Click here to switch to the map view.

The map label for this gene is dam [C]

Identifier: 170024545

GI number: 170024545

Start: 2559048

End: 2559866

Strand: Direct

Name: dam [C]

Synonym: YPK_2320

Alternate gene names: 170024545

Gene position: 2559048-2559866 (Clockwise)

Preceding gene: 170024544

Following gene: 170024546

Centisome position: 54.57

GC content: 46.15

Gene sequence:

>819_bases
ATGAAACGCATTTTTTCCCCGCTGAAATGGGCCGGCTCCAAAGGCCGTATTATGCCAACCTTGCGCCAGCATCTACCCGT
CGGAAAGCGGCTAGTCGAGCCGTTCGCCGGTTCCTGTTCCGTCATGCTGAATACTGACTATGACGAGTATCTCATTGCTG
ATATTAACGGCGATTTAATTAATTTCTATCAGCAATTACAGCGGGATTGCGAGAGCATCATTATTCTTGCAAAAGAGCTT
TTCAAATTTGATAACAGTGAGGCGAATTATTATTTGAATCGTCAACATTTCAATGAACGCGAGTTAAGTGACGAGTACCG
CGCCGCAATGTTTTTATATTTAAACCGTCACTCTCATGGTGGTATTTGCCGTTATAACCAAAAGGGTGAATTCAACGTTC
CCTACGGAAGATATAAAGCGCCCTATTTCCCCGAAGCTGAGATCCGTTATTTCGCTGAGAAATCCCAAAAGGCCACGTTT
GTATGCTGTGACTTTTTCGAAGCGCTAACCATGACTGTGCCGGGCGACGTGGTTTATTGCGATCCCCCTTACATTCCAAC
ATCCGCTACCGCAGATTTCACCAGTTACCACACAGGCGGTTTTAGTTCTAATGAGCAATTTTGGTTATCGGAAATACTCA
CGATCATAGCGGATCAAGGTTGCCACGTTATTGCATCGAACAGCGATACCCCACACGGCCGCTATCTTTACGAAAGTTTT
GATATTCACAGCATTACCGCCCCCCGCTCTGCCAGTTGCAAAGCTGGTGGCCGTAAGGCAGTAGGTGAAATTATTGCAAC
CTTGAGGGCTGCAATATGA

Upstream 100 bases:

>100_bases
CCGAACAACAGGGCGTTGCCATTGATGGTACGCCGCACCGTGCATTGGCCGATTGCAAAACAACGCTAGGTATTATCCGG
GCTATGGCAGGGGTGAAATC

Downstream 100 bases:

>100_bases
CCAAGGGTGCTTATTACAACGAGATTGATCCCTACGCCGCTCAGTGGCTGAGAAATCTTATTAAGGCCGGCCATATCAAC
CCAGGCTATGTTGACGAGCG

Product: DNA adenine methylase

Products: NA

Alternate protein names: M.Eco67Dam; ORF1-EC67 DAM [H]

Number of amino acids: Translated: 272; Mature: 272

Protein sequence:

>272_residues
MKRIFSPLKWAGSKGRIMPTLRQHLPVGKRLVEPFAGSCSVMLNTDYDEYLIADINGDLINFYQQLQRDCESIIILAKEL
FKFDNSEANYYLNRQHFNERELSDEYRAAMFLYLNRHSHGGICRYNQKGEFNVPYGRYKAPYFPEAEIRYFAEKSQKATF
VCCDFFEALTMTVPGDVVYCDPPYIPTSATADFTSYHTGGFSSNEQFWLSEILTIIADQGCHVIASNSDTPHGRYLYESF
DIHSITAPRSASCKAGGRKAVGEIIATLRAAI

Sequences:

>Translated_272_residues
MKRIFSPLKWAGSKGRIMPTLRQHLPVGKRLVEPFAGSCSVMLNTDYDEYLIADINGDLINFYQQLQRDCESIIILAKEL
FKFDNSEANYYLNRQHFNERELSDEYRAAMFLYLNRHSHGGICRYNQKGEFNVPYGRYKAPYFPEAEIRYFAEKSQKATF
VCCDFFEALTMTVPGDVVYCDPPYIPTSATADFTSYHTGGFSSNEQFWLSEILTIIADQGCHVIASNSDTPHGRYLYESF
DIHSITAPRSASCKAGGRKAVGEIIATLRAAI
>Mature_272_residues
MKRIFSPLKWAGSKGRIMPTLRQHLPVGKRLVEPFAGSCSVMLNTDYDEYLIADINGDLINFYQQLQRDCESIIILAKEL
FKFDNSEANYYLNRQHFNERELSDEYRAAMFLYLNRHSHGGICRYNQKGEFNVPYGRYKAPYFPEAEIRYFAEKSQKATF
VCCDFFEALTMTVPGDVVYCDPPYIPTSATADFTSYHTGGFSSNEQFWLSEILTIIADQGCHVIASNSDTPHGRYLYESF
DIHSITAPRSASCKAGGRKAVGEIIATLRAAI

Specific function: Methylates DNA within the sequence GATC and protects the DNA from cleavage by the restriction endonuclease MboI. May play a regulatory role in the functions of the retron [H]

COG id: COG0338

COG function: function code L; Site-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789789, Length=274, Percent_Identity=44.5255474452555, Blast_Score=239, Evalue=2e-64,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023095
- InterPro:   IPR002052
- InterPro:   IPR012327 [H]

Pfam domain/function: PF02086 MethyltransfD12 [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 30938; Mature: 30938

Theoretical pI: Translated: 6.70; Mature: 6.70

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRIFSPLKWAGSKGRIMPTLRQHLPVGKRLVEPFAGSCSVMLNTDYDEYLIADINGDLI
CCCHHCHHHHCCCCCCCCHHHHHCCCHHHHHHHCCCCCEEEEEECCCCCEEEEECCCHHH
NFYQQLQRDCESIIILAKELFKFDNSEANYYLNRQHFNERELSDEYRAAMFLYLNRHSHG
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHHHEEEEEEEECCCCC
GICRYNQKGEFNVPYGRYKAPYFPEAEIRYFAEKSQKATFVCCDFFEALTMTVPGDVVYC
CEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCEEEEHHHHHHHHEECCCCEEEE
DPPYIPTSATADFTSYHTGGFSSNEQFWLSEILTIIADQGCHVIASNSDTPHGRYLYESF
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCCCEEEEEC
DIHSITAPRSASCKAGGRKAVGEIIATLRAAI
CEEEECCCCCCCCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKRIFSPLKWAGSKGRIMPTLRQHLPVGKRLVEPFAGSCSVMLNTDYDEYLIADINGDLI
CCCHHCHHHHCCCCCCCCHHHHHCCCHHHHHHHCCCCCEEEEEECCCCCEEEEECCCHHH
NFYQQLQRDCESIIILAKELFKFDNSEANYYLNRQHFNERELSDEYRAAMFLYLNRHSHG
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHHHEEEEEEEECCCCC
GICRYNQKGEFNVPYGRYKAPYFPEAEIRYFAEKSQKATFVCCDFFEALTMTVPGDVVYC
CEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCEEEEHHHHHHHHEECCCCEEEE
DPPYIPTSATADFTSYHTGGFSSNEQFWLSEILTIIADQGCHVIASNSDTPHGRYLYESF
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCCCEEEEEC
DIHSITAPRSASCKAGGRKAVGEIIATLRAAI
CEEEECCCCCCCCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1701261 [H]