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The map label for this gene is manX [H]

Identifier: 170024689

GI number: 170024689

Start: 2697931

End: 2698905

Strand: Direct

Name: manX [H]

Synonym: YPK_2464

Alternate gene names: 170024689

Gene position: 2697931-2698905 (Clockwise)

Preceding gene: 170024687

Following gene: 170024690

Centisome position: 57.53

GC content: 46.26

Gene sequence:

>975_bases
GTGAGTATAGCTATTATCATCGGCACACATGGGGCTGCGGCAGAACAACTGCTTAAAACAGCTGAAATGATTTTAGGTGA
ACAGAGTAATGTTGCTTATATCGATTTCGTCCCAGGTGAAAATGCTGAAACGTTGATTGAGAAGTACAACGATAGATTGA
CCGGGCTAGATACCAGTAAAGGTGTTCTATTCTTGGTCGATACTTGGGGTGGTAGCCCGTTTAATGCCGCCAGTCGCATT
GCTATCGATAAAGAGAACTATGAGGTCGTCACTGGCGTTAACATTCCGATGCTGGCTGAAACCTTTATGGCTCGTGATGA
TGAGCCGTCATTCGCTGAATTGGTTGCAGTAGCTGTCGAGACCGGCCGCGAGGGTGTGAAAGCCTTAAAAGCGCCTGATG
TTAAAAGTGATAAGCCCGAGACACAACCCGCAGCTCCAGTGCCAACAGCCAAGGCTCCTGCCGTTGCTTTAGGTCCGAAC
GATCATATGAAGATCGGGCTGGCACGTATTGATGACCGCTTAATCCATGGACAGGTTGCGACCCGTTGGACCAAAGAAAC
TAACGTAAACCGCATTATTGTAGTCAGTGATGAAGTTGCTGCCGACAAAATGCGTAAAACCTTGCTAACGCAAGTTGCCC
CTCCCGGTGTTACCGCACACGTGGTTGATGTAGAAAAAGCGATTCGCGTTTATAACAACCCGAAATATGCCAATGATAGG
GTCATGCTGCTCTTTACCAACCCGACTGACGTTGTTCGTCTGGTTGAGGGCGGTGTAGACATTAAATCAGTCAATATTGG
TGGTATGGCATTCCGTCAGGGGAAAACCCAGGTGAACAATGCCGTCTCCATTGATGAAAAAGATATTGAAGCGTTTAACA
AATTGAATGCTCGCGGTATTGAATTGGAAGTCCGAAAAGTGTCTTCGGATAGCCGGCTCAAAATGATGGATTTGATTAGT
AAATTAAATAAATAG

Upstream 100 bases:

>100_bases
CTATCATAATAGATTCAAGTAGCTCAACAGTATTTTTGGTGATGAGTTGTTTTTCTGTTAGTGCAATAACTCAATTTTGA
GTTAACAGAGGGGGTAGCGA

Downstream 100 bases:

>100_bases
CTCCACTAAAAACTACGTAGAAAGGTAATACCCTGTCCTATTTTGGGGCTTGGTTATTTTACTCAGCTTTATTGGTCATA
GGAGAAGTACAATGGAGATA

Product: PTS system mannose/fructose/sorbose family IIB subunit

Products: NA

Alternate protein names: EIIAB-Man; Mannose-specific phosphotransferase enzyme IIA component; EIII-Man; PTS system mannose-specific EIIA component; Mannose-specific phosphotransferase enzyme IIB component; PTS system mannose-specific EIIB component [H]

Number of amino acids: Translated: 324; Mature: 323

Protein sequence:

>324_residues
MSIAIIIGTHGAAAEQLLKTAEMILGEQSNVAYIDFVPGENAETLIEKYNDRLTGLDTSKGVLFLVDTWGGSPFNAASRI
AIDKENYEVVTGVNIPMLAETFMARDDEPSFAELVAVAVETGREGVKALKAPDVKSDKPETQPAAPVPTAKAPAVALGPN
DHMKIGLARIDDRLIHGQVATRWTKETNVNRIIVVSDEVAADKMRKTLLTQVAPPGVTAHVVDVEKAIRVYNNPKYANDR
VMLLFTNPTDVVRLVEGGVDIKSVNIGGMAFRQGKTQVNNAVSIDEKDIEAFNKLNARGIELEVRKVSSDSRLKMMDLIS
KLNK

Sequences:

>Translated_324_residues
MSIAIIIGTHGAAAEQLLKTAEMILGEQSNVAYIDFVPGENAETLIEKYNDRLTGLDTSKGVLFLVDTWGGSPFNAASRI
AIDKENYEVVTGVNIPMLAETFMARDDEPSFAELVAVAVETGREGVKALKAPDVKSDKPETQPAAPVPTAKAPAVALGPN
DHMKIGLARIDDRLIHGQVATRWTKETNVNRIIVVSDEVAADKMRKTLLTQVAPPGVTAHVVDVEKAIRVYNNPKYANDR
VMLLFTNPTDVVRLVEGGVDIKSVNIGGMAFRQGKTQVNNAVSIDEKDIEAFNKLNARGIELEVRKVSSDSRLKMMDLIS
KLNK
>Mature_323_residues
SIAIIIGTHGAAAEQLLKTAEMILGEQSNVAYIDFVPGENAETLIEKYNDRLTGLDTSKGVLFLVDTWGGSPFNAASRIA
IDKENYEVVTGVNIPMLAETFMARDDEPSFAELVAVAVETGREGVKALKAPDVKSDKPETQPAAPVPTAKAPAVALGPND
HMKIGLARIDDRLIHGQVATRWTKETNVNRIIVVSDEVAADKMRKTLLTQVAPPGVTAHVVDVEKAIRVYNNPKYANDRV
MLLFTNPTDVVRLVEGGVDIKSVNIGGMAFRQGKTQVNNAVSIDEKDIEAFNKLNARGIELEVRKVSSDSRLKMMDLISK
LNK

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG2893

COG function: function code G; Phosphotransferase system, mannose/fructose-specific component IIA

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIB type-4 domain [H]

Homologues:

Organism=Escherichia coli, GI1788120, Length=324, Percent_Identity=80.8641975308642, Blast_Score=517, Evalue=1e-148,
Organism=Escherichia coli, GI87082217, Length=157, Percent_Identity=36.3057324840764, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1789527, Length=154, Percent_Identity=34.4155844155844, Blast_Score=86, Evalue=3e-18,

Paralogues:

None

Copy number: 220 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004701
- InterPro:   IPR013789
- InterPro:   IPR004720
- InterPro:   IPR018455 [H]

Pfam domain/function: PF03610 EIIA-man; PF03830 PTSIIB_sorb [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 35188; Mature: 35057

Theoretical pI: Translated: 5.51; Mature: 5.51

Prosite motif: PS51096 PTS_EIIA_TYPE_4 ; PS51101 PTS_EIIB_TYPE_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIAIIIGTHGAAAEQLLKTAEMILGEQSNVAYIDFVPGENAETLIEKYNDRLTGLDTSK
CEEEEEEECCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHCCHHCCCCCCC
GVLFLVDTWGGSPFNAASRIAIDKENYEVVTGVNIPMLAETFMARDDEPSFAELVAVAVE
CEEEEEECCCCCCCCCCEEEEEECCCEEEEECCCCHHHHHHHHCCCCCCCHHHHHHHHHH
TGREGVKALKAPDVKSDKPETQPAAPVPTAKAPAVALGPNDHMKIGLARIDDRLIHGQVA
HCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCEEEEHHHHCCHHEECHHH
TRWTKETNVNRIIVVSDEVAADKMRKTLLTQVAPPGVTAHVVDVEKAIRVYNNPKYANDR
HEEECCCCCCEEEEEECHHHHHHHHHHHHHHCCCCCCEEEEEEHHHHHHHHCCCCCCCCE
VMLLFTNPTDVVRLVEGGVDIKSVNIGGMAFRQGKTQVNNAVSIDEKDIEAFNKLNARGI
EEEEEECCHHHHHHHHCCCEEEEEECCCEEEECCCHHCCCEEECCHHHHHHHHHCCCCCE
ELEVRKVSSDSRLKMMDLISKLNK
EEEEEECCCCCCHHHHHHHHHHCC
>Mature Secondary Structure 
SIAIIIGTHGAAAEQLLKTAEMILGEQSNVAYIDFVPGENAETLIEKYNDRLTGLDTSK
EEEEEEECCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHCCHHCCCCCCC
GVLFLVDTWGGSPFNAASRIAIDKENYEVVTGVNIPMLAETFMARDDEPSFAELVAVAVE
CEEEEEECCCCCCCCCCEEEEEECCCEEEEECCCCHHHHHHHHCCCCCCCHHHHHHHHHH
TGREGVKALKAPDVKSDKPETQPAAPVPTAKAPAVALGPNDHMKIGLARIDDRLIHGQVA
HCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCEEEEHHHHCCHHEECHHH
TRWTKETNVNRIIVVSDEVAADKMRKTLLTQVAPPGVTAHVVDVEKAIRVYNNPKYANDR
HEEECCCCCCEEEEEECHHHHHHHHHHHHHHCCCCCCEEEEEEHHHHHHHHCCCCCCCCE
VMLLFTNPTDVVRLVEGGVDIKSVNIGGMAFRQGKTQVNNAVSIDEKDIEAFNKLNARGI
EEEEEECCHHHHHHHHCCCEEEEEECCCEEEECCCHHCCCEEECCHHHHHHHHHCCCCCE
ELEVRKVSSDSRLKMMDLISKLNK
EEEEEECCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]