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The map label for this gene is rspA [C]

Identifier: 170024733

GI number: 170024733

Start: 2743107

End: 2744384

Strand: Reverse

Name: rspA [C]

Synonym: YPK_2508

Alternate gene names: 170024733

Gene position: 2744384-2743107 (Counterclockwise)

Preceding gene: 170024734

Following gene: 170024732

Centisome position: 58.52

GC content: 50.86

Gene sequence:

>1278_bases
ATGACAATAATCACTGCATTACGCACTGAGGATGTACGTTTTCCCACCTCGTTGGAATTGGATGGTTCGGATGCAATGAA
TCCCGATCCAGACTATTCAGCAGCCTATGTGATTTTAGACACCGATAATCCGGCACTCAGTGGCCACGGTCTGACGTTCA
CCATCGGACGTGGTAATGAAATTTGCTGCGTGGCCATCCATGCATTGGAACATCTGATCGTTGGCCGTGATTTAGCGTCA
ATGACTGCCAATATGGGTAAGTTTTGGCGTGAATTTACCAGCGACAGCCAGCTACGTTGGATCGGTCCGGAGAAAGGCGC
GATTCATCTGGCCACTGGCGCGGTAATCAATGCCGTATGGGATTTATGGAGTAAGGCGGAGGGCAAGCCGCTGTGGCGTT
TAGTGGCCGATATGTCCCCTGAAGAGTTGGTGCGCTGTATTGATTTTCGCTACATCACCGACTGTATTACCCCGGAGGAG
GCATTAACGCTGTTGCAGCAGCGAGCGCATAACAAAGCCCTACGGCTGGAAAAATTGCAAGACGAAGGCTACCCTTGCTA
TACCACGTCAGCGGGCTGGTTGGGGTATCCCGATGATAAGTTACGGCGCTTATGTCAGGACGCGGTGGATGCCGGTTTTG
ACTATTTGAAACTCAAAGTGGGGCGTGATCTCGAAGACGATATTCGCCGGGTGCGCATTACCCGTGAGGTGCTTGGTCCT
GATCGTAAACTGATGATTGACGCCAATCAGATCTGGGAAACTAACGAGGCTATTCCGTGGGTGAATCAGCTCGCGTTTGC
CAATCCGTGGTTTATTGAGGAACCCACTAACCCGGATGATATCGAAGGCCATCGTCGTATTCGTCAAGGTGTTGCTCCGG
TCAAAGTGGCAACGGGCGAAATGTGCCAGAACCGCATTATGTTCAAACAATTCATCATGCGCGAAGCCATTGACGTGGTG
CAAATAGATGCCTGCCGGTTAGGCGGGGTCAACGAAGTGTTGGCGGTAATGCTGATGGCGGCAAAATACGATCTGCCAGT
GTGTCCACATGCCGGTGGTGTCGGGTTATGTGAGTATGTGCAACACCTGGCGATGATTGACTACCTGTGTATTGCCGGAA
CGGATAAAGGGCGAGTGATAGAGTATGTCGATCACTTACATGAGCATTTTGTTCACCCGTGTGTGATTAAAAACGCGGCC
TATATGCCACCCGAGCAACCGGGTTTCTCCATCGAAATGCATCAATCGTCGATAGAGAAGTACCGTTTTCGCGTTTAA

Upstream 100 bases:

>100_bases
GCAGGGCAGATAATGCGTTTGGGTATCGAAGGTCTGGGAGAACAGTGCCAGAAAACGATACAAGCGTAGTACTCATCTTT
CGCGAACGGAGAGGTTAACC

Downstream 100 bases:

>100_bases
CGTCAGTGGTAGCGCAAATCAATAGTGGTTAGTACAAATCAATAGCGATAGCACAAATCAATACCACGAGCTGAGCCAAC
AAGCAGAACGGAGGAGGAAC

Product: mandelate racemase/muconate lactonizing protein

Products: NA

Alternate protein names: Mandelate Racemase/Muconate Lactonizing; Mandelate Racemase; Racemase; Mandelate Racemase/Muconate Lactonizing-Like Protein; Mandelate Racemase/Muconate Lactonizing Family Protein; Galactonate Dehydratase; Uroporphyrinogen Decarboxylase HemE; Mandelate Racemase/Muconate Lactonizing Domain Protein; Mandelate Racemase Protein; Mandelate Racemase/Muconate Lactonizing -Like Protein; Mandelate Racemase/Muconate Lactonizing Family Protein; Dehydratase; L-Alanine-DL-Glutamate Epimerase; Isomerase Protein; Mandelate Racemase/Muconate Lactonizing Protein; DgoA Protein; Muconate Cycloisomerase; L-Fuconate Dehydratase; Dehydratase Member Of Enolase Family; Phosphopyruvate Hydratase; RTS Beta Protein; Mandelate Racemase / L-Alanine-DL-Glutamate Epimerase

Number of amino acids: Translated: 425; Mature: 424

Protein sequence:

>425_residues
MTIITALRTEDVRFPTSLELDGSDAMNPDPDYSAAYVILDTDNPALSGHGLTFTIGRGNEICCVAIHALEHLIVGRDLAS
MTANMGKFWREFTSDSQLRWIGPEKGAIHLATGAVINAVWDLWSKAEGKPLWRLVADMSPEELVRCIDFRYITDCITPEE
ALTLLQQRAHNKALRLEKLQDEGYPCYTTSAGWLGYPDDKLRRLCQDAVDAGFDYLKLKVGRDLEDDIRRVRITREVLGP
DRKLMIDANQIWETNEAIPWVNQLAFANPWFIEEPTNPDDIEGHRRIRQGVAPVKVATGEMCQNRIMFKQFIMREAIDVV
QIDACRLGGVNEVLAVMLMAAKYDLPVCPHAGGVGLCEYVQHLAMIDYLCIAGTDKGRVIEYVDHLHEHFVHPCVIKNAA
YMPPEQPGFSIEMHQSSIEKYRFRV

Sequences:

>Translated_425_residues
MTIITALRTEDVRFPTSLELDGSDAMNPDPDYSAAYVILDTDNPALSGHGLTFTIGRGNEICCVAIHALEHLIVGRDLAS
MTANMGKFWREFTSDSQLRWIGPEKGAIHLATGAVINAVWDLWSKAEGKPLWRLVADMSPEELVRCIDFRYITDCITPEE
ALTLLQQRAHNKALRLEKLQDEGYPCYTTSAGWLGYPDDKLRRLCQDAVDAGFDYLKLKVGRDLEDDIRRVRITREVLGP
DRKLMIDANQIWETNEAIPWVNQLAFANPWFIEEPTNPDDIEGHRRIRQGVAPVKVATGEMCQNRIMFKQFIMREAIDVV
QIDACRLGGVNEVLAVMLMAAKYDLPVCPHAGGVGLCEYVQHLAMIDYLCIAGTDKGRVIEYVDHLHEHFVHPCVIKNAA
YMPPEQPGFSIEMHQSSIEKYRFRV
>Mature_424_residues
TIITALRTEDVRFPTSLELDGSDAMNPDPDYSAAYVILDTDNPALSGHGLTFTIGRGNEICCVAIHALEHLIVGRDLASM
TANMGKFWREFTSDSQLRWIGPEKGAIHLATGAVINAVWDLWSKAEGKPLWRLVADMSPEELVRCIDFRYITDCITPEEA
LTLLQQRAHNKALRLEKLQDEGYPCYTTSAGWLGYPDDKLRRLCQDAVDAGFDYLKLKVGRDLEDDIRRVRITREVLGPD
RKLMIDANQIWETNEAIPWVNQLAFANPWFIEEPTNPDDIEGHRRIRQGVAPVKVATGEMCQNRIMFKQFIMREAIDVVQ
IDACRLGGVNEVLAVMLMAAKYDLPVCPHAGGVGLCEYVQHLAMIDYLCIAGTDKGRVIEYVDHLHEHFVHPCVIKNAAY
MPPEQPGFSIEMHQSSIEKYRFRV

Specific function: Probably Involved In The Degradation Of Homoserine Lactone (Hsl) Or Of A Metabolite Of Hsl That Signals Starvation. [C]

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI42544119, Length=423, Percent_Identity=53.4278959810875, Blast_Score=501, Evalue=1e-142,
Organism=Homo sapiens, GI42544117, Length=403, Percent_Identity=52.3573200992556, Blast_Score=469, Evalue=1e-132,
Organism=Homo sapiens, GI186972148, Length=331, Percent_Identity=54.0785498489426, Blast_Score=403, Evalue=1e-112,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 48030; Mature: 47898

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: PS00909 MR_MLE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIITALRTEDVRFPTSLELDGSDAMNPDPDYSAAYVILDTDNPALSGHGLTFTIGRGNE
CEEEEEECCCCCCCCCEEEECCCCCCCCCCCCCEEEEEEECCCCCCCCCCEEEEECCCCC
ICCVAIHALEHLIVGRDLASMTANMGKFWREFTSDSQLRWIGPEKGAIHLATGAVINAVW
EEEHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCEEEECCCCCEEEEEHHHHHHHHH
DLWSKAEGKPLWRLVADMSPEELVRCIDFRYITDCITPEEALTLLQQRAHNKALRLEKLQ
HHHHCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
DEGYPCYTTSAGWLGYPDDKLRRLCQDAVDAGFDYLKLKVGRDLEDDIRRVRITREVLGP
CCCCCEEECCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHCC
DRKLMIDANQIWETNEAIPWVNQLAFANPWFIEEPTNPDDIEGHRRIRQGVAPVKVATGE
CCEEEEEHHHHCCCCCCCCHHHHHHCCCCCEECCCCCCCCCHHHHHHHCCCCCEEECCHH
MCQNRIMFKQFIMREAIDVVQIDACRLGGVNEVLAVMLMAAKYDLPVCPHAGGVGLCEYV
HHHHHHHHHHHHHHHHHHHEEECCHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHH
QHLAMIDYLCIAGTDKGRVIEYVDHLHEHFVHPCVIKNAAYMPPEQPGFSIEMHQSSIEK
HHHHHHHHHHEECCCCCHHHHHHHHHHHHHCCHHHHCCCCCCCCCCCCCEEEECHHHHHH
YRFRV
HHCCC
>Mature Secondary Structure 
TIITALRTEDVRFPTSLELDGSDAMNPDPDYSAAYVILDTDNPALSGHGLTFTIGRGNE
EEEEEECCCCCCCCCEEEECCCCCCCCCCCCCEEEEEEECCCCCCCCCCEEEEECCCCC
ICCVAIHALEHLIVGRDLASMTANMGKFWREFTSDSQLRWIGPEKGAIHLATGAVINAVW
EEEHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCEEEECCCCCEEEEEHHHHHHHHH
DLWSKAEGKPLWRLVADMSPEELVRCIDFRYITDCITPEEALTLLQQRAHNKALRLEKLQ
HHHHCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
DEGYPCYTTSAGWLGYPDDKLRRLCQDAVDAGFDYLKLKVGRDLEDDIRRVRITREVLGP
CCCCCEEECCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHCC
DRKLMIDANQIWETNEAIPWVNQLAFANPWFIEEPTNPDDIEGHRRIRQGVAPVKVATGE
CCEEEEEHHHHCCCCCCCCHHHHHHCCCCCEECCCCCCCCCHHHHHHHCCCCCEEECCHH
MCQNRIMFKQFIMREAIDVVQIDACRLGGVNEVLAVMLMAAKYDLPVCPHAGGVGLCEYV
HHHHHHHHHHHHHHHHHHHEEECCHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHH
QHLAMIDYLCIAGTDKGRVIEYVDHLHEHFVHPCVIKNAAYMPPEQPGFSIEMHQSSIEK
HHHHHHHHHHEECCCCCHHHHHHHHHHHHHCCHHHHCCCCCCCCCCCCCEEEECHHHHHH
YRFRV
HHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA