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The map label for this gene is oppA [H]

Identifier: 170024305

GI number: 170024305

Start: 2296993

End: 2298630

Strand: Reverse

Name: oppA [H]

Synonym: YPK_2070

Alternate gene names: 170024305

Gene position: 2298630-2296993 (Counterclockwise)

Preceding gene: 170024306

Following gene: 170024304

Centisome position: 49.02

GC content: 42.61

Gene sequence:

>1638_bases
ATGACCAACATCACAAAGAAGAATCTCATTACAGCTGCTGTAGCCGTTGCAATGAGCATGATGGCTGCGGGAAATACGTT
TGCTGCCAATGTTCCCACAGGCGTACAACTGGCTGAGAAGCAAGTGTTAGTTCGTAATAATGGCTCCGAGCCTCAATCGT
TGGATCCACATAAAATTGAAGGGGTACCTGAATCCAATATTTCACGTGATCTACTTGAAGGGCTGGTGATTAATGACCCT
AATGGCAATATTGTACCGGGAGCCGCAGAGAGTTGGGATAATAAAGACTTTAAAGTCTGGACGTTTAATATTCGTAAAGA
TGCCAAATGGTCAAATGGTGACCCTGTAACGGCACAGGATTTTGTTTACAGTTGGCAACGTCTGGCTGATCCTAAAACAG
TCTCTCCTTATGCAAGCTATTTGCAATACGCTCATCTCACTAATATCGATGACATTATTACCGGTAAAGCCGCGCCTGAC
ACGTTGGGTGTAAAAGCGTTGGATGATCATACGCTGGAAGTGACATTGTCTGAGCCTGTTCCTTATTTGGATAAGCTGTT
AGCTCACCCGTCAATGTCACCGGTGAATAAAACCGTGGTCGAGAAATTTGGTGAAAAATGGACCCAACCACAAAATTTTG
TGGGGAATGGTGCTTATAAACTAAAAGATTGGATTGTTAACGAACGTATCGTATTAGAACGCAGTCCTACGTATTGGGAT
AATGCGAAAACAGTCATCAATCAGGTGACTTATTTGCCAATTTCTTCTGAAGTGACGGATGTTAACCGCTATCGCAGCGG
CGAAATTGATATGACGTATAACAACATGCCAATTGAATTATTCCAAAAACTGAAAAAAGAAATTCCAGATCAAGTCCATG
TTGATCCCTATCTGTGTACTTATTACTACGAAATTAACAATCAAAAAGCCCCTTTTACGGATGCTCGTGTACGTGAAGCG
CTGAAATTGGGTATGGACCGCGATATCATCGTCAATAAAGTTAAAAATCAGGGAGATTTACCCGCTTATGGTTTCACCCC
ACCCTATACCAGTGGCGCGGAGCTGACGCCGCCAGAGTGGTTTAGCTGGACACAAGAAAAACGTAATGAAGTAGCGAAAA
AACTATTGGCTGAAGCCGGGTACACTAAAGACAATCCACTGAAGTTTAGCTTGTTATATAACACCTCCGATTTACATAAA
AAATTAGCCATTGCTGCCGCCTCTATTTGGAAGAAAAATCTGGGTGTAGACGTTAAACTGGAAAACCAAGAGTGGAAAAC
GTTCTTGGATACCCGTCACCAGGGGACTTATGATGTTGCCCGTGCCGCTTGGTGTGCCGATTACAATGAGCCATCCTCCT
TCCTAAATATGATGTTATCTAACAGTAGCAACAACACCACCCATTATAAAAGTTCGGTATTTGACAAGCTGATAGAAGAC
ACGTTGAAAGTGAAATCGGAAAAAGAGCGTGCTGACTTGTATCAACAAGCGGAAATCCAGTTGGATAAAGATTCTGCCAT
TGTGCCCGTGTTCTATTACGTGAGTGCGCGTTTAGTGAAACCTTATGTTGGTGGCTACACGGGTAAGGATCCACTGGACA
ATATGCACGTTAAAGATCTTTATATTATCAAGCAGTAA

Upstream 100 bases:

>100_bases
ACCGGGTTCGTTCCCCAGGTAACGCAAATTTAGAATACCGTTTAAAGGTGAGTTTGCCTGTTCACTGGGCAATACATAAT
AAAATGGAGTACATATAAAC

Downstream 100 bases:

>100_bases
TGTATGAAGGGCAGGCAACTGAGTTGGTGGCTGCCCTTATTGCACTAATGATATAAAGTCGTCGTTAAAGGCTAAAGGTA
TAGGCAATGTTAAAGTTTAT

Product: extracellular solute-binding protein

Products: ADP; phosphate; oligopeptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 545; Mature: 544

Protein sequence:

>545_residues
MTNITKKNLITAAVAVAMSMMAAGNTFAANVPTGVQLAEKQVLVRNNGSEPQSLDPHKIEGVPESNISRDLLEGLVINDP
NGNIVPGAAESWDNKDFKVWTFNIRKDAKWSNGDPVTAQDFVYSWQRLADPKTVSPYASYLQYAHLTNIDDIITGKAAPD
TLGVKALDDHTLEVTLSEPVPYLDKLLAHPSMSPVNKTVVEKFGEKWTQPQNFVGNGAYKLKDWIVNERIVLERSPTYWD
NAKTVINQVTYLPISSEVTDVNRYRSGEIDMTYNNMPIELFQKLKKEIPDQVHVDPYLCTYYYEINNQKAPFTDARVREA
LKLGMDRDIIVNKVKNQGDLPAYGFTPPYTSGAELTPPEWFSWTQEKRNEVAKKLLAEAGYTKDNPLKFSLLYNTSDLHK
KLAIAAASIWKKNLGVDVKLENQEWKTFLDTRHQGTYDVARAAWCADYNEPSSFLNMMLSNSSNNTTHYKSSVFDKLIED
TLKVKSEKERADLYQQAEIQLDKDSAIVPVFYYVSARLVKPYVGGYTGKDPLDNMHVKDLYIIKQ

Sequences:

>Translated_545_residues
MTNITKKNLITAAVAVAMSMMAAGNTFAANVPTGVQLAEKQVLVRNNGSEPQSLDPHKIEGVPESNISRDLLEGLVINDP
NGNIVPGAAESWDNKDFKVWTFNIRKDAKWSNGDPVTAQDFVYSWQRLADPKTVSPYASYLQYAHLTNIDDIITGKAAPD
TLGVKALDDHTLEVTLSEPVPYLDKLLAHPSMSPVNKTVVEKFGEKWTQPQNFVGNGAYKLKDWIVNERIVLERSPTYWD
NAKTVINQVTYLPISSEVTDVNRYRSGEIDMTYNNMPIELFQKLKKEIPDQVHVDPYLCTYYYEINNQKAPFTDARVREA
LKLGMDRDIIVNKVKNQGDLPAYGFTPPYTSGAELTPPEWFSWTQEKRNEVAKKLLAEAGYTKDNPLKFSLLYNTSDLHK
KLAIAAASIWKKNLGVDVKLENQEWKTFLDTRHQGTYDVARAAWCADYNEPSSFLNMMLSNSSNNTTHYKSSVFDKLIED
TLKVKSEKERADLYQQAEIQLDKDSAIVPVFYYVSARLVKPYVGGYTGKDPLDNMHVKDLYIIKQ
>Mature_544_residues
TNITKKNLITAAVAVAMSMMAAGNTFAANVPTGVQLAEKQVLVRNNGSEPQSLDPHKIEGVPESNISRDLLEGLVINDPN
GNIVPGAAESWDNKDFKVWTFNIRKDAKWSNGDPVTAQDFVYSWQRLADPKTVSPYASYLQYAHLTNIDDIITGKAAPDT
LGVKALDDHTLEVTLSEPVPYLDKLLAHPSMSPVNKTVVEKFGEKWTQPQNFVGNGAYKLKDWIVNERIVLERSPTYWDN
AKTVINQVTYLPISSEVTDVNRYRSGEIDMTYNNMPIELFQKLKKEIPDQVHVDPYLCTYYYEINNQKAPFTDARVREAL
KLGMDRDIIVNKVKNQGDLPAYGFTPPYTSGAELTPPEWFSWTQEKRNEVAKKLLAEAGYTKDNPLKFSLLYNTSDLHKK
LAIAAASIWKKNLGVDVKLENQEWKTFLDTRHQGTYDVARAAWCADYNEPSSFLNMMLSNSSNNTTHYKSSVFDKLIEDT
LKVKSEKERADLYQQAEIQLDKDSAIVPVFYYVSARLVKPYVGGYTGKDPLDNMHVKDLYIIKQ

Specific function: This protein is a component of the oligopeptide permease, a binding protein-dependent transport system, it binds peptides up to five amino acids long with high affinity [H]

COG id: COG4166

COG function: function code E; ABC-type oligopeptide transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787495, Length=545, Percent_Identity=76.697247706422, Blast_Score=877, Evalue=0.0,
Organism=Escherichia coli, GI87081878, Length=534, Percent_Identity=47.3782771535581, Blast_Score=510, Evalue=1e-146,
Organism=Escherichia coli, GI1789397, Length=526, Percent_Identity=42.9657794676806, Blast_Score=444, Evalue=1e-126,
Organism=Escherichia coli, GI1787762, Length=499, Percent_Identity=27.0541082164329, Blast_Score=142, Evalue=6e-35,
Organism=Escherichia coli, GI1789966, Length=555, Percent_Identity=23.0630630630631, Blast_Score=111, Evalue=9e-26,
Organism=Escherichia coli, GI1789887, Length=499, Percent_Identity=25.8517034068136, Blast_Score=110, Evalue=3e-25,
Organism=Escherichia coli, GI1787052, Length=527, Percent_Identity=22.2011385199241, Blast_Score=102, Evalue=7e-23,
Organism=Escherichia coli, GI1787551, Length=454, Percent_Identity=24.0088105726872, Blast_Score=79, Evalue=6e-16,

Paralogues:

None

Copy number: 760 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2360 Molecules/Cell In: Stationary-Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 61619; Mature: 61488

Theoretical pI: Translated: 6.09; Mature: 6.09

Prosite motif: PS01040 SBP_BACTERIAL_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNITKKNLITAAVAVAMSMMAAGNTFAANVPTGVQLAEKQVLVRNNGSEPQSLDPHKIE
CCCCCHHHHHHHHHHHHHHHHHCCCEEECCCCCCCHHHHCEEEEECCCCCCCCCCCHHCC
GVPESNISRDLLEGLVINDPNGNIVPGAAESWDNKDFKVWTFNIRKDAKWSNGDPVTAQD
CCCCCCCHHHHHCCEEEECCCCCCCCCCCCCCCCCCEEEEEEEEECCCCCCCCCCCCHHH
FVYSWQRLADPKTVSPYASYLQYAHLTNIDDIITGKAAPDTLGVKALDDHTLEVTLSEPV
HHHHHHHHCCCCCCCHHHHHHHHHHHCCCHHHHCCCCCCCCCCCEEECCCEEEEEECCCC
PYLDKLLAHPSMSPVNKTVVEKFGEKWTQPQNFVGNGAYKLKDWIVNERIVLERSPTYWD
HHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCHHCCCCCEEHHHHHCCCEEEEECCCCCCC
NAKTVINQVTYLPISSEVTDVNRYRSGEIDMTYNNMPIELFQKLKKEIPDQVHVDPYLCT
CHHHHHHHHEEECCCCCHHHHHHHCCCCEEEEECCCCHHHHHHHHHHCCCCCCCCCEEEE
YYYEINNQKAPFTDARVREALKLGMDRDIIVNKVKNQGDLPAYGFTPPYTSGAELTPPEW
EEEEECCCCCCCHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHH
FSWTQEKRNEVAKKLLAEAGYTKDNPLKFSLLYNTSDLHKKLAIAAASIWKKNLGVDVKL
HHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHCCCEEEEE
ENQEWKTFLDTRHQGTYDVARAAWCADYNEPSSFLNMMLSNSSNNTTHYKSSVFDKLIED
CCCHHHHHHHHCCCCCHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
TLKVKSEKERADLYQQAEIQLDKDSAIVPVFYYVSARLVKPYVGGYTGKDPLDNMHVKDL
HHHHCCHHHHHHHHHHHHEEECCCCCEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEE
YIIKQ
EEEEC
>Mature Secondary Structure 
TNITKKNLITAAVAVAMSMMAAGNTFAANVPTGVQLAEKQVLVRNNGSEPQSLDPHKIE
CCCCHHHHHHHHHHHHHHHHHCCCEEECCCCCCCHHHHCEEEEECCCCCCCCCCCHHCC
GVPESNISRDLLEGLVINDPNGNIVPGAAESWDNKDFKVWTFNIRKDAKWSNGDPVTAQD
CCCCCCCHHHHHCCEEEECCCCCCCCCCCCCCCCCCEEEEEEEEECCCCCCCCCCCCHHH
FVYSWQRLADPKTVSPYASYLQYAHLTNIDDIITGKAAPDTLGVKALDDHTLEVTLSEPV
HHHHHHHHCCCCCCCHHHHHHHHHHHCCCHHHHCCCCCCCCCCCEEECCCEEEEEECCCC
PYLDKLLAHPSMSPVNKTVVEKFGEKWTQPQNFVGNGAYKLKDWIVNERIVLERSPTYWD
HHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCHHCCCCCEEHHHHHCCCEEEEECCCCCCC
NAKTVINQVTYLPISSEVTDVNRYRSGEIDMTYNNMPIELFQKLKKEIPDQVHVDPYLCT
CHHHHHHHHEEECCCCCHHHHHHHCCCCEEEEECCCCHHHHHHHHHHCCCCCCCCCEEEE
YYYEINNQKAPFTDARVREALKLGMDRDIIVNKVKNQGDLPAYGFTPPYTSGAELTPPEW
EEEEECCCCCCCHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHH
FSWTQEKRNEVAKKLLAEAGYTKDNPLKFSLLYNTSDLHKKLAIAAASIWKKNLGVDVKL
HHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHCCCEEEEE
ENQEWKTFLDTRHQGTYDVARAAWCADYNEPSSFLNMMLSNSSNNTTHYKSSVFDKLIED
CCCHHHHHHHHCCCCCHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
TLKVKSEKERADLYQQAEIQLDKDSAIVPVFYYVSARLVKPYVGGYTGKDPLDNMHVKDL
HHHHCCHHHHHHHHHHHHEEECCCCCEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEE
YIIKQ
EEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; oligopeptides [Periplasm]; H2O [C]

Specific reaction: ATP + oligopeptides [Periplasm] + H2O = ADP + phosphate + oligopeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2821267; 3525163; 11677609; 8202710; 8747465; 10422831; 10438628 [H]