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The map label for this gene is nifS [H]

Identifier: 170077543

GI number: 170077543

Start: 952287

End: 953450

Strand: Reverse

Name: nifS [H]

Synonym: SYNPCC7002_A0921

Alternate gene names: 170077543

Gene position: 953450-952287 (Counterclockwise)

Preceding gene: 170077544

Following gene: 170077542

Centisome position: 31.7

GC content: 54.21

Gene sequence:

>1164_bases
ATGCAAATTTACCTTGATTACAGCGCCACAACGCCCCCCCATCCCTTGGTGATCCAGCGGGTGCAAGCAGTCTGTGGCCA
AACCTGGGGCAATCCCTCCAGCTTACATGGTTGGGGCAATCGGGCTGCTACCCTCCTGGAAATGGCGCGGATGCAAGTGG
CGAGCCTCATTGGTGCAGCGAACCCCGATGAAATTATTTTTACGGCAGGGGGTACCGAAGCCGATAACCTGGCAATTTTT
GGCATTACAGACCAGTACCGAGAGCCGCAACATTTGATTATCTCCCAGGTGGAACATCCGGCGATCGCCAAGGCCGCAGA
TTTCCTCGCAACCCAAGGCTGGCAAGTGACACGCCTCGGAGTTAATCGTCGTGGCCAAGTAGATCCTCAGGATCTAGAAA
AGGCGATTCAAGCCAACACGATTTTAATTTCGGTGATCTATGGCCAGAGCGAAGTGGGCACCATTCAACCCATTGCGGAA
CTGGGGGCGATCGCCAAAAAACATCACATTACCTTTCACACCGACGCAGTTCAGGCCGTGGGACGCATTCCCATTGATCT
CCGCCAGCTTCCCGTGGATTTGCTTTCCCTGTCTGGGCACAAAATCTATGGCCTCCAGGGGGCTGGTGCGTTGTATGTGC
GCGACGGTATTAAACTTCAGCCCCGACTCTACGGCGGCGGCCAAGAACACAATTTGCGTTCCGGCACCCAAAATTTACCC
GGCATCGTTGCTTTGGGGATGGCCGCTGAACTCGCCGAGGAAACCATGGCCCAAGAAATTGAACGCTTGAGTCGCCTCCG
CAATTTTTTGTTAAACCGAGGGAGCAGTAGCCCACATCTACAAATTACAGGCGATCGCCATGCCCGCTTACCCCACCATG
TCAGTTTTGCCTTGCGCCAAGATTCGCCCCTCCTGGGCCTAACCGGGAAAACCCTCGTGCGTCAAATGAACCTCGCTGGC
ATCGGTATTAGTGCGGGGTCTGCCTGCCATAGCGGTAAACTCAATCCCAGCACAACCCTGAAGGCGATGGGCTATAGCGA
TCAAGAGGCTCTCTCTGGGATTCGCTTAACCCTAGGTGCACAAACTCAAATGGAAGATATAGAATGGACGGCGATCGCCC
TCGAACAAATTTTGCAGCGACAACCTACCGTGCTTCTGGCATAA

Upstream 100 bases:

>100_bases
TGCGACTTTGGCTTATCCTGGGATACTCCCTTGGTCGCACGGGCTGACGGGTTGGCCCGAATTTGCGTATGCTAAGGGAA
CCATTGAGCGATCGCCCACC

Downstream 100 bases:

>100_bases
CCTGACCCAAATACATGGCTTCTGACTTCTCTAAGGATCCCTCCCCGCGGCTAGATCCAAGCCCTCCCATCCACCACAAA
CAAAATGAATTCTATGTGCT

Product: cysteine desulfurase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 387; Mature: 387

Protein sequence:

>387_residues
MQIYLDYSATTPPHPLVIQRVQAVCGQTWGNPSSLHGWGNRAATLLEMARMQVASLIGAANPDEIIFTAGGTEADNLAIF
GITDQYREPQHLIISQVEHPAIAKAADFLATQGWQVTRLGVNRRGQVDPQDLEKAIQANTILISVIYGQSEVGTIQPIAE
LGAIAKKHHITFHTDAVQAVGRIPIDLRQLPVDLLSLSGHKIYGLQGAGALYVRDGIKLQPRLYGGGQEHNLRSGTQNLP
GIVALGMAAELAEETMAQEIERLSRLRNFLLNRGSSSPHLQITGDRHARLPHHVSFALRQDSPLLGLTGKTLVRQMNLAG
IGISAGSACHSGKLNPSTTLKAMGYSDQEALSGIRLTLGAQTQMEDIEWTAIALEQILQRQPTVLLA

Sequences:

>Translated_387_residues
MQIYLDYSATTPPHPLVIQRVQAVCGQTWGNPSSLHGWGNRAATLLEMARMQVASLIGAANPDEIIFTAGGTEADNLAIF
GITDQYREPQHLIISQVEHPAIAKAADFLATQGWQVTRLGVNRRGQVDPQDLEKAIQANTILISVIYGQSEVGTIQPIAE
LGAIAKKHHITFHTDAVQAVGRIPIDLRQLPVDLLSLSGHKIYGLQGAGALYVRDGIKLQPRLYGGGQEHNLRSGTQNLP
GIVALGMAAELAEETMAQEIERLSRLRNFLLNRGSSSPHLQITGDRHARLPHHVSFALRQDSPLLGLTGKTLVRQMNLAG
IGISAGSACHSGKLNPSTTLKAMGYSDQEALSGIRLTLGAQTQMEDIEWTAIALEQILQRQPTVLLA
>Mature_387_residues
MQIYLDYSATTPPHPLVIQRVQAVCGQTWGNPSSLHGWGNRAATLLEMARMQVASLIGAANPDEIIFTAGGTEADNLAIF
GITDQYREPQHLIISQVEHPAIAKAADFLATQGWQVTRLGVNRRGQVDPQDLEKAIQANTILISVIYGQSEVGTIQPIAE
LGAIAKKHHITFHTDAVQAVGRIPIDLRQLPVDLLSLSGHKIYGLQGAGALYVRDGIKLQPRLYGGGQEHNLRSGTQNLP
GIVALGMAAELAEETMAQEIERLSRLRNFLLNRGSSSPHLQITGDRHARLPHHVSFALRQDSPLLGLTGKTLVRQMNLAG
IGISAGSACHSGKLNPSTTLKAMGYSDQEALSGIRLTLGAQTQMEDIEWTAIALEQILQRQPTVLLA

Specific function: Catalyzes the removal of elemental sulfur from cysteine to produce alanine [H]

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=385, Percent_Identity=40.5194805194805, Blast_Score=268, Evalue=6e-72,
Organism=Homo sapiens, GI156713448, Length=414, Percent_Identity=32.6086956521739, Blast_Score=193, Evalue=3e-49,
Organism=Escherichia coli, GI48994898, Length=376, Percent_Identity=42.0212765957447, Blast_Score=278, Evalue=6e-76,
Organism=Escherichia coli, GI1787970, Length=270, Percent_Identity=28.1481481481481, Blast_Score=91, Evalue=9e-20,
Organism=Escherichia coli, GI1789175, Length=355, Percent_Identity=26.4788732394366, Blast_Score=83, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI25143064, Length=377, Percent_Identity=39.7877984084881, Blast_Score=256, Evalue=1e-68,
Organism=Caenorhabditis elegans, GI17533177, Length=327, Percent_Identity=30.5810397553517, Blast_Score=140, Evalue=1e-33,
Organism=Saccharomyces cerevisiae, GI6319831, Length=378, Percent_Identity=39.9470899470899, Blast_Score=260, Evalue=3e-70,
Organism=Drosophila melanogaster, GI20129463, Length=376, Percent_Identity=40.6914893617021, Blast_Score=260, Evalue=1e-69,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR010240
- InterPro:   IPR017772
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 41836; Mature: 41836

Theoretical pI: Translated: 7.24; Mature: 7.24

Prosite motif: PS00595 AA_TRANSFER_CLASS_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQIYLDYSATTPPHPLVIQRVQAVCGQTWGNPSSLHGWGNRAATLLEMARMQVASLIGAA
CEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCC
NPDEIIFTAGGTEADNLAIFGITDQYREPQHLIISQVEHPAIAKAADFLATQGWQVTRLG
CCCCEEEECCCCCCCCEEEEECCHHCCCCHHHHHHHCCCCHHHHHHHHHHCCCCEEEEEC
VNRRGQVDPQDLEKAIQANTILISVIYGQSEVGTIQPIAELGAIAKKHHITFHTDAVQAV
CCCCCCCCHHHHHHHHHCCEEEEEEEECCCCCCCHHHHHHHHHHHHHCCEEEECHHHHHH
GRIPIDLRQLPVDLLSLSGHKIYGLQGAGALYVRDGIKLQPRLYGGGQEHNLRSGTQNLP
CCCCCCHHHCCHHHHHCCCCEEEEECCCCEEEEECCCEECEEEECCCCCCCCCCCCCCCC
GIVALGMAAELAEETMAQEIERLSRLRNFLLNRGSSSPHLQITGDRHARLPHHVSFALRQ
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCEEEEEEC
DSPLLGLTGKTLVRQMNLAGIGISAGSACHSGKLNPSTTLKAMGYSDQEALSGIRLTLGA
CCCEECCCHHHHHHHHCCCEEEECCCCCCCCCCCCCCCEEEECCCCCHHHHCCEEEEECC
QTQMEDIEWTAIALEQILQRQPTVLLA
CCCHHHHHHHHHHHHHHHHCCCCEEEC
>Mature Secondary Structure
MQIYLDYSATTPPHPLVIQRVQAVCGQTWGNPSSLHGWGNRAATLLEMARMQVASLIGAA
CEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCC
NPDEIIFTAGGTEADNLAIFGITDQYREPQHLIISQVEHPAIAKAADFLATQGWQVTRLG
CCCCEEEECCCCCCCCEEEEECCHHCCCCHHHHHHHCCCCHHHHHHHHHHCCCCEEEEEC
VNRRGQVDPQDLEKAIQANTILISVIYGQSEVGTIQPIAELGAIAKKHHITFHTDAVQAV
CCCCCCCCHHHHHHHHHCCEEEEEEEECCCCCCCHHHHHHHHHHHHHCCEEEECHHHHHH
GRIPIDLRQLPVDLLSLSGHKIYGLQGAGALYVRDGIKLQPRLYGGGQEHNLRSGTQNLP
CCCCCCHHHCCHHHHHCCCCEEEEECCCCEEEEECCCEECEEEECCCCCCCCCCCCCCCC
GIVALGMAAELAEETMAQEIERLSRLRNFLLNRGSSSPHLQITGDRHARLPHHVSFALRQ
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCEEEEEEC
DSPLLGLTGKTLVRQMNLAGIGISAGSACHSGKLNPSTTLKAMGYSDQEALSGIRLTLGA
CCCEECCCHHHHHHHHCCCEEEECCCCCCCCCCCCCCCEEEECCCCCHHHHCCEEEEECC
QTQMEDIEWTAIALEQILQRQPTVLLA
CCCHHHHHHHHHHHHHHHHCCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA