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The map label for this gene is lptD [H]

Identifier: 170025785

GI number: 170025785

Start: 3933665

End: 3935917

Strand: Direct

Name: lptD [H]

Synonym: YPK_3570

Alternate gene names: 170025785

Gene position: 3933665-3935917 (Clockwise)

Preceding gene: 170025783

Following gene: 170025786

Centisome position: 83.88

GC content: 47.89

Gene sequence:

>2253_bases
ATGCTCGGTGTCCCAACCTATGATCAACCACTGGTCACAGGCGATCCGAATCAACTGCCGGTCCGTATTAATGCGGATAA
AACCGAAGCAAACTATCCGGATAATGCCTTGTTTACTGGCAATGTTATTGTTCAGCAAGGCAATAGCACCCTAACAGCGA
ACCAAGTGGAACTGACACAGGTACAGAAACCCGGTGAAGTCATTCCCCTTCGTACCGTTACCGCCACCGGCGATGTCAAC
TACGACGATCCGCAAATCAAGCTGAAAGGACCAAAAGGTTGGTCAAATCTGAACACCAAAGATACTGATATAGACAAAGG
TAAATATCAGATGGTGGGCCGTCAGGGCCGTGGTGATGCAGATTTGATGAAATTGCGCGATCAGAGCCGCTACACCATTT
TGAAAAACGGCACATTTACCTCTTGCCTACCCGGAGATAACAGTTGGAGCGTGGTTGGCTCAGAGGTCATTCATGACCGC
GAAGAACAAGTGGTTGAAGTTTGGAATGCTCGTTTCAAGATTGGCAAAGTCCCGGTGTTTTACAGCCCTTATATGCAATT
GCCAGTTGGCGATAAACGCCGTTCGGGGTTCTTGATCCCAAATGCGAAATTTACCAGTAACAATGGTTTTGAGTTTTTAC
TCCCTTATTACTGGAATATTGCCCCCAACTTTGATGCCACCATTACGCCACACTATATGGAAAGGCGCGGGTTGCAATGG
CAAAACGAATTCCGCTATTTGCTGGCGCCGGGTTCAGGCACCATGGCATTAGACTGGTTGCCCAATGACCGTATCTATAC
GGGGCCTGATGGCACAGATAAAAATGCCACCCGCTGGCTATACTACTGGGGCCATTCCGGTGTAATGGATCAAGTTTGGC
GCTTCAACATCAACTATACCCGTGTCAGCGACCCCGCTTACTTTACGGATTTAACCTCTCAGTACGGTTCGACAACCGAT
GGCTACGCGACACAAATATTCACCGCCGGTTATGCCAATGAAAACTGGAATGCGACGCTGTCTTCCAAGCAGTTCCAGGT
ATTTACTGCTGCAGGTAACAGTAATGCCTACCGTGCCCAGCCACAGTTGGACATGAACTACTACAAAAACGATGTTGGTC
CATTTGATATGCATGTCTATGGTCAGGCGGCCAAATTCACCAGTGTCAATCCAACTAACCCGGAAGCCAGTCGTTTCCAT
ATTGAACCTACGGTTAACCTGCCCTTATCCAATAGCTGGGGAAGCATAAATACAGAAGCCAAACTGTTGGCCACCCACTA
TCAACAGGATATTCCGGCCAGTTTTGCTGACAATGCTAGCAACCCCAAACTGAAAGACTCAGTAAACCGGGTCCTACCAC
AGTTTAAAGTGGATGGTAAGGTGGTATTCGATAGGTCAATGGACTGGGCGACGGGCTTTACGCAGACATTGGAGCCTCGC
GCACAGTATCTCTACGTCCCTTACCGGAACCAAGATGATATCTATATATATGACACCACGTTGATGCAGTCGGATTACTC
TGGTCTGTTCCGTGATCGTACCTATAGTGGCTTGGACCGTATTGCATCGGCTAATCAGGTATCTACCGGTTTAACCTCTC
GCATATATGATGATGCTCGGGTTGAACGTTTTAATGTTTCTGTAGGTCAAATCTATTACTTCAGTCGATCACGCACAGGT
AATACCGAAGCCATCGATAACAGTAACGATACAGGGAGCCTGGTTTGGGCTGGTGATACTTTTTGGCGGATCAACGATCA
ACTGGGCCTGAAGGGCGGCGCACAGTATGATACTCGGCTGGGAAGTTTGACCTTAGGTAACGCTATAATGGAATATAGAA
GAGACGCTGATCGTATGATTCAATTGAATTACCGCTACGCGAGCCCAAAATATATTCAGGCGGCTGTCCCTAAAGTATAC
AACCCTGACTACCAGCAAGGCATCTCTCAGGTAGGTACCACGGCCAGTTGGCCAATTGCTGACCGCTGGGCGATCGTCGG
GGCGTATTATTACGATACCAAAGCCAAACAACCTGCCAGCCAGTTAGTTGGCTTGCAATACAACACCTGTTGTTGGGCGG
TAAACCTTGGCTATGAACGCAAAATAACTGGCTGGAATGCACAAGGCCAAACCAGTAAGTACGACAACAAAATAGGCTTT
AACATCGAATTACGTGGTTTGAGCGGTGGTCATAGCCTTGGTACTGCTCAAATGCTGAACTCGGGCATTCTGCCTTATCA
AAGTGCATTCTGA

Upstream 100 bases:

>100_bases
AAAACCACGTATGAAAAAAAGATTCCCAACACTGCTGGCCACATTGATTTGGACGGCACTATATAGTCAACACACACTGG
CAGACCTTGCGGAGCAATGT

Downstream 100 bases:

>100_bases
TACTGTTTAACACACTGAATACAGTGAATCTTAACCCGCGATAGCGGATTACATAAATGGAAAAGGTATGAAGAACTGGA
GAACGCTTATTCTCGGATTG

Product: organic solvent tolerance protein

Products: NA

Alternate protein names: Organic solvent tolerance protein [H]

Number of amino acids: Translated: 750; Mature: 750

Protein sequence:

>750_residues
MLGVPTYDQPLVTGDPNQLPVRINADKTEANYPDNALFTGNVIVQQGNSTLTANQVELTQVQKPGEVIPLRTVTATGDVN
YDDPQIKLKGPKGWSNLNTKDTDIDKGKYQMVGRQGRGDADLMKLRDQSRYTILKNGTFTSCLPGDNSWSVVGSEVIHDR
EEQVVEVWNARFKIGKVPVFYSPYMQLPVGDKRRSGFLIPNAKFTSNNGFEFLLPYYWNIAPNFDATITPHYMERRGLQW
QNEFRYLLAPGSGTMALDWLPNDRIYTGPDGTDKNATRWLYYWGHSGVMDQVWRFNINYTRVSDPAYFTDLTSQYGSTTD
GYATQIFTAGYANENWNATLSSKQFQVFTAAGNSNAYRAQPQLDMNYYKNDVGPFDMHVYGQAAKFTSVNPTNPEASRFH
IEPTVNLPLSNSWGSINTEAKLLATHYQQDIPASFADNASNPKLKDSVNRVLPQFKVDGKVVFDRSMDWATGFTQTLEPR
AQYLYVPYRNQDDIYIYDTTLMQSDYSGLFRDRTYSGLDRIASANQVSTGLTSRIYDDARVERFNVSVGQIYYFSRSRTG
NTEAIDNSNDTGSLVWAGDTFWRINDQLGLKGGAQYDTRLGSLTLGNAIMEYRRDADRMIQLNYRYASPKYIQAAVPKVY
NPDYQQGISQVGTTASWPIADRWAIVGAYYYDTKAKQPASQLVGLQYNTCCWAVNLGYERKITGWNAQGQTSKYDNKIGF
NIELRGLSGGHSLGTAQMLNSGILPYQSAF

Sequences:

>Translated_750_residues
MLGVPTYDQPLVTGDPNQLPVRINADKTEANYPDNALFTGNVIVQQGNSTLTANQVELTQVQKPGEVIPLRTVTATGDVN
YDDPQIKLKGPKGWSNLNTKDTDIDKGKYQMVGRQGRGDADLMKLRDQSRYTILKNGTFTSCLPGDNSWSVVGSEVIHDR
EEQVVEVWNARFKIGKVPVFYSPYMQLPVGDKRRSGFLIPNAKFTSNNGFEFLLPYYWNIAPNFDATITPHYMERRGLQW
QNEFRYLLAPGSGTMALDWLPNDRIYTGPDGTDKNATRWLYYWGHSGVMDQVWRFNINYTRVSDPAYFTDLTSQYGSTTD
GYATQIFTAGYANENWNATLSSKQFQVFTAAGNSNAYRAQPQLDMNYYKNDVGPFDMHVYGQAAKFTSVNPTNPEASRFH
IEPTVNLPLSNSWGSINTEAKLLATHYQQDIPASFADNASNPKLKDSVNRVLPQFKVDGKVVFDRSMDWATGFTQTLEPR
AQYLYVPYRNQDDIYIYDTTLMQSDYSGLFRDRTYSGLDRIASANQVSTGLTSRIYDDARVERFNVSVGQIYYFSRSRTG
NTEAIDNSNDTGSLVWAGDTFWRINDQLGLKGGAQYDTRLGSLTLGNAIMEYRRDADRMIQLNYRYASPKYIQAAVPKVY
NPDYQQGISQVGTTASWPIADRWAIVGAYYYDTKAKQPASQLVGLQYNTCCWAVNLGYERKITGWNAQGQTSKYDNKIGF
NIELRGLSGGHSLGTAQMLNSGILPYQSAF
>Mature_750_residues
MLGVPTYDQPLVTGDPNQLPVRINADKTEANYPDNALFTGNVIVQQGNSTLTANQVELTQVQKPGEVIPLRTVTATGDVN
YDDPQIKLKGPKGWSNLNTKDTDIDKGKYQMVGRQGRGDADLMKLRDQSRYTILKNGTFTSCLPGDNSWSVVGSEVIHDR
EEQVVEVWNARFKIGKVPVFYSPYMQLPVGDKRRSGFLIPNAKFTSNNGFEFLLPYYWNIAPNFDATITPHYMERRGLQW
QNEFRYLLAPGSGTMALDWLPNDRIYTGPDGTDKNATRWLYYWGHSGVMDQVWRFNINYTRVSDPAYFTDLTSQYGSTTD
GYATQIFTAGYANENWNATLSSKQFQVFTAAGNSNAYRAQPQLDMNYYKNDVGPFDMHVYGQAAKFTSVNPTNPEASRFH
IEPTVNLPLSNSWGSINTEAKLLATHYQQDIPASFADNASNPKLKDSVNRVLPQFKVDGKVVFDRSMDWATGFTQTLEPR
AQYLYVPYRNQDDIYIYDTTLMQSDYSGLFRDRTYSGLDRIASANQVSTGLTSRIYDDARVERFNVSVGQIYYFSRSRTG
NTEAIDNSNDTGSLVWAGDTFWRINDQLGLKGGAQYDTRLGSLTLGNAIMEYRRDADRMIQLNYRYASPKYIQAAVPKVY
NPDYQQGISQVGTTASWPIADRWAIVGAYYYDTKAKQPASQLVGLQYNTCCWAVNLGYERKITGWNAQGQTSKYDNKIGF
NIELRGLSGGHSLGTAQMLNSGILPYQSAF

Specific function: Together with lptE, is involved in the assembly of LPS in the outer leaflet of the outer membrane. Determines N-hexane tolerance and is involved in outer membrane permeability. Essential for envelope biogenesis [H]

COG id: COG1452

COG function: function code M; Organic solvent tolerance protein OstA

Gene ontology:

Cell location: Cell outer membrane [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lptD family [H]

Homologues:

Organism=Escherichia coli, GI1786239, Length=755, Percent_Identity=66.2251655629139, Blast_Score=1060, Evalue=0.0,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020889
- InterPro:   IPR005653
- InterPro:   IPR007543 [H]

Pfam domain/function: PF03968 OstA; PF04453 OstA_C [H]

EC number: NA

Molecular weight: Translated: 84518; Mature: 84518

Theoretical pI: Translated: 6.89; Mature: 6.89

Prosite motif: PS00435 PEROXIDASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLGVPTYDQPLVTGDPNQLPVRINADKTEANYPDNALFTGNVIVQQGNSTLTANQVELTQ
CCCCCCCCCCEECCCCCEEEEEEECCCCCCCCCCCEEEECEEEEECCCCEEEECEEEEEE
VQKPGEVIPLRTVTATGDVNYDDPQIKLKGPKGWSNLNTKDTDIDKGKYQMVGRQGRGDA
ECCCCCEEEEEEEEECCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCEEEECCCCCCCH
DLMKLRDQSRYTILKNGTFTSCLPGDNSWSVVGSEVIHDREEQVVEVWNARFKIGKVPVF
HHHHHCCCCCEEEEECCCEEEECCCCCCCEEECHHHHHCHHHHHHHHHCCEEEECCCEEE
YSPYMQLPVGDKRRSGFLIPNAKFTSNNGFEFLLPYYWNIAPNFDATITPHYMERRGLQW
ECCEEECCCCCCCCCCEECCCCEEECCCCCEEEEEEEEEECCCCCCEECCHHHHHCCCCC
QNEFRYLLAPGSGTMALDWLPNDRIYTGPDGTDKNATRWLYYWGHSGVMDQVWRFNINYT
CCCCEEEEECCCCCEEEEECCCCCEEECCCCCCCCCEEEEEEECCCCHHHHHEEEEEEEE
RVSDPAYFTDLTSQYGSTTDGYATQIFTAGYANENWNATLSSKQFQVFTAAGNSNAYRAQ
EECCCHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCEEECCCEEEEEEEECCCCCEECC
PQLDMNYYKNDVGPFDMHVYGQAAKFTSVNPTNPEASRFHIEPTVNLPLSNSWGSINTEA
CCCCHHHHHCCCCCEEEEEEECEEEECCCCCCCCCCCEEEECEEEECCCCCCCCCCCCCH
KLLATHYQQDIPASFADNASNPKLKDSVNRVLPQFKVDGKVVFDRSMDWATGFTQTLEPR
HHHHHHHHHCCCCHHCCCCCCCCHHHHHHHHCCCEECCCEEEEECCCCCCCCCHHHCCCC
AQYLYVPYRNQDDIYIYDTTLMQSDYSGLFRDRTYSGLDRIASANQVSTGLTSRIYDDAR
CCEEEEEECCCCCEEEEEEEEECCCCCHHHHCCCHHHHHHHHCCCHHHHCHHHHHHCCCC
VERFNVSVGQIYYFSRSRTGNTEAIDNSNDTGSLVWAGDTFWRINDQLGLKGGAQYDTRL
EEEEECEEEEEEEEECCCCCCCCCCCCCCCCCEEEECCCEEEEECCCCCCCCCCCCCCCC
GSLTLGNAIMEYRRDADRMIQLNYRYASPKYIQAAVPKVYNPDYQQGISQVGTTASWPIA
CCEEHHHHHHHHHCCCCEEEEEEEEECCCCEEECCCCCCCCCCHHHHHHHHCCCCCCCCC
DRWAIVGAYYYDTKAKQPASQLVGLQYNTCCWAVNLGYERKITGWNAQGQTSKYDNKIGF
CCEEEEEEEEECCCCCCCHHHHCCCEECCEEEEEECCCEEEECCCCCCCCCCCCCCEECE
NIELRGLSGGHSLGTAQMLNSGILPYQSAF
EEEEEECCCCCCCCHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MLGVPTYDQPLVTGDPNQLPVRINADKTEANYPDNALFTGNVIVQQGNSTLTANQVELTQ
CCCCCCCCCCEECCCCCEEEEEEECCCCCCCCCCCEEEECEEEEECCCCEEEECEEEEEE
VQKPGEVIPLRTVTATGDVNYDDPQIKLKGPKGWSNLNTKDTDIDKGKYQMVGRQGRGDA
ECCCCCEEEEEEEEECCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCEEEECCCCCCCH
DLMKLRDQSRYTILKNGTFTSCLPGDNSWSVVGSEVIHDREEQVVEVWNARFKIGKVPVF
HHHHHCCCCCEEEEECCCEEEECCCCCCCEEECHHHHHCHHHHHHHHHCCEEEECCCEEE
YSPYMQLPVGDKRRSGFLIPNAKFTSNNGFEFLLPYYWNIAPNFDATITPHYMERRGLQW
ECCEEECCCCCCCCCCEECCCCEEECCCCCEEEEEEEEEECCCCCCEECCHHHHHCCCCC
QNEFRYLLAPGSGTMALDWLPNDRIYTGPDGTDKNATRWLYYWGHSGVMDQVWRFNINYT
CCCCEEEEECCCCCEEEEECCCCCEEECCCCCCCCCEEEEEEECCCCHHHHHEEEEEEEE
RVSDPAYFTDLTSQYGSTTDGYATQIFTAGYANENWNATLSSKQFQVFTAAGNSNAYRAQ
EECCCHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCEEECCCEEEEEEEECCCCCEECC
PQLDMNYYKNDVGPFDMHVYGQAAKFTSVNPTNPEASRFHIEPTVNLPLSNSWGSINTEA
CCCCHHHHHCCCCCEEEEEEECEEEECCCCCCCCCCCEEEECEEEECCCCCCCCCCCCCH
KLLATHYQQDIPASFADNASNPKLKDSVNRVLPQFKVDGKVVFDRSMDWATGFTQTLEPR
HHHHHHHHHCCCCHHCCCCCCCCHHHHHHHHCCCEECCCEEEEECCCCCCCCCHHHCCCC
AQYLYVPYRNQDDIYIYDTTLMQSDYSGLFRDRTYSGLDRIASANQVSTGLTSRIYDDAR
CCEEEEEECCCCCEEEEEEEEECCCCCHHHHCCCHHHHHHHHCCCHHHHCHHHHHHCCCC
VERFNVSVGQIYYFSRSRTGNTEAIDNSNDTGSLVWAGDTFWRINDQLGLKGGAQYDTRL
EEEEECEEEEEEEEECCCCCCCCCCCCCCCCCEEEECCCEEEEECCCCCCCCCCCCCCCC
GSLTLGNAIMEYRRDADRMIQLNYRYASPKYIQAAVPKVYNPDYQQGISQVGTTASWPIA
CCEEHHHHHHHHHCCCCEEEEEEEEECCCCEEECCCCCCCCCCHHHHHHHHCCCCCCCCC
DRWAIVGAYYYDTKAKQPASQLVGLQYNTCCWAVNLGYERKITGWNAQGQTSKYDNKIGF
CCEEEEEEEEECCCCCCCHHHHCCCEECCEEEEEECCCEEEECCCCCCCCCCCCCCEECE
NIELRGLSGGHSLGTAQMLNSGILPYQSAF
EEEEEECCCCCCCCHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA