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The map label for this gene is rluB [H]

Identifier: 170024274

GI number: 170024274

Start: 2269988

End: 2271022

Strand: Reverse

Name: rluB [H]

Synonym: YPK_2039

Alternate gene names: 170024274

Gene position: 2271022-2269988 (Counterclockwise)

Preceding gene: 170024275

Following gene: 170024271

Centisome position: 48.43

GC content: 51.01

Gene sequence:

>1035_bases
ATGAGCGAGAAGTCAGAAAAGCAAAAGTTACCAACCCCAAATCCACACACCCCTGCTAAGCCACAGGGCGCTAAAATTGT
GGGTGAAAAATTACAGAAAATCCTGGCGCGCGCTGGCCATGGTTCTCGTCGTGAAATTGAAGCCATTATCCAGCAAGGCC
GCGTCAGTGTTGACGGTAAAGTGTCAAAGCTAGGTGATCGTGTAGAAGTGACCCAGTCCACTAAAATTCGTCTGGATGGT
CATCTTCTTTCAATTAAAGAATCTGAAGAAAATGTGTGCCGCGTTCTGGCGTATTACAAGCCAGAAGGTGAGCTTTGTAC
GCGTAATGATCCGGAAGGTCGTCCAACGGTATTTGACCGTTTACCTAAACTGCGTGGTTCACGTTGGGTTGCGGTTGGTC
GTTTGGATGTGAACACCTCTGGTTTATTACTGTTCACTACCGATGGCGAATTGGCTAACCGCCTGATGCACCCAAGCCGT
GAAGTTGAGCGTGAATATGCGGTGCGGGTATTTGGTCAAATCGATGATGATAAAATCAAACAGTTGAGCCGTGGTGTACA
ACTGGAAGATGGCCCTGCGGCATTTCGTACTATCAGCTACCAGGGTGGTGAAGGGATAAACCAGTGGTATAACGTTACGT
TGACCGAAGGGCGTAATCGCGAAGTTCGCCGTCTATGGGAAGCCGTAGGCGTGCAGGTTAGCCGCCTGATTCGTGTGCGT
TATGGCGACATTAATTTGCCGAAAGGCCTGCCGCGGGGTGGCTGGACTGAACTGGATCTTAAAGCCACTAACTACCTGCG
TGAATTGGTCGAACTGGATGTTGAAACCGTCAGTAAATTACCCGTTGAGAAAGATCGTCGCCGGGTTAAAGCCAACCAAA
TCCGTCGTGCAGTTAAGCGCCATACCGAAGTCTCGGGCCGCCAGGTTGCTGGCCGTCAGGGTTCAGCCCGTAAAGGCTCT
ACGCGTCAAAACGTGGGTAATGCGGTACCCGCAGCCACGGCAAGTCGCCGTAGTGGCCCGAAAAAACGCGGTTAA

Upstream 100 bases:

>100_bases
AATGTATTACAATCAGTGGGTTATATTTTAATTTTATCCTTAACGTATTGATTACAATATATAACACGACGCCTGTGAAG
GCGACATTAGAGGTTGCTCA

Downstream 100 bases:

>100_bases
TCTCAGGCGGATTAATGCGTCGTTAACCGCGTCTGTCATTAGACATCAATAGCGACGTGAATAATGCTTATTGCCGCAGT
TTTTTGGACTGCGGCAATAT

Product: 23S rRNA pseudouridylate synthase B

Products: NA

Alternate protein names: rRNA pseudouridylate synthase B; rRNA-uridine isomerase B [H]

Number of amino acids: Translated: 344; Mature: 343

Protein sequence:

>344_residues
MSEKSEKQKLPTPNPHTPAKPQGAKIVGEKLQKILARAGHGSRREIEAIIQQGRVSVDGKVSKLGDRVEVTQSTKIRLDG
HLLSIKESEENVCRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGSRWVAVGRLDVNTSGLLLFTTDGELANRLMHPSR
EVEREYAVRVFGQIDDDKIKQLSRGVQLEDGPAAFRTISYQGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVR
YGDINLPKGLPRGGWTELDLKATNYLRELVELDVETVSKLPVEKDRRRVKANQIRRAVKRHTEVSGRQVAGRQGSARKGS
TRQNVGNAVPAATASRRSGPKKRG

Sequences:

>Translated_344_residues
MSEKSEKQKLPTPNPHTPAKPQGAKIVGEKLQKILARAGHGSRREIEAIIQQGRVSVDGKVSKLGDRVEVTQSTKIRLDG
HLLSIKESEENVCRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGSRWVAVGRLDVNTSGLLLFTTDGELANRLMHPSR
EVEREYAVRVFGQIDDDKIKQLSRGVQLEDGPAAFRTISYQGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVR
YGDINLPKGLPRGGWTELDLKATNYLRELVELDVETVSKLPVEKDRRRVKANQIRRAVKRHTEVSGRQVAGRQGSARKGS
TRQNVGNAVPAATASRRSGPKKRG
>Mature_343_residues
SEKSEKQKLPTPNPHTPAKPQGAKIVGEKLQKILARAGHGSRREIEAIIQQGRVSVDGKVSKLGDRVEVTQSTKIRLDGH
LLSIKESEENVCRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGSRWVAVGRLDVNTSGLLLFTTDGELANRLMHPSRE
VEREYAVRVFGQIDDDKIKQLSRGVQLEDGPAAFRTISYQGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRY
GDINLPKGLPRGGWTELDLKATNYLRELVELDVETVSKLPVEKDRRRVKANQIRRAVKRHTEVSGRQVAGRQGSARKGST
RQNVGNAVPAATASRRSGPKKRG

Specific function: Responsible for synthesis of pseudouridine from uracil- 2605 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787524, Length=291, Percent_Identity=83.1615120274914, Blast_Score=498, Evalue=1e-142,
Organism=Escherichia coli, GI1790453, Length=253, Percent_Identity=30.4347826086957, Blast_Score=104, Evalue=7e-24,
Organism=Escherichia coli, GI1788510, Length=234, Percent_Identity=30.3418803418803, Blast_Score=99, Evalue=5e-22,
Organism=Escherichia coli, GI87081838, Length=193, Percent_Identity=33.160621761658, Blast_Score=84, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: NA

Molecular weight: Translated: 38516; Mature: 38385

Theoretical pI: Translated: 10.88; Mature: 10.88

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEKSEKQKLPTPNPHTPAKPQGAKIVGEKLQKILARAGHGSRREIEAIIQQGRVSVDGK
CCCCCCHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCH
VSKLGDRVEVTQSTKIRLDGHLLSIKESEENVCRVLAYYKPEGELCTRNDPEGRPTVFDR
HHHCCCEEEECCCCEEEECCEEEEEECCHHHHEEEHEEECCCCCCCCCCCCCCCCHHHHH
LPKLRGSRWVAVGRLDVNTSGLLLFTTDGELANRLMHPSREVEREYAVRVFGQIDDDKIK
HHHHCCCEEEEEEEEEECCCCEEEEECCHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHH
QLSRGVQLEDGPAAFRTISYQGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVR
HHHCCCCCCCCCCEEEEEEECCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHE
YGDINLPKGLPRGGWTELDLKATNYLRELVELDVETVSKLPVEKDRRRVKANQIRRAVKR
ECCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
HTEVSGRQVAGRQGSARKGSTRQNVGNAVPAATASRRSGPKKRG
HHHCCCHHHCCCCCCCCCCCCHHHCCCCCCCCHHHHCCCCCCCC
>Mature Secondary Structure 
SEKSEKQKLPTPNPHTPAKPQGAKIVGEKLQKILARAGHGSRREIEAIIQQGRVSVDGK
CCCCCHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCH
VSKLGDRVEVTQSTKIRLDGHLLSIKESEENVCRVLAYYKPEGELCTRNDPEGRPTVFDR
HHHCCCEEEECCCCEEEECCEEEEEECCHHHHEEEHEEECCCCCCCCCCCCCCCCHHHHH
LPKLRGSRWVAVGRLDVNTSGLLLFTTDGELANRLMHPSREVEREYAVRVFGQIDDDKIK
HHHHCCCEEEEEEEEEECCCCEEEEECCHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHH
QLSRGVQLEDGPAAFRTISYQGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVR
HHHCCCCCCCCCCEEEEEEECCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHE
YGDINLPKGLPRGGWTELDLKATNYLRELVELDVETVSKLPVEKDRRRVKANQIRRAVKR
ECCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
HTEVSGRQVAGRQGSARKGSTRQNVGNAVPAATASRRSGPKKRG
HHHCCCHHHCCCCCCCCCCCCHHHCCCCCCCCHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430 [H]