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The map label for this gene is metB [H]

Identifier: 170026302

GI number: 170026302

Start: 4526994

End: 4528235

Strand: Reverse

Name: metB [H]

Synonym: YPK_4095

Alternate gene names: 170026302

Gene position: 4528235-4526994 (Counterclockwise)

Preceding gene: 170026305

Following gene: 170026301

Centisome position: 96.56

GC content: 51.77

Gene sequence:

>1242_bases
ATGTCCAGATGTATTGACGTCCGTAATTTGTGCGTCTACTCTGGCGTAACATTTTCTCGATCCCGGCTGGTGGAAATCAA
TATGACGCGTAAACAGGCAACAATAGCAGTCCGTAGCGGGTTGAATGATGACGAGCAATACGGCTGCGTTGTCCCCCCGA
TTCACCTTTCCAGTACTTACAATTTTATTGATTTTAATCAGCCGCGCACGCATGACTATTCACGTCGTGGTAATCCAACG
CGTGATGTTGTCCAACGGGCGCTGGCGGAATTGGAAGGGGGGGCCGGTGCCGTCATGACCAGCAGCGGGATGTCGGCGCT
TCATTTGGTTTGCACTACATTCTTACAGCCGGGCGATCTGTTGGTCGCTCCGCATGACTGTTACGGTGGCAGTTACCGTT
TATTTGACAGCTTGAGCAAGCGTGGTGCGTATCGGGTGTTGTTTGTTGATCAGGGCGATGAAGCGGCACTGAACCGCGCA
TTGGCGGAGAAACCGAAGTTGGTCTTGATTGAAACACCGAGTAATCCATTGCTACGGGTTGTTGATATTGCCGCCATCTG
CCAAGCCGCCCGTGCTGCGGGCGCACTGACGGTTTGTGATAACACCTTCCTCAGCCCCGCCTTACAGCAGCCTCTCTCTC
TCGGGGCCGATTTAGTGGTGCACTCCTGTACCAAATATCTCAATGGTCACTCTGATGTGGTGGCTGGTGCTGTTATTGCG
AAAGATCCAGAACTGGTTGTTGAGCTGGCATGGTGGGCAAATAATATTGGTGTAACCGGTGCTGCGTTTGACAGCTATCT
ACTCCTTCGTGGTTTACGCACGTTATCACCACGCATGGCTCAACAGCAGCGTAACGCGGATGACATTGTGCGTTATTTAC
AGCAACAGCCTTTAGTGAAAAAGCTGTATCATCCTTCCCTGCCACAACATCCCGGCCACGAAATAGCCTGCCGTCAGCAA
TCAGGTTTTGGTGCAATGCTCAGTTTTGAGCTGGATGGTGATGAACAGGTCATGCGCCGTTTCCTTTCTGCCCTTGAGCT
ATTTACCTTGGCAGAGTCTTTGGGGGGGGTAGAAAGCCTGATCTCCCATGCAGCGACCATGACCCACGCGGGTATGGCGG
CAGAGGCGCGTATTGCCGCAGGCATTACTGATAGTTTGTTGCGTATTTCCGTGGGTATTGAAGACAGTGAAGATTTGATT
GCTGATTTGGACCACGCGTTCCAATTGGCAGTAACGAGGTAA

Upstream 100 bases:

>100_bases
CGCTAATGACGTTGGATATAAAAACCTACTTTAAATACAAAGCCTCTATAATTTTAGCGGTTATTATTCCATTGCTCAAT
CTATACGCAAAGAAGTTTAG

Downstream 100 bases:

>100_bases
GCATGAATGCAACAGCGGTAGCAGCGGCGGCAACGGGCCGTCAACTGCATAAATTTGGTGGCAGTAGCCTTGCGGATGTG
AAGTGTTATCTACGGGTGGC

Product: cystathionine gamma-synthase

Products: NA

Alternate protein names: CGS; O-succinylhomoserine (thiol)-lyase [H]

Number of amino acids: Translated: 413; Mature: 412

Protein sequence:

>413_residues
MSRCIDVRNLCVYSGVTFSRSRLVEINMTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFIDFNQPRTHDYSRRGNPT
RDVVQRALAELEGGAGAVMTSSGMSALHLVCTTFLQPGDLLVAPHDCYGGSYRLFDSLSKRGAYRVLFVDQGDEAALNRA
LAEKPKLVLIETPSNPLLRVVDIAAICQAARAAGALTVCDNTFLSPALQQPLSLGADLVVHSCTKYLNGHSDVVAGAVIA
KDPELVVELAWWANNIGVTGAAFDSYLLLRGLRTLSPRMAQQQRNADDIVRYLQQQPLVKKLYHPSLPQHPGHEIACRQQ
SGFGAMLSFELDGDEQVMRRFLSALELFTLAESLGGVESLISHAATMTHAGMAAEARIAAGITDSLLRISVGIEDSEDLI
ADLDHAFQLAVTR

Sequences:

>Translated_413_residues
MSRCIDVRNLCVYSGVTFSRSRLVEINMTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFIDFNQPRTHDYSRRGNPT
RDVVQRALAELEGGAGAVMTSSGMSALHLVCTTFLQPGDLLVAPHDCYGGSYRLFDSLSKRGAYRVLFVDQGDEAALNRA
LAEKPKLVLIETPSNPLLRVVDIAAICQAARAAGALTVCDNTFLSPALQQPLSLGADLVVHSCTKYLNGHSDVVAGAVIA
KDPELVVELAWWANNIGVTGAAFDSYLLLRGLRTLSPRMAQQQRNADDIVRYLQQQPLVKKLYHPSLPQHPGHEIACRQQ
SGFGAMLSFELDGDEQVMRRFLSALELFTLAESLGGVESLISHAATMTHAGMAAEARIAAGITDSLLRISVGIEDSEDLI
ADLDHAFQLAVTR
>Mature_412_residues
SRCIDVRNLCVYSGVTFSRSRLVEINMTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFIDFNQPRTHDYSRRGNPTR
DVVQRALAELEGGAGAVMTSSGMSALHLVCTTFLQPGDLLVAPHDCYGGSYRLFDSLSKRGAYRVLFVDQGDEAALNRAL
AEKPKLVLIETPSNPLLRVVDIAAICQAARAAGALTVCDNTFLSPALQQPLSLGADLVVHSCTKYLNGHSDVVAGAVIAK
DPELVVELAWWANNIGVTGAAFDSYLLLRGLRTLSPRMAQQQRNADDIVRYLQQQPLVKKLYHPSLPQHPGHEIACRQQS
GFGAMLSFELDGDEQVMRRFLSALELFTLAESLGGVESLISHAATMTHAGMAAEARIAAGITDSLLRISVGIEDSEDLIA
DLDHAFQLAVTR

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=363, Percent_Identity=41.0468319559229, Blast_Score=264, Evalue=1e-70,
Organism=Homo sapiens, GI299473759, Length=363, Percent_Identity=38.5674931129477, Blast_Score=233, Evalue=3e-61,
Organism=Homo sapiens, GI25453487, Length=362, Percent_Identity=36.7403314917127, Blast_Score=209, Evalue=3e-54,
Organism=Escherichia coli, GI1790375, Length=383, Percent_Identity=83.289817232376, Blast_Score=667, Evalue=0.0,
Organism=Escherichia coli, GI1789383, Length=342, Percent_Identity=33.0409356725146, Blast_Score=160, Evalue=1e-40,
Organism=Caenorhabditis elegans, GI17538055, Length=385, Percent_Identity=47.012987012987, Blast_Score=311, Evalue=4e-85,
Organism=Caenorhabditis elegans, GI17560056, Length=383, Percent_Identity=45.4308093994778, Blast_Score=295, Evalue=4e-80,
Organism=Caenorhabditis elegans, GI71980708, Length=369, Percent_Identity=34.4173441734417, Blast_Score=221, Evalue=4e-58,
Organism=Saccharomyces cerevisiae, GI6319307, Length=390, Percent_Identity=39.7435897435897, Blast_Score=257, Evalue=2e-69,
Organism=Saccharomyces cerevisiae, GI6321254, Length=400, Percent_Identity=34.25, Blast_Score=195, Evalue=1e-50,
Organism=Saccharomyces cerevisiae, GI6321904, Length=394, Percent_Identity=29.9492385786802, Blast_Score=148, Evalue=1e-36,
Organism=Saccharomyces cerevisiae, GI6323333, Length=381, Percent_Identity=31.496062992126, Blast_Score=145, Evalue=1e-35,
Organism=Saccharomyces cerevisiae, GI6321170, Length=342, Percent_Identity=28.3625730994152, Blast_Score=144, Evalue=3e-35,
Organism=Saccharomyces cerevisiae, GI6322590, Length=283, Percent_Identity=27.208480565371, Blast_Score=76, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6322970, Length=271, Percent_Identity=25.830258302583, Blast_Score=66, Evalue=1e-11,
Organism=Drosophila melanogaster, GI19922554, Length=381, Percent_Identity=42.5196850393701, Blast_Score=306, Evalue=1e-83,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR011821
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =2.5.1.48 [H]

Molecular weight: Translated: 44892; Mature: 44761

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRCIDVRNLCVYSGVTFSRSRLVEINMTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTY
CCCCHHHHHHHHHCCCEECCCEEEEEEECHHHHHHHHHCCCCCCCCCCCCCCCEECCCCC
NFIDFNQPRTHDYSRRGNPTRDVVQRALAELEGGAGAVMTSSGMSALHLVCTTFLQPGDL
CEEECCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHCCCCCE
LVAPHDCYGGSYRLFDSLSKRGAYRVLFVDQGDEAALNRALAEKPKLVLIETPSNPLLRV
EEECCCCCCCCHHHHHHHHHCCCEEEEEEECCCHHHHHHHHHCCCCEEEEECCCCCHHHH
VDIAAICQAARAAGALTVCDNTFLSPALQQPLSLGADLVVHSCTKYLNGHSDVVAGAVIA
HHHHHHHHHHHHCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCEEE
KDPELVVELAWWANNIGVTGAAFDSYLLLRGLRTLSPRMAQQQRNADDIVRYLQQQPLVK
CCCHHEEEEEEHHCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHCCHHHHHHHHHHCCHHH
KLYHPSLPQHPGHEIACRQQSGFGAMLSFELDGDEQVMRRFLSALELFTLAESLGGVESL
HHCCCCCCCCCCCCEEEECCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCHHHH
ISHAATMTHAGMAAEARIAAGITDSLLRISVGIEDSEDLIADLDHAFQLAVTR
HHHHHHHHHCCCHHHHHHHHCCHHCEEEEEECCCCCHHHHHHHHHHHEEEECC
>Mature Secondary Structure 
SRCIDVRNLCVYSGVTFSRSRLVEINMTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTY
CCCHHHHHHHHHCCCEECCCEEEEEEECHHHHHHHHHCCCCCCCCCCCCCCCEECCCCC
NFIDFNQPRTHDYSRRGNPTRDVVQRALAELEGGAGAVMTSSGMSALHLVCTTFLQPGDL
CEEECCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHCCCCCE
LVAPHDCYGGSYRLFDSLSKRGAYRVLFVDQGDEAALNRALAEKPKLVLIETPSNPLLRV
EEECCCCCCCCHHHHHHHHHCCCEEEEEEECCCHHHHHHHHHCCCCEEEEECCCCCHHHH
VDIAAICQAARAAGALTVCDNTFLSPALQQPLSLGADLVVHSCTKYLNGHSDVVAGAVIA
HHHHHHHHHHHHCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCEEE
KDPELVVELAWWANNIGVTGAAFDSYLLLRGLRTLSPRMAQQQRNADDIVRYLQQQPLVK
CCCHHEEEEEEHHCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHCCHHHHHHHHHHCCHHH
KLYHPSLPQHPGHEIACRQQSGFGAMLSFELDGDEQVMRRFLSALELFTLAESLGGVESL
HHCCCCCCCCCCCCEEEECCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCHHHH
ISHAATMTHAGMAAEARIAAGITDSLLRISVGIEDSEDLIADLDHAFQLAVTR
HHHHHHHHHCCCHHHHHHHHCCHHCEEEEEECCCCCHHHHHHHHHHHEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6361020; 8346018; 9278503; 3307782; 9843488 [H]