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The map label for this gene is gadX [H]

Identifier: 170681182

GI number: 170681182

Start: 3885632

End: 3886456

Strand: Reverse

Name: gadX [H]

Synonym: EcSMS35_3819

Alternate gene names: 170681182

Gene position: 3886456-3885632 (Counterclockwise)

Preceding gene: 170679884

Following gene: 170680629

Centisome position: 76.68

GC content: 41.21

Gene sequence:

>825_bases
ATGCAACCACTACATGGTAATTGTCTAATTGCGTACGCAAGACATAAATATATTCTCACCATGGTTAATGGTGAATATCG
CTATTTTAATGGCGGTGATCTGGTTTTTGCGGATGCAAGCCAAATTCAAGTGGATAAGTGTGTTGAGAATTTCGTATTAG
TTTCAAGAGACACGCTTTCATTATTTCTCCCGATGCTCAAGGAGGAGGCATTAAAACTTCATGCACATAAAAAAGTTCCT
TCATTACTCGTTCATCACTGTACCAGAGATATTCCTGTTTTTCAGGAAGTTGCGCAACTATCGCAGAATAAGAATCTTCG
CTATGCAGAAATGCTACGTAAAAGGGCATTAATCTTTGCGTTGTTATCTGTTTTTCTTGAGGATACGCAGTTTATACCGT
TACTTCTGAACGTGTTGCAACCGAATATGCGAACACGAGTTTGTACGGTCATCAATAATAATATCGCCCATGAGTGGACA
TTAGCCCGAATTGCTAGCGAATTGTTGATGAGTCCAAGCCTGTTAAAGAAAAAATTGCGCGAAGAAGGTACGTCATATTC
ACAGTTGCTTACTGAGTGTAGGATGCAGCGTGCTTTACAACTTATTGTTATATATGGGGTTTCAATTAAGCGAGTTGCAG
TATCCTGTGGATATCACAGCGTGTCGTATTTCATTTACGTTTTTCGAAATTATTATGGGATGACGCCCACAGAGTATCAG
GAGCGATCGGCGCAGGAATTACCGAACTGTGGCCCGGCGGCAAGTATAGCTGCGCAAGGGAATTTTTACGGCACTGACCG
TTCTGCGGAAGGAATAAGATTATAG

Upstream 100 bases:

>100_bases
TTCCCGCGTCGTTTAAATTTATTTATCAATCAATTTGACTTAAGAGGGCGGCGTGCTACATTAATAAACAGTAATATGTT
TATGTAATATTAAGTCAACT

Downstream 100 bases:

>100_bases
AGTTTTACTCAGACATAAAAAAAACCCGGCATAGGGGACCGGGAAGAGGATAGTCTGCCGTCTCCAGACTAATAAACCGT
TATAACACTCCCTGTTGGCA

Product: DNA-binding transcriptional regulator GadX

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 274; Mature: 274

Protein sequence:

>274_residues
MQPLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIQVDKCVENFVLVSRDTLSLFLPMLKEEALKLHAHKKVP
SLLVHHCTRDIPVFQEVAQLSQNKNLRYAEMLRKRALIFALLSVFLEDTQFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
LARIASELLMSPSLLKKKLREEGTSYSQLLTECRMQRALQLIVIYGVSIKRVAVSCGYHSVSYFIYVFRNYYGMTPTEYQ
ERSAQELPNCGPAASIAAQGNFYGTDRSAEGIRL

Sequences:

>Translated_274_residues
MQPLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIQVDKCVENFVLVSRDTLSLFLPMLKEEALKLHAHKKVP
SLLVHHCTRDIPVFQEVAQLSQNKNLRYAEMLRKRALIFALLSVFLEDTQFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
LARIASELLMSPSLLKKKLREEGTSYSQLLTECRMQRALQLIVIYGVSIKRVAVSCGYHSVSYFIYVFRNYYGMTPTEYQ
ERSAQELPNCGPAASIAAQGNFYGTDRSAEGIRL
>Mature_274_residues
MQPLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIQVDKCVENFVLVSRDTLSLFLPMLKEEALKLHAHKKVP
SLLVHHCTRDIPVFQEVAQLSQNKNLRYAEMLRKRALIFALLSVFLEDTQFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
LARIASELLMSPSLLKKKLREEGTSYSQLLTECRMQRALQLIVIYGVSIKRVAVSCGYHSVSYFIYVFRNYYGMTPTEYQ
ERSAQELPNCGPAASIAAQGNFYGTDRSAEGIRL

Specific function: Positively regulates the expression of about fifteen genes involved in acid resistance such as gadA, gadB and gadC. Depending on the conditions (growth phase and medium), can repress gadW. Negatively regulates perA expression in acidic conditions and posi

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789933, Length=274, Percent_Identity=93.7956204379562, Blast_Score=531, Evalue=1e-152,
Organism=Escherichia coli, GI1790557, Length=223, Percent_Identity=35.8744394618834, Blast_Score=132, Evalue=2e-32,
Organism=Escherichia coli, GI1786778, Length=194, Percent_Identity=39.1752577319588, Blast_Score=128, Evalue=3e-31,
Organism=Escherichia coli, GI1787776, Length=121, Percent_Identity=45.4545454545455, Blast_Score=99, Evalue=2e-22,
Organism=Escherichia coli, GI1786776, Length=132, Percent_Identity=40.1515151515151, Blast_Score=95, Evalue=4e-21,
Organism=Escherichia coli, GI1789932, Length=127, Percent_Identity=38.5826771653543, Blast_Score=92, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR018062
- InterPro:   IPR020449
- InterPro:   IPR018060 [H]

Pfam domain/function: PF00165 HTH_AraC [H]

EC number: NA

Molecular weight: Translated: 31272; Mature: 31272

Theoretical pI: Translated: 8.96; Mature: 8.96

Prosite motif: PS00041 HTH_ARAC_FAMILY_1 ; PS01124 HTH_ARAC_FAMILY_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQPLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIQVDKCVENFVLVSRDTLS
CCCCCCCEEEEEECCEEEEEEECCCEEEECCCCEEEECCCHHHHHHHHHHHHHHCCHHHH
LFLPMLKEEALKLHAHKKVPSLLVHHCTRDIPVFQEVAQLSQNKNLRYAEMLRKRALIFA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
LLSVFLEDTQFIPLLLNVLQPNMRTRVCTVINNNIAHEWTLARIASELLMSPSLLKKKLR
HHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHHHHHHHH
EEGTSYSQLLTECRMQRALQLIVIYGVSIKRVAVSCGYHSVSYFIYVFRNYYGMTPTEYQ
HCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCHHH
ERSAQELPNCGPAASIAAQGNFYGTDRSAEGIRL
HHHHHHCCCCCCCHHHEECCCCCCCCCCCCCCCC
>Mature Secondary Structure
MQPLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIQVDKCVENFVLVSRDTLS
CCCCCCCEEEEEECCEEEEEEECCCEEEECCCCEEEECCCHHHHHHHHHHHHHHCCHHHH
LFLPMLKEEALKLHAHKKVPSLLVHHCTRDIPVFQEVAQLSQNKNLRYAEMLRKRALIFA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
LLSVFLEDTQFIPLLLNVLQPNMRTRVCTVINNNIAHEWTLARIASELLMSPSLLKKKLR
HHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHHHHHHHH
EEGTSYSQLLTECRMQRALQLIVIYGVSIKRVAVSCGYHSVSYFIYVFRNYYGMTPTEYQ
HCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCHHH
ERSAQELPNCGPAASIAAQGNFYGTDRSAEGIRL
HHHHHHCCCCCCCHHHEECCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA