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The map label for this gene is gadW [H]

Identifier: 170680629

GI number: 170680629

Start: 3884535

End: 3885263

Strand: Reverse

Name: gadW [H]

Synonym: EcSMS35_3818

Alternate gene names: 170680629

Gene position: 3885263-3884535 (Counterclockwise)

Preceding gene: 170681182

Following gene: 170679753

Centisome position: 76.66

GC content: 41.7

Gene sequence:

>729_bases
ATGACTCATGTCTGCTCGGTGATCCTCGTTCGTCGTTCATTCGATATTTATCATGAACAGCAAAAAATATCGTTGCATAA
CGAGAGTATCCTGCTGCTGGATAAAAATTTGGCAGACGATTTTGCGTTTTGTTCACCGGATACGCGACGACTGGATATCG
ATGAGCTGACAGTTTGCCATTACTTACAAAATATTCGTCAGATACCACGCAATTTAGGGTTACATAGCAAAGACCGTCTG
TTAATTAACCAGTCACCCCCCATGCCGCTGGTGACGGCGATTTTTGATAGTTTCAATGACCCCCGGGTCAATTCGCCGAT
ACTGAGCAAAATGCTCTACCTTTCCTGTTTATCAATGTTTTCTCATAAGAAAGAACTGATCCCCTTACTTTTCAATAGTA
TCAGTACTGTTTCAGGAAAAGTTGAACGCCTTATTAGCTTTGATATCGCCAAACGTTGGTATCTGCGCGATATCGCAGAA
AGAATGTATACCAGCGAGAGTCTCATCAAAAAAAAGTTGCAGGATGAAAATACCTGTTTCAGTAAAATATTACTCGCCTC
CAGGATGTCGATGGCCAGACGATTACTCGAGTTACGTCAAATTCCTCTGCATACTATTGCGGAAAAATGTGGATATAGCA
GTACGTCGTACTTTATAAACACATTTCGACAATATTATGGTGTAACGCCACATCAGTTTGCGCAACATTCGCCAGGTACC
TTTTCCTGA

Upstream 100 bases:

>100_bases
TACCAAATCTGGCAGTTTTTGCGCTAAGAAACAGTCTGGCATCATTTCATTAGTATACTGAAATTGAAATAATCGCAGTA
ATGAAATATAAGGGATAGTC

Downstream 100 bases:

>100_bases
CATATTTTGCATTTGAATATTGGTCAGGATCTCACACCTGCTTCATGTGAAACTCTTCCCTGATGATTTCTGCCGGGCTA
CCGGCTAATTCTCTTTCGCA

Product: transcriptional regulator GadW

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 242; Mature: 241

Protein sequence:

>242_residues
MTHVCSVILVRRSFDIYHEQQKISLHNESILLLDKNLADDFAFCSPDTRRLDIDELTVCHYLQNIRQIPRNLGLHSKDRL
LINQSPPMPLVTAIFDSFNDPRVNSPILSKMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYFINTFRQYYGVTPHQFAQHSPGT
FS

Sequences:

>Translated_242_residues
MTHVCSVILVRRSFDIYHEQQKISLHNESILLLDKNLADDFAFCSPDTRRLDIDELTVCHYLQNIRQIPRNLGLHSKDRL
LINQSPPMPLVTAIFDSFNDPRVNSPILSKMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYFINTFRQYYGVTPHQFAQHSPGT
FS
>Mature_241_residues
THVCSVILVRRSFDIYHEQQKISLHNESILLLDKNLADDFAFCSPDTRRLDIDELTVCHYLQNIRQIPRNLGLHSKDRLL
INQSPPMPLVTAIFDSFNDPRVNSPILSKMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAER
MYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYFINTFRQYYGVTPHQFAQHSPGTF
S

Specific function: Depending on the conditions (growth phase and medium), acts as a positive or negative regulator of gadA and gadBC. Repression occurs directly or via the repression of the expression of gadX. Activation occurs directly by the binding of gadW to the gadA an

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789932, Length=242, Percent_Identity=97.1074380165289, Blast_Score=487, Evalue=1e-139,
Organism=Escherichia coli, GI1786776, Length=241, Percent_Identity=34.8547717842324, Blast_Score=165, Evalue=3e-42,
Organism=Escherichia coli, GI1787776, Length=237, Percent_Identity=34.1772151898734, Blast_Score=134, Evalue=5e-33,
Organism=Escherichia coli, GI1789933, Length=209, Percent_Identity=32.0574162679426, Blast_Score=95, Evalue=4e-21,
Organism=Escherichia coli, GI1790557, Length=136, Percent_Identity=36.7647058823529, Blast_Score=87, Evalue=7e-19,
Organism=Escherichia coli, GI1786778, Length=251, Percent_Identity=30.6772908366534, Blast_Score=85, Evalue=3e-18,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR018062
- InterPro:   IPR020449
- InterPro:   IPR018060 [H]

Pfam domain/function: PF00165 HTH_AraC [H]

EC number: NA

Molecular weight: Translated: 28110; Mature: 27979

Theoretical pI: Translated: 9.18; Mature: 9.18

Prosite motif: PS00041 HTH_ARAC_FAMILY_1 ; PS01124 HTH_ARAC_FAMILY_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTHVCSVILVRRSFDIYHEQQKISLHNESILLLDKNLADDFAFCSPDTRRLDIDELTVCH
CHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHCCCCCCCCCCHHHHHHHH
YLQNIRQIPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNDPRVNSPILSKMLYLSCLSMF
HHHHHHHHHHHCCCCCCCCEEEECCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHH
SHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAERMYTSESLIKKKLQDENTCF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
SKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYFINTFRQYYGVTPHQFAQHSPGT
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHCCCCCC
FS
CC
>Mature Secondary Structure 
THVCSVILVRRSFDIYHEQQKISLHNESILLLDKNLADDFAFCSPDTRRLDIDELTVCH
HHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHCCCCCCCCCCHHHHHHHH
YLQNIRQIPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNDPRVNSPILSKMLYLSCLSMF
HHHHHHHHHHHCCCCCCCCEEEECCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHH
SHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAERMYTSESLIKKKLQDENTCF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
SKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYFINTFRQYYGVTPHQFAQHSPGT
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHCCCCCC
FS
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]