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The map label for this gene is lacY [H]

Identifier: 170024717

GI number: 170024717

Start: 2724271

End: 2725578

Strand: Direct

Name: lacY [H]

Synonym: YPK_2492

Alternate gene names: 170024717

Gene position: 2724271-2725578 (Clockwise)

Preceding gene: 170024715

Following gene: 170024718

Centisome position: 58.09

GC content: 43.81

Gene sequence:

>1308_bases
ATGAGTAAAGCAAACGCAGTACAATCCTATTTAAGAAATAATAACTTCTGGTTGTTCGGGTTATTTTTCTTCTTCTACTT
TTTTATCATGGCATCGTACTACCCGTTCTTTCCGATCTGGCTGCATGATATTAATAACCTGAGCAAAACAGACACCGGTA
TTGTATTCGGTAGCATTTCACTTTTTGCCCTCGCCTTTCAGCCAATTATGGGGCCGCTCTCTGACAAGCTTGGATTACGC
AAAACCCTAATGTGGATCATTGTTGGATTACTGGTCCTGTTTGCCCCGTTCTTTATTTATGTTTTCAGCCCATTACTGAA
ATATAACATCTTCATTGGCGCTATTGTGGGTGGCTGCTATTTGGGCTTTGTATTTACTGGCGGTTCCCATGCCATTGAAG
CCTATATTGAAAAGGTCAGTCGCCACAGTAATTTTGAATACGGCCGGGTAAGAATGTTCGGCTGTATTGGCTGGGCACTG
TGCGCCACCGTGGTTGGAATACTCTACACCGTCAACAACCAATTAATTTTTTGGATGGCATCAGGCTGCGCCTTAATACT
GGCTGTCTTGCTGTTCTTCGCTCGCCCCGACCGACAATCTACGGCCTTTGTTGTCGATACTCTAGGCGCGAATAAAGCGG
TATTTAACTTAAAGAACGCGCTGGCACTCCTCCGGAAACGGGAATTGTGGTTCTTCGTTATGTATATTGTTGGCGTCGCC
TGTATTTATGATGTGTTTGATCAGCAATTTGCTAACTTTTTCACTTCATTCTTTGCCACCAAACAACAAGGAACTGAAAT
ATTCGGTTTTGTCACTACCGGTGGTGAAATTCTGAATGCAACCGTGATGTTTTTCGCTCCGGTAATTATTGCGCGTATTG
GTAGCAAAAATGCGTTATTGCTGGCAGGTACCATTATGTCAGTCCGTATTTTAGGTTCAGCATTTGCCACGACAGCGACC
CAAGTGGTGTTCCTGAAAATGTTGCATATGTTTGAGGTTCCTTTCTTACTGGTAGGCTCCTTTAAATATATTACTCAAGT
ATTTGAAGTTCGTTTTTCAGCAACGGTGTATCTGATTGGGTTCTGTTTCAGCAAACAGTTATCCATGATGTTTATGTCAG
TATTTGCTGGCCGCATGTATGACAGCATGGGATATCAAGACACGTACATGGTATTAGGCGTTATCGTCCTGTCATTTACC
CTCATTTCAGCTTTCACGCTCTCTGGGCGCAGCGCGGTAGCAAACCTGGCGTCAAGGCTAAAAGAGGATCCGTCGGTTAC
CCCTCCAGTTTCTCAGCCACAATCTTAA

Upstream 100 bases:

>100_bases
CTGAATAACGCTGGCAGCCATCGCGACAAGATCGTACAAAAAAAGGCGAGCATGTAGCAGCAATGCTCTGGCGTTTCAGC
TAATGAGAGGTTAGGGGTTT

Downstream 100 bases:

>100_bases
AAAATTCAATACAGATAACGAGGGTGTCGCCGCCATGCCATCACCTCGAGCAAATTTTCGTTTTCACAGGAGCCATTATG
AATACTACCTTTGTCAGATT

Product: galactoside permease

Products: Proton [Cytoplasm]; lactose [Cytoplasm] [C]

Alternate protein names: Lactose-proton symport [H]

Number of amino acids: Translated: 435; Mature: 434

Protein sequence:

>435_residues
MSKANAVQSYLRNNNFWLFGLFFFFYFFIMASYYPFFPIWLHDINNLSKTDTGIVFGSISLFALAFQPIMGPLSDKLGLR
KTLMWIIVGLLVLFAPFFIYVFSPLLKYNIFIGAIVGGCYLGFVFTGGSHAIEAYIEKVSRHSNFEYGRVRMFGCIGWAL
CATVVGILYTVNNQLIFWMASGCALILAVLLFFARPDRQSTAFVVDTLGANKAVFNLKNALALLRKRELWFFVMYIVGVA
CIYDVFDQQFANFFTSFFATKQQGTEIFGFVTTGGEILNATVMFFAPVIIARIGSKNALLLAGTIMSVRILGSAFATTAT
QVVFLKMLHMFEVPFLLVGSFKYITQVFEVRFSATVYLIGFCFSKQLSMMFMSVFAGRMYDSMGYQDTYMVLGVIVLSFT
LISAFTLSGRSAVANLASRLKEDPSVTPPVSQPQS

Sequences:

>Translated_435_residues
MSKANAVQSYLRNNNFWLFGLFFFFYFFIMASYYPFFPIWLHDINNLSKTDTGIVFGSISLFALAFQPIMGPLSDKLGLR
KTLMWIIVGLLVLFAPFFIYVFSPLLKYNIFIGAIVGGCYLGFVFTGGSHAIEAYIEKVSRHSNFEYGRVRMFGCIGWAL
CATVVGILYTVNNQLIFWMASGCALILAVLLFFARPDRQSTAFVVDTLGANKAVFNLKNALALLRKRELWFFVMYIVGVA
CIYDVFDQQFANFFTSFFATKQQGTEIFGFVTTGGEILNATVMFFAPVIIARIGSKNALLLAGTIMSVRILGSAFATTAT
QVVFLKMLHMFEVPFLLVGSFKYITQVFEVRFSATVYLIGFCFSKQLSMMFMSVFAGRMYDSMGYQDTYMVLGVIVLSFT
LISAFTLSGRSAVANLASRLKEDPSVTPPVSQPQS
>Mature_434_residues
SKANAVQSYLRNNNFWLFGLFFFFYFFIMASYYPFFPIWLHDINNLSKTDTGIVFGSISLFALAFQPIMGPLSDKLGLRK
TLMWIIVGLLVLFAPFFIYVFSPLLKYNIFIGAIVGGCYLGFVFTGGSHAIEAYIEKVSRHSNFEYGRVRMFGCIGWALC
ATVVGILYTVNNQLIFWMASGCALILAVLLFFARPDRQSTAFVVDTLGANKAVFNLKNALALLRKRELWFFVMYIVGVAC
IYDVFDQQFANFFTSFFATKQQGTEIFGFVTTGGEILNATVMFFAPVIIARIGSKNALLLAGTIMSVRILGSAFATTATQ
VVFLKMLHMFEVPFLLVGSFKYITQVFEVRFSATVYLIGFCFSKQLSMMFMSVFAGRMYDSMGYQDTYMVLGVIVLSFTL
ISAFTLSGRSAVANLASRLKEDPSVTPPVSQPQS

Specific function: Responsible for transport of beta-galactosides into the cell, with the concomitant import of a proton (symport system) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lacY/rafB permease family [H]

Homologues:

Organism=Escherichia coli, GI1786538, Length=413, Percent_Identity=68.7651331719128, Blast_Score=575, Evalue=1e-165,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022814
- InterPro:   IPR000576
- InterPro:   IPR018457
- InterPro:   IPR020846
- InterPro:   IPR016196 [H]

Pfam domain/function: PF01306 LacY_symp [H]

EC number: NA

Molecular weight: Translated: 48710; Mature: 48579

Theoretical pI: Translated: 9.55; Mature: 9.55

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKANAVQSYLRNNNFWLFGLFFFFYFFIMASYYPFFPIWLHDINNLSKTDTGIVFGSIS
CCHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCEEEHHHH
LFALAFQPIMGPLSDKLGLRKTLMWIIVGLLVLFAPFFIYVFSPLLKYNIFIGAIVGGCY
HHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGFVFTGGSHAIEAYIEKVSRHSNFEYGRVRMFGCIGWALCATVVGILYTVNNQLIFWMA
HHHHEECCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEC
SGCALILAVLLFFARPDRQSTAFVVDTLGANKAVFNLKNALALLRKRELWFFVMYIVGVA
CCHHHHHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
CIYDVFDQQFANFFTSFFATKQQGTEIFGFVTTGGEILNATVMFFAPVIIARIGSKNALL
HHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHCCCCCEEE
LAGTIMSVRILGSAFATTATQVVFLKMLHMFEVPFLLVGSFKYITQVFEVRFSATVYLIG
EHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCHHHHHHHHHHHHHHHHHHHH
FCFSKQLSMMFMSVFAGRMYDSMGYQDTYMVLGVIVLSFTLISAFTLSGRSAVANLASRL
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
KEDPSVTPPVSQPQS
CCCCCCCCCCCCCCC
>Mature Secondary Structure 
SKANAVQSYLRNNNFWLFGLFFFFYFFIMASYYPFFPIWLHDINNLSKTDTGIVFGSIS
CHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCEEEHHHH
LFALAFQPIMGPLSDKLGLRKTLMWIIVGLLVLFAPFFIYVFSPLLKYNIFIGAIVGGCY
HHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGFVFTGGSHAIEAYIEKVSRHSNFEYGRVRMFGCIGWALCATVVGILYTVNNQLIFWMA
HHHHEECCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEC
SGCALILAVLLFFARPDRQSTAFVVDTLGANKAVFNLKNALALLRKRELWFFVMYIVGVA
CCHHHHHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
CIYDVFDQQFANFFTSFFATKQQGTEIFGFVTTGGEILNATVMFFAPVIIARIGSKNALL
HHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHCCCCCEEE
LAGTIMSVRILGSAFATTATQVVFLKMLHMFEVPFLLVGSFKYITQVFEVRFSATVYLIG
EHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCHHHHHHHHHHHHHHHHHHHH
FCFSKQLSMMFMSVFAGRMYDSMGYQDTYMVLGVIVLSFTLISAFTLSGRSAVANLASRL
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
KEDPSVTPPVSQPQS
CCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; lactose [Periplasm] [C]

Specific reaction: Proton [Periplasm] + lactose [Periplasm] = Proton [Cytoplasm] + lactose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 6444453; 9278503; 2164211; 7578103; 1848449; 1644770; 2203471; 10485888; 12893935 [H]