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The map label for this gene is yfiF [H]

Identifier: 170025557

GI number: 170025557

Start: 3660524

End: 3661690

Strand: Reverse

Name: yfiF [H]

Synonym: YPK_3340

Alternate gene names: 170025557

Gene position: 3661690-3660524 (Counterclockwise)

Preceding gene: 170025564

Following gene: 170025547

Centisome position: 78.08

GC content: 53.21

Gene sequence:

>1167_bases
ATGAACGATTCATTTAGTGGCAAAAACGGCAAAGTTAAAGTGATGTACGTCCGTAGTGACGAAAGTAGCGACGACCGTAA
CAGCAGTAACCGCGATAACCGCAATAACAGTAGTCGTGATAACAGTAATCGTGATAATAACAGCCGCGGCAGAAGCCAGC
GTCCAGCGGGTAACGGTCGTCCGGGTGATAATGCACGTCAGGGCGATAATACACGTCAGGGTAATGCACCACGCAATAAT
GACTCACGTCGTCCTGATTCTGGCCGTAATGAACGTGATCGTCCTAGCCGTCCGGCACGTTCTGAAGGCAGTAGCCCTTA
CGATTCACCGTGGAAAACGGTATCTCGTGCCCCTTCGGAAGAGCCAGAGTTTGATCATGGTGGTATCAGCGGTAAAAGCC
ATATCGATCCTGCCCAGTTGCGCCGTCAGCGCGCTGAAGAAACACGCGTTTATGGTGAGAATGCTTGTAAGGCGCTGTTT
GAAAGCCGCCCTGATGCTATCGTCCGGGCTTGGTTTGTGCAATCTGTGACCCCGCGTTTTCGTGAAGCGCTGAAATGGAT
GGCGGCTAACCGCAAAGCTTATCATGTGGTTGAAGAGGATGAGCTGGCGAAAGCTTCGGGCACTGAACACCATGGCGGGG
TATGTTTCCTGATTAAAAAACGTCAGGGGCTGGATGCAGAAACGTATCTGCAACAGCAAACTCAGGCTAAAGATTGTGTA
CTGGCGCTGGAAGAGGTGGGTAACCCCCATAATTTGGGCGGCATTATGCGTACCTGTGCGCATTTCGGTATCAACGGCGT
GTTGCTGCAAGACCCGGCGATGCTAGAATCTGGCGCGGCAGTCCGCACGGCGGAAGGCGGAGCAGAGCACATCAAGGCCA
TCAATGCTGATGATTTCTTATCGGTATTGGATACCTTCCGCAAGGCGGGTTACACCATCGTGACCACCTCCAGCCATAAA
GGTGTCAGCTTATCGAAAGCCGAACTGCCAGCCAAAATGGTATTGGTATTAGGGCAGGAGAGCGATGGTTTGACGGACAG
TGCCTGGCAGCAGGGGGATCTGAGTGTCTCTATCGGCGGTACCGGGCGGGTCGAAAGCCTGAACGTTTCCGTGGCAACGG
GTATCCTGTTGGCGGAATGGTGGCGTCAGAATAGCGCTCAAGGCTAG

Upstream 100 bases:

>100_bases
ATTCGATGAGTACGATAGTTGGTAGCTTTTAGTGCTTGCTTATGGCTAACCGGGCGGACATCAGGTAATCTTCGCGCCCT
GCGCGTTGGTGGAGAAGACA

Downstream 100 bases:

>100_bases
AATGATCGAAGAGTAGAACGAGAGACGTTTTAGAACGGTAAAAATAAAAGGGGGCGCTGATGAGTTCAGAGCCCCCTTTT
TTGTTGACCGCTATTTGGCT

Product: putative methyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 388; Mature: 388

Protein sequence:

>388_residues
MNDSFSGKNGKVKVMYVRSDESSDDRNSSNRDNRNNSSRDNSNRDNNSRGRSQRPAGNGRPGDNARQGDNTRQGNAPRNN
DSRRPDSGRNERDRPSRPARSEGSSPYDSPWKTVSRAPSEEPEFDHGGISGKSHIDPAQLRRQRAEETRVYGENACKALF
ESRPDAIVRAWFVQSVTPRFREALKWMAANRKAYHVVEEDELAKASGTEHHGGVCFLIKKRQGLDAETYLQQQTQAKDCV
LALEEVGNPHNLGGIMRTCAHFGINGVLLQDPAMLESGAAVRTAEGGAEHIKAINADDFLSVLDTFRKAGYTIVTTSSHK
GVSLSKAELPAKMVLVLGQESDGLTDSAWQQGDLSVSIGGTGRVESLNVSVATGILLAEWWRQNSAQG

Sequences:

>Translated_388_residues
MNDSFSGKNGKVKVMYVRSDESSDDRNSSNRDNRNNSSRDNSNRDNNSRGRSQRPAGNGRPGDNARQGDNTRQGNAPRNN
DSRRPDSGRNERDRPSRPARSEGSSPYDSPWKTVSRAPSEEPEFDHGGISGKSHIDPAQLRRQRAEETRVYGENACKALF
ESRPDAIVRAWFVQSVTPRFREALKWMAANRKAYHVVEEDELAKASGTEHHGGVCFLIKKRQGLDAETYLQQQTQAKDCV
LALEEVGNPHNLGGIMRTCAHFGINGVLLQDPAMLESGAAVRTAEGGAEHIKAINADDFLSVLDTFRKAGYTIVTTSSHK
GVSLSKAELPAKMVLVLGQESDGLTDSAWQQGDLSVSIGGTGRVESLNVSVATGILLAEWWRQNSAQG
>Mature_388_residues
MNDSFSGKNGKVKVMYVRSDESSDDRNSSNRDNRNNSSRDNSNRDNNSRGRSQRPAGNGRPGDNARQGDNTRQGNAPRNN
DSRRPDSGRNERDRPSRPARSEGSSPYDSPWKTVSRAPSEEPEFDHGGISGKSHIDPAQLRRQRAEETRVYGENACKALF
ESRPDAIVRAWFVQSVTPRFREALKWMAANRKAYHVVEEDELAKASGTEHHGGVCFLIKKRQGLDAETYLQQQTQAKDCV
LALEEVGNPHNLGGIMRTCAHFGINGVLLQDPAMLESGAAVRTAEGGAEHIKAINADDFLSVLDTFRKAGYTIVTTSSHK
GVSLSKAELPAKMVLVLGQESDGLTDSAWQQGDLSVSIGGTGRVESLNVSVATGILLAEWWRQNSAQG

Specific function: Unknown

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1788935, Length=388, Percent_Identity=60.0515463917526, Blast_Score=447, Evalue=1e-127,
Organism=Escherichia coli, GI1790623, Length=246, Percent_Identity=26.8292682926829, Blast_Score=76, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001537
- InterPro:   IPR013123
- InterPro:   IPR016479 [H]

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 42489; Mature: 42489

Theoretical pI: Translated: 8.33; Mature: 8.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDSFSGKNGKVKVMYVRSDESSDDRNSSNRDNRNNSSRDNSNRDNNSRGRSQRPAGNGR
CCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
PGDNARQGDNTRQGNAPRNNDSRRPDSGRNERDRPSRPARSEGSSPYDSPWKTVSRAPSE
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCC
EPEFDHGGISGKSHIDPAQLRRQRAEETRVYGENACKALFESRPDAIVRAWFVQSVTPRF
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCHHH
REALKWMAANRKAYHVVEEDELAKASGTEHHGGVCFLIKKRQGLDAETYLQQQTQAKDCV
HHHHHHHHCCCCEEEEECHHHHHHHCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHH
LALEEVGNPHNLGGIMRTCAHFGINGVLLQDPAMLESGAAVRTAEGGAEHIKAINADDFL
HHHHHCCCCCHHHHHHHHHHHHCCCCEEECCCHHHHCCCEEEECCCCHHHHEECCHHHHH
SVLDTFRKAGYTIVTTSSHKGVSLSKAELPAKMVLVLGQESDGLTDSAWQQGDLSVSIGG
HHHHHHHHCCCEEEEECCCCCCCCHHHHCCEEEEEEECCCCCCCCHHHHCCCCEEEEECC
TGRVESLNVSVATGILLAEWWRQNSAQG
CCCEEEEEEHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MNDSFSGKNGKVKVMYVRSDESSDDRNSSNRDNRNNSSRDNSNRDNNSRGRSQRPAGNGR
CCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
PGDNARQGDNTRQGNAPRNNDSRRPDSGRNERDRPSRPARSEGSSPYDSPWKTVSRAPSE
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCC
EPEFDHGGISGKSHIDPAQLRRQRAEETRVYGENACKALFESRPDAIVRAWFVQSVTPRF
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCHHH
REALKWMAANRKAYHVVEEDELAKASGTEHHGGVCFLIKKRQGLDAETYLQQQTQAKDCV
HHHHHHHHCCCCEEEEECHHHHHHHCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHH
LALEEVGNPHNLGGIMRTCAHFGINGVLLQDPAMLESGAAVRTAEGGAEHIKAINADDFL
HHHHHCCCCCHHHHHHHHHHHHCCCCEEECCCHHHHCCCEEEECCCCHHHHEECCHHHHH
SVLDTFRKAGYTIVTTSSHKGVSLSKAELPAKMVLVLGQESDGLTDSAWQQGDLSVSIGG
HHHHHHHHCCCEEEEECCCCCCCCHHHHCCEEEEEEECCCCCCCCHHHHCCCCEEEEECC
TGRVESLNVSVATGILLAEWWRQNSAQG
CCCEEEEEEHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]