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The map label for this gene is purT

Identifier: 170024672

GI number: 170024672

Start: 2679254

End: 2680435

Strand: Direct

Name: purT

Synonym: YPK_2447

Alternate gene names: 170024672

Gene position: 2679254-2680435 (Clockwise)

Preceding gene: 170024666

Following gene: 170024674

Centisome position: 57.13

GC content: 53.13

Gene sequence:

>1182_bases
ATGCTAACCATTGGAACCGCTCTGCGCCCCGGCGCTACCCGTGTCATGTTGCTGGGCGCAGGCGAGCTGGGTAAAGAAGT
GGCGATTGAATGCCAAAGATTAGGACTTGAGGTGATTGCGGTTGATCGCTATGCCGATGCCCCCGCGATGCATGTTGCTC
ATCGTAGCCACGTCATTAATATGCTGGATGGTGCCGCACTCAAGCAGTTAGTCGCCCAGGAAAAACCGCATTATATCGTG
CCGGAAATCGAAGCTATCGCCACCGATATGCTGGTTGAGCTGGAGAAGATGGGCCAACATGTTGTGCCTTGTGCAGAAGC
CACCCGTCTGACCATGAACCGCGAGGGCATCCGCCGTCTGGCCGCAGAGACGTTGCAATTACCCACGTCCAGTTACCGCT
TTGCCGACACAGATAGCGCCTTCTTTCAGGCCGTGCGCGACATAGGTTACCCTTGCATTGTTAAACCGGTAATGAGTTCT
TCTGGTAAAGGCCAGAGCCTGATCCGCAGCGAGGAACACCTTCAGGCTGCTTGGGAATATGCACAACAAGGTGGCCGTGC
CGGTAGCGGGCGCGTGATTATCGAAGGTTTGGTTCATTTTGATTTTGAAATCACTTTACTGACGATCCGTGCTGTCGATG
GTATTCATTTTTGCGCCCCAATAGGCCACCGTCAGGAAGATGGCGACTATCGCGAGTCCTGGCAACCCCAAGCCATGAGT
GATATCGCCCTGCAGCGAGCAAAAGAGATCTCCGCACAAGTGGTCACCGCGTTAGGTGGGTTTGGGCTATTTGGCGTAGA
ACTGTTTGTTTGTGGTGATGACGTTATTTTCAGCGAAGTTTCTCCTCGCCCACACGATACCGGCATGGTGACCTTGATTT
CGCAGAATATGTCCGAGTTTGCCTTGCACGTGCGCGCATTTCTTGGTTTGCCGATTGGCACTATCCGCCAATATGGGGCG
GCTGCCTCGGCGGTTATTTTGCCAGAACTGACCAGCCAGAATATCACCTATCGTGGGCTGGAAACTGCCCTGATAGGCGA
TACACAGATTCGGTTATTTGGTAAACCTGAAATTGCCGGTAAGCGGCGTTTAGGGGTTGCACTGGCCGTTGCAGATAATA
TAGAAACCGCCATTGAAGTGGCAAAAAAGGCCGCGGGAAACATTGAGGTCAGTGGGGAATGA

Upstream 100 bases:

>100_bases
CCTTTAACATTACAGACGCAATCGTTTTCGTCGCTATTTTGCCGTATACTAGCGCGCGTCAAATTAACGTGTAATCTCCC
ACAGATGTAGGTATCTAACT

Downstream 100 bases:

>100_bases
TCATCCTGCTCATTTAGCCGACGCTAGCAAATAAATAAACCCGCCATTCAGCCAGCGGGTTTATTTTTATCTATCAATCG
TTCTATCGGCCAGTAACCGC

Product: phosphoribosylglycinamide formyltransferase 2

Products: 5'-Phosphoribosyl-N-Formylglycinamide; Pyrophosphate; acetylphosphate; ADP [C]

Alternate protein names: GART 2; 5'-phosphoribosylglycinamide transformylase 2; Formate-dependent GAR transformylase; GAR transformylase 2

Number of amino acids: Translated: 393; Mature: 393

Protein sequence:

>393_residues
MLTIGTALRPGATRVMLLGAGELGKEVAIECQRLGLEVIAVDRYADAPAMHVAHRSHVINMLDGAALKQLVAQEKPHYIV
PEIEAIATDMLVELEKMGQHVVPCAEATRLTMNREGIRRLAAETLQLPTSSYRFADTDSAFFQAVRDIGYPCIVKPVMSS
SGKGQSLIRSEEHLQAAWEYAQQGGRAGSGRVIIEGLVHFDFEITLLTIRAVDGIHFCAPIGHRQEDGDYRESWQPQAMS
DIALQRAKEISAQVVTALGGFGLFGVELFVCGDDVIFSEVSPRPHDTGMVTLISQNMSEFALHVRAFLGLPIGTIRQYGA
AASAVILPELTSQNITYRGLETALIGDTQIRLFGKPEIAGKRRLGVALAVADNIETAIEVAKKAAGNIEVSGE

Sequences:

>Translated_393_residues
MLTIGTALRPGATRVMLLGAGELGKEVAIECQRLGLEVIAVDRYADAPAMHVAHRSHVINMLDGAALKQLVAQEKPHYIV
PEIEAIATDMLVELEKMGQHVVPCAEATRLTMNREGIRRLAAETLQLPTSSYRFADTDSAFFQAVRDIGYPCIVKPVMSS
SGKGQSLIRSEEHLQAAWEYAQQGGRAGSGRVIIEGLVHFDFEITLLTIRAVDGIHFCAPIGHRQEDGDYRESWQPQAMS
DIALQRAKEISAQVVTALGGFGLFGVELFVCGDDVIFSEVSPRPHDTGMVTLISQNMSEFALHVRAFLGLPIGTIRQYGA
AASAVILPELTSQNITYRGLETALIGDTQIRLFGKPEIAGKRRLGVALAVADNIETAIEVAKKAAGNIEVSGE
>Mature_393_residues
MLTIGTALRPGATRVMLLGAGELGKEVAIECQRLGLEVIAVDRYADAPAMHVAHRSHVINMLDGAALKQLVAQEKPHYIV
PEIEAIATDMLVELEKMGQHVVPCAEATRLTMNREGIRRLAAETLQLPTSSYRFADTDSAFFQAVRDIGYPCIVKPVMSS
SGKGQSLIRSEEHLQAAWEYAQQGGRAGSGRVIIEGLVHFDFEITLLTIRAVDGIHFCAPIGHRQEDGDYRESWQPQAMS
DIALQRAKEISAQVVTALGGFGLFGVELFVCGDDVIFSEVSPRPHDTGMVTLISQNMSEFALHVRAFLGLPIGTIRQYGA
AASAVILPELTSQNITYRGLETALIGDTQIRLFGKPEIAGKRRLGVALAVADNIETAIEVAKKAAGNIEVSGE

Specific function: Catalyzes two reactions:the first one is the production of beta-formyl glycinamide ribonucleotide (GAR) from formate, ATP and beta GAR; the second, a side reaction, is the production of acetyl phosphate and ADP from acetate and ATP

COG id: COG0027

COG function: function code F; Formate-dependent phosphoribosylglycinamide formyltransferase (GAR transformylase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Escherichia coli, GI1788155, Length=392, Percent_Identity=78.3163265306122, Blast_Score=644, Evalue=0.0,
Organism=Escherichia coli, GI1786733, Length=245, Percent_Identity=24.4897959183673, Blast_Score=71, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6324702, Length=367, Percent_Identity=22.8882833787466, Blast_Score=96, Evalue=8e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PURT_YERPA (Q1C8V8)

Other databases:

- EMBL:   CP000308
- RefSeq:   YP_651059.1
- ProteinModelPortal:   Q1C8V8
- SMR:   Q1C8V8
- STRING:   Q1C8V8
- GeneID:   4120757
- GenomeReviews:   CP000308_GR
- KEGG:   ypa:YPA_1147
- eggNOG:   COG0027
- HOGENOM:   HBG516369
- OMA:   PDYIVPE
- ProtClustDB:   PRK09288
- BioCyc:   YPES360102:YPA_1147-MONOMER
- HAMAP:   MF_01643
- InterPro:   IPR011761
- InterPro:   IPR003135
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR005862
- InterPro:   IPR011054
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.40.50.20
- PANTHER:   PTHR23047:SF2
- TIGRFAMs:   TIGR01142

Pfam domain/function: PF02222 ATP-grasp; SSF52440 PreATP-grasp-like; SSF51246 Rudmnt_hyb_motif

EC number: 2.1.2.- [C]

Molecular weight: Translated: 42511; Mature: 42511

Theoretical pI: Translated: 5.60; Mature: 5.60

Prosite motif: PS50975 ATP_GRASP

Important sites: BINDING 82-82 BINDING 114-114 BINDING 155-155 BINDING 203-203 BINDING 267-267 BINDING 279-279 BINDING 286-286 BINDING 355-355

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTIGTALRPGATRVMLLGAGELGKEVAIECQRLGLEVIAVDRYADAPAMHVAHRSHVIN
CEEECCCCCCCCCEEEEEECCCCCHHHHHHHHHCCEEEEEEECCCCCCHHHHHHHHHHHH
MLDGAALKQLVAQEKPHYIVPEIEAIATDMLVELEKMGQHVVPCAEATRLTMNREGIRRL
HHHHHHHHHHHHCCCCCEECCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCHHHHHHH
AAETLQLPTSSYRFADTDSAFFQAVRDIGYPCIVKPVMSSSGKGQSLIRSEEHLQAAWEY
HHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCEEEHHHHCCCCCCHHHHHHHHHHHHHHHH
AQQGGRAGSGRVIIEGLVHFDFEITLLTIRAVDGIHFCAPIGHRQEDGDYRESWQPQAMS
HHCCCCCCCCCEEEEEEEEEEEEEEEEEEEEECCEEEECCCCCCCCCCCHHHCCCCHHHH
DIALQRAKEISAQVVTALGGFGLFGVELFVCGDDVIFSEVSPRPHDTGMVTLISQNMSEF
HHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCEEECCCCCCCCCCCEEEEECCCHHHH
ALHVRAFLGLPIGTIRQYGAAASAVILPELTSQNITYRGLETALIGDTQIRLFGKPEIAG
HHHHHHHHCCCHHHHHHHCCCCCEEEEECCCCCCCEECCCCEEEECCCEEEEECCCCCCC
KRRLGVALAVADNIETAIEVAKKAAGNIEVSGE
CCCCCEEEEECCCHHHHHHHHHHCCCCEEECCC
>Mature Secondary Structure
MLTIGTALRPGATRVMLLGAGELGKEVAIECQRLGLEVIAVDRYADAPAMHVAHRSHVIN
CEEECCCCCCCCCEEEEEECCCCCHHHHHHHHHCCEEEEEEECCCCCCHHHHHHHHHHHH
MLDGAALKQLVAQEKPHYIVPEIEAIATDMLVELEKMGQHVVPCAEATRLTMNREGIRRL
HHHHHHHHHHHHCCCCCEECCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCHHHHHHH
AAETLQLPTSSYRFADTDSAFFQAVRDIGYPCIVKPVMSSSGKGQSLIRSEEHLQAAWEY
HHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCEEEHHHHCCCCCCHHHHHHHHHHHHHHHH
AQQGGRAGSGRVIIEGLVHFDFEITLLTIRAVDGIHFCAPIGHRQEDGDYRESWQPQAMS
HHCCCCCCCCCEEEEEEEEEEEEEEEEEEEEECCEEEECCCCCCCCCCCHHHCCCCHHHH
DIALQRAKEISAQVVTALGGFGLFGVELFVCGDDVIFSEVSPRPHDTGMVTLISQNMSEF
HHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCEEECCCCCCCCCCCEEEEECCCHHHH
ALHVRAFLGLPIGTIRQYGAAASAVILPELTSQNITYRGLETALIGDTQIRLFGKPEIAG
HHHHHHHHCCCHHHHHHHCCCCCEEEEECCCCCCCEECCCCEEEECCCEEEEECCCCCCC
KRRLGVALAVADNIETAIEVAKKAAGNIEVSGE
CCCCCEEEEECCCHHHHHHHHHHCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Formate; 5'-Phospho-Ribosylglycinamide; acetate; ATP [C]

Specific reaction: Formate + ATP + 5'-Phospho-Ribosylglycinamide = 5'-Phosphoribosyl-N-Formylglycinamide + ADP + Pyrophosphate. acetate + ATP = acetylphosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA