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The map label for this gene is rph [H]

Identifier: 170680501

GI number: 170680501

Start: 4053855

End: 4054571

Strand: Reverse

Name: rph [H]

Synonym: EcSMS35_3978

Alternate gene names: 170680501

Gene position: 4054571-4053855 (Counterclockwise)

Preceding gene: 170681126

Following gene: 170679906

Centisome position: 80.0

GC content: 57.04

Gene sequence:

>717_bases
ATGCGTCCAGCAGGCCGTAGCAATAATCAGGTGCGTCCCGTTACCCTGACTCGTAACTATACAAAACATGCAGAAGGCTC
GGTGCTGGTCGAATTTGGCGATACCAAAGTATTGTGTACCGCCTCTATTGAAGAAGGCGTGCCGCGCTTCCTGAAAGGTC
AGGGCCAGGGCTGGATCACCGCAGAGTACGGCATGCTGCCACGTTCTACCCACACCCGTAACGCGCGTGAAGCAGCGAAA
GGTAAGCAGGGTGGGCGCACAATGGAAATCCAGCGTCTGATCGCCCGTGCTCTTCGCGCAGCAGTAGATTTGAAAGCGCT
GGGTGAGTTCACTATTACGCTGGACTGCGACGTGCTTCAGGCTGATGGTGGCACGCGTACCGCTTCGATTACGGGTGCCT
GCGTGGCGCTGGCAGATGCGTTACAGAAGCTGGTGGAAAACGGCAAGCTGAAAACCAACCCGATGAAAGGGATGGTAGCC
GCGGTTTCTGTCGGGATTGTGAACGGCGAAGCGGTTTGCGATCTGGAATACGTTGAAGACTCTGCCGCAGAGACCGACAT
GAACGTAGTGATGACCGAAGACGGGCGCATCATTGAAGTGCAGGGGACGGCAGAAGGCGAGCCGTTCACCCATGAAGAGC
TGCTCACCTTGTTGGCGCTGGCCCGAGGGGGAATCGAATCCATTGTAGCGACGCAGAAGGCGGCGCTGGCAAACTGA

Upstream 100 bases:

>100_bases
GTGGAAGGATTATAGCCATCGATGCCTTGTCAGGATAGGAATAACCGCCGGAAGTCCGTATAATGCGCAGCCACATTTGT
TTCAAGCCGGAGATTTCAAT

Downstream 100 bases:

>100_bases
TTTTTAAGGCGACTGATGAGTCGCCTTTTTTTTGTCTGTAGAAAAGTAAGATGAGGAGCGAAGGCATGAAACTATATCAG
CGCCAGTTTATTGAATTTGC

Product: ribonuclease PH

Products: NA

Alternate protein names: RNase PH; tRNA nucleotidyltransferase [H]

Number of amino acids: Translated: 238; Mature: 238

Protein sequence:

>238_residues
MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIEEGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAK
GKQGGRTMEIQRLIARALRAAVDLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
AVSVGIVNGEAVCDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLLALARGGIESIVATQKAALAN

Sequences:

>Translated_238_residues
MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIEEGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAK
GKQGGRTMEIQRLIARALRAAVDLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
AVSVGIVNGEAVCDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLLALARGGIESIVATQKAALAN
>Mature_238_residues
MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIEEGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAK
GKQGGRTMEIQRLIARALRAAVDLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
AVSVGIVNGEAVCDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLLALARGGIESIVATQKAALAN

Specific function: Phosphorolytic exoribonuclease that removes nucleotide residues following the -CCA terminus of tRNA and adds nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates [H]

COG id: COG0689

COG function: function code J; RNase PH

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase PH family [H]

Homologues:

Organism=Escherichia coli, GI157672248, Length=223, Percent_Identity=99.1031390134529, Blast_Score=452, Evalue=1e-129,
Organism=Caenorhabditis elegans, GI71981632, Length=189, Percent_Identity=28.042328042328, Blast_Score=70, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR020568
- InterPro:   IPR002381
- InterPro:   IPR018336 [H]

Pfam domain/function: PF01138 RNase_PH; PF03725 RNase_PH_C [H]

EC number: =2.7.7.56 [H]

Molecular weight: Translated: 25312; Mature: 25312

Theoretical pI: Translated: 5.42; Mature: 5.42

Prosite motif: PS01277 RIBONUCLEASE_PH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIEEGVPRFLKGQGQGWIT
CCCCCCCCCCEEEEEEECCCCCCCCCCEEEEECCCEEEEEECHHHCCHHHHCCCCCEEEE
AEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAVDLKALGEFTITLDCDVLQ
EECCCCCCCCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEEE
ADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVAAVSVGIVNGEAVCDLEYVED
CCCCCEEEEHHHHHHHHHHHHHHHHHCCCEECCCHHHEEEEEEEEEECCCEEEEHHHHCC
SAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLLALARGGIESIVATQKAALAN
CCCCCCCEEEEECCCEEEEEECCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIEEGVPRFLKGQGQGWIT
CCCCCCCCCCEEEEEEECCCCCCCCCCEEEEECCCEEEEEECHHHCCHHHHCCCCCEEEE
AEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAVDLKALGEFTITLDCDVLQ
EECCCCCCCCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEEE
ADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVAAVSVGIVNGEAVCDLEYVED
CCCCCEEEEHHHHHHHHHHHHHHHHHCCCEECCCHHHEEEEEEEEEECCCEEEEHHHHCC
SAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLLALARGGIESIVATQKAALAN
CCCCCCCEEEEECCCEEEEEECCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA