVPTEAGEHLLSVLGPMLHDIDS
ALTSLSDLQNRPSGTIRITTVEHAAKTILLPAMRTFLKSHPEIDIQLTIDYGLTDVVSERFDAGVRLGGEMDKDMIAIRI
GPDIPMAIVGSPDYFSRRSAPTSVSQLIDHQAINLYLPTSGTANRWRLIRGGREVRVRMEGQLLLNTIDLIIDAAIDGHG
LAYLPYDQVERAIKEKKLIRVLDKFTPDLPGYHLYYPHRRHAGSAFSLFIDRLKYKGAV

Sequences:

>Translated_299_residues
MKREEIADLMAFVVVAEERSFTRAAARLSMAQSALSQIVRRIEERLGLRLLTRTTRSVVPTEAGEHLLSVLGPMLHDIDS
ALTSLSDLQNRPSGTIRITTVEHAAKTILLPAMRTFLKSHPEIDIQLTIDYGLTDVVSERFDAGVRLGGEMDKDMIAIRI
GPDIPMAIVGSPDYFSRRSAPTSVSQLIDHQAINLYLPTSGTANRWRLIRGGREVRVRMEGQLLLNTIDLIIDAAIDGHG
LAYLPYDQVERAIKEKKLIRVLDKFTPDLPGYHLYYPHRRHAGSAFSLFIDRLKYKGAV
>Mature_299_residues
MKREEIADLMAFVVVAEERSFTRAAARLSMAQSALSQIVRRIEERLGLRLLTRTTRSVVPTEAGEHLLSVLGPMLHDIDS
ALTSLSDLQNRPSGTIRITTVEHAAKTILLPAMRTFLKSHPEIDIQLTIDYGLTDVVSERFDAGVRLGGEMDKDMIAIRI
GPDIPMAIVGSPDYFSRRSAPTSVSQLIDHQAINLYLPTSGTANRWRLIRGGREVRVRMEGQLLLNTIDLIIDAAIDGHG
LAYLPYDQVERAIKEKKLIRVLDKFTPDLPGYHLYYPHRRHAGSAFSLFIDRLKYKGAV

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787589, Length=299, Percent_Identity=98.9966555183946, Blast_Score=598, Evalue=1e-172,
Organism=Escherichia coli, GI1787128, Length=292, Percent_Identity=33.5616438356164, Blast_Score=202, Evalue=3e-53,
Organism=Escherichia coli, GI1786401, Length=288, Percent_Identity=28.8194444444444, Blast_Score=118, Evalue=5e-28,
Organism=Escherichia coli, GI1789639, Length=274, Percent_Identity=28.1021897810219, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI145693193, Length=303, Percent_Identity=29.042904290429, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI87081978, Length=266, Percent_Identity=24.812030075188, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI1789440, Length=272, Percent_Identity=23.5294117647059, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1786448, Length=291, Percent_Identity=25.7731958762887, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI145693105, Length=195, Percent_Identity=26.1538461538462, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI2367136, Length=123, Percent_Identity=35.7723577235772, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1788748, Length=122, Percent_Identity=33.6065573770492, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1787879, Length=94, Percent_Identity=42.5531914893617, Blast_Score=68, Evalue=9e-13,
Organism=Escherichia coli, GI157672245, Length=116, Percent_Identity=34.4827586206897, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1788706, Length=248, Percent_Identity=27.4193548387097, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1788297, Length=250, Percent_Identity=26.8, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33466; Mature: 33466

Theoretical pI: Translated: 9.46; Mature: 9.46

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKREEIADLMAFVVVAEERSFTRAAARLSMAQSALSQIVRRIEERLGLRLLTRTTRSVVP
CCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCC
TEAGEHLLSVLGPMLHDIDSALTSLSDLQNRPSGTIRITTVEHAAKTILLPAMRTFLKSH
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHHHHHCC
PEIDIQLTIDYGLTDVVSERFDAGVRLGGEMDKDMIAIRIGPDIPMAIVGSPDYFSRRSA
CCEEEEEEECCCHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCEEEECCCCHHHCCCC
PTSVSQLIDHQAINLYLPTSGTANRWRLIRGGREVRVRMEGQLLLNTIDLIIDAAIDGHG
CHHHHHHHCCCEEEEEECCCCCCCCEEEEECCCEEEEEECCHHHHHHHHHHHHHCCCCCC
LAYLPYDQVERAIKEKKLIRVLDKFTPDLPGYHLYYPHRRHAGSAFSLFIDRLKYKGAV
EEECCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKREEIADLMAFVVVAEERSFTRAAARLSMAQSALSQIVRRIEERLGLRLLTRTTRSVVP
CCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCC
TEAGEHLLSVLGPMLHDIDSALTSLSDLQNRPSGTIRITTVEHAAKTILLPAMRTFLKSH
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHHHHHCC
PEIDIQLTIDYGLTDVVSERFDAGVRLGGEMDKDMIAIRIGPDIPMAIVGSPDYFSRRSA
CCEEEEEEECCCHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCEEEECCCCHHHCCCC
PTSVSQLIDHQAINLYLPTSGTANRWRLIRGGREVRVRMEGQLLLNTIDLIIDAAIDGHG
CHHHHHHHCCCEEEEEECCCCCCCCEEEEECCCEEEEEECCHHHHHHHHHHHHHCCCCCC
LAYLPYDQVERAIKEKKLIRVLDKFTPDLPGYHLYYPHRRHAGSAFSLFIDRLKYKGAV
EEECCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]