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The map label for this gene is glsA2

Identifier: 170024483

GI number: 170024483

Start: 2502530

End: 2503537

Strand: Reverse

Name: glsA2

Synonym: YPK_2254

Alternate gene names: 170024483

Gene position: 2503537-2502530 (Counterclockwise)

Preceding gene: 170024486

Following gene: 170024482

Centisome position: 53.39

GC content: 49.8

Gene sequence:

>1008_bases
ATGCCGACAGATGATAGCAATAACGGAATTATCGACTACGTATCAACAGGTCATCTACCTGACCCGGAAACAGTCGTTAA
ATTAGTGCAAGAAGCCCATAAGCGATTTAGTACGGATACTGCTGGTGTGGTTTCTAATGTTTATCCGGCATTAGAGAGAA
TTCCTGCTGATCTCTTTGGCATTTGCATGGTTGGCACTAACGGCAAAATCCACTCTGCTGGCGATGTGGATTATGAATTT
ACCATTATGAGTGTTTCCAAACCCTTCGTTTTTGCACTGGTTTGTCAGGCTATTGGGGCGAAGACCGCCCGTGAAAAACT
CGGGGTTAACAGTACCGGTATGGCGTTTAACTCGGTAACGGCCATTGAGCGGGCATCGGATGGCCGTACAAATCCAATGG
TTAACTCGGGTGCGATTGCGACGACCAGTCTGGTTCCTGGCGCGACCAGTGATGAACAATGGAAATTCATTTATGACGGT
TTGTGCCGCTTTGCTGGCCGCGAATTAACACTGAATGAAGAAGTATACCAATCGGCTTGTGAAACCAATTTCCGTAATCG
CGGTATCGCTAATGTGCTGCAAGGTTATGGGCGTCTGGGGTGTGATCCTATTATCGCCACTGATCTCTATACCCGCCAGT
GTTCACTGAATGTCAGTGCCCGAGATCTGGCTGTGATGGGCGCGACATTAGCGGACGGCGGCGTTAACCCACTGACTCGT
GAGCGAGTGGTGGACAATGATATCTGCCATTATGCATTAGCCGTCATGGTAACGGCAGGGCTGTATGAGACCTCTGGAGA
CTGGCTCTACGATATTGGCCTGCCCGGTAAAAGCGGCATTGGCGGTGGAATTGTGACAGTGTCACCTGGCAAGGGAGGGT
TGGGGACGTTCGCACCGTTACTCGACAGTGCGGGTAACAGTATCAAAGGGCAACTTGCTGCTCGGTTCTTATCACGCAGT
CTCGGGATGGATATGTTCGTTTCTGAGCCTTACACCGAAAAGAATTGA

Upstream 100 bases:

>100_bases
TCCATTTAATTTAAAACGGAATAAATATTCCTGGCGCTCTAAATAGGTTCTAGAACTAAAAAGATAGAAAATAAACAAAA
GGCGAATGGAGATAACAGAT

Downstream 100 bases:

>100_bases
TCTGTGGGATATCGATGTTGTTCTGAAAGCCAGGCGGTCTTGGCAATCAGGTTATATCGAAAATTAAGTTGTTCTAAAAA
CAAAAAATAGATCAAAAGGA

Product: glutaminase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 335; Mature: 334

Protein sequence:

>335_residues
MPTDDSNNGIIDYVSTGHLPDPETVVKLVQEAHKRFSTDTAGVVSNVYPALERIPADLFGICMVGTNGKIHSAGDVDYEF
TIMSVSKPFVFALVCQAIGAKTAREKLGVNSTGMAFNSVTAIERASDGRTNPMVNSGAIATTSLVPGATSDEQWKFIYDG
LCRFAGRELTLNEEVYQSACETNFRNRGIANVLQGYGRLGCDPIIATDLYTRQCSLNVSARDLAVMGATLADGGVNPLTR
ERVVDNDICHYALAVMVTAGLYETSGDWLYDIGLPGKSGIGGGIVTVSPGKGGLGTFAPLLDSAGNSIKGQLAARFLSRS
LGMDMFVSEPYTEKN

Sequences:

>Translated_335_residues
MPTDDSNNGIIDYVSTGHLPDPETVVKLVQEAHKRFSTDTAGVVSNVYPALERIPADLFGICMVGTNGKIHSAGDVDYEF
TIMSVSKPFVFALVCQAIGAKTAREKLGVNSTGMAFNSVTAIERASDGRTNPMVNSGAIATTSLVPGATSDEQWKFIYDG
LCRFAGRELTLNEEVYQSACETNFRNRGIANVLQGYGRLGCDPIIATDLYTRQCSLNVSARDLAVMGATLADGGVNPLTR
ERVVDNDICHYALAVMVTAGLYETSGDWLYDIGLPGKSGIGGGIVTVSPGKGGLGTFAPLLDSAGNSIKGQLAARFLSRS
LGMDMFVSEPYTEKN
>Mature_334_residues
PTDDSNNGIIDYVSTGHLPDPETVVKLVQEAHKRFSTDTAGVVSNVYPALERIPADLFGICMVGTNGKIHSAGDVDYEFT
IMSVSKPFVFALVCQAIGAKTAREKLGVNSTGMAFNSVTAIERASDGRTNPMVNSGAIATTSLVPGATSDEQWKFIYDGL
CRFAGRELTLNEEVYQSACETNFRNRGIANVLQGYGRLGCDPIIATDLYTRQCSLNVSARDLAVMGATLADGGVNPLTRE
RVVDNDICHYALAVMVTAGLYETSGDWLYDIGLPGKSGIGGGIVTVSPGKGGLGTFAPLLDSAGNSIKGQLAARFLSRSL
GMDMFVSEPYTEKN

Specific function: Unknown

COG id: COG2066

COG function: function code E; Glutaminase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutaminase family

Homologues:

Organism=Homo sapiens, GI156104878, Length=296, Percent_Identity=32.7702702702703, Blast_Score=168, Evalue=7e-42,
Organism=Homo sapiens, GI20336214, Length=296, Percent_Identity=31.7567567567568, Blast_Score=152, Evalue=5e-37,
Organism=Escherichia coli, GI1786693, Length=306, Percent_Identity=42.483660130719, Blast_Score=255, Evalue=4e-69,
Organism=Escherichia coli, GI1787804, Length=287, Percent_Identity=28.2229965156794, Blast_Score=132, Evalue=5e-32,
Organism=Caenorhabditis elegans, GI17532727, Length=297, Percent_Identity=32.6599326599327, Blast_Score=162, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI17507019, Length=319, Percent_Identity=30.0940438871473, Blast_Score=159, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI193204073, Length=311, Percent_Identity=29.2604501607717, Blast_Score=142, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI193204075, Length=313, Percent_Identity=29.073482428115, Blast_Score=142, Evalue=4e-34,
Organism=Drosophila melanogaster, GI281363241, Length=303, Percent_Identity=31.023102310231, Blast_Score=158, Evalue=4e-39,
Organism=Drosophila melanogaster, GI24653162, Length=303, Percent_Identity=31.023102310231, Blast_Score=158, Evalue=5e-39,
Organism=Drosophila melanogaster, GI24653164, Length=303, Percent_Identity=31.023102310231, Blast_Score=158, Evalue=5e-39,
Organism=Drosophila melanogaster, GI281363239, Length=303, Percent_Identity=31.023102310231, Blast_Score=158, Evalue=5e-39,
Organism=Drosophila melanogaster, GI24653158, Length=303, Percent_Identity=31.023102310231, Blast_Score=158, Evalue=5e-39,
Organism=Drosophila melanogaster, GI24653156, Length=303, Percent_Identity=31.023102310231, Blast_Score=158, Evalue=6e-39,
Organism=Drosophila melanogaster, GI116008307, Length=303, Percent_Identity=31.023102310231, Blast_Score=158, Evalue=6e-39,
Organism=Drosophila melanogaster, GI24653166, Length=303, Percent_Identity=31.023102310231, Blast_Score=157, Evalue=8e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GLSA2_YERPE (Q9ZC49)

Other databases:

- EMBL:   AL031866
- EMBL:   AL590842
- EMBL:   AE009952
- EMBL:   AE017042
- PIR:   AF0236
- PIR:   T47019
- RefSeq:   NP_669679.1
- RefSeq:   NP_993035.1
- RefSeq:   YP_002346928.1
- ProteinModelPortal:   Q9ZC49
- SMR:   Q9ZC49
- IntAct:   Q9ZC49
- GeneID:   1147319
- GeneID:   1174778
- GeneID:   2767250
- GenomeReviews:   AE009952_GR
- GenomeReviews:   AE017042_GR
- GenomeReviews:   AL590842_GR
- KEGG:   ype:YPO1939
- KEGG:   ypk:y2372
- KEGG:   ypm:YP_1682
- HOGENOM:   HBG512335
- OMA:   SGEWLFE
- ProtClustDB:   PRK12356
- BioCyc:   YPES187410:Y2372-MONOMER
- BioCyc:   YPES214092:YPO1939-MONOMER
- BRENDA:   3.5.1.2
- HAMAP:   MF_00313
- InterPro:   IPR012338
- InterPro:   IPR015868
- Gene3D:   G3DSA:3.40.710.20
- PANTHER:   PTHR12544
- TIGRFAMs:   TIGR03814

Pfam domain/function: PF04960 Glutaminase; SSF56601 PBP_transp_fold

EC number: =3.5.1.2

Molecular weight: Translated: 35591; Mature: 35459

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: PS00146 BETA_LACTAMASE_A

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTDDSNNGIIDYVSTGHLPDPETVVKLVQEAHKRFSTDTAGVVSNVYPALERIPADLFG
CCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCEE
ICMVGTNGKIHSAGDVDYEFTIMSVSKPFVFALVCQAIGAKTAREKLGVNSTGMAFNSVT
EEEECCCCCEECCCCCCEEEEEEECCCHHHHHHHHHHHCCHHHHHHCCCCCCCCCHHHHH
AIERASDGRTNPMVNSGAIATTSLVPGATSDEQWKFIYDGLCRFAGRELTLNEEVYQSAC
HHHHCCCCCCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHCCCCEEECHHHHHHHH
ETNFRNRGIANVLQGYGRLGCDPIIATDLYTRQCSLNVSARDLAVMGATLADGGVNPLTR
HCCCCCCCHHHHHHHHCCCCCCCEEECHHHEEEEECCCCHHHHHEEECHHCCCCCCCHHH
ERVVDNDICHYALAVMVTAGLYETSGDWLYDIGLPGKSGIGGGIVTVSPGKGGLGTFAPL
HHHHHHHHHHHHHHHHHHHCCEECCCCEEEEECCCCCCCCCCCEEEECCCCCCCHHHHHH
LDSAGNSIKGQLAARFLSRSLGMDMFVSEPYTEKN
HHHCCCCHHHHHHHHHHHHHCCCCEEECCCCCCCC
>Mature Secondary Structure 
PTDDSNNGIIDYVSTGHLPDPETVVKLVQEAHKRFSTDTAGVVSNVYPALERIPADLFG
CCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCEE
ICMVGTNGKIHSAGDVDYEFTIMSVSKPFVFALVCQAIGAKTAREKLGVNSTGMAFNSVT
EEEECCCCCEECCCCCCEEEEEEECCCHHHHHHHHHHHCCHHHHHHCCCCCCCCCHHHHH
AIERASDGRTNPMVNSGAIATTSLVPGATSDEQWKFIYDGLCRFAGRELTLNEEVYQSAC
HHHHCCCCCCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHCCCCEEECHHHHHHHH
ETNFRNRGIANVLQGYGRLGCDPIIATDLYTRQCSLNVSARDLAVMGATLADGGVNPLTR
HCCCCCCCHHHHHHHHCCCCCCCEEECHHHEEEEECCCCHHHHHEEECHHCCCCCCCHHH
ERVVDNDICHYALAVMVTAGLYETSGDWLYDIGLPGKSGIGGGIVTVSPGKGGLGTFAPL
HHHHHHHHHHHHHHHHHHHCCEECCCCEEEEECCCCCCCCCCCEEEECCCCCCCHHHHHH
LDSAGNSIKGQLAARFLSRSLGMDMFVSEPYTEKN
HHHCCCCHHHHHHHHHHHHHCCCCEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430