Click here to switch to the map view.

The map label for this gene is todF [H]

Identifier: 170077488

GI number: 170077488

Start: 893870

End: 894781

Strand: Reverse

Name: todF [H]

Synonym: SYNPCC7002_A0866

Alternate gene names: 170077488

Gene position: 894781-893870 (Counterclockwise)

Preceding gene: 170077493

Following gene: 170077480

Centisome position: 29.75

GC content: 50.66

Gene sequence:

>912_bases
ATGTATCCTTCCTTTTTACCGCCTCTGGTTAATCAGCTTACGGAACTTGATGCGATTCGCCTCGCCCAGCAGCTCCAACA
GGTGGCGATCGCCACTCCGCTTCAGACAAATCCCATCTTGACGAACTTTGTCCAACAGGGCAGCGGCGACCAAGACATTC
TCCTCATCCATGGCTTTGATAGCTCAGTTTTAGAATACCGCTATCTTTTGCCCAAATTAGCCCAGCAGCATCCTGTTTGG
GCCGTGGATTTGCTCAGTTTCGGTTTTACGGAGCGACCAGAACAGCTTCCCTTTACCCCAGAAACCATCAAAACCCACCT
CTGCCAGTTTTGGCAACAGCAAATTAATCGCCCGGTAGTGATTGTTGGTGCCTCTATGGGTGGGGCGGTGGCCCTCGAGT
TTGCCCTGAGCTACCCCGACGCGGTCAAGCAAATTGTCCTCTTAGATAGTGCTGGTCTGGCCCCAAAACCCCTCAGCCGC
TTTGCGATGGTACCGCCTTTGGATCGGTGGGCTACACAATTTTTAGGGAGTATGAATATCCGCCGAAAGATTTGCCAGAG
TGCCTATTTCGATAAAACAAAAGTCACAACAGATGCGGTACTCTGTGGCGCGATGCATGTCCAGTGCGATCGCTGGCAGG
AAGCCTTGATTCAATTTACGAAAGGCGGTGGCTATGGCTCTTTTTATCCCAAATTGAAGCAGATCCAGCAACCTACCTTA
ATTCTCTGGGGTGAACAAGACCGAATCCTTGGCACAAAAGCAGCCCATCGCTTTCAACAGGGCTTACCCAATAGCACCCT
CCACTGGATTCCCAATTGTGGCCACCTCCCCCATGTGGAGCAAACAACCCTCGTCGCCGAACACATTCTTCGCTTTTGCC
AAACTTTAATCCCAATCACATCTAATCATTAG

Upstream 100 bases:

>100_bases
CCCTAGCTCCCTAAATTTTCTCGGACACCGTTTGCTTGTAGGCCAGGGGCAAACCTGTAACAATAAGTAGGCAATTTGGC
TGCATTTGTTGAAATCCCCT

Downstream 100 bases:

>100_bases
CTTGAATCCTTGAGTTGATAGGAAATATTTTTTAATAAACTCAAGATGATATTTTTTGATTTTAATTAACTGGTGGAAGC
CGAAATATTGTTTTTACTGT

Product: hydrolase, alpha/beta fold family protein

Products: 2-oxopent-4-enoate; succinate [C]

Alternate protein names: NA

Number of amino acids: Translated: 303; Mature: 303

Protein sequence:

>303_residues
MYPSFLPPLVNQLTELDAIRLAQQLQQVAIATPLQTNPILTNFVQQGSGDQDILLIHGFDSSVLEYRYLLPKLAQQHPVW
AVDLLSFGFTERPEQLPFTPETIKTHLCQFWQQQINRPVVIVGASMGGAVALEFALSYPDAVKQIVLLDSAGLAPKPLSR
FAMVPPLDRWATQFLGSMNIRRKICQSAYFDKTKVTTDAVLCGAMHVQCDRWQEALIQFTKGGGYGSFYPKLKQIQQPTL
ILWGEQDRILGTKAAHRFQQGLPNSTLHWIPNCGHLPHVEQTTLVAEHILRFCQTLIPITSNH

Sequences:

>Translated_303_residues
MYPSFLPPLVNQLTELDAIRLAQQLQQVAIATPLQTNPILTNFVQQGSGDQDILLIHGFDSSVLEYRYLLPKLAQQHPVW
AVDLLSFGFTERPEQLPFTPETIKTHLCQFWQQQINRPVVIVGASMGGAVALEFALSYPDAVKQIVLLDSAGLAPKPLSR
FAMVPPLDRWATQFLGSMNIRRKICQSAYFDKTKVTTDAVLCGAMHVQCDRWQEALIQFTKGGGYGSFYPKLKQIQQPTL
ILWGEQDRILGTKAAHRFQQGLPNSTLHWIPNCGHLPHVEQTTLVAEHILRFCQTLIPITSNH
>Mature_303_residues
MYPSFLPPLVNQLTELDAIRLAQQLQQVAIATPLQTNPILTNFVQQGSGDQDILLIHGFDSSVLEYRYLLPKLAQQHPVW
AVDLLSFGFTERPEQLPFTPETIKTHLCQFWQQQINRPVVIVGASMGGAVALEFALSYPDAVKQIVLLDSAGLAPKPLSR
FAMVPPLDRWATQFLGSMNIRRKICQSAYFDKTKVTTDAVLCGAMHVQCDRWQEALIQFTKGGGYGSFYPKLKQIQQPTL
ILWGEQDRILGTKAAHRFQQGLPNSTLHWIPNCGHLPHVEQTTLVAEHILRFCQTLIPITSNH

Specific function: 3-hydroxyphenylpropionate degradation. [C]

COG id: COG0596

COG function: function code R; Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dmpD/todF/xylF esterase family [H]

Homologues:

Organism=Escherichia coli, GI87081721, Length=265, Percent_Identity=23.7735849056604, Blast_Score=67, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24586387, Length=128, Percent_Identity=29.6875, Blast_Score=66, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24586391, Length=128, Percent_Identity=29.6875, Blast_Score=66, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24586389, Length=128, Percent_Identity=29.6875, Blast_Score=66, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24586385, Length=128, Percent_Identity=29.6875, Blast_Score=66, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR000639 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: 3.7.1.- [C]

Molecular weight: Translated: 34048; Mature: 34048

Theoretical pI: Translated: 7.89; Mature: 7.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYPSFLPPLVNQLTELDAIRLAQQLQQVAIATPLQTNPILTNFVQQGSGDQDILLIHGFD
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCEEEEECCC
SSVLEYRYLLPKLAQQHPVWAVDLLSFGFTERPEQLPFTPETIKTHLCQFWQQQINRPVV
HHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEE
IVGASMGGAVALEFALSYPDAVKQIVLLDSAGLAPKPLSRFAMVPPLDRWATQFLGSMNI
EEECCCCCCEEHHHHHCCHHHHHHHHHCCCCCCCCCCHHHHCCCCCHHHHHHHHHHCHHH
RRKICQSAYFDKTKVTTDAVLCGAMHVQCDRWQEALIQFTKGGGYGSFYPKLKQIQQPTL
HHHHHHHHHCCCHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCEE
ILWGEQDRILGTKAAHRFQQGLPNSTLHWIPNCGHLPHVEQTTLVAEHILRFCQTLIPIT
EEECCCCCHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCC
SNH
CCC
>Mature Secondary Structure
MYPSFLPPLVNQLTELDAIRLAQQLQQVAIATPLQTNPILTNFVQQGSGDQDILLIHGFD
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCEEEEECCC
SSVLEYRYLLPKLAQQHPVWAVDLLSFGFTERPEQLPFTPETIKTHLCQFWQQQINRPVV
HHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEE
IVGASMGGAVALEFALSYPDAVKQIVLLDSAGLAPKPLSRFAMVPPLDRWATQFLGSMNI
EEECCCCCCEEHHHHHCCHHHHHHHHHCCCCCCCCCCHHHHCCCCCHHHHHHHHHHCHHH
RRKICQSAYFDKTKVTTDAVLCGAMHVQCDRWQEALIQFTKGGGYGSFYPKLKQIQQPTL
HHHHHHHHHCCCHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCEE
ILWGEQDRILGTKAAHRFQQGLPNSTLHWIPNCGHLPHVEQTTLVAEHILRFCQTLIPIT
EEECCCCCHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCC
SNH
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2-hydroxy-6-ketononadienedicarboxylate; H2O [C]

Specific reaction: 2-hydroxy-6-ketononadienedicarboxylate + H2O = 2-oxopent-4-enoate + succinate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9274030; 9384377 [H]