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The map label for this gene is yneJ [H]

Identifier: 170024795

GI number: 170024795

Start: 2822659

End: 2823528

Strand: Direct

Name: yneJ [H]

Synonym: YPK_2570

Alternate gene names: 170024795

Gene position: 2822659-2823528 (Clockwise)

Preceding gene: 170024788

Following gene: 170024796

Centisome position: 60.19

GC content: 54.02

Gene sequence:

>870_bases
ATGGATCTGACCCAGCTACGCATGTTTTGTTGTGTAGCGGAAACCGGCTCCGTTGCCCGAGCAGCGGAGCAAATGCACCG
CGTTCCTTCAAATCTCACTACGCGTCTGCGCCAGCTTGAACTGGAGCTAGGGGCTGATTTGTTTATTCGTGAAAAACAGC
GCCTGCGTTTATCACCAATGGGCCATAATTTTCTCTGTTATGCCAACCGTATTTTGGCCTTAAGCGAAGAAGCCATGAGC
ATTACCCACGCAGGCGAACCGGCGGGTAACTTCCCGTTAGGTTCAATGGAAAGCACCGCCGCCACCCGTTTACCTAATTT
GCTAGCAGCCTACCACCAGCGCTACCCCAAGGTATCGTTGTCGCTGATTACCGGAACCTCGGGTGAAATTATTGAACAAG
TCCGCGCGGGTACACTGGCAACCGCATTGGTCGATGGTCCGGTGCAGCACGATGAGTTACACGGCTGCCGCTCTTTCGAC
GAACAACTGGTGATCATTTCTTGTCTTGAACGCCCCCCCATACTGCAAGCCCGCGATGCCGTGGACGAAACGCTGTTTGC
TTTTCGCCCCAGTTGCTCTTACCGGCTGCGATTGGAAAGCTGGTTTCGACAAGTGGGCGTATTACCGGGGCATATCATGG
AAATCCAGTCCTATCATGCGATGCTGGCCTGTGTCGCCAGTGGCGCCGGGCTGGCCCTCATCCCTCTCTCAGTCCTTACG
CTGCTACCGGGCCATGAGCGGGTGCAAGTTCATCCGTTACCTCCGGATATTGCCCACACGGCAACCTGGCTTATTTGGCG
TAAAGATGCCTTCAGCCCTAATGTCCGCGCATTAAAAGAGCTGATTATCGAGAACGTTGAAATACCTTGA

Upstream 100 bases:

>100_bases
CACCCGTCAGTTAAAAGAAAGTTGCTAACATCATGCCTGAGTATTAGAATCACAAAAAATGAATAATAGTCATTAAGTTC
ATCACAAAAAGAGAACATCT

Downstream 100 bases:

>100_bases
TTGAATTGATCTGGTGGGCGGTATCTGTTCGGCCATAATCTATACATATATTTCCGCTATGATCCCGAACAACTGTTATC
AACAAATTTGATACATAAAG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 289; Mature: 289

Protein sequence:

>289_residues
MDLTQLRMFCCVAETGSVARAAEQMHRVPSNLTTRLRQLELELGADLFIREKQRLRLSPMGHNFLCYANRILALSEEAMS
ITHAGEPAGNFPLGSMESTAATRLPNLLAAYHQRYPKVSLSLITGTSGEIIEQVRAGTLATALVDGPVQHDELHGCRSFD
EQLVIISCLERPPILQARDAVDETLFAFRPSCSYRLRLESWFRQVGVLPGHIMEIQSYHAMLACVASGAGLALIPLSVLT
LLPGHERVQVHPLPPDIAHTATWLIWRKDAFSPNVRALKELIIENVEIP

Sequences:

>Translated_289_residues
MDLTQLRMFCCVAETGSVARAAEQMHRVPSNLTTRLRQLELELGADLFIREKQRLRLSPMGHNFLCYANRILALSEEAMS
ITHAGEPAGNFPLGSMESTAATRLPNLLAAYHQRYPKVSLSLITGTSGEIIEQVRAGTLATALVDGPVQHDELHGCRSFD
EQLVIISCLERPPILQARDAVDETLFAFRPSCSYRLRLESWFRQVGVLPGHIMEIQSYHAMLACVASGAGLALIPLSVLT
LLPGHERVQVHPLPPDIAHTATWLIWRKDAFSPNVRALKELIIENVEIP
>Mature_289_residues
MDLTQLRMFCCVAETGSVARAAEQMHRVPSNLTTRLRQLELELGADLFIREKQRLRLSPMGHNFLCYANRILALSEEAMS
ITHAGEPAGNFPLGSMESTAATRLPNLLAAYHQRYPKVSLSLITGTSGEIIEQVRAGTLATALVDGPVQHDELHGCRSFD
EQLVIISCLERPPILQARDAVDETLFAFRPSCSYRLRLESWFRQVGVLPGHIMEIQSYHAMLACVASGAGLALIPLSVLT
LLPGHERVQVHPLPPDIAHTATWLIWRKDAFSPNVRALKELIIENVEIP

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787806, Length=282, Percent_Identity=51.7730496453901, Blast_Score=298, Evalue=2e-82,
Organism=Escherichia coli, GI1788887, Length=293, Percent_Identity=26.2798634812287, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1790208, Length=261, Percent_Identity=26.8199233716475, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI157672245, Length=276, Percent_Identity=24.2753623188406, Blast_Score=74, Evalue=9e-15,
Organism=Escherichia coli, GI87082132, Length=245, Percent_Identity=26.530612244898, Blast_Score=68, Evalue=7e-13,
Organism=Escherichia coli, GI1787601, Length=279, Percent_Identity=24.3727598566308, Blast_Score=68, Evalue=7e-13,
Organism=Escherichia coli, GI1788297, Length=297, Percent_Identity=24.5791245791246, Blast_Score=67, Evalue=9e-13,
Organism=Escherichia coli, GI1787530, Length=293, Percent_Identity=23.5494880546075, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1790262, Length=243, Percent_Identity=24.2798353909465, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1787879, Length=283, Percent_Identity=24.3816254416961, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1788706, Length=147, Percent_Identity=31.9727891156463, Blast_Score=64, Evalue=9e-12,
Organism=Escherichia coli, GI1786401, Length=149, Percent_Identity=31.5436241610738, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32059; Mature: 32059

Theoretical pI: Translated: 6.73; Mature: 6.73

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLTQLRMFCCVAETGSVARAAEQMHRVPSNLTTRLRQLELELGADLFIREKQRLRLSPM
CCHHHHHHHHHHHCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCEEECCHHCEECCC
GHNFLCYANRILALSEEAMSITHAGEPAGNFPLGSMESTAATRLPNLLAAYHQRYPKVSL
CCCEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEE
SLITGTSGEIIEQVRAGTLATALVDGPVQHDELHGCRSFDEQLVIISCLERPPILQARDA
EEEECCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHEEEEHHCCCCCCCHHHHH
VDETLFAFRPSCSYRLRLESWFRQVGVLPGHIMEIQSYHAMLACVASGAGLALIPLSVLT
HHHHHHHCCCCCCEEEEHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
LLPGHERVQVHPLPPDIAHTATWLIWRKDAFSPNVRALKELIIENVEIP
HCCCCCEEEEECCCCCHHHHHEEEEEECCCCCCCHHHHHHHHHHCCCCC
>Mature Secondary Structure
MDLTQLRMFCCVAETGSVARAAEQMHRVPSNLTTRLRQLELELGADLFIREKQRLRLSPM
CCHHHHHHHHHHHCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCEEECCHHCEECCC
GHNFLCYANRILALSEEAMSITHAGEPAGNFPLGSMESTAATRLPNLLAAYHQRYPKVSL
CCCEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEE
SLITGTSGEIIEQVRAGTLATALVDGPVQHDELHGCRSFDEQLVIISCLERPPILQARDA
EEEECCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHEEEEHHCCCCCCCHHHHH
VDETLFAFRPSCSYRLRLESWFRQVGVLPGHIMEIQSYHAMLACVASGAGLALIPLSVLT
HHHHHHHCCCCCCEEEEHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
LLPGHERVQVHPLPPDIAHTATWLIWRKDAFSPNVRALKELIIENVEIP
HCCCCCEEEEECCCCCHHHHHEEEEEECCCCCCCHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]