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The map label for this gene is topB [H]

Identifier: 170024322

GI number: 170024322

Start: 2316138

End: 2318066

Strand: Reverse

Name: topB [H]

Synonym: YPK_2088

Alternate gene names: 170024322

Gene position: 2318066-2316138 (Counterclockwise)

Preceding gene: 170024323

Following gene: 170024321

Centisome position: 49.43

GC content: 49.35

Gene sequence:

>1929_bases
ATGATGCGACTGTTTATTGCCGAAAAACCCAGTTTAGCGCGAGCGATTGCAGATATTTTGCCAAAACCGCATCGTCGAGG
TGATGGATTCATCGCCTGTGGTAATGATCAAATGGTGACCTGGTGTGTTGGTCATTTACTCGAACAAGCCCAGCCGGATG
CTTATGACAGCCGTTATGCACGTTGGTCATTAGCGGACTTACCGATCATTCCTGAAAAATGGCAGCTACAACCTCGTCCT
TCCGTCAGCAAACAACTCAATGTGATTAAGCAACTGTTACAGCAAGCCGATGAAGTGGTCCATGCTGGCGACCCGGATAG
AGAAGGGCAACTGTTAGTTGATGAGGTGCTGGATTATTTGGGATTGGCCGCAGAGAAACGGCAAAATGTTCGTCGTTGCT
TGATTAACGATTTAAACCCACAGGCGGTTGAGCGTGCAGTGGAGCGCTTGCGTTATAACCGCGAGTTTATCCCGCTGTGT
GTTTCTGCGTTGGCTCGAGCCAGAGCCGATTGGCTCTATGGCATCAATATGACTCGCGCCTATACCATTCTTGGGCGCAA
TGCGGGCTATGACGGTGTTTTATCCGTCGGGCGGGTACAAACGCCCGTATTGGGGCTGGTTGTTCGCCGTGATGAAGAGA
TTGAAAGCTTCATTCCCAAAGATTTTTTTGAAGTTAAAGCCCACATAGTGACGCCTGCAGACGAACGTTTTATTGCTCTC
TGGCAGCCGAGTGACTCCTGCGAATCTTATCAGGACGAAGAAGGGCGCTTGTTGCATCGGCCGCTTGCAGAACATGTTGT
CAATCGCATAACCGGCCAGCCCGCGCTGGTGACATCCTATAATGATAAACGGGAATCGGAAACGGCACCGTTGCCATTCT
CCTTGTCTACATTACAAATCGAAGCTGCAAAACGTTTCGGCCTCAGTGCTCAGCAAGTATTGGATATTTGCCAACGGTTG
TATGAAACGCACAAATTGATTACGTATCCCCGTTCGGATTGCCGATATCTACCTGAAGAGCATTTTGCCGGGCGTCATTC
GGTGCTTAATGCCATTAGTCTTCATCAGTCAGACCTTTTGCCGCTTGAGGTCATGGATAGTGATCGGCGCAACCGCTGCT
GGGATGATAAAAAAGTCGATGCGCACCACGCTATTATCCCAACCGCACGTGCCAGCAAAGTGAATTTGACGACGGATGAA
GGCAAAGTATACCGGTTGGTGGCACAGCAATATCTGATGCAATTCTGTCCAGATGCACAGTTCCGTAAGTGTGTGATTGA
ATTGGATATCGCTGGCGGGAAATTTATTGCCAAGGCACGCTTTTTGGCCGAGGCGGGATGGCGGACCTTACTGGGCAGTA
AAGAGCGTGATGAAGAGAACGAAGGAGCGCCATTGCCGGTGGTAGCCAAAGGGGCTGAATTATTATGCGAACGCGGTGAG
GTTGTTGAACGACAAACTCAGCCACCCCGGCCTTTCACCGACGCCAGTTTATTATCGGCGATGACAGGAATTGCGCGTTT
CGTACAGGATAAAGAACTGAAAAAAGTTTTGCGAGCCACCGATGGCTTGGGTACGGAAGCCACTCGAGCAGGTATTATCG
AACTGCTCTTCAAGCGCACTTTTCTCTTTAAGAAAGGGCGTTATATACATGCCAGCCCTGCGGGACGCGCGTTGATTCAT
GCATTACCGGATATTGCGGCACGGCCAGATATGACAGCACATTGGGAATCAACGTTGACACAAATCAGTGAAAAAAACTG
CCGCTATCAAGATTTTATGCAACCACTGGTGATGACATTGCAGGATTTAATTAGTCAGGCGAAACAAAATCGTTCTCCAC
AGGTTTTCCGTGGGTTACCCGCCGGCCCTTCAGGGGCACCCAAGAAACGTAAAAAAATATCGTCTAAGGCCAAGGAGAGT
AAATCATGA

Upstream 100 bases:

>100_bases
CGGGCGCTGATTGAGATTGTAGTGTGATGGATTGTAGTGTGATGGATTGTAGTGTGATGGATTGTAGCGTGATGTTTTCT
TTGACGTATTTATGCGGTGA

Downstream 100 bases:

>100_bases
AAACACGGTTGCTATTGAGTTGCATTGGCTTTGCCACACTTATTTCTGGTGCGGCATTAGCGAATCGTAGCAATGTTGAT
GTGATTGTTCCTTTGCCACC

Product: DNA topoisomerase III

Products: NA

Alternate protein names: DNA topoisomerase III [H]

Number of amino acids: Translated: 642; Mature: 642

Protein sequence:

>642_residues
MMRLFIAEKPSLARAIADILPKPHRRGDGFIACGNDQMVTWCVGHLLEQAQPDAYDSRYARWSLADLPIIPEKWQLQPRP
SVSKQLNVIKQLLQQADEVVHAGDPDREGQLLVDEVLDYLGLAAEKRQNVRRCLINDLNPQAVERAVERLRYNREFIPLC
VSALARARADWLYGINMTRAYTILGRNAGYDGVLSVGRVQTPVLGLVVRRDEEIESFIPKDFFEVKAHIVTPADERFIAL
WQPSDSCESYQDEEGRLLHRPLAEHVVNRITGQPALVTSYNDKRESETAPLPFSLSTLQIEAAKRFGLSAQQVLDICQRL
YETHKLITYPRSDCRYLPEEHFAGRHSVLNAISLHQSDLLPLEVMDSDRRNRCWDDKKVDAHHAIIPTARASKVNLTTDE
GKVYRLVAQQYLMQFCPDAQFRKCVIELDIAGGKFIAKARFLAEAGWRTLLGSKERDEENEGAPLPVVAKGAELLCERGE
VVERQTQPPRPFTDASLLSAMTGIARFVQDKELKKVLRATDGLGTEATRAGIIELLFKRTFLFKKGRYIHASPAGRALIH
ALPDIAARPDMTAHWESTLTQISEKNCRYQDFMQPLVMTLQDLISQAKQNRSPQVFRGLPAGPSGAPKKRKKISSKAKES
KS

Sequences:

>Translated_642_residues
MMRLFIAEKPSLARAIADILPKPHRRGDGFIACGNDQMVTWCVGHLLEQAQPDAYDSRYARWSLADLPIIPEKWQLQPRP
SVSKQLNVIKQLLQQADEVVHAGDPDREGQLLVDEVLDYLGLAAEKRQNVRRCLINDLNPQAVERAVERLRYNREFIPLC
VSALARARADWLYGINMTRAYTILGRNAGYDGVLSVGRVQTPVLGLVVRRDEEIESFIPKDFFEVKAHIVTPADERFIAL
WQPSDSCESYQDEEGRLLHRPLAEHVVNRITGQPALVTSYNDKRESETAPLPFSLSTLQIEAAKRFGLSAQQVLDICQRL
YETHKLITYPRSDCRYLPEEHFAGRHSVLNAISLHQSDLLPLEVMDSDRRNRCWDDKKVDAHHAIIPTARASKVNLTTDE
GKVYRLVAQQYLMQFCPDAQFRKCVIELDIAGGKFIAKARFLAEAGWRTLLGSKERDEENEGAPLPVVAKGAELLCERGE
VVERQTQPPRPFTDASLLSAMTGIARFVQDKELKKVLRATDGLGTEATRAGIIELLFKRTFLFKKGRYIHASPAGRALIH
ALPDIAARPDMTAHWESTLTQISEKNCRYQDFMQPLVMTLQDLISQAKQNRSPQVFRGLPAGPSGAPKKRKKISSKAKES
KS
>Mature_642_residues
MMRLFIAEKPSLARAIADILPKPHRRGDGFIACGNDQMVTWCVGHLLEQAQPDAYDSRYARWSLADLPIIPEKWQLQPRP
SVSKQLNVIKQLLQQADEVVHAGDPDREGQLLVDEVLDYLGLAAEKRQNVRRCLINDLNPQAVERAVERLRYNREFIPLC
VSALARARADWLYGINMTRAYTILGRNAGYDGVLSVGRVQTPVLGLVVRRDEEIESFIPKDFFEVKAHIVTPADERFIAL
WQPSDSCESYQDEEGRLLHRPLAEHVVNRITGQPALVTSYNDKRESETAPLPFSLSTLQIEAAKRFGLSAQQVLDICQRL
YETHKLITYPRSDCRYLPEEHFAGRHSVLNAISLHQSDLLPLEVMDSDRRNRCWDDKKVDAHHAIIPTARASKVNLTTDE
GKVYRLVAQQYLMQFCPDAQFRKCVIELDIAGGKFIAKARFLAEAGWRTLLGSKERDEENEGAPLPVVAKGAELLCERGE
VVERQTQPPRPFTDASLLSAMTGIARFVQDKELKKVLRATDGLGTEATRAGIIELLFKRTFLFKKGRYIHASPAGRALIH
ALPDIAARPDMTAHWESTLTQISEKNCRYQDFMQPLVMTLQDLISQAKQNRSPQVFRGLPAGPSGAPKKRKKISSKAKES
KS

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another. TOP3 is a potent decatenase [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI4507635, Length=624, Percent_Identity=22.9166666666667, Blast_Score=112, Evalue=9e-25,
Organism=Escherichia coli, GI1788061, Length=641, Percent_Identity=82.9953198127925, Blast_Score=1117, Evalue=0.0,
Organism=Escherichia coli, GI1787529, Length=646, Percent_Identity=23.0650154798762, Blast_Score=127, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI32563869, Length=618, Percent_Identity=23.462783171521, Blast_Score=110, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI17555378, Length=628, Percent_Identity=24.5222929936306, Blast_Score=103, Evalue=4e-22,
Organism=Saccharomyces cerevisiae, GI6323263, Length=675, Percent_Identity=24.2962962962963, Blast_Score=101, Evalue=4e-22,
Organism=Drosophila melanogaster, GI24640096, Length=627, Percent_Identity=22.6475279106858, Blast_Score=115, Evalue=9e-26,
Organism=Drosophila melanogaster, GI24585251, Length=701, Percent_Identity=23.6804564907275, Blast_Score=98, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR005738
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 72453; Mature: 72453

Theoretical pI: Translated: 8.62; Mature: 8.62

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMRLFIAEKPSLARAIADILPKPHRRGDGFIACGNDQMVTWCVGHLLEQAQPDAYDSRYA
CEEEEECCCCHHHHHHHHHCCCCCCCCCCEEEECCCCHHHHHHHHHHHHCCCCCHHCHHC
RWSLADLPIIPEKWQLQPRPSVSKQLNVIKQLLQQADEVVHAGDPDREGQLLVDEVLDYL
EEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHH
GLAAEKRQNVRRCLINDLNPQAVERAVERLRYNREFIPLCVSALARARADWLYGINMTRA
CCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCHHHE
YTILGRNAGYDGVLSVGRVQTPVLGLVVRRDEEIESFIPKDFFEVKAHIVTPADERFIAL
EEEEECCCCCCHHHHCCCHHCCHHHHHCCCCHHHHHHCCHHHHHHHHHEECCCCCCEEEE
WQPSDSCESYQDEEGRLLHRPLAEHVVNRITGQPALVTSYNDKRESETAPLPFSLSTLQI
ECCCCCHHCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCEEHHHEEE
EAAKRFGLSAQQVLDICQRLYETHKLITYPRSDCRYLPEEHFAGRHSVLNAISLHQSDLL
HHHHHHCCCHHHHHHHHHHHHHHHHEEECCCHHHCCCCHHHCCCHHHHHHHHHHHHCCCC
PLEVMDSDRRNRCWDDKKVDAHHAIIPTARASKVNLTTDEGKVYRLVAQQYLMQFCPDAQ
CHHCCCCCCCCCCCCCCCCCCCCEECCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCHH
FRKCVIELDIAGGKFIAKARFLAEAGWRTLLGSKERDEENEGAPLPVVAKGAELLCERGE
HEEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEECCHHHHHHCCH
VVERQTQPPRPFTDASLLSAMTGIARFVQDKELKKVLRATDGLGTEATRAGIIELLFKRT
HHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
FLFKKGRYIHASPAGRALIHALPDIAARPDMTAHWESTLTQISEKNCRYQDFMQPLVMTL
HHHHCCCEEECCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHH
QDLISQAKQNRSPQVFRGLPAGPSGAPKKRKKISSKAKESKS
HHHHHHHHHCCCCHHHCCCCCCCCCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure
MMRLFIAEKPSLARAIADILPKPHRRGDGFIACGNDQMVTWCVGHLLEQAQPDAYDSRYA
CEEEEECCCCHHHHHHHHHCCCCCCCCCCEEEECCCCHHHHHHHHHHHHCCCCCHHCHHC
RWSLADLPIIPEKWQLQPRPSVSKQLNVIKQLLQQADEVVHAGDPDREGQLLVDEVLDYL
EEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHH
GLAAEKRQNVRRCLINDLNPQAVERAVERLRYNREFIPLCVSALARARADWLYGINMTRA
CCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCHHHE
YTILGRNAGYDGVLSVGRVQTPVLGLVVRRDEEIESFIPKDFFEVKAHIVTPADERFIAL
EEEEECCCCCCHHHHCCCHHCCHHHHHCCCCHHHHHHCCHHHHHHHHHEECCCCCCEEEE
WQPSDSCESYQDEEGRLLHRPLAEHVVNRITGQPALVTSYNDKRESETAPLPFSLSTLQI
ECCCCCHHCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCEEHHHEEE
EAAKRFGLSAQQVLDICQRLYETHKLITYPRSDCRYLPEEHFAGRHSVLNAISLHQSDLL
HHHHHHCCCHHHHHHHHHHHHHHHHEEECCCHHHCCCCHHHCCCHHHHHHHHHHHHCCCC
PLEVMDSDRRNRCWDDKKVDAHHAIIPTARASKVNLTTDEGKVYRLVAQQYLMQFCPDAQ
CHHCCCCCCCCCCCCCCCCCCCCEECCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCHH
FRKCVIELDIAGGKFIAKARFLAEAGWRTLLGSKERDEENEGAPLPVVAKGAELLCERGE
HEEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEECCHHHHHHCCH
VVERQTQPPRPFTDASLLSAMTGIARFVQDKELKKVLRATDGLGTEATRAGIIELLFKRT
HHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
FLFKKGRYIHASPAGRALIHALPDIAARPDMTAHWESTLTQISEKNCRYQDFMQPLVMTL
HHHHCCCEEECCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHH
QDLISQAKQNRSPQVFRGLPAGPSGAPKKRKKISSKAKESKS
HHHHHHHHHCCCCHHHCCCCCCCCCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2553698; 9097039; 9278503; 10574789 [H]