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The map label for this gene is clpB [H]

Identifier: 170025564

GI number: 170025564

Start: 3674077

End: 3676650

Strand: Reverse

Name: clpB [H]

Synonym: YPK_3349

Alternate gene names: 170025564

Gene position: 3676650-3674077 (Counterclockwise)

Preceding gene: 170025565

Following gene: 170025557

Centisome position: 78.4

GC content: 47.98

Gene sequence:

>2574_bases
ATGCGTCTGGATCGTCTTACCAGTAAATTCCAGCTTGCCCTCGCCGATGCCCAGTCTTTAGCTCTTGGGCGCGACAACCA
GTTTATTGAACCGTTACATTTGATGAGCGCACTGCTTAATCAGGATGGGGGGACGGTTCGCCCTTTATTGACCTCGGCAG
GGATTAATGTTGCACGTTTACGTAGCGACATTGAGCAAGCTTTAGGTCGTCTTCCTCAAGTTGAAGGCACCGGCGGTGAT
GTTCAACCATCAAACGAGCTGGTTCGTGTATTAAATCTTTGCGACAAATTAGCGCAGAAACGTTCGGATAAGTTTATCTC
TTCCGAGCTGTTCGTCCTGGCCGTACTTGAAGATCGTGGCACGTTAACTGACATGTTAAAGGCTGCAGGTGCAACCGTCG
CTATAATAGATAAGGCAATTGAAACCATGCGTGGTGGTGACAAAGTCGATGATCAGGGTGCTGAAGATCAGCGCCAGGCG
TTGAAAAAATTCACTATCGACCTGACTGAGCGTGCCGAGCAAGGCAAACTCGATCCGGTTATTGGCCGTGATGAAGAAAT
TCGCCGTACTATTCAGGTATTACAACGTCGAACCAAAAATAACCCAGTGCTGATTGGTGAGCCTGGGGTGGGTAAAACCG
CTATTGTGGAAGGTTTGGCGCAACGCATTGTGAACGGCGAAGTGCCAGAAGGCTTGAAGCATAAGCGTGTACTCTCATTA
GATATGGGGTCGCTGATTGCCGGTGCCAAATATCGTGGTGAATTTGAAGAGCGTTTGAAAGGCGTACTGAACGACTTATC
AAAACAAGAAGGCAGTGTGATCTTGTTTATTGATGAGTTGCATACCATGGTCGGTGCCGGTAAAGGCGATGGGGCGATGG
ATGCCGGTAACATGCTGAAACCTGCATTAGCGCGAGGTGAGTTACATTGTGTGGGGGCCACTACCCTCGATGAATACCGT
CAATATATAGAGAAAGATGCTGCGCTCGAGCGGCGTTTCCAAAAAGTGTATGTTGCGGAACCAACGGTAGAAGACACTAT
CGCTATTTTACGTGGGCTGAAAGAGCGCTACGAACTGCACCATCACGTGCAGATCACTGACCCAGCTATTGTGGCAGCCG
CAACATTGTCACACCGGTATATTTCTGATCGTCAGTTACCGGATAAAGCTATCGACCTCATTGATGAGGCAGGTTCCAGC
ATCCGTATGCAAATGGATTCAAAACCCGAATCATTGGATCGTCTGGAGCGCCGGATTATTCAGCTTAAACTGGAACAGCA
GGCCCTAAAAAAAGAGTCAGATGACGCCAGTAAAAAACGGTTGGAAATGCTCAATACTGAGTTAGAGCAAAAAGAGCGTG
AATATTCTGAACTGGAAGAAGAGTGGAAAGCAGAAAAAGCTTCACTCACCGGGACTCAGAATATCAAGACGGAGTTGGAA
CAAGCTAAAATCACCTTAGAACAAGCTCGTCGAGTAGGTGATCTGGCTCGGATGTCAGAACTTCAGTACGGTACAATCCC
TGAGCTGGAAAAACAGCTGGCTGCGGCAACCGCGCTAGAAGGGAAAACCATGAAGTTGCTGCGTAACCGTGTCACAGAAA
TCGAAGTTGCTGAAGTCCTGGCTCGCTGGACCGGTATCCCGGTATCTCGCATGTTGGAAAGTGAGCGGGATAAGCTGCTA
CGTATGGAACAGGACTTGCATCAACGAGTCATTGGGCAAGATGAAGCTGTTGAAGCCGTCTCCAACGCTATCCGTCGGAG
TCGCGCAGGGTTGTCTGATCCAAACCGACCAATTGGTTCGTTCTTATTCTTAGGGCCAACGGGGGTTGGTAAAACTGAAT
TGTGTAAAGCGTTGGCAACGTTCTTGTTCGACAGTGACGATGCCATGGTGCGAATCGACATGTCCGAGTTTATGGAGAAA
CATTCCGTATCGCGGTTGGTCGGTGCACCTCCTGGCTATGTTGGGTATGAAGAAGGTGGCTATCTGACGGAAGCGGTTCG
TCGTCGTCCTTATTCTGTGATTTTGCTGGATGAAGTTGAAAAAGCACATCCAGATGTTTTCAACATATTGTTACAGGTAT
TGGATGATGGTCGTTTGACCGACGGGCAGGGGAGAACTGTCGACTTTCGTAACACGGTAGTCATTATGACCTCTAACCTT
GGTTCAGATTTGATTCAGGAACATTTCGGCCAAATGAATTACAGCGAAATGAAGACAGTGGTTATGGATGTTGTTAGTCA
CCACTTCCGTCCTGAATTTATTAACCGTATAGATGAGGTTGTTGTCTTCCACCCACTTGGTCGGGCGCACTTGGCTTCTA
TTGCCAACATTCAACTGGAGCGTTTGTATAAACGTCTGGAAGAACGTGGCTATGAAGTGAAGATTACTCAGCCTGCATTG
GAATTCTTGGGTGAAGCTGGCTTCGACCCTGTTTATGGGGCGCGGCCATTAAAACGTGCAATTCAGCAGGAAATTGAGAA
CCCGCTGGCACAGCAGATACTTTCTGGCAAACTAATACCGGGAATCCCCGTGACTTTAGATGTTGAAAATGGGCATATTA
TTGCTAAGCAGTAA

Upstream 100 bases:

>100_bases
AGAACGCATTATTTTAATATAGTGTCAAAATAACCTTGAAAAATTGAGGGATGACCTCATTTAATCTCCAGTAGCAATTT
TGACCAATATTGGAGGTGTT

Downstream 100 bases:

>100_bases
TAGCTGATCCTCTAAGGGGCGAGAAATCGCCCCTTTTTTGTTGTTTTTTTCTTCATATATTGCATTTATGATGCATTAGT
GAGCGGTTGAAAAGTTTTTT

Product: protein disaggregation chaperone

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 857; Mature: 857

Protein sequence:

>857_residues
MRLDRLTSKFQLALADAQSLALGRDNQFIEPLHLMSALLNQDGGTVRPLLTSAGINVARLRSDIEQALGRLPQVEGTGGD
VQPSNELVRVLNLCDKLAQKRSDKFISSELFVLAVLEDRGTLTDMLKAAGATVAIIDKAIETMRGGDKVDDQGAEDQRQA
LKKFTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEGLAQRIVNGEVPEGLKHKRVLSL
DMGSLIAGAKYRGEFEERLKGVLNDLSKQEGSVILFIDELHTMVGAGKGDGAMDAGNMLKPALARGELHCVGATTLDEYR
QYIEKDAALERRFQKVYVAEPTVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYISDRQLPDKAIDLIDEAGSS
IRMQMDSKPESLDRLERRIIQLKLEQQALKKESDDASKKRLEMLNTELEQKEREYSELEEEWKAEKASLTGTQNIKTELE
QAKITLEQARRVGDLARMSELQYGTIPELEKQLAAATALEGKTMKLLRNRVTEIEVAEVLARWTGIPVSRMLESERDKLL
RMEQDLHQRVIGQDEAVEAVSNAIRRSRAGLSDPNRPIGSFLFLGPTGVGKTELCKALATFLFDSDDAMVRIDMSEFMEK
HSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKAHPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNL
GSDLIQEHFGQMNYSEMKTVVMDVVSHHFRPEFINRIDEVVVFHPLGRAHLASIANIQLERLYKRLEERGYEVKITQPAL
EFLGEAGFDPVYGARPLKRAIQQEIENPLAQQILSGKLIPGIPVTLDVENGHIIAKQ

Sequences:

>Translated_857_residues
MRLDRLTSKFQLALADAQSLALGRDNQFIEPLHLMSALLNQDGGTVRPLLTSAGINVARLRSDIEQALGRLPQVEGTGGD
VQPSNELVRVLNLCDKLAQKRSDKFISSELFVLAVLEDRGTLTDMLKAAGATVAIIDKAIETMRGGDKVDDQGAEDQRQA
LKKFTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEGLAQRIVNGEVPEGLKHKRVLSL
DMGSLIAGAKYRGEFEERLKGVLNDLSKQEGSVILFIDELHTMVGAGKGDGAMDAGNMLKPALARGELHCVGATTLDEYR
QYIEKDAALERRFQKVYVAEPTVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYISDRQLPDKAIDLIDEAGSS
IRMQMDSKPESLDRLERRIIQLKLEQQALKKESDDASKKRLEMLNTELEQKEREYSELEEEWKAEKASLTGTQNIKTELE
QAKITLEQARRVGDLARMSELQYGTIPELEKQLAAATALEGKTMKLLRNRVTEIEVAEVLARWTGIPVSRMLESERDKLL
RMEQDLHQRVIGQDEAVEAVSNAIRRSRAGLSDPNRPIGSFLFLGPTGVGKTELCKALATFLFDSDDAMVRIDMSEFMEK
HSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKAHPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNL
GSDLIQEHFGQMNYSEMKTVVMDVVSHHFRPEFINRIDEVVVFHPLGRAHLASIANIQLERLYKRLEERGYEVKITQPAL
EFLGEAGFDPVYGARPLKRAIQQEIENPLAQQILSGKLIPGIPVTLDVENGHIIAKQ
>Mature_857_residues
MRLDRLTSKFQLALADAQSLALGRDNQFIEPLHLMSALLNQDGGTVRPLLTSAGINVARLRSDIEQALGRLPQVEGTGGD
VQPSNELVRVLNLCDKLAQKRSDKFISSELFVLAVLEDRGTLTDMLKAAGATVAIIDKAIETMRGGDKVDDQGAEDQRQA
LKKFTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEGLAQRIVNGEVPEGLKHKRVLSL
DMGSLIAGAKYRGEFEERLKGVLNDLSKQEGSVILFIDELHTMVGAGKGDGAMDAGNMLKPALARGELHCVGATTLDEYR
QYIEKDAALERRFQKVYVAEPTVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYISDRQLPDKAIDLIDEAGSS
IRMQMDSKPESLDRLERRIIQLKLEQQALKKESDDASKKRLEMLNTELEQKEREYSELEEEWKAEKASLTGTQNIKTELE
QAKITLEQARRVGDLARMSELQYGTIPELEKQLAAATALEGKTMKLLRNRVTEIEVAEVLARWTGIPVSRMLESERDKLL
RMEQDLHQRVIGQDEAVEAVSNAIRRSRAGLSDPNRPIGSFLFLGPTGVGKTELCKALATFLFDSDDAMVRIDMSEFMEK
HSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKAHPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNL
GSDLIQEHFGQMNYSEMKTVVMDVVSHHFRPEFINRIDEVVVFHPLGRAHLASIANIQLERLYKRLEERGYEVKITQPAL
EFLGEAGFDPVYGARPLKRAIQQEIENPLAQQILSGKLIPGIPVTLDVENGHIIAKQ

Specific function: Part of a stress-induced multi-chaperone system, it is involved in the recovery of the cell from heat-induced damage, in cooperation with DnaK, DnaJ and GrpE. Acts before DnaK, in the processing of protein aggregates. Protein binding stimulates the ATPase

COG id: COG0542

COG function: function code O; ATPases with chaperone activity, ATP-binding subunit

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the clpA/clpB family [H]

Homologues:

Organism=Homo sapiens, GI13540606, Length=320, Percent_Identity=37.1875, Blast_Score=218, Evalue=2e-56,
Organism=Escherichia coli, GI1788943, Length=857, Percent_Identity=86.6977829638273, Blast_Score=1526, Evalue=0.0,
Organism=Escherichia coli, GI1787109, Length=419, Percent_Identity=39.8568019093079, Blast_Score=280, Evalue=2e-76,
Organism=Saccharomyces cerevisiae, GI6320464, Length=710, Percent_Identity=52.9577464788732, Blast_Score=761, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6323002, Length=838, Percent_Identity=43.1980906921241, Blast_Score=694, Evalue=0.0,

Paralogues:

None

Copy number: 560 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013093
- InterPro:   IPR003959
- InterPro:   IPR018368
- InterPro:   IPR017730
- InterPro:   IPR001270
- InterPro:   IPR019489
- InterPro:   IPR004176
- InterPro:   IPR023150 [H]

Pfam domain/function: PF00004 AAA; PF07724 AAA_2; PF02861 Clp_N; PF10431 ClpB_D2-small [H]

EC number: NA

Molecular weight: Translated: 95647; Mature: 95647

Theoretical pI: Translated: 5.42; Mature: 5.42

Prosite motif: PS00870 CLPAB_1 ; PS00871 CLPAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLDRLTSKFQLALADAQSLALGRDNQFIEPLHLMSALLNQDGGTVRPLLTSAGINVARL
CCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCEEHHHHHHCCCHHHHH
RSDIEQALGRLPQVEGTGGDVQPSNELVRVLNLCDKLAQKRSDKFISSELFVLAVLEDRG
HHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCC
TLTDMLKAAGATVAIIDKAIETMRGGDKVDDQGAEDQRQALKKFTIDLTERAEQGKLDPV
HHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCC
IGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEGLAQRIVNGEVPEGLKHKRVLSL
CCCCHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEEE
DMGSLIAGAKYRGEFEERLKGVLNDLSKQEGSVILFIDELHTMVGAGKGDGAMDAGNMLK
CHHHHHHCCHHCCCHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHCCCCCCCCCCCCHHHH
PALARGELHCVGATTLDEYRQYIEKDAALERRFQKVYVAEPTVEDTIAILRGLKERYELH
HHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
HHVQITDPAIVAAATLSHRYISDRQLPDKAIDLIDEAGSSIRMQMDSKPESLDRLERRII
HCEEECCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHH
QLKLEQQALKKESDDASKKRLEMLNTELEQKEREYSELEEEWKAEKASLTGTQNIKTELE
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
QAKITLEQARRVGDLARMSELQYGTIPELEKQLAAATALEGKTMKLLRNRVTEIEVAEVL
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
ARWTGIPVSRMLESERDKLLRMEQDLHQRVIGQDEAVEAVSNAIRRSRAGLSDPNRPIGS
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCC
FLFLGPTGVGKTELCKALATFLFDSDDAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGG
EEEECCCCCCHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHHHHHCCCCCCCCCCCCC
YLTEAVRRRPYSVILLDEVEKAHPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNL
HHHHHHHCCCCEEEEECCHHHHCCHHHHHHHHHHCCCCCCCCCCCEEEECCEEEEEECCC
GSDLIQEHFGQMNYSEMKTVVMDVVSHHFRPEFINRIDEVVVFHPLGRAHLASIANIQLE
CHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHEEEECCCCHHHHHHHHHHHHH
RLYKRLEERGYEVKITQPALEFLGEAGFDPVYGARPLKRAIQQEIENPLAQQILSGKLIP
HHHHHHHHCCCEEEEEHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCC
GIPVTLDVENGHIIAKQ
CCCEEEEECCCEEEECC
>Mature Secondary Structure
MRLDRLTSKFQLALADAQSLALGRDNQFIEPLHLMSALLNQDGGTVRPLLTSAGINVARL
CCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCEEHHHHHHCCCHHHHH
RSDIEQALGRLPQVEGTGGDVQPSNELVRVLNLCDKLAQKRSDKFISSELFVLAVLEDRG
HHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCC
TLTDMLKAAGATVAIIDKAIETMRGGDKVDDQGAEDQRQALKKFTIDLTERAEQGKLDPV
HHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCC
IGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEGLAQRIVNGEVPEGLKHKRVLSL
CCCCHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEEE
DMGSLIAGAKYRGEFEERLKGVLNDLSKQEGSVILFIDELHTMVGAGKGDGAMDAGNMLK
CHHHHHHCCHHCCCHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHCCCCCCCCCCCCHHHH
PALARGELHCVGATTLDEYRQYIEKDAALERRFQKVYVAEPTVEDTIAILRGLKERYELH
HHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
HHVQITDPAIVAAATLSHRYISDRQLPDKAIDLIDEAGSSIRMQMDSKPESLDRLERRII
HCEEECCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHH
QLKLEQQALKKESDDASKKRLEMLNTELEQKEREYSELEEEWKAEKASLTGTQNIKTELE
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
QAKITLEQARRVGDLARMSELQYGTIPELEKQLAAATALEGKTMKLLRNRVTEIEVAEVL
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
ARWTGIPVSRMLESERDKLLRMEQDLHQRVIGQDEAVEAVSNAIRRSRAGLSDPNRPIGS
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCC
FLFLGPTGVGKTELCKALATFLFDSDDAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGG
EEEECCCCCCHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHHHHHCCCCCCCCCCCCC
YLTEAVRRRPYSVILLDEVEKAHPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNL
HHHHHHHCCCCEEEEECCHHHHCCHHHHHHHHHHCCCCCCCCCCCEEEECCEEEEEECCC
GSDLIQEHFGQMNYSEMKTVVMDVVSHHFRPEFINRIDEVVVFHPLGRAHLASIANIQLE
CHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHEEEECCCCHHHHHHHHHHHHH
RLYKRLEERGYEVKITQPALEFLGEAGFDPVYGARPLKRAIQQEIENPLAQQILSGKLIP
HHHHHHHHCCCEEEEEHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCC
GIPVTLDVENGHIIAKQ
CCCEEEEECCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases); Serine endopeptidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430 [H]