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The map label for this gene is purN [H]

Identifier: 170023592

GI number: 170023592

Start: 1490866

End: 1491504

Strand: Reverse

Name: purN [H]

Synonym: YPK_1350

Alternate gene names: 170023592

Gene position: 1491504-1490866 (Counterclockwise)

Preceding gene: 170023593

Following gene: 170023591

Centisome position: 31.81

GC content: 50.86

Gene sequence:

>639_bases
ATGAAAAAAATTGTCGTATTAATTTCAGGCCAGGGAAGTAATTTACAAGCACTCATCGATGCCCAACAACAAGGGCGTAT
CTCTGGCAAGATCAGCGCTGTATTCAGCAATAATCCTGCAGCCTATGGTTTGGAACGTGCTGAATCCGCAGGGATCCCTC
ATCATGCGCTGGATGCCAAGGCTTTTACTGACCGCGTCAGTTTTGATCTGGCTCTGGCACAGGCCATCGACCAATATCAA
CCTGATTTATTGGTCCTCGCGGGTTATATGCGTATTCTCAGTCCAGAATTTGTTAAACACTATGCTGGGCGAATGTTGAA
TATTCACCCCTCGCTGTTGCCCAAATATCCAGGGCTGCATACCCACCGCCAGGCACTCGAAAACGGTGATCAAGAGCATG
GTACTTCTGTGCATTTTGTCACTGAAGAGTTGGATGGCGGCCCGGTGATCCTGCAAGCCAAGGTGCCGATTTTCAGCGAT
GACAGCGAAGAAGATGTGGCGGAACGGGTGCAAACCCAAGAGCACAGCATTTATCCGTTGGTCGTCAGTTGGTTTACCGA
TGGCCGCCTGGCAATGCGTGACAACGCGGCCTGGCTGGATGGGGTGCGTTTGCCGTTGCAGGGTTATGCTGCCGAATAA

Upstream 100 bases:

>100_bases
CATATTGTTCGGCAAGGCGGCCTCGCCTCTGCGAGGCCCACCGATGGATCGGGTAAGTGAACATAGCTAACCACCCTGCA
GCTTCAAGTACGAAGGGTAT

Downstream 100 bases:

>100_bases
CCTTCCCTTCGTACTTGAAGTCGCATAAATACTGTTCAAACGGCTTCCCGGCTAATGTGTCTCTCAGTGACCCCTTCCTG
CAACTCTCCAATGACGTTGG

Product: phosphoribosylglycinamide formyltransferase

Products: NA

Alternate protein names: 5'-phosphoribosylglycinamide transformylase; GAR transformylase; GART [H]

Number of amino acids: Translated: 212; Mature: 212

Protein sequence:

>212_residues
MKKIVVLISGQGSNLQALIDAQQQGRISGKISAVFSNNPAAYGLERAESAGIPHHALDAKAFTDRVSFDLALAQAIDQYQ
PDLLVLAGYMRILSPEFVKHYAGRMLNIHPSLLPKYPGLHTHRQALENGDQEHGTSVHFVTEELDGGPVILQAKVPIFSD
DSEEDVAERVQTQEHSIYPLVVSWFTDGRLAMRDNAAWLDGVRLPLQGYAAE

Sequences:

>Translated_212_residues
MKKIVVLISGQGSNLQALIDAQQQGRISGKISAVFSNNPAAYGLERAESAGIPHHALDAKAFTDRVSFDLALAQAIDQYQ
PDLLVLAGYMRILSPEFVKHYAGRMLNIHPSLLPKYPGLHTHRQALENGDQEHGTSVHFVTEELDGGPVILQAKVPIFSD
DSEEDVAERVQTQEHSIYPLVVSWFTDGRLAMRDNAAWLDGVRLPLQGYAAE
>Mature_212_residues
MKKIVVLISGQGSNLQALIDAQQQGRISGKISAVFSNNPAAYGLERAESAGIPHHALDAKAFTDRVSFDLALAQAIDQYQ
PDLLVLAGYMRILSPEFVKHYAGRMLNIHPSLLPKYPGLHTHRQALENGDQEHGTSVHFVTEELDGGPVILQAKVPIFSD
DSEEDVAERVQTQEHSIYPLVVSWFTDGRLAMRDNAAWLDGVRLPLQGYAAE

Specific function: De novo purine biosynthesis; third step. [C]

COG id: COG0299

COG function: function code F; Folate-dependent phosphoribosylglycinamide formyltransferase PurN

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GART family [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=199, Percent_Identity=43.7185929648241, Blast_Score=181, Evalue=5e-46,
Organism=Homo sapiens, GI209869995, Length=199, Percent_Identity=43.7185929648241, Blast_Score=181, Evalue=5e-46,
Organism=Homo sapiens, GI209869993, Length=199, Percent_Identity=43.7185929648241, Blast_Score=181, Evalue=5e-46,
Organism=Escherichia coli, GI1788846, Length=210, Percent_Identity=71.9047619047619, Blast_Score=320, Evalue=5e-89,
Organism=Escherichia coli, GI1787483, Length=165, Percent_Identity=35.1515151515151, Blast_Score=103, Evalue=7e-24,
Organism=Caenorhabditis elegans, GI17567511, Length=177, Percent_Identity=40.1129943502825, Blast_Score=135, Evalue=2e-32,
Organism=Saccharomyces cerevisiae, GI6320616, Length=199, Percent_Identity=34.6733668341709, Blast_Score=98, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24582400, Length=190, Percent_Identity=47.3684210526316, Blast_Score=181, Evalue=3e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR001555
- InterPro:   IPR004607 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =2.1.2.2 [H]

Molecular weight: Translated: 23310; Mature: 23310

Theoretical pI: Translated: 5.50; Mature: 5.50

Prosite motif: PS00373 GART

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKIVVLISGQGSNLQALIDAQQQGRISGKISAVFSNNPAAYGLERAESAGIPHHALDAK
CCEEEEEEECCCCCEEEEEECHHCCCCCEEEEEEECCCCCCCCCHHHHHCCCCCHHHCHH
AFTDRVSFDLALAQAIDQYQPDLLVLAGYMRILSPEFVKHYAGRMLNIHPSLLPKYPGLH
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCHHHHHHHHCCEEEECHHHCCCCCCCH
THRQALENGDQEHGTSVHFVTEELDGGPVILQAKVPIFSDDSEEDVAERVQTQEHSIYPL
HHHHHHHCCCCHHCCEEEEEEEECCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCEEEE
VVSWFTDGRLAMRDNAAWLDGVRLPLQGYAAE
EEEEECCCEEEEECCCCEECCEECCCCCCCCC
>Mature Secondary Structure
MKKIVVLISGQGSNLQALIDAQQQGRISGKISAVFSNNPAAYGLERAESAGIPHHALDAK
CCEEEEEEECCCCCEEEEEECHHCCCCCEEEEEEECCCCCCCCCHHHHHCCCCCHHHCHH
AFTDRVSFDLALAQAIDQYQPDLLVLAGYMRILSPEFVKHYAGRMLNIHPSLLPKYPGLH
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCHHHHHHHHCCEEEECHHHCCCCCCCH
THRQALENGDQEHGTSVHFVTEELDGGPVILQAKVPIFSDDSEEDVAERVQTQEHSIYPL
HHHHHHHCCCCHHCCEEEEEEEECCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCEEEE
VVSWFTDGRLAMRDNAAWLDGVRLPLQGYAAE
EEEEECCCEEEEECCCCEECCEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3301838; 9205837; 9278503; 10954745; 2204419; 1522592; 1631098; 9698564; 10606510 [H]