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The map label for this gene is clpB [H]

Identifier: 170023715

GI number: 170023715

Start: 1629626

End: 1632283

Strand: Direct

Name: clpB [H]

Synonym: YPK_1474

Alternate gene names: 170023715

Gene position: 1629626-1632283 (Clockwise)

Preceding gene: 170023706

Following gene: 170023716

Centisome position: 34.75

GC content: 48.12

Gene sequence:

>2658_bases
GTGGCTATTACAAGGAAACATCTCTTCGGCAAGCTGGACGCCACTCTTTTCAAAGGTATTGAAAGCGCAACAACGATTTG
TAAATTACGCGGCAATCCTTACGTAGAGTTAGTTCACTGGTTGAATCAGCTTTGGCATCAGGAAGATAACGACCTTAAGC
AGATTATTCGGTATTTTGCGGTGGATGTGGATGCCTTTGAGCGTGAGCTGGCTCAGGCACTTGCGAAGTTACCTGTCGGG
GCAACTAGCATTTCAGATTTTTCGTATCATATTGAGTTGGCCACGGAACGGGCTTGGATCTATGCCAGCCTGGAGTGTTT
AGATACCCGTATCCGGAGTGGACACCTGCTGCTTGCGCTATTAACAACAATGGAACTGCGGAGAGCTTTCTTTGCGATTG
CGCCAAGCATGGAAAAAATTCCATTAGAGCATTTAAGCAAAGATTTGAACTTCATTACGCAGGCATCACCAGAAGCGAAT
GAAGCAGCCAGTGATGGCAGCCCGTTGTACGATGGAGCTTTACCTGGCGAAGCCAGTAATGCCATCGGTGCGGCAAAAAG
TGGTGGCACACTGGCTCAATACACCACAGACCTCACGGCATTAGCACGTGAAGGCAAGATAGATCCGGTATTGGGACGTA
ATCACGAAATCAACACAATGGTTGATATTCTCTTGCGCCGTCGGCAGAACAACCCATTACTGACCGGTGACGCAGGGGTG
GGTAAAACCGCCATTGTTGAAGGGCTAGCATTGGCCATTGCTGCAGGCTCAATGCCGCCAGCATTAAGCCAGGTAAGCCT
GTTGTCATTAGATATTGTGGCGCTCTCAGCGGGTGCCAGCATGAAAGGTGAATTTGAAGCGCGCCTGAAGAGTGTCTTGG
ATGAAGCAATCGCGGCAGAAAAGCCCGTTATTTTGTTTATCGATGAAGTCCACACACTTATTGGCGCGGGTGGCAATGCC
GGGACCGGCGATGCGGCGAACTTATTGAAACCTGCATTGGCGCGTGGCCAATTACGTACCATTGGTGCGACAACGTGGAG
CGAGTTCAAGCGTCACATTGAGAAGGATCCGGCACTCACCCGTCGTTTCCAAGTACTTCAAGTTGATGAGCCCGATGAGA
ACACGGCGATCTCCATGTTACGGGGCCTGATACCTGCGCTAGAAAAGCACCATGGCGTGTGGATTATGGACGAAGCCTTA
CAGGCGGCAGTACGGTTGTCTCATCGTTATATCCCTGCGCGTCAGTTGCCGGATAAAGCGATAAGTCTGCTTGATACGGC
TTGTGCCCGGATCGCTGTTGCACAGCATTCACAACCAATAGAGTTGCAGCACTTAACCTTCCAGAGTGAGACCGCGCAAA
CTGAGCTGGTTTCCTTAGAGAAAGCGCGGCACTTTGGTAAAGCGCAGGATGCGCGTACTGAGCAGTTGAAAACATCCATT
GCTGAACATGGCGAGGCTGCAGATAAACTTGATCAGCGTTGGCAGGCAGAACGCGAATTGGTATCAGCGATTACGACCAT
AAGAACCGCACTTTATGACTTGGTTTCTCAGCCAGAGCCTGATGAGGAAAAACGTCGGGCTTATCAAGCGCAATTAGTCC
AGTTGGAAGCGCAGCTTTCTCAGGTTCGCACTTCACTGCCGTTAGTGCAGACAGAAGTCAACGCTGAAGTGATCGCCAGT
ATCGTTGCAGATTGGACCGGCATCCCGGTTGGGCAAATGCTCAAAGACGATATTCGGGCGGTAATGGAGTTGCCACAGCG
CCTTGAAGCACGTGTTATTGGTCAGCCCCATGCATTGATGCAACTGGGTGAGAATATTATGACCGCACGCGCCGGTCTGT
CAGACCCAAGGAAACCATTGGGGGTCTTTATGTTAGTGGGGCCTTCAGGTGTGGGTAAAACAGAGACGGCCTTAGCGATT
GCCGAGAGCATGTATGGTGGTGAACAGAATATGATCACTATCAATATGAGCGAATATCAGGAATCGCATACCGTTTCCTC
TTTAAAAGGTTCTCCACCGGGATATGTTGGATACGGTGAAGGCGGTGTATTAACGGAAGCCGTGCGCCGTAAGCCATATA
GTGTTGTGTTATTAGATGAAATCGAAAAAGCGCACTCTGATGTACATGAATTATTCTTCCAAGTGTTTGATAAAGGCCAA
ATGGAAGATGGTGAAGGGCGCTTTATCGATTTCAAAAACACTATTTTGCTGTTAACCAGTAATGTGGGCAGCGAGTTACT
GAGTAACTTATTAGCTGATCCAGATACTGCACCGGATCAGGATGGAATATTAACTGCGTTACAACCTGAATTGCTGAAAG
TTTTCCCAGCAGCATTTTTAGGGCGAGTCACGGTTATTCCTTATCTTCCGTTACAGCAAGATGCATTGCAGCACATTGTA
CGGTTACATCTTGACCGGATTGGCGCTCGTTTATACTCGCAGCATCAATTGACACTGAAATACAGTGATGAAGTGGTTGA
CGATGTTGTTAGCCGCTGTGCGGTTTCAGAAACCGGTGCACGTATGCTGATCCGTTACATTGAACAAAATATTACGCCAA
AAATAGGTAAATATATTTTAGGTGATAGTGATGTGAAGCCTGAGCAAATTATTTTCGTTCATAAAAATGAAAATGGATTC
GTGATCGCTTTACAATGA

Upstream 100 bases:

>100_bases
TTAGTCTTAGCCGCAGAAATAGTTCATGGTGCATCAAGCATTATTTCATGAACAATTTAAACTACACATGAACGCCGTTA
AAAAGACCAGGATAAAAAAC

Downstream 100 bases:

>100_bases
TGGAAACTAAAGATATCACGTTTAAACGGGTGTCGATCCTATAGACTTTTATCTCAGTAATACTTATTGCAGATGGACAT
GCTAAAGGATGAAAAGGAAT

Product: type VI secretion ATPase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 885; Mature: 884

Protein sequence:

>885_residues
MAITRKHLFGKLDATLFKGIESATTICKLRGNPYVELVHWLNQLWHQEDNDLKQIIRYFAVDVDAFERELAQALAKLPVG
ATSISDFSYHIELATERAWIYASLECLDTRIRSGHLLLALLTTMELRRAFFAIAPSMEKIPLEHLSKDLNFITQASPEAN
EAASDGSPLYDGALPGEASNAIGAAKSGGTLAQYTTDLTALAREGKIDPVLGRNHEINTMVDILLRRRQNNPLLTGDAGV
GKTAIVEGLALAIAAGSMPPALSQVSLLSLDIVALSAGASMKGEFEARLKSVLDEAIAAEKPVILFIDEVHTLIGAGGNA
GTGDAANLLKPALARGQLRTIGATTWSEFKRHIEKDPALTRRFQVLQVDEPDENTAISMLRGLIPALEKHHGVWIMDEAL
QAAVRLSHRYIPARQLPDKAISLLDTACARIAVAQHSQPIELQHLTFQSETAQTELVSLEKARHFGKAQDARTEQLKTSI
AEHGEAADKLDQRWQAERELVSAITTIRTALYDLVSQPEPDEEKRRAYQAQLVQLEAQLSQVRTSLPLVQTEVNAEVIAS
IVADWTGIPVGQMLKDDIRAVMELPQRLEARVIGQPHALMQLGENIMTARAGLSDPRKPLGVFMLVGPSGVGKTETALAI
AESMYGGEQNMITINMSEYQESHTVSSLKGSPPGYVGYGEGGVLTEAVRRKPYSVVLLDEIEKAHSDVHELFFQVFDKGQ
MEDGEGRFIDFKNTILLLTSNVGSELLSNLLADPDTAPDQDGILTALQPELLKVFPAAFLGRVTVIPYLPLQQDALQHIV
RLHLDRIGARLYSQHQLTLKYSDEVVDDVVSRCAVSETGARMLIRYIEQNITPKIGKYILGDSDVKPEQIIFVHKNENGF
VIALQ

Sequences:

>Translated_885_residues
MAITRKHLFGKLDATLFKGIESATTICKLRGNPYVELVHWLNQLWHQEDNDLKQIIRYFAVDVDAFERELAQALAKLPVG
ATSISDFSYHIELATERAWIYASLECLDTRIRSGHLLLALLTTMELRRAFFAIAPSMEKIPLEHLSKDLNFITQASPEAN
EAASDGSPLYDGALPGEASNAIGAAKSGGTLAQYTTDLTALAREGKIDPVLGRNHEINTMVDILLRRRQNNPLLTGDAGV
GKTAIVEGLALAIAAGSMPPALSQVSLLSLDIVALSAGASMKGEFEARLKSVLDEAIAAEKPVILFIDEVHTLIGAGGNA
GTGDAANLLKPALARGQLRTIGATTWSEFKRHIEKDPALTRRFQVLQVDEPDENTAISMLRGLIPALEKHHGVWIMDEAL
QAAVRLSHRYIPARQLPDKAISLLDTACARIAVAQHSQPIELQHLTFQSETAQTELVSLEKARHFGKAQDARTEQLKTSI
AEHGEAADKLDQRWQAERELVSAITTIRTALYDLVSQPEPDEEKRRAYQAQLVQLEAQLSQVRTSLPLVQTEVNAEVIAS
IVADWTGIPVGQMLKDDIRAVMELPQRLEARVIGQPHALMQLGENIMTARAGLSDPRKPLGVFMLVGPSGVGKTETALAI
AESMYGGEQNMITINMSEYQESHTVSSLKGSPPGYVGYGEGGVLTEAVRRKPYSVVLLDEIEKAHSDVHELFFQVFDKGQ
MEDGEGRFIDFKNTILLLTSNVGSELLSNLLADPDTAPDQDGILTALQPELLKVFPAAFLGRVTVIPYLPLQQDALQHIV
RLHLDRIGARLYSQHQLTLKYSDEVVDDVVSRCAVSETGARMLIRYIEQNITPKIGKYILGDSDVKPEQIIFVHKNENGF
VIALQ
>Mature_884_residues
AITRKHLFGKLDATLFKGIESATTICKLRGNPYVELVHWLNQLWHQEDNDLKQIIRYFAVDVDAFERELAQALAKLPVGA
TSISDFSYHIELATERAWIYASLECLDTRIRSGHLLLALLTTMELRRAFFAIAPSMEKIPLEHLSKDLNFITQASPEANE
AASDGSPLYDGALPGEASNAIGAAKSGGTLAQYTTDLTALAREGKIDPVLGRNHEINTMVDILLRRRQNNPLLTGDAGVG
KTAIVEGLALAIAAGSMPPALSQVSLLSLDIVALSAGASMKGEFEARLKSVLDEAIAAEKPVILFIDEVHTLIGAGGNAG
TGDAANLLKPALARGQLRTIGATTWSEFKRHIEKDPALTRRFQVLQVDEPDENTAISMLRGLIPALEKHHGVWIMDEALQ
AAVRLSHRYIPARQLPDKAISLLDTACARIAVAQHSQPIELQHLTFQSETAQTELVSLEKARHFGKAQDARTEQLKTSIA
EHGEAADKLDQRWQAERELVSAITTIRTALYDLVSQPEPDEEKRRAYQAQLVQLEAQLSQVRTSLPLVQTEVNAEVIASI
VADWTGIPVGQMLKDDIRAVMELPQRLEARVIGQPHALMQLGENIMTARAGLSDPRKPLGVFMLVGPSGVGKTETALAIA
ESMYGGEQNMITINMSEYQESHTVSSLKGSPPGYVGYGEGGVLTEAVRRKPYSVVLLDEIEKAHSDVHELFFQVFDKGQM
EDGEGRFIDFKNTILLLTSNVGSELLSNLLADPDTAPDQDGILTALQPELLKVFPAAFLGRVTVIPYLPLQQDALQHIVR
LHLDRIGARLYSQHQLTLKYSDEVVDDVVSRCAVSETGARMLIRYIEQNITPKIGKYILGDSDVKPEQIIFVHKNENGFV
IALQ

Specific function: Part of a stress-induced multi-chaperone system, it is involved in the recovery of the cell from heat-induced damage, in cooperation with DnaK, DnaJ and GrpE. Acts before DnaK, in the processing of protein aggregates. Protein binding stimulates the ATPase

COG id: COG0542

COG function: function code O; ATPases with chaperone activity, ATP-binding subunit

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the clpA/clpB family [H]

Homologues:

Organism=Homo sapiens, GI13540606, Length=298, Percent_Identity=34.8993288590604, Blast_Score=161, Evalue=3e-39,
Organism=Escherichia coli, GI1788943, Length=726, Percent_Identity=41.3223140495868, Blast_Score=518, Evalue=1e-148,
Organism=Escherichia coli, GI1787109, Length=321, Percent_Identity=39.8753894080997, Blast_Score=233, Evalue=4e-62,
Organism=Saccharomyces cerevisiae, GI6320464, Length=687, Percent_Identity=41.6302765647744, Blast_Score=483, Evalue=1e-137,
Organism=Saccharomyces cerevisiae, GI6323002, Length=702, Percent_Identity=36.1823361823362, Blast_Score=433, Evalue=1e-122,

Paralogues:

None

Copy number: 560 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013093
- InterPro:   IPR003959
- InterPro:   IPR017729
- InterPro:   IPR018368
- InterPro:   IPR001270
- InterPro:   IPR019489
- InterPro:   IPR023150 [H]

Pfam domain/function: PF00004 AAA; PF07724 AAA_2; PF10431 ClpB_D2-small [H]

EC number: NA

Molecular weight: Translated: 97074; Mature: 96943

Theoretical pI: Translated: 5.32; Mature: 5.32

Prosite motif: PS00870 CLPAB_1 ; PS00871 CLPAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAITRKHLFGKLDATLFKGIESATTICKLRGNPYVELVHWLNQLWHQEDNDLKQIIRYFA
CCCCHHHHHHHHHHHHHHHHHHHHHEEEECCCCHHHHHHHHHHHHCCCCHHHHHHHHHHH
VDVDAFERELAQALAKLPVGATSISDFSYHIELATERAWIYASLECLDTRIRSGHLLLAL
HCHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEECCEEEEEHHHHHHHHHCCCHHHHHH
LTTMELRRAFFAIAPSMEKIPLEHLSKDLNFITQASPEANEAASDGSPLYDGALPGEASN
HHHHHHHHHHHHHCCCHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCC
AIGAAKSGGTLAQYTTDLTALAREGKIDPVLGRNHEINTMVDILLRRRQNNPLLTGDAGV
CCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEECCCCC
GKTAIVEGLALAIAAGSMPPALSQVSLLSLDIVALSAGASMKGEFEARLKSVLDEAIAAE
CHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHEECCCCCCCHHHHHHHHHHHHHHHCC
KPVILFIDEVHTLIGAGGNAGTGDAANLLKPALARGQLRTIGATTWSEFKRHIEKDPALT
CCEEEEHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCEECCHHHHHHHHHHHCCHHHH
RRFQVLQVDEPDENTAISMLRGLIPALEKHHGVWIMDEALQAAVRLSHRYIPARQLPDKA
HHEEEEECCCCCCHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHCCCHHHCCHHH
ISLLDTACARIAVAQHSQPIELQHLTFQSETAQTELVSLEKARHFGKAQDARTEQLKTSI
HHHHHHHHHHHHHHCCCCCCEEEEEEECCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
AEHGEAADKLDQRWQAERELVSAITTIRTALYDLVSQPEPDEEKRRAYQAQLVQLEAQLS
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
QVRTSLPLVQTEVNAEVIASIVADWTGIPVGQMLKDDIRAVMELPQRLEARVIGQPHALM
HHHHHCCHHEECCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
QLGENIMTARAGLSDPRKPLGVFMLVGPSGVGKTETALAIAESMYGGEQNMITINMSEYQ
HHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCCCEEEEEHHHHH
ESHTVSSLKGSPPGYVGYGEGGVLTEAVRRKPYSVVLLDEIEKAHSDVHELFFQVFDKGQ
HHHHHHHCCCCCCCEEECCCCCHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCC
MEDGEGRFIDFKNTILLLTSNVGSELLSNLLADPDTAPDQDGILTALQPELLKVFPAAFL
CCCCCCCEEEECCEEEEEECHHHHHHHHHHHCCCCCCCCCCCEEHHCCHHHHHHHHHHHH
GRVTVIPYLPLQQDALQHIVRLHLDRIGARLYSQHQLTLKYSDEVVDDVVSRCAVSETGA
CCHHEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEECHHHHHHHHHHHHHHHHHH
RMLIRYIEQNITPKIGKYILGDSDVKPEQIIFVHKNENGFVIALQ
HHHHHHHHHCCCHHHHHEEECCCCCCCCEEEEEEECCCCEEEEEC
>Mature Secondary Structure 
AITRKHLFGKLDATLFKGIESATTICKLRGNPYVELVHWLNQLWHQEDNDLKQIIRYFA
CCCHHHHHHHHHHHHHHHHHHHHHEEEECCCCHHHHHHHHHHHHCCCCHHHHHHHHHHH
VDVDAFERELAQALAKLPVGATSISDFSYHIELATERAWIYASLECLDTRIRSGHLLLAL
HCHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEECCEEEEEHHHHHHHHHCCCHHHHHH
LTTMELRRAFFAIAPSMEKIPLEHLSKDLNFITQASPEANEAASDGSPLYDGALPGEASN
HHHHHHHHHHHHHCCCHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCC
AIGAAKSGGTLAQYTTDLTALAREGKIDPVLGRNHEINTMVDILLRRRQNNPLLTGDAGV
CCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEECCCCC
GKTAIVEGLALAIAAGSMPPALSQVSLLSLDIVALSAGASMKGEFEARLKSVLDEAIAAE
CHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHEECCCCCCCHHHHHHHHHHHHHHHCC
KPVILFIDEVHTLIGAGGNAGTGDAANLLKPALARGQLRTIGATTWSEFKRHIEKDPALT
CCEEEEHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCEECCHHHHHHHHHHHCCHHHH
RRFQVLQVDEPDENTAISMLRGLIPALEKHHGVWIMDEALQAAVRLSHRYIPARQLPDKA
HHEEEEECCCCCCHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHCCCHHHCCHHH
ISLLDTACARIAVAQHSQPIELQHLTFQSETAQTELVSLEKARHFGKAQDARTEQLKTSI
HHHHHHHHHHHHHHCCCCCCEEEEEEECCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
AEHGEAADKLDQRWQAERELVSAITTIRTALYDLVSQPEPDEEKRRAYQAQLVQLEAQLS
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
QVRTSLPLVQTEVNAEVIASIVADWTGIPVGQMLKDDIRAVMELPQRLEARVIGQPHALM
HHHHHCCHHEECCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
QLGENIMTARAGLSDPRKPLGVFMLVGPSGVGKTETALAIAESMYGGEQNMITINMSEYQ
HHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCCCEEEEEHHHHH
ESHTVSSLKGSPPGYVGYGEGGVLTEAVRRKPYSVVLLDEIEKAHSDVHELFFQVFDKGQ
HHHHHHHCCCCCCCEEECCCCCHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCC
MEDGEGRFIDFKNTILLLTSNVGSELLSNLLADPDTAPDQDGILTALQPELLKVFPAAFL
CCCCCCCEEEECCEEEEEECHHHHHHHHHHHCCCCCCCCCCCEEHHCCHHHHHHHHHHHH
GRVTVIPYLPLQQDALQHIVRLHLDRIGARLYSQHQLTLKYSDEVVDDVVSRCAVSETGA
CCHHEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEECHHHHHHHHHHHHHHHHHH
RMLIRYIEQNITPKIGKYILGDSDVKPEQIIFVHKNENGFVIALQ
HHHHHHHHHCCCHHHHHEEECCCCCCCCEEEEEEECCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases); Serine endopeptidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10986262 [H]