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The map label for this gene is rluF [H]

Identifier: 170025288

GI number: 170025288

Start: 3373914

End: 3374786

Strand: Reverse

Name: rluF [H]

Synonym: YPK_3069

Alternate gene names: 170025288

Gene position: 3374786-3373914 (Counterclockwise)

Preceding gene: 170025292

Following gene: 170025287

Centisome position: 71.97

GC content: 49.71

Gene sequence:

>873_bases
ATGCTGACTAACTCATCCATTCGTCTGAACAAATACATTAGCGAGAGCGGCATCTGTTCTCGCCGCGATGGCGATCGCTA
CATTGAACAGGGCAATGTTTTTATTAACGGTAAGCGAGCCACTGTTGGCGATCAGGTATTTGCCGGAGATGTCGTGAAAG
TTAACGGTCAGATTATTGAGCCGCGTGATGAAGAAGACTTGGTGTTTATCGCGCTGAATAAGCCCGCCGGTATCATCACC
ACGATGGAAGAGGGTGAGCGGGATAACATCAGTGATTTTGTTAACCACAGCAAGCGTGTCTTTCCTATCGGGCGGTTGGA
TAAGGATTCCCAAGGGCTGATTTTTCTGACGAATCACGGCGATCTGGTTAACAAGATCCTCCGCGCCGGAAATAATCACG
AGAAAGAGTACGTGGTCACCGTTAATAAACCGGTGACCGATGAGTTTATCCAAGGCATGAGCGCGGGTGTACCGATCTTG
GGAACGGTGACCAAAAAGTGCAAAGTGCAGAAAGAAGCCCCCTTGGTGTTTCGTATCACGCTGGTGCAGGGGCTTAACCG
CCAGATTCGCCGCATGTGTAAACACTTTGGTTATGAAGTCACTAAACTTGAACGCACGCGCATCATGAACATCAGCTTGA
AAGGGCTGCCACTGGGCGAGTGGCGAGACTTGACCGACGATGAACTGCTCACGCTATTTAAGCTGATTGAAAACTCCTCT
TCCGAAGAAAAAACGGTAAAAAAAGCGCCACCTAAGCCTGTAGCCGCTAAAAGACCGGGTATTCAGGGGCAGAAAAAAGC
CGAAAAATCCGACGACAACACCGCTTCCCGCAAGCGCTTTACCCAACCTGGGCGTAAGAAGAAAGGGCGCTAA

Upstream 100 bases:

>100_bases
AAATGGCACGCTATTGGTTTGAAACTCCGGGTCGTATTACCCCGATCAAGCTTCGCAATGTACCTTGGTTAAGGTATAGT
CCGCTTTTTACGGAGGAACC

Downstream 100 bases:

>100_bases
TCCAGAGTTAAACCTGCTGGTGGCTTATTATTGCGTGTTGGTGGCTTATTATTGAAAAGTGATAAGCCCATTCACCGAGA
CTGTAAACAATATTGTGTAA

Product: 23S rRNA pseudouridine synthase F

Products: NA

Alternate protein names: rRNA pseudouridylate synthase F; rRNA-uridine isomerase F [H]

Number of amino acids: Translated: 290; Mature: 290

Protein sequence:

>290_residues
MLTNSSIRLNKYISESGICSRRDGDRYIEQGNVFINGKRATVGDQVFAGDVVKVNGQIIEPRDEEDLVFIALNKPAGIIT
TMEEGERDNISDFVNHSKRVFPIGRLDKDSQGLIFLTNHGDLVNKILRAGNNHEKEYVVTVNKPVTDEFIQGMSAGVPIL
GTVTKKCKVQKEAPLVFRITLVQGLNRQIRRMCKHFGYEVTKLERTRIMNISLKGLPLGEWRDLTDDELLTLFKLIENSS
SEEKTVKKAPPKPVAAKRPGIQGQKKAEKSDDNTASRKRFTQPGRKKKGR

Sequences:

>Translated_290_residues
MLTNSSIRLNKYISESGICSRRDGDRYIEQGNVFINGKRATVGDQVFAGDVVKVNGQIIEPRDEEDLVFIALNKPAGIIT
TMEEGERDNISDFVNHSKRVFPIGRLDKDSQGLIFLTNHGDLVNKILRAGNNHEKEYVVTVNKPVTDEFIQGMSAGVPIL
GTVTKKCKVQKEAPLVFRITLVQGLNRQIRRMCKHFGYEVTKLERTRIMNISLKGLPLGEWRDLTDDELLTLFKLIENSS
SEEKTVKKAPPKPVAAKRPGIQGQKKAEKSDDNTASRKRFTQPGRKKKGR
>Mature_290_residues
MLTNSSIRLNKYISESGICSRRDGDRYIEQGNVFINGKRATVGDQVFAGDVVKVNGQIIEPRDEEDLVFIALNKPAGIIT
TMEEGERDNISDFVNHSKRVFPIGRLDKDSQGLIFLTNHGDLVNKILRAGNNHEKEYVVTVNKPVTDEFIQGMSAGVPIL
GTVTKKCKVQKEAPLVFRITLVQGLNRQIRRMCKHFGYEVTKLERTRIMNISLKGLPLGEWRDLTDDELLTLFKLIENSS
SEEKTVKKAPPKPVAAKRPGIQGQKKAEKSDDNTASRKRFTQPGRKKKGR

Specific function: Responsible for synthesis of pseudouridine from uracil- 2604 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790453, Length=290, Percent_Identity=80, Blast_Score=449, Evalue=1e-128,
Organism=Escherichia coli, GI1787524, Length=244, Percent_Identity=31.1475409836066, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1788510, Length=237, Percent_Identity=27.4261603375527, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI87081838, Length=177, Percent_Identity=31.0734463276836, Blast_Score=83, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: NA

Molecular weight: Translated: 32604; Mature: 32604

Theoretical pI: Translated: 10.33; Mature: 10.33

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTNSSIRLNKYISESGICSRRDGDRYIEQGNVFINGKRATVGDQVFAGDVVKVNGQIIE
CCCCCCEEEHHHHCCCCCCCCCCCCHHHHCCCEEEECCCCCCCCEEEECCEEEECCEEEC
PRDEEDLVFIALNKPAGIITTMEEGERDNISDFVNHSKRVFPIGRLDKDSQGLIFLTNHG
CCCCCCEEEEEECCCCCEEEECCCCCCCCHHHHHCCCCEEEECCCCCCCCCCEEEEECCC
DLVNKILRAGNNHEKEYVVTVNKPVTDEFIQGMSAGVPILGTVTKKCKVQKEAPLVFRIT
HHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHCCCCEEEHHHHHHCCCCCCCEEEEEH
LVQGLNRQIRRMCKHFGYEVTKLERTRIMNISLKGLPLGEWRDLTDDELLTLFKLIENSS
HHHHCCHHHHHHHHHCCCEEEEECCEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCC
SEEKTVKKAPPKPVAAKRPGIQGQKKAEKSDDNTASRKRFTQPGRKKKGR
CHHHHHHCCCCCCCCCCCCCCCCCHHCCCCCCCHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MLTNSSIRLNKYISESGICSRRDGDRYIEQGNVFINGKRATVGDQVFAGDVVKVNGQIIE
CCCCCCEEEHHHHCCCCCCCCCCCCHHHHCCCEEEECCCCCCCCEEEECCEEEECCEEEC
PRDEEDLVFIALNKPAGIITTMEEGERDNISDFVNHSKRVFPIGRLDKDSQGLIFLTNHG
CCCCCCEEEEEECCCCCEEEECCCCCCCCHHHHHCCCCEEEECCCCCCCCCCEEEEECCC
DLVNKILRAGNNHEKEYVVTVNKPVTDEFIQGMSAGVPILGTVTKKCKVQKEAPLVFRIT
HHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHCCCCEEEHHHHHHCCCCCCCEEEEEH
LVQGLNRQIRRMCKHFGYEVTKLERTRIMNISLKGLPLGEWRDLTDDELLTLFKLIENSS
HHHHCCHHHHHHHHHCCCEEEEECCEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCC
SEEKTVKKAPPKPVAAKRPGIQGQKKAEKSDDNTASRKRFTQPGRKKKGR
CHHHHHHCCCCCCCCCCCCCCCCCHHCCCCCCCHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]