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The map label for this gene is ansB [H]

Identifier: 170024899

GI number: 170024899

Start: 2945545

End: 2946582

Strand: Reverse

Name: ansB [H]

Synonym: YPK_2674

Alternate gene names: 170024899

Gene position: 2946582-2945545 (Counterclockwise)

Preceding gene: 170024903

Following gene: 170024898

Centisome position: 62.83

GC content: 47.11

Gene sequence:

>1038_bases
ATGAAATATATAAAGCTAACGGTACTCGCCGGAATATTTGTAGGGATCAGTAGCCCGGTTTTTGCTCTGCCTAATATCAC
TTTATTGGCAACCGGGGGCACCATCGCAGGGGGAGGTGATTCTGCTACAAAATCCAATTATACCGCCGGTAAATTGGGGG
TAGATGCGCTGGTAGAGGCCGTCCCGGCCATAAAAGACATTGCGAATATCCAGGGCGAACAAGTGGTGAATATCGGCTCA
CAAGATATGAATGACGATGTTTGGCTCACATTGGCGAAAAAAATCAATAAAGACTGCACGAAAACAGACGGTTTTGTCAT
CACTCATGGAACGGATACTTTAGAAGAAACGGCTTATTTCCTCGATTTAACCGTCAATTGTGATAAACCCGTGGTGATAG
TCGGCGCAATGCGCCCGGCAACGGCATTAGGGGCCGACGGCCCGTTGAACCTCTACAACGCGGTGGTGGTCGCCAGTGAG
GCAGACTCGGCTAAACGGGGTGTGCTGGTGGCGATGAATGACATGGTTTTCACTGGGCGTGATGTGGTAAAAACCAACAC
CACATCAGTGCAAACATTCCAATCACCGAATACAGGGCCATTGGGGTATATCTATGATGGTAAAGTTAATTATTTGCATC
AACCAGCAGCAAGACAACCCGCGTTTGATATCAGTAAGCTAAATACGTTACCGAAAGTTGGCATTATTTATAACTATGCA
AATGCATCGGACATACCCGCAAAGGCATTGATTGCCGATGGTTATCAGGGGATTGTCAGTGCTGGCGTGGGGAACGGTAA
TCTCTATCATACGGTTTTCGATACGCTGGCAACCGCCGCAAGTCACGGGGTTGCAGTTGTGCGCTCGTCTCGTGTGCCCA
GTGGTTCAACCACAGAAGGTGCTGAAATTGATGACGCTAAATACGGCTTTGTCGCTGCGGGTGCACTTAATCCACAGAAA
GCACGGGTTTTATTACAGTTAGCATTAACGCAAACTCAAAAACCACAAGAAATTCAAAAATTATTCCACACATATTGA

Upstream 100 bases:

>100_bases
TGGTTAATAGCTCCATGTATTCTGAAAGCACAGTAGATATCACAGTTTTCATATGGATATTCCGGATAATTAATTAGCTT
ATAAATAATGGAGTAATTAT

Downstream 100 bases:

>100_bases
TACTGTGACTTTTTTGCGAAGGCTACGGTCTTTTTATTTCTAAATGAATACTACGTCCATAGGGCGTGGTATTCATTTAG
AAATAAAAATTCCCGACAGA

Product: L-asparaginase II

Products: NA

Alternate protein names: L-asparaginase II; L-ASNase II; L-asparagine amidohydrolase II; Colaspase [H]

Number of amino acids: Translated: 345; Mature: 345

Protein sequence:

>345_residues
MKYIKLTVLAGIFVGISSPVFALPNITLLATGGTIAGGGDSATKSNYTAGKLGVDALVEAVPAIKDIANIQGEQVVNIGS
QDMNDDVWLTLAKKINKDCTKTDGFVITHGTDTLEETAYFLDLTVNCDKPVVIVGAMRPATALGADGPLNLYNAVVVASE
ADSAKRGVLVAMNDMVFTGRDVVKTNTTSVQTFQSPNTGPLGYIYDGKVNYLHQPAARQPAFDISKLNTLPKVGIIYNYA
NASDIPAKALIADGYQGIVSAGVGNGNLYHTVFDTLATAASHGVAVVRSSRVPSGSTTEGAEIDDAKYGFVAAGALNPQK
ARVLLQLALTQTQKPQEIQKLFHTY

Sequences:

>Translated_345_residues
MKYIKLTVLAGIFVGISSPVFALPNITLLATGGTIAGGGDSATKSNYTAGKLGVDALVEAVPAIKDIANIQGEQVVNIGS
QDMNDDVWLTLAKKINKDCTKTDGFVITHGTDTLEETAYFLDLTVNCDKPVVIVGAMRPATALGADGPLNLYNAVVVASE
ADSAKRGVLVAMNDMVFTGRDVVKTNTTSVQTFQSPNTGPLGYIYDGKVNYLHQPAARQPAFDISKLNTLPKVGIIYNYA
NASDIPAKALIADGYQGIVSAGVGNGNLYHTVFDTLATAASHGVAVVRSSRVPSGSTTEGAEIDDAKYGFVAAGALNPQK
ARVLLQLALTQTQKPQEIQKLFHTY
>Mature_345_residues
MKYIKLTVLAGIFVGISSPVFALPNITLLATGGTIAGGGDSATKSNYTAGKLGVDALVEAVPAIKDIANIQGEQVVNIGS
QDMNDDVWLTLAKKINKDCTKTDGFVITHGTDTLEETAYFLDLTVNCDKPVVIVGAMRPATALGADGPLNLYNAVVVASE
ADSAKRGVLVAMNDMVFTGRDVVKTNTTSVQTFQSPNTGPLGYIYDGKVNYLHQPAARQPAFDISKLNTLPKVGIIYNYA
NASDIPAKALIADGYQGIVSAGVGNGNLYHTVFDTLATAASHGVAVVRSSRVPSGSTTEGAEIDDAKYGFVAAGALNPQK
ARVLLQLALTQTQKPQEIQKLFHTY

Specific function: Unknown

COG id: COG0252

COG function: function code EJ; L-asparaginase/archaeal Glu-tRNAGln amidotransferase subunit D

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the asparaginase 1 family [H]

Homologues:

Organism=Homo sapiens, GI122937331, Length=235, Percent_Identity=25.9574468085106, Blast_Score=74, Evalue=2e-13,
Organism=Escherichia coli, GI1789327, Length=348, Percent_Identity=73.2758620689655, Blast_Score=505, Evalue=1e-144,
Organism=Escherichia coli, GI1788065, Length=234, Percent_Identity=30.7692307692308, Blast_Score=85, Evalue=9e-18,
Organism=Caenorhabditis elegans, GI193207372, Length=223, Percent_Identity=30.9417040358744, Blast_Score=91, Evalue=8e-19,
Organism=Caenorhabditis elegans, GI17558280, Length=223, Percent_Identity=30.9417040358744, Blast_Score=91, Evalue=8e-19,
Organism=Caenorhabditis elegans, GI17558278, Length=223, Percent_Identity=30.9417040358744, Blast_Score=91, Evalue=9e-19,
Organism=Caenorhabditis elegans, GI193207374, Length=223, Percent_Identity=30.9417040358744, Blast_Score=91, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6323189, Length=358, Percent_Identity=39.6648044692737, Blast_Score=213, Evalue=3e-56,
Organism=Saccharomyces cerevisiae, GI6323187, Length=358, Percent_Identity=39.6648044692737, Blast_Score=213, Evalue=3e-56,
Organism=Saccharomyces cerevisiae, GI6323186, Length=358, Percent_Identity=39.6648044692737, Blast_Score=213, Evalue=3e-56,
Organism=Saccharomyces cerevisiae, GI6323184, Length=358, Percent_Identity=39.6648044692737, Blast_Score=213, Evalue=3e-56,
Organism=Saccharomyces cerevisiae, GI6320527, Length=343, Percent_Identity=37.3177842565598, Blast_Score=202, Evalue=5e-53,
Organism=Drosophila melanogaster, GI24639609, Length=224, Percent_Identity=29.4642857142857, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24645530, Length=236, Percent_Identity=27.9661016949153, Blast_Score=74, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004550
- InterPro:   IPR006034
- InterPro:   IPR020827 [H]

Pfam domain/function: PF00710 Asparaginase [H]

EC number: =3.5.1.1 [H]

Molecular weight: Translated: 36268; Mature: 36268

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: PS00144 ASN_GLN_ASE_1 ; PS00917 ASN_GLN_ASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYIKLTVLAGIFVGISSPVFALPNITLLATGGTIAGGGDSATKSNYTAGKLGVDALVEA
CCEEEEEHHHHHHHHCCCCEEECCCEEEEEECCEEECCCCCCCCCCCCCCHHHHHHHHHH
VPAIKDIANIQGEQVVNIGSQDMNDDVWLTLAKKINKDCTKTDGFVITHGTDTLEETAYF
HHHHHHHHHCCCCEEEECCCCCCCCHHEEHHHHHHCCCCCCCCCEEEECCCCCHHHCEEE
LDLTVNCDKPVVIVGAMRPATALGADGPLNLYNAVVVASEADSAKRGVLVAMNDMVFTGR
EEEEEECCCCEEEEECCCCCHHCCCCCCCCEEEEEEEEECCCCCCCCEEEEECCEEEECC
DVVKTNTTSVQTFQSPNTGPLGYIYDGKVNYLHQPAARQPAFDISKLNTLPKVGIIYNYA
CEEEECCCEEEEECCCCCCCEEEEECCEECEEECCCCCCCCCCHHHHCCCCCEEEEEEEC
NASDIPAKALIADGYQGIVSAGVGNGNLYHTVFDTLATAASHGVAVVRSSRVPSGSTTEG
CCCCCCHHHHHHCCCHHHHCCCCCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCC
AEIDDAKYGFVAAGALNPQKARVLLQLALTQTQKPQEIQKLFHTY
CCCCCCCCCEEEECCCCHHHHHHHHHHHHHCCCCHHHHHHHHHCC
>Mature Secondary Structure
MKYIKLTVLAGIFVGISSPVFALPNITLLATGGTIAGGGDSATKSNYTAGKLGVDALVEA
CCEEEEEHHHHHHHHCCCCEEECCCEEEEEECCEEECCCCCCCCCCCCCCHHHHHHHHHH
VPAIKDIANIQGEQVVNIGSQDMNDDVWLTLAKKINKDCTKTDGFVITHGTDTLEETAYF
HHHHHHHHHCCCCEEEECCCCCCCCHHEEHHHHHHCCCCCCCCCEEEECCCCCHHHCEEE
LDLTVNCDKPVVIVGAMRPATALGADGPLNLYNAVVVASEADSAKRGVLVAMNDMVFTGR
EEEEEECCCCEEEEECCCCCHHCCCCCCCCEEEEEEEEECCCCCCCCEEEEECCEEEECC
DVVKTNTTSVQTFQSPNTGPLGYIYDGKVNYLHQPAARQPAFDISKLNTLPKVGIIYNYA
CEEEECCCEEEEECCCCCCCEEEEECCEECEEECCCCCCCCCCHHHHCCCCCEEEEEEEC
NASDIPAKALIADGYQGIVSAGVGNGNLYHTVFDTLATAASHGVAVVRSSRVPSGSTTEG
CCCCCCHHHHHHCCCHHHHCCCCCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCC
AEIDDAKYGFVAAGALNPQKARVLLQLALTQTQKPQEIQKLFHTY
CCCCCCCCCEEEECCCCHHHHHHHHHHHHHCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2407723; 2144836; 9278503; 6766894; 387570; 321449; 4561256; 1906013; 1521538; 1287659; 8434007; 8706862 [H]