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The map label for this gene is bcr [H]

Identifier: 170025017

GI number: 170025017

Start: 3082190

End: 3083395

Strand: Reverse

Name: bcr [H]

Synonym: YPK_2792

Alternate gene names: 170025017

Gene position: 3083395-3082190 (Counterclockwise)

Preceding gene: 170025018

Following gene: 170025015

Centisome position: 65.75

GC content: 50.0

Gene sequence:

>1206_bases
GTGCAAAAAAACCGGACATCCCACATTGGTTTGATTTTTATCCTTGGGTTGTTGTCGATGCTGATGCCTTTGGCGATTGA
TATGTATCTGCCAAGCATGCCGATTATTGCGCAGGAGTTTGGGGTCGAAAGTGGCCGTGTGCAGATGACGCTGAGCGCAT
ATGTGCTGGGTTTTGCCATTGGGCAAATGTTCTATGGGCCAATGGCTGACAGCATCGGGCGTAAGCCGGTCATTTTTTGG
GGGACGCTGATTTTTGCGCTTGCAGGTATGGCATGTGCGTTGGTGCAGTCGGTCGAGCAACTCATTAATATGCGCTTCCT
GCATGGCTTATCCGCCGCCGCCGCAAGTGTGGTGATTAATGCCTTGATGCGCGATATGTTCACCAAAGATGAGTTCTCCC
GAATGATGTCTTTCGTGGTATTGGTGATGACGATTGCCCCCTTGCTCGCACCCATTATCGGTGGTGCACTGTTGGTGTGG
TTTAGCTGGCATGCTATTTTCTGGACCATGGCGGGGGCGGCACTGGTTGGTGCGGTGTTAGTGGCGCTGTTTATCCAAGA
AACATTGCCTCCTGAACGGCGACAGAAATTTCATCTGCGCACGACCATAGGTAATTTTGGCTCGTTATTTCGCCACAAAC
GCGTACTGAGCTATATGCTGGCCAGTGGTTTCTCTTTTGCCGGTATGTTCTCATTCCTCAGCGCCGGTCCTTTTGTTTAT
ATCGATTTAAACGGTGTTTCCCCGCAAAACTTTGGTTATTACTTTGCACTGAACATTGTCTTTTTGTTCTTGATGACCTT
TATCAACAGCCGTAATGTGCGCCGTTTCGGGGCGCTGAAAATGTTCCGTTTTGGCCTGATAGTGCAATTTGTGATGGGGA
TCTGGCTGGTAGTGGTTTGTGTCGCTGGCCTAGGATTCTGGGCCTTAGTTCTGGGCGTAGCTGCCTATGTTGGGTGTATC
GCAATGATCACGTCTAACGCGATGGCAGTGATATTAGATGATTTCCCGCATATGGCAGGGACCGCCTCTTCTTTGGCGGG
GACGTTGCGCTTTGGTATTGGTGCGGTGGTGGGGACGTTACTGTCGATGGCTTCGTCGCGCAGCGCCTGGCCAATGGTCA
GTTCAATGGCATTTTGTATTATTGTCGCGATGTTGTTGTATCTGTATGCCAGTCGCCCAGTCATAAAACCGTGTCATAAA
AAATAG

Upstream 100 bases:

>100_bases
GATTGTTTGTAATGAGATTGTTATTGCCGTGCGGGCCAGCGGTTCCGCACCGTTCTACTGCGGTATAATCTGCGGTAAGT
TTTTGGAGGAGTGGTAAAGC

Downstream 100 bases:

>100_bases
CCAGCGCCCATGCCGGATGGTATTCGATATGGATGCTATTCGACATGGATGGTATTTGACATGGGTCATGTTCGATATGA
ATTATGTTCGGCATGGGTTG

Product: bicyclomycin/multidrug efflux system

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Sulfonamide resistance protein [H]

Number of amino acids: Translated: 401; Mature: 401

Protein sequence:

>401_residues
MQKNRTSHIGLIFILGLLSMLMPLAIDMYLPSMPIIAQEFGVESGRVQMTLSAYVLGFAIGQMFYGPMADSIGRKPVIFW
GTLIFALAGMACALVQSVEQLINMRFLHGLSAAAASVVINALMRDMFTKDEFSRMMSFVVLVMTIAPLLAPIIGGALLVW
FSWHAIFWTMAGAALVGAVLVALFIQETLPPERRQKFHLRTTIGNFGSLFRHKRVLSYMLASGFSFAGMFSFLSAGPFVY
IDLNGVSPQNFGYYFALNIVFLFLMTFINSRNVRRFGALKMFRFGLIVQFVMGIWLVVVCVAGLGFWALVLGVAAYVGCI
AMITSNAMAVILDDFPHMAGTASSLAGTLRFGIGAVVGTLLSMASSRSAWPMVSSMAFCIIVAMLLYLYASRPVIKPCHK
K

Sequences:

>Translated_401_residues
MQKNRTSHIGLIFILGLLSMLMPLAIDMYLPSMPIIAQEFGVESGRVQMTLSAYVLGFAIGQMFYGPMADSIGRKPVIFW
GTLIFALAGMACALVQSVEQLINMRFLHGLSAAAASVVINALMRDMFTKDEFSRMMSFVVLVMTIAPLLAPIIGGALLVW
FSWHAIFWTMAGAALVGAVLVALFIQETLPPERRQKFHLRTTIGNFGSLFRHKRVLSYMLASGFSFAGMFSFLSAGPFVY
IDLNGVSPQNFGYYFALNIVFLFLMTFINSRNVRRFGALKMFRFGLIVQFVMGIWLVVVCVAGLGFWALVLGVAAYVGCI
AMITSNAMAVILDDFPHMAGTASSLAGTLRFGIGAVVGTLLSMASSRSAWPMVSSMAFCIIVAMLLYLYASRPVIKPCHK
K
>Mature_401_residues
MQKNRTSHIGLIFILGLLSMLMPLAIDMYLPSMPIIAQEFGVESGRVQMTLSAYVLGFAIGQMFYGPMADSIGRKPVIFW
GTLIFALAGMACALVQSVEQLINMRFLHGLSAAAASVVINALMRDMFTKDEFSRMMSFVVLVMTIAPLLAPIIGGALLVW
FSWHAIFWTMAGAALVGAVLVALFIQETLPPERRQKFHLRTTIGNFGSLFRHKRVLSYMLASGFSFAGMFSFLSAGPFVY
IDLNGVSPQNFGYYFALNIVFLFLMTFINSRNVRRFGALKMFRFGLIVQFVMGIWLVVVCVAGLGFWALVLGVAAYVGCI
AMITSNAMAVILDDFPHMAGTASSLAGTLRFGIGAVVGTLLSMASSRSAWPMVSSMAFCIIVAMLLYLYASRPVIKPCHK
K

Specific function: Involved in sulfonamide (sulfathiazole) and bicyclomycin resistance. Probable membrane translocase [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Bcr/CmlA family [H]

Homologues:

Organism=Escherichia coli, GI1788509, Length=393, Percent_Identity=69.2111959287532, Blast_Score=550, Evalue=1e-158,
Organism=Escherichia coli, GI48994889, Length=357, Percent_Identity=29.6918767507003, Blast_Score=126, Evalue=2e-30,
Organism=Escherichia coli, GI1790146, Length=384, Percent_Identity=26.5625, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI87082312, Length=340, Percent_Identity=26.1764705882353, Blast_Score=105, Evalue=4e-24,
Organism=Escherichia coli, GI1787065, Length=257, Percent_Identity=25.6809338521401, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1790794, Length=230, Percent_Identity=24.3478260869565, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1790195, Length=166, Percent_Identity=26.5060240963855, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI87081738, Length=366, Percent_Identity=26.775956284153, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1787880, Length=203, Percent_Identity=26.6009852216749, Blast_Score=63, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6319480, Length=199, Percent_Identity=25.1256281407035, Blast_Score=70, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6322071, Length=184, Percent_Identity=25.5434782608696, Blast_Score=66, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6319517, Length=187, Percent_Identity=23.5294117647059, Blast_Score=65, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6324264, Length=159, Percent_Identity=24.5283018867925, Blast_Score=63, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004812
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004734 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 43923; Mature: 43923

Theoretical pI: Translated: 10.27; Mature: 10.27

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
7.0 %Met     (Translated Protein)
8.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
7.0 %Met     (Mature Protein)
8.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure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HHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8486276; 9278503; 2694948 [H]