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The map label for this gene is rfbS [H]

Identifier: 170025405

GI number: 170025405

Start: 3486687

End: 3487544

Strand: Reverse

Name: rfbS [H]

Synonym: YPK_3188

Alternate gene names: 170025405

Gene position: 3487544-3486687 (Counterclockwise)

Preceding gene: 170025406

Following gene: 170025404

Centisome position: 74.37

GC content: 35.66

Gene sequence:

>858_bases
ATGAAAATTCTTATTACCGGCGTTAGCGGTTATTTGGGGAGCCAATTAGCCAATGCTTTAATGCTAGAGCATGAAGTTGC
GGGAACTGTGCGTGCTGGTTCTGTATGTAATCGCATCACTGATATTGGTAATGTTAATTTAATTAATGTTACTGACAGTG
GCTGGATAGATAAGGTTTTGTCATTTTCTCCTGATGTGGTTATTAATACTGTAGCATTATATGGAAGGAAAGGAGAGTTA
CTTTCAGAGCTGGTTGATGCCAATATTCAATTTCCATTACGTATATTAGAAATGTTAGTTTCAACAGGAAAAGGCTTGTT
TTTTCAGTGCGGAACATCTCTACCTGCGGATGTTAGTCAATACGCTTTAACTAAAAATCAATTCACTGAGCTTGCCAGAG
AATATTGCAATAAATTTAGTGGTAAATTTATAGAGTTAAAATTAGAACATTTTTTTGGTCCTTTTGATGATTCAACTAAA
TTTACAACTTATGTAATCAATAGTTGTAGAAGTCATAGTGACCTAAAATTGACAGCCGGCTTACAACGCCGTGATTTTAT
TTATATTAATGATCTTATCAACGCTTTCAAAATTATGATTTCAAAATCAGAGAGTCTAATTTCAGGCGAAAGTATTTCCA
TTGGGTCCGGTCATGCTGTCACGATAAAAGAATTTGTAGAAACTGTCGCAAAGATGACAAGTTACCAAGGCAATCTACAG
TTTGGTGCAATCCCTACTAGAGAGAATGAATTGATGTATAGCTGTGCATCATTGGCGAGAATACAGGAACTCGGTTGGTT
GTGCCAATATTCATTAAATAGTGCAATTAAAGATACACTAAACAGAATGCGGGTCTGA

Upstream 100 bases:

>100_bases
GACACATGATCATTTGGATTATGTTGTGAGTAAGCTTGAAGAGTTCTTTGGTTTGAATTTTTAACAGGAGCAAGGAGGCA
ACACTGCCTCTTTTTTTTAT

Downstream 100 bases:

>100_bases
ATGGATATGAAATTTATAATCAAGATGGCTATTTTAAGTGCGAATCTTAGATAGAGTGAAGTAAATATATAAATACAGAT
TTGATTCAATAAGCCAATTG

Product: NAD-dependent epimerase/dehydratase

Products: GDP-4-dehydro-6-L-deoxygalactose; guanosine diphosphate fucose; NADP [C]

Alternate protein names: NA

Number of amino acids: Translated: 285; Mature: 285

Protein sequence:

>285_residues
MKILITGVSGYLGSQLANALMLEHEVAGTVRAGSVCNRITDIGNVNLINVTDSGWIDKVLSFSPDVVINTVALYGRKGEL
LSELVDANIQFPLRILEMLVSTGKGLFFQCGTSLPADVSQYALTKNQFTELAREYCNKFSGKFIELKLEHFFGPFDDSTK
FTTYVINSCRSHSDLKLTAGLQRRDFIYINDLINAFKIMISKSESLISGESISIGSGHAVTIKEFVETVAKMTSYQGNLQ
FGAIPTRENELMYSCASLARIQELGWLCQYSLNSAIKDTLNRMRV

Sequences:

>Translated_285_residues
MKILITGVSGYLGSQLANALMLEHEVAGTVRAGSVCNRITDIGNVNLINVTDSGWIDKVLSFSPDVVINTVALYGRKGEL
LSELVDANIQFPLRILEMLVSTGKGLFFQCGTSLPADVSQYALTKNQFTELAREYCNKFSGKFIELKLEHFFGPFDDSTK
FTTYVINSCRSHSDLKLTAGLQRRDFIYINDLINAFKIMISKSESLISGESISIGSGHAVTIKEFVETVAKMTSYQGNLQ
FGAIPTRENELMYSCASLARIQELGWLCQYSLNSAIKDTLNRMRV
>Mature_285_residues
MKILITGVSGYLGSQLANALMLEHEVAGTVRAGSVCNRITDIGNVNLINVTDSGWIDKVLSFSPDVVINTVALYGRKGEL
LSELVDANIQFPLRILEMLVSTGKGLFFQCGTSLPADVSQYALTKNQFTELAREYCNKFSGKFIELKLEHFFGPFDDSTK
FTTYVINSCRSHSDLKLTAGLQRRDFIYINDLINAFKIMISKSESLISGESISIGSGHAVTIKEFVETVAKMTSYQGNLQ
FGAIPTRENELMYSCASLARIQELGWLCQYSLNSAIKDTLNRMRV

Specific function: Two Step Nadp-Dependent Conversion Of Gdp-4-Dehydro-6- Deoxy-D-Mannose To Gdp-Fucose, Involving An Epimerase And A Reductase Reaction. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: 1.1.1.271 [C]

Molecular weight: Translated: 31575; Mature: 31575

Theoretical pI: Translated: 6.90; Mature: 6.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILITGVSGYLGSQLANALMLEHEVAGTVRAGSVCNRITDIGNVNLINVTDSGWIDKVL
CEEEEEECCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCEEEEEECCCHHHHHHH
SFSPDVVINTVALYGRKGELLSELVDANIQFPLRILEMLVSTGKGLFFQCGTSLPADVSQ
HCCCCHHHHHHHHHCCCHHHHHHHHCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCHHHH
YALTKNQFTELAREYCNKFSGKFIELKLEHFFGPFDDSTKFTTYVINSCRSHSDLKLTAG
HHHHHHHHHHHHHHHHHHHCCCEEEEEHHHCCCCCCCCCHHHHHHHHHHCCCCCEEEECC
LQRRDFIYINDLINAFKIMISKSESLISGESISIGSGHAVTIKEFVETVAKMTSYQGNLQ
CCCCCEEEHHHHHHHHHHHHHCCCHHCCCCCEECCCCCEEEHHHHHHHHHHHHCCCCCEE
FGAIPTRENELMYSCASLARIQELGWLCQYSLNSAIKDTLNRMRV
ECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKILITGVSGYLGSQLANALMLEHEVAGTVRAGSVCNRITDIGNVNLINVTDSGWIDKVL
CEEEEEECCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCEEEEEECCCHHHHHHH
SFSPDVVINTVALYGRKGELLSELVDANIQFPLRILEMLVSTGKGLFFQCGTSLPADVSQ
HCCCCHHHHHHHHHCCCHHHHHHHHCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCHHHH
YALTKNQFTELAREYCNKFSGKFIELKLEHFFGPFDDSTKFTTYVINSCRSHSDLKLTAG
HHHHHHHHHHHHHHHHHHHCCCEEEEEHHHCCCCCCCCCHHHHHHHHHHCCCCCEEEECC
LQRRDFIYINDLINAFKIMISKSESLISGESISIGSGHAVTIKEFVETVAKMTSYQGNLQ
CCCCCEEEHHHHHHHHHHHHHCCCHHCCCCCEECCCCCEEEHHHHHHHHHHHHCCCCCEE
FGAIPTRENELMYSCASLARIQELGWLCQYSLNSAIKDTLNRMRV
ECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GDP-4-dehydro-6-deoxy-D-mannose; GDP-4-dehydro-6-L-deoxygalactose; NADPH; Proton [C]

Specific reaction: GDP-4-dehydro-6-deoxy-D-mannose = GDP-4-dehydro-6-L-deoxygalactose GDP-4-dehydro-6-L-deoxygalactose + NADPH + Proton = guanosine diphosphate fucose + NADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2793833; 11677608; 12644504 [H]