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The map label for this gene is rsuA [H]

Identifier: 170025018

GI number: 170025018

Start: 3083499

End: 3084206

Strand: Reverse

Name: rsuA [H]

Synonym: YPK_2793

Alternate gene names: 170025018

Gene position: 3084206-3083499 (Counterclockwise)

Preceding gene: 170025021

Following gene: 170025017

Centisome position: 65.77

GC content: 51.84

Gene sequence:

>708_bases
ATGCGATTGGACAAGTTTTTATCTCAGCAATTAGGGGTCAGCCGGGCGTTGGTAGCGCGTGAACTGCGGGCAAAGCGTGT
GACTATTGATGGCGAGGTGGTCAAAAGTGGTGCCATCAAGCTGACACCAGAACAAGACGTGCGATTTGATGGCAATCCGT
TACAGCAGATGGTTGGCCCCCGTTATTTTATGTTGAACAAACCGCAGGGCTATGTGTGTTCGACGGAAGATCCCGATCAT
CCAACGGTGTTGTATTTCCTTGATGAGCCAGTGGCTTACAAACTCCATGCCGCTGGGCGGTTGGATATCGATACTACCGG
GTTGGTCTTGATGACTGACGATGGGCAGTGGTCACATCGTGTCACTTCGCCACGTCATCATTGTGAAAAGACCTATTTGG
TGACGTTGGAACATCCGCTATCCGATGACACCGCACAGCAATTTGCTGACGGTGTCCAACTGCATAATGAAAAATCCCTG
ACCAAACCGGCTCAACTGGACGTTATCGAGCCGCAACGGGTGCGTTTGACCATCAGCGAAGGGCGTTATCATCAGGTGAA
GCGGATGTTTGCTGCTGTGGGGAACCGAGTGATTGAGTTACATCGTGAACGCATTGGTGATATCACGCTTGATGATGATT
TAGCCCCTGGTGAGTACCGGCCGCTGACGGCAGAAGAGATCGCCAGCGTGGGTGCCCCACACTTGTAA

Upstream 100 bases:

>100_bases
TTTCAACCCGTACAATTGACATCCTATTATCATCAACGGGTGTCATTATGGGGACCGTATCCATCAGTGATACGGCCATA
CAACAGGCAAAATAGATTCA

Downstream 100 bases:

>100_bases
TGAGATTGTTTGTAATGAGATTGTTATTGCCGTGCGGGCCAGCGGTTCCGCACCGTTCTACTGCGGTATAATCTGCGGTA
AGTTTTTGGAGGAGTGGTAA

Product: 16S rRNA pseudouridylate synthase A

Products: pseudouridine 5'-phosphate; H2O [C]

Alternate protein names: 16S pseudouridine 516 synthase; 16S pseudouridylate 516 synthase; rRNA pseudouridylate synthase A; rRNA-uridine isomerase A [H]

Number of amino acids: Translated: 235; Mature: 235

Protein sequence:

>235_residues
MRLDKFLSQQLGVSRALVARELRAKRVTIDGEVVKSGAIKLTPEQDVRFDGNPLQQMVGPRYFMLNKPQGYVCSTEDPDH
PTVLYFLDEPVAYKLHAAGRLDIDTTGLVLMTDDGQWSHRVTSPRHHCEKTYLVTLEHPLSDDTAQQFADGVQLHNEKSL
TKPAQLDVIEPQRVRLTISEGRYHQVKRMFAAVGNRVIELHRERIGDITLDDDLAPGEYRPLTAEEIASVGAPHL

Sequences:

>Translated_235_residues
MRLDKFLSQQLGVSRALVARELRAKRVTIDGEVVKSGAIKLTPEQDVRFDGNPLQQMVGPRYFMLNKPQGYVCSTEDPDH
PTVLYFLDEPVAYKLHAAGRLDIDTTGLVLMTDDGQWSHRVTSPRHHCEKTYLVTLEHPLSDDTAQQFADGVQLHNEKSL
TKPAQLDVIEPQRVRLTISEGRYHQVKRMFAAVGNRVIELHRERIGDITLDDDLAPGEYRPLTAEEIASVGAPHL
>Mature_235_residues
MRLDKFLSQQLGVSRALVARELRAKRVTIDGEVVKSGAIKLTPEQDVRFDGNPLQQMVGPRYFMLNKPQGYVCSTEDPDH
PTVLYFLDEPVAYKLHAAGRLDIDTTGLVLMTDDGQWSHRVTSPRHHCEKTYLVTLEHPLSDDTAQQFADGVQLHNEKSL
TKPAQLDVIEPQRVRLTISEGRYHQVKRMFAAVGNRVIELHRERIGDITLDDDLAPGEYRPLTAEEIASVGAPHL

Specific function: Responsible for synthesis of pseudouridine from uracil- 516 in 16S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788510, Length=230, Percent_Identity=82.6086956521739, Blast_Score=405, Evalue=1e-114,
Organism=Escherichia coli, GI1787524, Length=231, Percent_Identity=32.034632034632, Blast_Score=106, Evalue=1e-24,
Organism=Escherichia coli, GI1790453, Length=233, Percent_Identity=27.4678111587983, Blast_Score=89, Evalue=2e-19,
Organism=Escherichia coli, GI87081838, Length=182, Percent_Identity=32.967032967033, Blast_Score=77, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: 4.2.1.70 [C]

Molecular weight: Translated: 26485; Mature: 26485

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLDKFLSQQLGVSRALVARELRAKRVTIDGEVVKSGAIKLTPEQDVRFDGNPLQQMVGP
CCHHHHHHHHHCHHHHHHHHHHHHHEEEECHHEECCCCEEECCCCCCCCCCCHHHHHCCC
RYFMLNKPQGYVCSTEDPDHPTVLYFLDEPVAYKLHAAGRLDIDTTGLVLMTDDGQWSHR
EEEEEECCCCCEECCCCCCCCEEEEEECCCEEEEEECCCEEEECCCCEEEEECCCCCCCC
VTSPRHHCEKTYLVTLEHPLSDDTAQQFADGVQLHNEKSLTKPAQLDVIEPQRVRLTISE
CCCCHHHCCEEEEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCEEEEECCCEEEEEECC
GRYHQVKRMFAAVGNRVIELHRERIGDITLDDDLAPGEYRPLTAEEIASVGAPHL
CCHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCCCCCCCHHHHHHCCCCCC
>Mature Secondary Structure
MRLDKFLSQQLGVSRALVARELRAKRVTIDGEVVKSGAIKLTPEQDVRFDGNPLQQMVGP
CCHHHHHHHHHCHHHHHHHHHHHHHEEEECHHEECCCCEEECCCCCCCCCCCHHHHHCCC
RYFMLNKPQGYVCSTEDPDHPTVLYFLDEPVAYKLHAAGRLDIDTTGLVLMTDDGQWSHR
EEEEEECCCCCEECCCCCCCCEEEEEECCCEEEEEECCCEEEECCCCEEEEECCCCCCCC
VTSPRHHCEKTYLVTLEHPLSDDTAQQFADGVQLHNEKSLTKPAQLDVIEPQRVRLTISE
CCCCHHHCCEEEEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCEEEEECCCEEEEEECC
GRYHQVKRMFAAVGNRVIELHRERIGDITLDDDLAPGEYRPLTAEEIASVGAPHL
CCHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCCCCCCCHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose phosphate [C]

Specific reaction: uracil + D-ribose phosphate = pseudouridine 5'-phosphate + H2O [C]

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430 [H]